{"id":"https://openalex.org/W3092284562","doi":"https://doi.org/10.1093/nar/gkaa848","title":"dbGuide: a database of functionally validated guide RNAs for genome editing in human and mouse cells","display_name":"dbGuide: a database of functionally validated guide RNAs for genome editing in human and mouse cells","publication_year":2020,"publication_date":"2020-09-22","ids":{"openalex":"https://openalex.org/W3092284562","doi":"https://doi.org/10.1093/nar/gkaa848","mag":"3092284562","pmid":"https://pubmed.ncbi.nlm.nih.gov/33051688"},"language":"en","primary_location":{"id":"doi:10.1093/nar/gkaa848","is_oa":true,"landing_page_url":"https://doi.org/10.1093/nar/gkaa848","pdf_url":"https://academic.oup.com/nar/article-pdf/49/D1/D871/35364779/gkaa848.pdf","source":{"id":"https://openalex.org/S134668137","display_name":"Nucleic Acids Research","issn_l":"0305-1048","issn":["0305-1048","1362-4954","1362-4962"],"is_oa":true,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic Acids Research","raw_type":"journal-article"},"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://academic.oup.com/nar/article-pdf/49/D1/D871/35364779/gkaa848.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5067390761","display_name":"Alexander A Gooden","orcid":null},"institutions":[{"id":"https://openalex.org/I4210130649","display_name":"Frederick National Laboratory for Cancer Research","ror":"https://ror.org/03v6m3209","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210130649","https://openalex.org/I4210140884"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Alexander A Gooden","raw_affiliation_strings":["Laboratory Animal Sciences Program, Frederick National Lab for Cancer Research, Frederick, MD 21702, USA"],"affiliations":[{"raw_affiliation_string":"Laboratory Animal Sciences Program, Frederick National Lab for Cancer Research, Frederick, MD 21702, USA","institution_ids":["https://openalex.org/I4210130649"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5071651888","display_name":"Christine N. Evans","orcid":null},"institutions":[{"id":"https://openalex.org/I4210130649","display_name":"Frederick National Laboratory for Cancer Research","ror":"https://ror.org/03v6m3209","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210130649","https://openalex.org/I4210140884"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Christine N Evans","raw_affiliation_strings":["Laboratory Animal Sciences Program, Frederick National Lab for Cancer Research, Frederick, MD 21702, USA"],"affiliations":[{"raw_affiliation_string":"Laboratory Animal Sciences Program, Frederick National Lab for Cancer Research, Frederick, MD 21702, USA","institution_ids":["https://openalex.org/I4210130649"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5043655377","display_name":"Timothy P. Sheets","orcid":"https://orcid.org/0000-0002-6602-2689"},"institutions":[{"id":"https://openalex.org/I4210130649","display_name":"Frederick National Laboratory for Cancer Research","ror":"https://ror.org/03v6m3209","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210130649","https://openalex.org/I4210140884"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Timothy P Sheets","raw_affiliation_strings":["Laboratory Animal Sciences Program, Frederick National Lab for Cancer Research, Frederick, MD 21702, USA"],"affiliations":[{"raw_affiliation_string":"Laboratory Animal Sciences Program, Frederick National Lab for Cancer Research, Frederick, MD 21702, USA","institution_ids":["https://openalex.org/I4210130649"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5035822406","display_name":"Michelle E. Clapp","orcid":null},"institutions":[{"id":"https://openalex.org/I4210130649","display_name":"Frederick National Laboratory for Cancer Research","ror":"https://ror.org/03v6m3209","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210130649","https://openalex.org/I4210140884"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Michelle E Clapp","raw_affiliation_strings":["Laboratory Animal Sciences Program, Frederick National Lab for Cancer Research, Frederick, MD 21702, USA"],"affiliations":[{"raw_affiliation_string":"Laboratory Animal Sciences Program, Frederick National Lab for Cancer Research, Frederick, MD 21702, USA","institution_ids":["https://openalex.org/I4210130649"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5056677073","display_name":"Raj Chari","orcid":"https://orcid.org/0000-0002-2216-313X"},"institutions":[{"id":"https://openalex.org/I4210130649","display_name":"Frederick National Laboratory for Cancer Research","ror":"https://ror.org/03v6m3209","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210130649","https://openalex.org/I4210140884"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Raj Chari","raw_affiliation_strings":["Laboratory Animal Sciences Program, Frederick National Lab for Cancer Research, Frederick, MD 21702, USA"],"affiliations":[{"raw_affiliation_string":"Laboratory Animal Sciences Program, Frederick National Lab for Cancer Research, Frederick, MD 21702, USA","institution_ids":["https://openalex.org/I4210130649"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":5,"corresponding_author_ids":["https://openalex.org/A5056677073"],"corresponding_institution_ids":["https://openalex.org/I4210130649"],"apc_list":{"value":3630,"currency":"USD","value_usd":3630},"apc_paid":{"value":3630,"currency":"USD","value_usd":3630},"fwci":1.5852,"has_fulltext":true,"cited_by_count":34,"citation_normalized_percentile":{"value":0.83542624,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":97,"max":99},"biblio":{"volume":"49","issue":"D1","first_page":"D871","last_page":"D876"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12610","display_name":"RNA regulation and disease","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":0.9994000196456909,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/biology","display_name":"Biology","score":0.9464696645736694},{"id":"https://openalex.org/keywords/genome","display_name":"Genome","score":0.6049517393112183},{"id":"https://openalex.org/keywords/computational-biology","display_name":"Computational biology","score":0.5447713732719421},{"id":"https://openalex.org/keywords/human-genome","display_name":"Human genome","score":0.48644256591796875},{"id":"https://openalex.org/keywords/genetics","display_name":"Genetics","score":0.4491722583770752},{"id":"https://openalex.org/keywords/base-sequence","display_name":"Base sequence","score":0.4119037389755249},{"id":"https://openalex.org/keywords/database","display_name":"Database","score":0.4046531021595001},{"id":"https://openalex.org/keywords/dna","display_name":"DNA","score":0.2740700840950012},{"id":"https://openalex.org/keywords/gene","display_name":"Gene","score":0.24861383438110352}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.9464696645736694},{"id":"https://openalex.org/C141231307","wikidata":"https://www.wikidata.org/wiki/Q7020","display_name":"Genome","level":3,"score":0.6049517393112183},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.5447713732719421},{"id":"https://openalex.org/C197077220","wikidata":"https://www.wikidata.org/wiki/Q720988","display_name":"Human genome","level":4,"score":0.48644256591796875},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.4491722583770752},{"id":"https://openalex.org/C3017666073","wikidata":"https://www.wikidata.org/wiki/Q1764062","display_name":"Base sequence","level":3,"score":0.4119037389755249},{"id":"https://openalex.org/C77088390","wikidata":"https://www.wikidata.org/wiki/Q8513","display_name":"Database","level":1,"score":0.4046531021595001},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.2740700840950012},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.24861383438110352},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.0}],"mesh":[{"descriptor_ui":"D000072669","descriptor_name":"Gene Editing","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D000072669","descriptor_name":"Gene Editing","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D000072669","descriptor_name":"Gene Editing","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D000072669","descriptor_name":"Gene Editing","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D000094704","descriptor_name":"RNA, Guide, CRISPR-Cas Systems","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D000094704","descriptor_name":"RNA, Guide, CRISPR-Cas Systems","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D000094704","descriptor_name":"RNA, Guide, CRISPR-Cas Systems","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002477","descriptor_name":"Cells","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D002477","descriptor_name":"Cells","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D002477","descriptor_name":"Cells","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D002477","descriptor_name":"Cells","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014584","descriptor_name":"User-Computer Interface","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014584","descriptor_name":"User-Computer Interface","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014584","descriptor_name":"User-Computer Interface","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014584","descriptor_name":"User-Computer Interface","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015203","descriptor_name":"Reproducibility of Results","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015203","descriptor_name":"Reproducibility of Results","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015203","descriptor_name":"Reproducibility of Results","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015203","descriptor_name":"Reproducibility of Results","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015894","descriptor_name":"Genome, Human","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D015894","descriptor_name":"Genome, Human","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D015894","descriptor_name":"Genome, Human","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D015894","descriptor_name":"Genome, Human","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D017394","descriptor_name":"RNA, Guide, Kinetoplastida","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D030541","descriptor_name":"Databases, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D030541","descriptor_name":"Databases, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D030541","descriptor_name":"Databases, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D030541","descriptor_name":"Databases, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":4,"locations":[{"id":"doi:10.1093/nar/gkaa848","is_oa":true,"landing_page_url":"https://doi.org/10.1093/nar/gkaa848","pdf_url":"https://academic.oup.com/nar/article-pdf/49/D1/D871/35364779/gkaa848.pdf","source":{"id":"https://openalex.org/S134668137","display_name":"Nucleic Acids Research","issn_l":"0305-1048","issn":["0305-1048","1362-4954","1362-4962"],"is_oa":true,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic Acids Research","raw_type":"journal-article"},{"id":"pmid:33051688","is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/33051688","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic acids research","raw_type":null},{"id":"pmh:oai:europepmc.org:6699092","is_oa":false,"landing_page_url":"http://europepmc.org/pmc/articles/PMC7779039","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Text"},{"id":"pmh:oai:pubmedcentral.nih.gov:7779039","is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/7779039","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Nucleic Acids Res","raw_type":"Text"}],"best_oa_location":{"id":"doi:10.1093/nar/gkaa848","is_oa":true,"landing_page_url":"https://doi.org/10.1093/nar/gkaa848","pdf_url":"https://academic.oup.com/nar/article-pdf/49/D1/D871/35364779/gkaa848.pdf","source":{"id":"https://openalex.org/S134668137","display_name":"Nucleic Acids Research","issn_l":"0305-1048","issn":["0305-1048","1362-4954","1362-4962"],"is_oa":true,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic Acids Research","raw_type":"journal-article"},"sustainable_development_goals":[],"awards":[{"id":"https://openalex.org/G1140788319","display_name":null,"funder_award_id":"Fellowship","funder_id":"https://openalex.org/F4320333063","funder_display_name":"Stanford Bio-X"}],"funders":[{"id":"https://openalex.org/F4320313762","display_name":"Frederick National Laboratory for Cancer Research","ror":"https://ror.org/03v6m3209"},{"id":"https://openalex.org/F4320333063","display_name":"Stanford Bio-X","ror":"https://ror.org/00f54p054"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W3092284562.pdf","grobid_xml":"https://content.openalex.org/works/W3092284562.grobid-xml"},"referenced_works_count":38,"referenced_works":["https://openalex.org/W1869792624","https://openalex.org/W1900589173","https://openalex.org/W1934704664","https://openalex.org/W1968342623","https://openalex.org/W1971191097","https://openalex.org/W1990149668","https://openalex.org/W2003082286","https://openalex.org/W2003171404","https://openalex.org/W2006373538","https://openalex.org/W2031211461","https://openalex.org/W2064815984","https://openalex.org/W2108234281","https://openalex.org/W2110417468","https://openalex.org/W2114245320","https://openalex.org/W2117924928","https://openalex.org/W2128016314","https://openalex.org/W2133836136","https://openalex.org/W2136145671","https://openalex.org/W2157107905","https://openalex.org/W2170818583","https://openalex.org/W2172149993","https://openalex.org/W2180291937","https://openalex.org/W2180658822","https://openalex.org/W2252568502","https://openalex.org/W2461013690","https://openalex.org/W2533641274","https://openalex.org/W2584033231","https://openalex.org/W2584973635","https://openalex.org/W2588613595","https://openalex.org/W2593182764","https://openalex.org/W2763664029","https://openalex.org/W2899807648","https://openalex.org/W2902555008","https://openalex.org/W2945567049","https://openalex.org/W2949998060","https://openalex.org/W2973312556","https://openalex.org/W3000887181","https://openalex.org/W4255738661"],"related_works":["https://openalex.org/W2086525401","https://openalex.org/W2006735166","https://openalex.org/W2079327011","https://openalex.org/W2053325634","https://openalex.org/W3178279768","https://openalex.org/W193851735","https://openalex.org/W2888097224","https://openalex.org/W2773861473","https://openalex.org/W1969895229","https://openalex.org/W2108497208"],"abstract_inverted_index":{"With":[0],"the":[1,51],"technology's":[2],"accessibility":[3],"and":[4,17,53,67,96,148,170,183],"ease":[5],"of":[6,24,39,55,85,110,130,145,161,173],"use,":[7],"CRISPR":[8,28],"has":[9],"been":[10,119],"employed":[11],"widely":[12],"in":[13,35,50,94],"many":[14],"different":[15,164],"organisms":[16],"experimental":[18],"settings.":[19],"As":[20],"a":[21,37,83],"result,":[22],"thousands":[23],"publications":[25],"have":[26,118],"used":[27],"to":[29,48],"make":[30],"specific":[31],"genetic":[32],"perturbations,":[33],"establishing":[34],"itself":[36],"resource":[38],"validated":[40,87,121,150],"guide":[41,57,88,106,151],"RNA":[42,89,107,152],"sequences.":[43],"While":[44],"numerous":[45],"computational":[46],"tools":[47],"assist":[49],"design":[52],"identification":[54],"candidate":[56,105],"RNAs":[58],"exist,":[59],"these":[60],"are":[61],"still":[62],"just":[63],"at":[64],"best":[65],"predictions":[66],"generally,":[68],"researchers":[69],"inevitably":[70],"will":[71,140],"test":[72],"multiple":[73],"sequences":[74,90,116,153,162],"for":[75,91,142,154],"functional":[76],"activity.":[77],"Here,":[78],"we":[79],"present":[80],"dbGuide":[81],"(https://sgrnascorer.cancer.gov/dbguide),":[82],"database":[84,99],"functionally":[86,120],"CRISPR/Cas9-based":[92,155],"knockout":[93,156],"human":[95],"mouse.":[97],"Our":[98],"not":[100],"only":[101],"contains":[102],"computationally":[103],"determined":[104],"sequences,":[108],"but":[109],"even":[111],"greater":[112],"value,":[113],"over":[114,133],"4000":[115],"which":[117],"either":[122],"through":[123],"direct":[124],"amplicon":[125],"sequencing":[126],"or":[127],"manual":[128],"curation":[129],"literature":[131],"from":[132,163],"1000":[134],"publications.":[135],"Finally,":[136],"our":[137],"established":[138],"framework":[139],"allow":[141],"continual":[143],"addition":[144],"newly":[146],"published":[147],"experimentally":[149],"as":[157,159,177],"well":[158],"incorporation":[160],"gene":[165,180],"editing":[166],"systems,":[167],"additional":[168],"species":[169],"other":[171],"types":[172],"site-specific":[174],"functionalities":[175],"such":[176],"base":[178],"editing,":[179],"activation,":[181],"repression":[182],"epigenetic":[184],"modification.":[185]},"counts_by_year":[{"year":2026,"cited_by_count":2},{"year":2025,"cited_by_count":9},{"year":2024,"cited_by_count":4},{"year":2023,"cited_by_count":9},{"year":2022,"cited_by_count":4},{"year":2021,"cited_by_count":6}],"updated_date":"2026-03-27T05:58:40.876381","created_date":"2025-10-10T00:00:00"}