{"id":"https://openalex.org/W2086851259","doi":"https://doi.org/10.1093/nar/gki131","title":"HuSiDa--the human siRNA database: an open-access database for published functional siRNA sequences and technical details of efficient transfer into recipient cells","display_name":"HuSiDa--the human siRNA database: an open-access database for published functional siRNA sequences and technical details of efficient transfer into recipient cells","publication_year":2004,"publication_date":"2004-12-17","ids":{"openalex":"https://openalex.org/W2086851259","doi":"https://doi.org/10.1093/nar/gki131","mag":"2086851259","pmid":"https://pubmed.ncbi.nlm.nih.gov/15608157"},"language":"en","primary_location":{"id":"doi:10.1093/nar/gki131","is_oa":true,"landing_page_url":"https://doi.org/10.1093/nar/gki131","pdf_url":"https://academic.oup.com/nar/article-pdf/33/suppl_1/D108/7622498/gki131.pdf","source":{"id":"https://openalex.org/S134668137","display_name":"Nucleic Acids Research","issn_l":"0305-1048","issn":["0305-1048","1362-4954","1362-4962"],"is_oa":false,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic Acids Research","raw_type":"journal-article"},"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"https://academic.oup.com/nar/article-pdf/33/suppl_1/D108/7622498/gki131.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5111557343","display_name":"Mathias Truss","orcid":null},"institutions":[{"id":"https://openalex.org/I7877124","display_name":"Charit\u00e9 - Universit\u00e4tsmedizin Berlin","ror":"https://ror.org/001w7jn25","country_code":"DE","type":"healthcare","lineage":["https://openalex.org/I7877124"]}],"countries":["DE"],"is_corresponding":true,"raw_author_name":"M. Truss","raw_affiliation_strings":["Department of Pediatrics, Laboratory for Molecular Biology, Institute of Pathology, Charit\u00e9, Universit\u00e4tsmedizin-Berlin, Germany. Matthias.Truss@Charite.de","Department of Pediatrics, Laboratory for Molecular Biology, Institute of Pathology, Charit\u00e9, Universit\u00e4tsmedizin-Berlin, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pediatrics, Laboratory for Molecular Biology, Institute of Pathology, Charit\u00e9, Universit\u00e4tsmedizin-Berlin, Germany. Matthias.Truss@Charite.de","institution_ids":["https://openalex.org/I7877124"]},{"raw_affiliation_string":"Department of Pediatrics, Laboratory for Molecular Biology, Institute of Pathology, Charit\u00e9, Universit\u00e4tsmedizin-Berlin, Germany","institution_ids":["https://openalex.org/I7877124"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5111557343"],"corresponding_institution_ids":["https://openalex.org/I7877124"],"apc_list":{"value":3630,"currency":"USD","value_usd":3630},"apc_paid":{"value":3630,"currency":"USD","value_usd":3630},"fwci":2.0405,"has_fulltext":true,"cited_by_count":50,"citation_normalized_percentile":{"value":0.86069756,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":98},"biblio":{"volume":"33","issue":"Database issue","first_page":"D108","last_page":"D111"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10725","display_name":"RNA Interference and Gene Delivery","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10725","display_name":"RNA Interference and Gene Delivery","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10207","display_name":"Advanced biosensing and bioanalysis techniques","score":0.9997000098228455,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10062","display_name":"MicroRNA in disease regulation","score":0.996399998664856,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/gene-silencing","display_name":"Gene silencing","score":0.8138383626937866},{"id":"https://openalex.org/keywords/small-interfering-rna","display_name":"Small interfering RNA","score":0.7657338380813599},{"id":"https://openalex.org/keywords/biology","display_name":"Biology","score":0.7005022168159485},{"id":"https://openalex.org/keywords/rna-interference","display_name":"RNA interference","score":0.6744817495346069},{"id":"https://openalex.org/keywords/database","display_name":"Database","score":0.6406207084655762},{"id":"https://openalex.org/keywords/trans-acting-sirna","display_name":"Trans-acting siRNA","score":0.6192851066589355},{"id":"https://openalex.org/keywords/functional-genomics","display_name":"Functional genomics","score":0.614082396030426},{"id":"https://openalex.org/keywords/computational-biology","display_name":"Computational biology","score":0.4888386130332947},{"id":"https://openalex.org/keywords/transfection","display_name":"Transfection","score":0.4211779236793518},{"id":"https://openalex.org/keywords/gene","display_name":"Gene","score":0.3900619149208069},{"id":"https://openalex.org/keywords/rna","display_name":"RNA","score":0.3073344826698303},{"id":"https://openalex.org/keywords/genetics","display_name":"Genetics","score":0.28264015913009644},{"id":"https://openalex.org/keywords/computer-science","display_name":"Computer science","score":0.1798059046268463},{"id":"https://openalex.org/keywords/genomics","display_name":"Genomics","score":0.16880986094474792},{"id":"https://openalex.org/keywords/genome","display_name":"Genome","score":0.0894341766834259}],"concepts":[{"id":"https://openalex.org/C119056186","wikidata":"https://www.wikidata.org/wiki/Q1431332","display_name":"Gene silencing","level":3,"score":0.8138383626937866},{"id":"https://openalex.org/C22615655","wikidata":"https://www.wikidata.org/wiki/Q203221","display_name":"Small interfering RNA","level":4,"score":0.7657338380813599},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7005022168159485},{"id":"https://openalex.org/C166703698","wikidata":"https://www.wikidata.org/wiki/Q201993","display_name":"RNA interference","level":4,"score":0.6744817495346069},{"id":"https://openalex.org/C77088390","wikidata":"https://www.wikidata.org/wiki/Q8513","display_name":"Database","level":1,"score":0.6406207084655762},{"id":"https://openalex.org/C60723119","wikidata":"https://www.wikidata.org/wiki/Q7833577","display_name":"Trans-acting siRNA","level":5,"score":0.6192851066589355},{"id":"https://openalex.org/C161078062","wikidata":"https://www.wikidata.org/wiki/Q1068690","display_name":"Functional genomics","level":5,"score":0.614082396030426},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.4888386130332947},{"id":"https://openalex.org/C54009773","wikidata":"https://www.wikidata.org/wiki/Q1429031","display_name":"Transfection","level":3,"score":0.4211779236793518},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.3900619149208069},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.3073344826698303},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.28264015913009644},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.1798059046268463},{"id":"https://openalex.org/C189206191","wikidata":"https://www.wikidata.org/wiki/Q222046","display_name":"Genomics","level":4,"score":0.16880986094474792},{"id":"https://openalex.org/C141231307","wikidata":"https://www.wikidata.org/wiki/Q7020","display_name":"Genome","level":3,"score":0.0894341766834259}],"mesh":[{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014162","descriptor_name":"Transfection","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014162","descriptor_name":"Transfection","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014162","descriptor_name":"Transfection","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D030561","descriptor_name":"Databases, Nucleic Acid","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D030561","descriptor_name":"Databases, Nucleic Acid","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D030561","descriptor_name":"Databases, Nucleic Acid","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D034622","descriptor_name":"RNA Interference","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D034622","descriptor_name":"RNA Interference","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D034622","descriptor_name":"RNA Interference","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D034741","descriptor_name":"RNA, Small Interfering","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D034741","descriptor_name":"RNA, Small Interfering","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D034741","descriptor_name":"RNA, Small Interfering","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D034741","descriptor_name":"RNA, Small Interfering","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D034741","descriptor_name":"RNA, Small Interfering","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D034741","descriptor_name":"RNA, Small Interfering","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false}],"locations_count":4,"locations":[{"id":"doi:10.1093/nar/gki131","is_oa":true,"landing_page_url":"https://doi.org/10.1093/nar/gki131","pdf_url":"https://academic.oup.com/nar/article-pdf/33/suppl_1/D108/7622498/gki131.pdf","source":{"id":"https://openalex.org/S134668137","display_name":"Nucleic Acids Research","issn_l":"0305-1048","issn":["0305-1048","1362-4954","1362-4962"],"is_oa":false,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic Acids Research","raw_type":"journal-article"},{"id":"pmid:15608157","is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/15608157","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic acids research","raw_type":null},{"id":"pmh:oai:europepmc.org:163571","is_oa":false,"landing_page_url":"http://europepmc.org/pmc/articles/PMC540085","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Text"},{"id":"pmh:oai:pubmedcentral.nih.gov:540085","is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/540085","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Nucleic Acids Res","raw_type":"Text"}],"best_oa_location":{"id":"doi:10.1093/nar/gki131","is_oa":true,"landing_page_url":"https://doi.org/10.1093/nar/gki131","pdf_url":"https://academic.oup.com/nar/article-pdf/33/suppl_1/D108/7622498/gki131.pdf","source":{"id":"https://openalex.org/S134668137","display_name":"Nucleic Acids Research","issn_l":"0305-1048","issn":["0305-1048","1362-4954","1362-4962"],"is_oa":false,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic Acids Research","raw_type":"journal-article"},"sustainable_development_goals":[{"score":0.6700000166893005,"display_name":"Decent work and economic growth","id":"https://metadata.un.org/sdg/8"}],"awards":[],"funders":[{"id":"https://openalex.org/F4320320879","display_name":"Deutsche Forschungsgemeinschaft","ror":"https://ror.org/018mejw64"},{"id":"https://openalex.org/F4320321114","display_name":"Bundesministerium f\u00fcr Bildung und Forschung","ror":"https://ror.org/04pz7b180"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2086851259.pdf","grobid_xml":"https://content.openalex.org/works/W2086851259.grobid-xml"},"referenced_works_count":20,"referenced_works":["https://openalex.org/W1611419331","https://openalex.org/W1974861468","https://openalex.org/W1996164865","https://openalex.org/W2017642141","https://openalex.org/W2021310770","https://openalex.org/W2038783205","https://openalex.org/W2044396442","https://openalex.org/W2071596792","https://openalex.org/W2095674416","https://openalex.org/W2097261962","https://openalex.org/W2099915551","https://openalex.org/W2109609799","https://openalex.org/W2131230706","https://openalex.org/W2137553623","https://openalex.org/W2149538815","https://openalex.org/W2153747430","https://openalex.org/W2166802510","https://openalex.org/W4230260023","https://openalex.org/W4238528463","https://openalex.org/W4251586319"],"related_works":["https://openalex.org/W1966759115","https://openalex.org/W2408799732","https://openalex.org/W1969367341","https://openalex.org/W2031645836","https://openalex.org/W2408505947","https://openalex.org/W47528747","https://openalex.org/W2062595050","https://openalex.org/W2438003748","https://openalex.org/W63115930","https://openalex.org/W1967317528"],"abstract_inverted_index":{"Small":[0],"interfering":[1],"RNAs":[2],"(siRNAs)":[3],"have":[4,92],"become":[5],"a":[6,34],"standard":[7],"tool":[8],"in":[9,155],"functional":[10,105],"genomics.":[11],"Once":[12],"incorporated":[13],"into":[14],"the":[15,22,60,71,84,94,116,122,150,156],"RNA-induced":[16],"silencing":[17,119],"complex":[18],"(RISC),":[19],"siRNAs":[20],"mediate":[21],"specific":[23],"recognition":[24],"of":[25,37,65,73,88,103,115,124],"corresponding":[26,117],"target":[27,74],"mRNAs":[28],"and":[29,62,70,86,111,132,134],"their":[30],"cleavage.":[31],"However,":[32],"only":[33],"small":[35],"fraction":[36],"randomly":[38],"chosen":[39],"siRNA":[40,68,80,89,96,106,125,151],"sequences":[41,102],"is":[42],"able":[43],"to":[44,137],"induce":[45],"efficient":[46,165],"gene":[47,118,166],"silencing.":[48,167],"In":[49],"common":[50],"laboratory":[51],"practice,":[52],"successful":[53],"RNA":[54],"interference":[55],"experiments":[56],"typically":[57],"require":[58],"both,":[59],"labour":[61],"cost-intensive":[63],"identification":[64],"an":[66],"active":[67],"sequence":[69,152,161],"optimization":[72],"cell":[75,128],"line-specific":[76],"procedures":[77,133],"for":[78,158,164],"optimal":[79],"delivery.":[81],"To":[82],"optimize":[83],"design":[85],"performance":[87],"experiments,":[90,120],"we":[91],"established":[93],"human":[95,109],"database":[97,100,142,157],"(HuSiDa).":[98],"The":[99,141],"provides":[101],"published":[104,138,160],"molecules":[107],"targeting":[108],"genes":[110],"important":[112],"technical":[113],"details":[114],"including":[121],"mode":[123],"generation,":[126],"recipient":[127],"lines,":[129],"transfection":[130],"reagents":[131],"direct":[135],"links":[136],"references":[139],"(PubMed).":[140],"can":[143],"be":[144],"accessed":[145],"at":[146],"http://www.human-siRNA-database.net.":[147],"We":[148],"used":[149],"information":[153],"stored":[154],"scrutinizing":[159],"selection":[162],"parameters":[163]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2023,"cited_by_count":1},{"year":2021,"cited_by_count":1},{"year":2019,"cited_by_count":1},{"year":2018,"cited_by_count":2},{"year":2017,"cited_by_count":2},{"year":2016,"cited_by_count":4},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":2},{"year":2013,"cited_by_count":1},{"year":2012,"cited_by_count":2}],"updated_date":"2026-07-22T07:51:19.307946","created_date":"2025-10-10T00:00:00"}