{"id":"https://openalex.org/W2120724457","doi":"https://doi.org/10.1093/database/bar040","title":"GETPrime: a gene- or transcript-specific primer database for quantitative real-time PCR","display_name":"GETPrime: a gene- or transcript-specific primer database for quantitative real-time PCR","publication_year":2011,"publication_date":"2011-01-01","ids":{"openalex":"https://openalex.org/W2120724457","doi":"https://doi.org/10.1093/database/bar040","mag":"2120724457","pmid":"https://pubmed.ncbi.nlm.nih.gov/21917859"},"language":"en","primary_location":{"id":"doi:10.1093/database/bar040","is_oa":true,"landing_page_url":"https://doi.org/10.1093/database/bar040","pdf_url":"https://academic.oup.com/database/article-pdf/doi/10.1093/database/bar040/14094888/bar040.pdf","source":{"id":"https://openalex.org/S4210201630","display_name":"Database","issn_l":"1758-0463","issn":["1758-0463"],"is_oa":true,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310311647","host_organization_name":"University of Oxford","host_organization_lineage":["https://openalex.org/P4310311647"],"host_organization_lineage_names":["University of Oxford"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Database","raw_type":"journal-article"},"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://academic.oup.com/database/article-pdf/doi/10.1093/database/bar040/14094888/bar040.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5027981706","display_name":"Carine Gubelmann","orcid":null},"institutions":[{"id":"https://openalex.org/I12708293","display_name":"SIB Swiss Institute of Bioinformatics","ror":"https://ror.org/002n09z45","country_code":"CH","type":"nonprofit","lineage":["https://openalex.org/I12708293"]},{"id":"https://openalex.org/I5124864","display_name":"\u00c9cole Polytechnique F\u00e9d\u00e9rale de Lausanne","ror":"https://ror.org/02s376052","country_code":"CH","type":"education","lineage":["https://openalex.org/I2799323385","https://openalex.org/I5124864"]}],"countries":["CH"],"is_corresponding":false,"raw_author_name":"Carine Gubelmann","raw_affiliation_strings":["Institute of Bio-engineering, School of Life Sciences, Laboratory of Systems Biology and Genetics, 2Institute of Bio-engineering, School of Life Sciences, Bioinformatics and Biostatistics Core Facility, Ecole Polytechnique F\u00e9d\u00e9rale de Lausanne (EPFL), 3School of Life Sciences, Swiss Institute of Bioinformatics, Station 15, 1015 Lausanne, Switzerland and 4Genohm SA, PSE-C site EPFL, 1015 Lausanne, Switzerland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Bio-engineering, School of Life Sciences, Laboratory of Systems Biology and Genetics, 2Institute of Bio-engineering, School of Life Sciences, Bioinformatics and Biostatistics Core Facility, Ecole Polytechnique F\u00e9d\u00e9rale de Lausanne (EPFL), 3School of Life Sciences, Swiss Institute of Bioinformatics, Station 15, 1015 Lausanne, Switzerland and 4Genohm SA, PSE-C site EPFL, 1015 Lausanne, Switzerland","institution_ids":["https://openalex.org/I5124864","https://openalex.org/I12708293"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5046650365","display_name":"Alexandre Gattiker","orcid":null},"institutions":[{"id":"https://openalex.org/I12708293","display_name":"SIB Swiss Institute of Bioinformatics","ror":"https://ror.org/002n09z45","country_code":"CH","type":"nonprofit","lineage":["https://openalex.org/I12708293"]},{"id":"https://openalex.org/I5124864","display_name":"\u00c9cole Polytechnique F\u00e9d\u00e9rale de Lausanne","ror":"https://ror.org/02s376052","country_code":"CH","type":"education","lineage":["https://openalex.org/I2799323385","https://openalex.org/I5124864"]}],"countries":["CH"],"is_corresponding":false,"raw_author_name":"Alexandre Gattiker","raw_affiliation_strings":["Institute of Bio-engineering, School of Life Sciences, Laboratory of Systems Biology and Genetics, 2Institute of Bio-engineering, School of Life Sciences, Bioinformatics and Biostatistics Core Facility, Ecole Polytechnique F\u00e9d\u00e9rale de Lausanne (EPFL), 3School of Life Sciences, Swiss Institute of Bioinformatics, Station 15, 1015 Lausanne, Switzerland and 4Genohm SA, PSE-C site EPFL, 1015 Lausanne, Switzerland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Bio-engineering, School of Life Sciences, Laboratory of Systems Biology and Genetics, 2Institute of Bio-engineering, School of Life Sciences, Bioinformatics and Biostatistics Core Facility, Ecole Polytechnique F\u00e9d\u00e9rale de Lausanne (EPFL), 3School of Life Sciences, Swiss Institute of Bioinformatics, Station 15, 1015 Lausanne, Switzerland and 4Genohm SA, PSE-C site EPFL, 1015 Lausanne, Switzerland","institution_ids":["https://openalex.org/I5124864","https://openalex.org/I12708293"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5074142338","display_name":"Andreas Massouras","orcid":null},"institutions":[{"id":"https://openalex.org/I12708293","display_name":"SIB Swiss Institute of Bioinformatics","ror":"https://ror.org/002n09z45","country_code":"CH","type":"nonprofit","lineage":["https://openalex.org/I12708293"]},{"id":"https://openalex.org/I5124864","display_name":"\u00c9cole Polytechnique F\u00e9d\u00e9rale de Lausanne","ror":"https://ror.org/02s376052","country_code":"CH","type":"education","lineage":["https://openalex.org/I2799323385","https://openalex.org/I5124864"]}],"countries":["CH"],"is_corresponding":false,"raw_author_name":"Andreas Massouras","raw_affiliation_strings":["Institute of Bio-engineering, School of Life Sciences, Laboratory of Systems Biology and Genetics, 2Institute of Bio-engineering, School of Life Sciences, Bioinformatics and Biostatistics Core Facility, Ecole Polytechnique F\u00e9d\u00e9rale de Lausanne (EPFL), 3School of Life Sciences, Swiss Institute of Bioinformatics, Station 15, 1015 Lausanne, Switzerland and 4Genohm SA, PSE-C site EPFL, 1015 Lausanne, Switzerland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Bio-engineering, School of Life Sciences, Laboratory of Systems Biology and Genetics, 2Institute of Bio-engineering, School of Life Sciences, Bioinformatics and Biostatistics Core Facility, Ecole Polytechnique F\u00e9d\u00e9rale de Lausanne (EPFL), 3School of Life Sciences, Swiss Institute of Bioinformatics, Station 15, 1015 Lausanne, Switzerland and 4Genohm SA, PSE-C site EPFL, 1015 Lausanne, Switzerland","institution_ids":["https://openalex.org/I5124864","https://openalex.org/I12708293"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5088807975","display_name":"Korneel Hens","orcid":"https://orcid.org/0000-0002-0362-7007"},"institutions":[{"id":"https://openalex.org/I12708293","display_name":"SIB Swiss Institute of Bioinformatics","ror":"https://ror.org/002n09z45","country_code":"CH","type":"nonprofit","lineage":["https://openalex.org/I12708293"]},{"id":"https://openalex.org/I5124864","display_name":"\u00c9cole Polytechnique F\u00e9d\u00e9rale de Lausanne","ror":"https://ror.org/02s376052","country_code":"CH","type":"education","lineage":["https://openalex.org/I2799323385","https://openalex.org/I5124864"]}],"countries":["CH"],"is_corresponding":false,"raw_author_name":"Korneel Hens","raw_affiliation_strings":["Institute of Bio-engineering, School of Life Sciences, Laboratory of Systems Biology and Genetics, 2Institute of Bio-engineering, School of Life Sciences, Bioinformatics and Biostatistics Core Facility, Ecole Polytechnique F\u00e9d\u00e9rale de Lausanne (EPFL), 3School of Life Sciences, Swiss Institute of Bioinformatics, Station 15, 1015 Lausanne, Switzerland and 4Genohm SA, PSE-C site EPFL, 1015 Lausanne, Switzerland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Bio-engineering, School of Life Sciences, Laboratory of Systems Biology and Genetics, 2Institute of Bio-engineering, School of Life Sciences, Bioinformatics and Biostatistics Core Facility, Ecole Polytechnique F\u00e9d\u00e9rale de Lausanne (EPFL), 3School of Life Sciences, Swiss Institute of Bioinformatics, Station 15, 1015 Lausanne, Switzerland and 4Genohm SA, PSE-C site EPFL, 1015 Lausanne, Switzerland","institution_ids":["https://openalex.org/I5124864","https://openalex.org/I12708293"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5087373839","display_name":"Fabrice David","orcid":"https://orcid.org/0000-0001-8539-865X"},"institutions":[{"id":"https://openalex.org/I12708293","display_name":"SIB Swiss Institute of Bioinformatics","ror":"https://ror.org/002n09z45","country_code":"CH","type":"nonprofit","lineage":["https://openalex.org/I12708293"]},{"id":"https://openalex.org/I5124864","display_name":"\u00c9cole Polytechnique F\u00e9d\u00e9rale de Lausanne","ror":"https://ror.org/02s376052","country_code":"CH","type":"education","lineage":["https://openalex.org/I2799323385","https://openalex.org/I5124864"]}],"countries":["CH"],"is_corresponding":false,"raw_author_name":"Fabrice David","raw_affiliation_strings":["Institute of Bio-engineering, School of Life Sciences, Laboratory of Systems Biology and Genetics, 2Institute of Bio-engineering, School of Life Sciences, Bioinformatics and Biostatistics Core Facility, Ecole Polytechnique F\u00e9d\u00e9rale de Lausanne (EPFL), 3School of Life Sciences, Swiss Institute of Bioinformatics, Station 15, 1015 Lausanne, Switzerland and 4Genohm SA, PSE-C site EPFL, 1015 Lausanne, Switzerland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Bio-engineering, School of Life Sciences, Laboratory of Systems Biology and Genetics, 2Institute of Bio-engineering, School of Life Sciences, Bioinformatics and Biostatistics Core Facility, Ecole Polytechnique F\u00e9d\u00e9rale de Lausanne (EPFL), 3School of Life Sciences, Swiss Institute of Bioinformatics, Station 15, 1015 Lausanne, Switzerland and 4Genohm SA, PSE-C site EPFL, 1015 Lausanne, Switzerland","institution_ids":["https://openalex.org/I5124864","https://openalex.org/I12708293"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5068232532","display_name":"Frederik Decouttere","orcid":null},"institutions":[{"id":"https://openalex.org/I12708293","display_name":"SIB Swiss Institute of Bioinformatics","ror":"https://ror.org/002n09z45","country_code":"CH","type":"nonprofit","lineage":["https://openalex.org/I12708293"]},{"id":"https://openalex.org/I5124864","display_name":"\u00c9cole Polytechnique F\u00e9d\u00e9rale de Lausanne","ror":"https://ror.org/02s376052","country_code":"CH","type":"education","lineage":["https://openalex.org/I2799323385","https://openalex.org/I5124864"]}],"countries":["CH"],"is_corresponding":false,"raw_author_name":"Frederik Decouttere","raw_affiliation_strings":["Institute of Bio-engineering, School of Life Sciences, Laboratory of Systems Biology and Genetics, 2Institute of Bio-engineering, School of Life Sciences, Bioinformatics and Biostatistics Core Facility, Ecole Polytechnique F\u00e9d\u00e9rale de Lausanne (EPFL), 3School of Life Sciences, Swiss Institute of Bioinformatics, Station 15, 1015 Lausanne, Switzerland and 4Genohm SA, PSE-C site EPFL, 1015 Lausanne, Switzerland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Bio-engineering, School of Life Sciences, Laboratory of Systems Biology and Genetics, 2Institute of Bio-engineering, School of Life Sciences, Bioinformatics and Biostatistics Core Facility, Ecole Polytechnique F\u00e9d\u00e9rale de Lausanne (EPFL), 3School of Life Sciences, Swiss Institute of Bioinformatics, Station 15, 1015 Lausanne, Switzerland and 4Genohm SA, PSE-C site EPFL, 1015 Lausanne, Switzerland","institution_ids":["https://openalex.org/I5124864","https://openalex.org/I12708293"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5008872013","display_name":"Jacques Rougemont","orcid":"https://orcid.org/0000-0002-0955-606X"},"institutions":[{"id":"https://openalex.org/I12708293","display_name":"SIB Swiss Institute of Bioinformatics","ror":"https://ror.org/002n09z45","country_code":"CH","type":"nonprofit","lineage":["https://openalex.org/I12708293"]},{"id":"https://openalex.org/I5124864","display_name":"\u00c9cole Polytechnique F\u00e9d\u00e9rale de Lausanne","ror":"https://ror.org/02s376052","country_code":"CH","type":"education","lineage":["https://openalex.org/I2799323385","https://openalex.org/I5124864"]}],"countries":["CH"],"is_corresponding":false,"raw_author_name":"Jacques Rougemont","raw_affiliation_strings":["Institute of Bio-engineering, School of Life Sciences, Laboratory of Systems Biology and Genetics, 2Institute of Bio-engineering, School of Life Sciences, Bioinformatics and Biostatistics Core Facility, Ecole Polytechnique F\u00e9d\u00e9rale de Lausanne (EPFL), 3School of Life Sciences, Swiss Institute of Bioinformatics, Station 15, 1015 Lausanne, Switzerland and 4Genohm SA, PSE-C site EPFL, 1015 Lausanne, Switzerland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Bio-engineering, School of Life Sciences, Laboratory of Systems Biology and Genetics, 2Institute of Bio-engineering, School of Life Sciences, Bioinformatics and Biostatistics Core Facility, Ecole Polytechnique F\u00e9d\u00e9rale de Lausanne (EPFL), 3School of Life Sciences, Swiss Institute of Bioinformatics, Station 15, 1015 Lausanne, Switzerland and 4Genohm SA, PSE-C site EPFL, 1015 Lausanne, Switzerland","institution_ids":["https://openalex.org/I5124864","https://openalex.org/I12708293"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5060679000","display_name":"Bart Deplancke","orcid":"https://orcid.org/0000-0001-9935-843X"},"institutions":[{"id":"https://openalex.org/I12708293","display_name":"SIB Swiss Institute of Bioinformatics","ror":"https://ror.org/002n09z45","country_code":"CH","type":"nonprofit","lineage":["https://openalex.org/I12708293"]},{"id":"https://openalex.org/I5124864","display_name":"\u00c9cole Polytechnique F\u00e9d\u00e9rale de Lausanne","ror":"https://ror.org/02s376052","country_code":"CH","type":"education","lineage":["https://openalex.org/I2799323385","https://openalex.org/I5124864"]}],"countries":["CH"],"is_corresponding":false,"raw_author_name":"Bart Deplancke","raw_affiliation_strings":["Institute of Bio-engineering, School of Life Sciences, Laboratory of Systems Biology and Genetics, 2Institute of Bio-engineering, School of Life Sciences, Bioinformatics and Biostatistics Core Facility, Ecole Polytechnique F\u00e9d\u00e9rale de Lausanne (EPFL), 3School of Life Sciences, Swiss Institute of Bioinformatics, Station 15, 1015 Lausanne, Switzerland and 4Genohm SA, PSE-C site EPFL, 1015 Lausanne, Switzerland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Bio-engineering, School of Life Sciences, Laboratory of Systems Biology and Genetics, 2Institute of Bio-engineering, School of Life Sciences, Bioinformatics and Biostatistics Core Facility, Ecole Polytechnique F\u00e9d\u00e9rale de Lausanne (EPFL), 3School of Life Sciences, Swiss Institute of Bioinformatics, Station 15, 1015 Lausanne, Switzerland and 4Genohm SA, PSE-C site EPFL, 1015 Lausanne, Switzerland","institution_ids":["https://openalex.org/I5124864","https://openalex.org/I12708293"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":8,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":1415,"currency":"GBP","value_usd":1735},"apc_paid":{"value":1415,"currency":"GBP","value_usd":1735},"fwci":2.2621,"has_fulltext":false,"cited_by_count":48,"citation_normalized_percentile":{"value":0.88305496,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":99},"biblio":{"volume":"2011","issue":null,"first_page":"bar040","last_page":"bar040"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.9998999834060669,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.9998999834060669,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10604","display_name":"RNA Research and Splicing","score":0.9997000098228455,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/splice","display_name":"splice","score":0.7669846415519714},{"id":"https://openalex.org/keywords/primer","display_name":"Primer (cosmetics)","score":0.6914118528366089},{"id":"https://openalex.org/keywords/computational-biology","display_name":"Computational biology","score":0.6143198609352112},{"id":"https://openalex.org/keywords/gene","display_name":"Gene","score":0.5597044229507446},{"id":"https://openalex.org/keywords/biology","display_name":"Biology","score":0.5279718041419983},{"id":"https://openalex.org/keywords/alternative-splicing","display_name":"Alternative splicing","score":0.49726203083992004},{"id":"https://openalex.org/keywords/genetics","display_name":"Genetics","score":0.4944610595703125},{"id":"https://openalex.org/keywords/rna-splicing","display_name":"RNA splicing","score":0.4131268262863159},{"id":"https://openalex.org/keywords/context","display_name":"Context (archaeology)","score":0.41066834330558777},{"id":"https://openalex.org/keywords/database","display_name":"Database","score":0.34222710132598877},{"id":"https://openalex.org/keywords/computer-science","display_name":"Computer science","score":0.30384719371795654},{"id":"https://openalex.org/keywords/rna","display_name":"RNA","score":0.1278878152370453},{"id":"https://openalex.org/keywords/messenger-rna","display_name":"Messenger RNA","score":0.11133259534835815}],"concepts":[{"id":"https://openalex.org/C2780989783","wikidata":"https://www.wikidata.org/wiki/Q7578557","display_name":"splice","level":3,"score":0.7669846415519714},{"id":"https://openalex.org/C2777563447","wikidata":"https://www.wikidata.org/wiki/Q7243397","display_name":"Primer (cosmetics)","level":2,"score":0.6914118528366089},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.6143198609352112},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.5597044229507446},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5279718041419983},{"id":"https://openalex.org/C194583182","wikidata":"https://www.wikidata.org/wiki/Q21114084","display_name":"Alternative splicing","level":4,"score":0.49726203083992004},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.4944610595703125},{"id":"https://openalex.org/C54458228","wikidata":"https://www.wikidata.org/wiki/Q237218","display_name":"RNA splicing","level":4,"score":0.4131268262863159},{"id":"https://openalex.org/C2779343474","wikidata":"https://www.wikidata.org/wiki/Q3109175","display_name":"Context (archaeology)","level":2,"score":0.41066834330558777},{"id":"https://openalex.org/C77088390","wikidata":"https://www.wikidata.org/wiki/Q8513","display_name":"Database","level":1,"score":0.34222710132598877},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.30384719371795654},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.1278878152370453},{"id":"https://openalex.org/C105580179","wikidata":"https://www.wikidata.org/wiki/Q188928","display_name":"Messenger RNA","level":3,"score":0.11133259534835815},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.0},{"id":"https://openalex.org/C151730666","wikidata":"https://www.wikidata.org/wiki/Q7205","display_name":"Paleontology","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003628","descriptor_name":"Database Management Systems","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D003628","descriptor_name":"Database Management Systems","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D003628","descriptor_name":"Database Management Systems","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015203","descriptor_name":"Reproducibility of Results","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015203","descriptor_name":"Reproducibility of Results","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015203","descriptor_name":"Reproducibility of Results","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016475","descriptor_name":"3T3 Cells","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016475","descriptor_name":"3T3 Cells","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016475","descriptor_name":"3T3 Cells","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017931","descriptor_name":"DNA Primers","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017931","descriptor_name":"DNA Primers","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017931","descriptor_name":"DNA Primers","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D020869","descriptor_name":"Gene Expression Profiling","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D020869","descriptor_name":"Gene Expression Profiling","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D020869","descriptor_name":"Gene Expression Profiling","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D023281","descriptor_name":"Genomics","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D023281","descriptor_name":"Genomics","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D023281","descriptor_name":"Genomics","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D030541","descriptor_name":"Databases, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D030541","descriptor_name":"Databases, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D030541","descriptor_name":"Databases, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D060888","descriptor_name":"Real-Time Polymerase Chain Reaction","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D060888","descriptor_name":"Real-Time Polymerase Chain Reaction","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D060888","descriptor_name":"Real-Time Polymerase Chain Reaction","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":5,"locations":[{"id":"doi:10.1093/database/bar040","is_oa":true,"landing_page_url":"https://doi.org/10.1093/database/bar040","pdf_url":"https://academic.oup.com/database/article-pdf/doi/10.1093/database/bar040/14094888/bar040.pdf","source":{"id":"https://openalex.org/S4210201630","display_name":"Database","issn_l":"1758-0463","issn":["1758-0463"],"is_oa":true,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310311647","host_organization_name":"University of Oxford","host_organization_lineage":["https://openalex.org/P4310311647"],"host_organization_lineage_names":["University of Oxford"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Database","raw_type":"journal-article"},{"id":"pmid:21917859","is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/21917859","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Database : the journal of biological databases and curation","raw_type":null},{"id":"pmh:oai:europepmc.org:2178213","is_oa":false,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3173022","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Text"},{"id":"pmh:oai:infoscience.epfl.ch:178513","is_oa":true,"landing_page_url":"http://infoscience.epfl.ch/record/178513","pdf_url":null,"source":{"id":"https://openalex.org/S4306400487","display_name":"Infoscience (Ecole Polytechnique F\u00e9d\u00e9rale de Lausanne)","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Text"},{"id":"pmh:oai:ora.ox.ac.uk:uuid:6931ff49-6df3-4eb1-9e86-5a312bcd31b9","is_oa":false,"landing_page_url":"https://ora.ox.ac.uk/objects/uuid:6931ff49-6df3-4eb1-9e86-5a312bcd31b9","pdf_url":null,"source":{"id":"https://openalex.org/S4306402636","display_name":"Oxford University Research Archive (ORA) (University of Oxford)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I40120149","host_organization_name":"University of Oxford","host_organization_lineage":["https://openalex.org/I40120149"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Symplectic Elements at Oxford","raw_type":"Journal article"}],"best_oa_location":{"id":"doi:10.1093/database/bar040","is_oa":true,"landing_page_url":"https://doi.org/10.1093/database/bar040","pdf_url":"https://academic.oup.com/database/article-pdf/doi/10.1093/database/bar040/14094888/bar040.pdf","source":{"id":"https://openalex.org/S4210201630","display_name":"Database","issn_l":"1758-0463","issn":["1758-0463"],"is_oa":true,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310311647","host_organization_name":"University of Oxford","host_organization_lineage":["https://openalex.org/P4310311647"],"host_organization_lineage_names":["University of Oxford"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Database","raw_type":"journal-article"},"sustainable_development_goals":[],"awards":[{"id":"https://openalex.org/G4274786188","display_name":null,"funder_award_id":"090309/Z/09/Z","funder_id":"https://openalex.org/F4320311904","funder_display_name":"Wellcome Trust"}],"funders":[{"id":"https://openalex.org/F4320311904","display_name":"Wellcome Trust","ror":"https://ror.org/029chgv08"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":38,"referenced_works":["https://openalex.org/W1499500424","https://openalex.org/W1577577364","https://openalex.org/W1749816123","https://openalex.org/W1981222811","https://openalex.org/W2028799613","https://openalex.org/W2040481343","https://openalex.org/W2049284870","https://openalex.org/W2055043387","https://openalex.org/W2071692398","https://openalex.org/W2080152736","https://openalex.org/W2086122679","https://openalex.org/W2092288031","https://openalex.org/W2096940705","https://openalex.org/W2097117811","https://openalex.org/W2098412979","https://openalex.org/W2101039585","https://openalex.org/W2101378463","https://openalex.org/W2103740237","https://openalex.org/W2118930999","https://openalex.org/W2125882871","https://openalex.org/W2128862717","https://openalex.org/W2131463690","https://openalex.org/W2131820599","https://openalex.org/W2132069487","https://openalex.org/W2133976478","https://openalex.org/W2135995702","https://openalex.org/W2138581811","https://openalex.org/W2142966824","https://openalex.org/W2143174447","https://openalex.org/W2150769574","https://openalex.org/W2153489623","https://openalex.org/W2155592392","https://openalex.org/W2155804510","https://openalex.org/W2156281856","https://openalex.org/W2164333136","https://openalex.org/W2167155938","https://openalex.org/W2167408412","https://openalex.org/W6629874611"],"related_works":["https://openalex.org/W2001319824","https://openalex.org/W2067501355","https://openalex.org/W2008389025","https://openalex.org/W1943031701","https://openalex.org/W2136950317","https://openalex.org/W2321791250","https://openalex.org/W4213427798","https://openalex.org/W2023199111","https://openalex.org/W2564462691","https://openalex.org/W2155793948"],"abstract_inverted_index":{"The":[0],"vast":[1],"majority":[2,132],"of":[3,17,29,32,43,121,133,159,198,200],"genes":[4,195,199],"in":[5,25,85,179],"humans":[6],"and":[7,41,81,107,156,192,205],"other":[8],"organisms":[9],"undergo":[10],"alternative":[11],"splicing,":[12],"yet":[13],"the":[14,30,38,119,131,160,183],"biological":[15],"function":[16],"splice":[18,67,79,124,134,140],"variants":[19,45,80,125],"is":[20,56,206],"still":[21],"very":[22],"poorly":[23],"understood":[24],"large":[26],"part":[27],"because":[28],"lack":[31],"simple":[33],"tools":[34],"that":[35,104],"can":[36],"map":[37],"expression":[39,142],"profiles":[40],"patterns":[42],"these":[44],"with":[46],"high":[47,176],"sensitivity.":[48],"High-throughput":[49],"quantitative":[50],"real-time":[51],"polymerase":[52],"chain":[53],"reaction":[54],"(qPCR)":[55],"an":[57],"ideal":[58],"technique":[59],"to":[60,126,153],"accurately":[61],"quantify":[62],"nucleic":[63],"acid":[64],"sequences":[65],"including":[66],"variants.":[68],"However,":[69],"currently":[70],"available":[71,207],"primer":[72,97,115,144,149,162,190],"design":[73],"programs":[74],"do":[75],"not":[76],"distinguish":[77],"between":[78],"also":[82],"differ":[83],"substantially":[84],"overall":[86],"quality,":[87],"functionality":[88],"or":[89,136,196],"throughput":[90],"mode.":[91],"Here,":[92],"we":[93],"present":[94],"GETPrime,":[95],"a":[96,101],"database":[98,187],"supported":[99],"by":[100],"novel":[102],"platform":[103],"uniquely":[105],"combines":[106],"automates":[108],"several":[109],"features":[110],"critical":[111],"for":[112,194],"optimal":[113],"qPCR":[114],"design.":[116],"These":[117],"include":[118],"consideration":[120],"all":[122],"gene":[123],"enable":[127],"either":[128],"gene-specific":[129],"(covering":[130,138],"variants)":[135],"transcript-specific":[137],"one":[139],"variant)":[141],"profiling,":[143],"specificity":[145,178],"validation,":[146],"automated":[147],"best":[148],"pair":[150],"selection":[151],"according":[152],"strict":[154],"criteria":[155],"graphical":[157],"visualization":[158,193],"latter":[161],"pairs":[163],"within":[164],"their":[165],"genomic":[166],"context.":[167],"GETPrime":[168,186],"primers":[169],"have":[170],"been":[171],"extensively":[172],"validated":[173],"experimentally,":[174],"demonstrating":[175],"transcript":[177],"complex":[180],"samples.":[181],"Thus,":[182],"free-access,":[184],"user-friendly":[185],"allows":[188],"fast":[189],"retrieval":[191],"groups":[197],"most":[201],"common":[202],"model":[203],"organisms,":[204],"at":[208],"http://updepla1srv1.epfl.ch/getprime/.":[209],"Database":[210],"URL:":[211],"http://deplanckelab.epfl.ch.":[212]},"counts_by_year":[{"year":2024,"cited_by_count":2},{"year":2023,"cited_by_count":3},{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":3},{"year":2019,"cited_by_count":1},{"year":2018,"cited_by_count":4},{"year":2017,"cited_by_count":5},{"year":2016,"cited_by_count":4},{"year":2015,"cited_by_count":7},{"year":2014,"cited_by_count":8},{"year":2013,"cited_by_count":5},{"year":2012,"cited_by_count":3}],"updated_date":"2026-06-24T13:16:06.693445","created_date":"2025-10-10T00:00:00"}