{"id":"https://openalex.org/W2002581509","doi":"https://doi.org/10.1093/nar/gkf399","title":"Non-contact positions impose site selectivity on Cre recombinase","display_name":"Non-contact positions impose site selectivity on Cre recombinase","publication_year":2002,"publication_date":"2002-07-01","ids":{"openalex":"https://openalex.org/W2002581509","doi":"https://doi.org/10.1093/nar/gkf399","mag":"2002581509","pmid":"https://pubmed.ncbi.nlm.nih.gov/12087159"},"language":"en","primary_location":{"id":"doi:10.1093/nar/gkf399","is_oa":true,"landing_page_url":"https://doi.org/10.1093/nar/gkf399","pdf_url":"https://academic.oup.com/nar/article-pdf/30/13/2764/7040795/gkf399.pdf","source":{"id":"https://openalex.org/S134668137","display_name":"Nucleic Acids Research","issn_l":"0305-1048","issn":["0305-1048","1362-4954","1362-4962"],"is_oa":false,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic Acids Research","raw_type":"journal-article"},"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://academic.oup.com/nar/article-pdf/30/13/2764/7040795/gkf399.pdf","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5041611636","display_name":"Andreas R\u00fcfer","orcid":null},"institutions":[{"id":"https://openalex.org/I114131015","display_name":"Stowers Institute for Medical Research","ror":"https://ror.org/04bgfm609","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I114131015"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"A. W. Rufer","raw_affiliation_strings":["Stowers Institute for Medical Research, 1000 East 50th Street, Kansas City, MO 64110, USA","Stowers Institute for Medical Research  1000 East 50th Street, Kansas City, MO 64110, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Stowers Institute for Medical Research, 1000 East 50th Street, Kansas City, MO 64110, USA","institution_ids":["https://openalex.org/I114131015"]},{"raw_affiliation_string":"Stowers Institute for Medical Research  1000 East 50th Street, Kansas City, MO 64110, USA","institution_ids":["https://openalex.org/I114131015"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5041611636"],"corresponding_institution_ids":["https://openalex.org/I114131015"],"apc_list":{"value":3630,"currency":"USD","value_usd":3630},"apc_paid":null,"fwci":2.4692,"has_fulltext":false,"cited_by_count":45,"citation_normalized_percentile":{"value":0.88919551,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":90,"max":98},"biblio":{"volume":"30","issue":"13","first_page":"2764","last_page":"2771"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.9997000098228455,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.9997000098228455,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.9997000098228455,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10207","display_name":"Advanced biosensing and bioanalysis techniques","score":0.9991000294685364,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/biology","display_name":"Biology","score":0.8254157900810242},{"id":"https://openalex.org/keywords/recombinase","display_name":"Recombinase","score":0.7372451424598694},{"id":"https://openalex.org/keywords/cre-recombinase","display_name":"Cre recombinase","score":0.7365971803665161},{"id":"https://openalex.org/keywords/recombination","display_name":"Recombination","score":0.6522414088249207},{"id":"https://openalex.org/keywords/flp-frt-recombination","display_name":"FLP-FRT recombination","score":0.6456816792488098},{"id":"https://openalex.org/keywords/dna-shuffling","display_name":"DNA shuffling","score":0.6282370090484619},{"id":"https://openalex.org/keywords/dna","display_name":"DNA","score":0.6018575429916382},{"id":"https://openalex.org/keywords/mutant","display_name":"Mutant","score":0.5886018872261047},{"id":"https://openalex.org/keywords/mutagenesis","display_name":"Mutagenesis","score":0.558117687702179},{"id":"https://openalex.org/keywords/genetics","display_name":"Genetics","score":0.5483381152153015},{"id":"https://openalex.org/keywords/base-pair","display_name":"Base pair","score":0.47839319705963135},{"id":"https://openalex.org/keywords/molecular-biology","display_name":"Molecular biology","score":0.4590955376625061},{"id":"https://openalex.org/keywords/site-directed-mutagenesis","display_name":"Site-directed mutagenesis","score":0.45052605867385864},{"id":"https://openalex.org/keywords/cre-lox-recombination","display_name":"Cre-Lox recombination","score":0.43172410130500793},{"id":"https://openalex.org/keywords/in-vitro-recombination","display_name":"In vitro recombination","score":0.4130942225456238},{"id":"https://openalex.org/keywords/directed-evolution","display_name":"Directed evolution","score":0.2727382779121399},{"id":"https://openalex.org/keywords/gene","display_name":"Gene","score":0.20674794912338257},{"id":"https://openalex.org/keywords/genetic-recombination","display_name":"Genetic recombination","score":0.20188313722610474},{"id":"https://openalex.org/keywords/transgene","display_name":"Transgene","score":0.10916927456855774},{"id":"https://openalex.org/keywords/peptide-sequence","display_name":"Peptide sequence","score":0.09713104367256165},{"id":"https://openalex.org/keywords/molecular-cloning","display_name":"Molecular cloning","score":0.07969814538955688}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.8254157900810242},{"id":"https://openalex.org/C102414288","wikidata":"https://www.wikidata.org/wiki/Q1172461","display_name":"Recombinase","level":4,"score":0.7372451424598694},{"id":"https://openalex.org/C125929170","wikidata":"https://www.wikidata.org/wiki/Q908966","display_name":"Cre recombinase","level":5,"score":0.7365971803665161},{"id":"https://openalex.org/C156695909","wikidata":"https://www.wikidata.org/wiki/Q3373825","display_name":"Recombination","level":3,"score":0.6522414088249207},{"id":"https://openalex.org/C135300049","wikidata":"https://www.wikidata.org/wiki/Q5426559","display_name":"FLP-FRT recombination","level":5,"score":0.6456816792488098},{"id":"https://openalex.org/C2779454882","wikidata":"https://www.wikidata.org/wiki/Q5205776","display_name":"DNA shuffling","level":5,"score":0.6282370090484619},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.6018575429916382},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.5886018872261047},{"id":"https://openalex.org/C16318435","wikidata":"https://www.wikidata.org/wiki/Q149299","display_name":"Mutagenesis","level":4,"score":0.558117687702179},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.5483381152153015},{"id":"https://openalex.org/C185581394","wikidata":"https://www.wikidata.org/wiki/Q145911","display_name":"Base pair","level":3,"score":0.47839319705963135},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.4590955376625061},{"id":"https://openalex.org/C103408237","wikidata":"https://www.wikidata.org/wiki/Q2642668","display_name":"Site-directed mutagenesis","level":4,"score":0.45052605867385864},{"id":"https://openalex.org/C38027674","wikidata":"https://www.wikidata.org/wiki/Q4994856","display_name":"Cre-Lox recombination","level":5,"score":0.43172410130500793},{"id":"https://openalex.org/C203284983","wikidata":"https://www.wikidata.org/wiki/Q17087215","display_name":"In vitro recombination","level":5,"score":0.4130942225456238},{"id":"https://openalex.org/C9418097","wikidata":"https://www.wikidata.org/wiki/Q440681","display_name":"Directed evolution","level":4,"score":0.2727382779121399},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.20674794912338257},{"id":"https://openalex.org/C101434241","wikidata":"https://www.wikidata.org/wiki/Q211675","display_name":"Genetic recombination","level":4,"score":0.20188313722610474},{"id":"https://openalex.org/C102230213","wikidata":"https://www.wikidata.org/wiki/Q430117","display_name":"Transgene","level":3,"score":0.10916927456855774},{"id":"https://openalex.org/C167625842","wikidata":"https://www.wikidata.org/wiki/Q899763","display_name":"Peptide sequence","level":3,"score":0.09713104367256165},{"id":"https://openalex.org/C19924922","wikidata":"https://www.wikidata.org/wiki/Q408351","display_name":"Molecular cloning","level":4,"score":0.07969814538955688},{"id":"https://openalex.org/C141035611","wikidata":"https://www.wikidata.org/wiki/Q5532934","display_name":"Genetically modified mouse","level":4,"score":0.0}],"mesh":[{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001665","descriptor_name":"Binding Sites","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D001665","descriptor_name":"Binding Sites","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D001665","descriptor_name":"Binding Sites","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D004269","descriptor_name":"DNA, Bacterial","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004269","descriptor_name":"DNA, Bacterial","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004269","descriptor_name":"DNA, Bacterial","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004269","descriptor_name":"DNA, Bacterial","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D004269","descriptor_name":"DNA, Bacterial","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D004269","descriptor_name":"DNA, Bacterial","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D009154","descriptor_name":"Mutation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009154","descriptor_name":"Mutation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009154","descriptor_name":"Mutation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011485","descriptor_name":"Protein Binding","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011485","descriptor_name":"Protein Binding","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011485","descriptor_name":"Protein Binding","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011995","descriptor_name":"Recombination, Genetic","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D011995","descriptor_name":"Recombination, Genetic","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D011995","descriptor_name":"Recombination, Genetic","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D014764","descriptor_name":"Viral Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D014764","descriptor_name":"Viral Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D014764","descriptor_name":"Viral Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D014764","descriptor_name":"Viral Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D014764","descriptor_name":"Viral Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D014764","descriptor_name":"Viral Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D017386","descriptor_name":"Sequence Homology, Amino Acid","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017386","descriptor_name":"Sequence Homology, Amino Acid","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017386","descriptor_name":"Sequence Homology, Amino Acid","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019426","descriptor_name":"Integrases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D019426","descriptor_name":"Integrases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D019426","descriptor_name":"Integrases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D019426","descriptor_name":"Integrases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D019426","descriptor_name":"Integrases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D019426","descriptor_name":"Integrases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D024202","descriptor_name":"Electrophoretic Mobility Shift Assay","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D024202","descriptor_name":"Electrophoretic Mobility Shift Assay","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D024202","descriptor_name":"Electrophoretic Mobility Shift Assay","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":4,"locations":[{"id":"doi:10.1093/nar/gkf399","is_oa":true,"landing_page_url":"https://doi.org/10.1093/nar/gkf399","pdf_url":"https://academic.oup.com/nar/article-pdf/30/13/2764/7040795/gkf399.pdf","source":{"id":"https://openalex.org/S134668137","display_name":"Nucleic Acids Research","issn_l":"0305-1048","issn":["0305-1048","1362-4954","1362-4962"],"is_oa":false,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic Acids Research","raw_type":"journal-article"},{"id":"pmid:12087159","is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/12087159","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic acids research","raw_type":null},{"id":"pmh:oai:europepmc.org:155326","is_oa":false,"landing_page_url":"http://europepmc.org/pmc/articles/PMC117054","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Text"},{"id":"pmh:oai:europepmc.org:155326","is_oa":false,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/117054","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Text"}],"best_oa_location":{"id":"doi:10.1093/nar/gkf399","is_oa":true,"landing_page_url":"https://doi.org/10.1093/nar/gkf399","pdf_url":"https://academic.oup.com/nar/article-pdf/30/13/2764/7040795/gkf399.pdf","source":{"id":"https://openalex.org/S134668137","display_name":"Nucleic Acids Research","issn_l":"0305-1048","issn":["0305-1048","1362-4954","1362-4962"],"is_oa":false,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic Acids Research","raw_type":"journal-article"},"sustainable_development_goals":[{"score":0.5400000214576721,"display_name":"Peace, Justice and strong institutions","id":"https://metadata.un.org/sdg/16"},{"score":0.47999998927116394,"display_name":"Reduced inequalities","id":"https://metadata.un.org/sdg/10"}],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":false},"content_urls":{"pdf":"https://content.openalex.org/works/W2002581509.pdf"},"referenced_works_count":26,"referenced_works":["https://openalex.org/W122685959","https://openalex.org/W1495835504","https://openalex.org/W1512374553","https://openalex.org/W1581618630","https://openalex.org/W1590546489","https://openalex.org/W1678580492","https://openalex.org/W1976918496","https://openalex.org/W1980909118","https://openalex.org/W1998177092","https://openalex.org/W1999563094","https://openalex.org/W2011513747","https://openalex.org/W2015642465","https://openalex.org/W2018168127","https://openalex.org/W2020777893","https://openalex.org/W2038970416","https://openalex.org/W2047415109","https://openalex.org/W2057339816","https://openalex.org/W2060020224","https://openalex.org/W2061705914","https://openalex.org/W2086791288","https://openalex.org/W2094661180","https://openalex.org/W2095310293","https://openalex.org/W2119095571","https://openalex.org/W2138187877","https://openalex.org/W2160084442","https://openalex.org/W2163653659"],"related_works":["https://openalex.org/W2378394429","https://openalex.org/W3146917270","https://openalex.org/W2097759518","https://openalex.org/W2104975387","https://openalex.org/W2014892279","https://openalex.org/W1971665839","https://openalex.org/W1973703541","https://openalex.org/W1535510845","https://openalex.org/W1988309865","https://openalex.org/W2363480439"],"abstract_inverted_index":{"A":[0,115],"first":[1],"step":[2],"in":[3,46,50,55,101,108,135,150,162],"Cre-mediated":[4],"site-specific":[5],"DNA":[6,63,69,154],"recombination":[7,155,192],"is":[8],"binding":[9],"to":[10,77],"the":[11,17,30,97,127,157,177,186],"two":[12,36],"13":[13],"bp":[14,19],"repeats":[15],"of":[16,37,96,126,139,143,148,166,189,191],"34":[18],"site":[20,89],"loxP.":[21],"Several":[22],"nucleotides":[23],"within":[24],"loxP":[25,194],"do":[26],"not":[27,124],"directly":[28],"contact":[29],"bound":[31],"enzyme,":[32],"yet":[33],"mutation":[34,117],"at":[35,41,83,118,130,156,172,182,193],"these":[38,91,102],"base":[39],"pairs,":[40],"positions":[42],"11":[43],"and":[44,72,110,195],"12":[45],"each":[47],"repeat,":[48],"results":[49],"a":[51,73,86],"100":[52],"000-fold":[53],"reduction":[54],"recombination.":[56],"To":[57],"understand":[58],"better":[59],"how":[60],"Cre":[61,79,103,149],"selects":[62],"sequences":[64],"for":[65,121],"recombination,":[66],"we":[67],"combined":[68],"shuffling":[70],"mutagenesis":[71],"forward":[74],"selection":[75],"strategy":[76],"obtain":[78],"mutants":[80],"that":[81,176],"recombine":[82],"100%":[84],"efficiency":[85,190],"mutant":[87],"loxK2":[88,144],"carrying":[90],"dinucleotide":[92],"changes.":[93],"The":[94],"role":[95],"several":[98],"mutations":[99,133],"found":[100],"isolates":[104],"was":[105],"analyzed":[106],"both":[107],"vivo":[109,151],"biochemically":[111],"with":[112],"purified":[113],"enzymes.":[114],"single":[116],"E262":[119,174],"accounts":[120],"most":[122],"but":[123],"all":[125,167],"enhanced":[128],"activity":[129],"loxK2.":[131,163,199],"Secondary":[132],"act":[134],"one":[136],"or":[137,152],"more":[138],"three":[140],"ways:":[141],"enhancement":[142],"binding,":[145],"accelerated":[146],"synthesis":[147],"faster":[153],"alternative":[158],"spacer":[159],"region":[160],"present":[161],"Systematic":[164],"analysis":[165],"20":[168],"natural":[169],"amino":[170],"acids":[171],"position":[173,184],"shows":[175],"naturally":[178],"occurring":[179],"glutamate":[180],"residue":[181],"this":[183],"provides":[185],"optimal":[187],"balance":[188],"maximal":[196],"discrimination":[197],"against":[198]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":4},{"year":2020,"cited_by_count":6},{"year":2019,"cited_by_count":1},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":4},{"year":2012,"cited_by_count":1}],"updated_date":"2026-05-22T06:13:13.366637","created_date":"2025-10-10T00:00:00"}