{"id":"https://openalex.org/W4362515160","doi":"https://doi.org/10.1093/database/baad016","title":"PurificationDB: database of purification conditions for proteins","display_name":"PurificationDB: database of purification conditions for proteins","publication_year":2023,"publication_date":"2023-01-01","ids":{"openalex":"https://openalex.org/W4362515160","doi":"https://doi.org/10.1093/database/baad016","pmid":"https://pubmed.ncbi.nlm.nih.gov/37010519"},"language":"en","primary_location":{"id":"doi:10.1093/database/baad016","is_oa":true,"landing_page_url":"https://doi.org/10.1093/database/baad016","pdf_url":"https://academic.oup.com/database/article-pdf/doi/10.1093/database/baad016/49731471/baad016.pdf","source":{"id":"https://openalex.org/S4210201630","display_name":"Database","issn_l":"1758-0463","issn":["1758-0463"],"is_oa":true,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310311647","host_organization_name":"University of Oxford","host_organization_lineage":["https://openalex.org/P4310311647"],"host_organization_lineage_names":["University of Oxford"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Database","raw_type":"journal-article"},"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://academic.oup.com/database/article-pdf/doi/10.1093/database/baad016/49731471/baad016.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5063044696","display_name":"Olivia Garland","orcid":"https://orcid.org/0000-0003-1903-6276"},"institutions":[{"id":"https://openalex.org/I141945490","display_name":"University of British Columbia","ror":"https://ror.org/03rmrcq20","country_code":"CA","type":"education","lineage":["https://openalex.org/I141945490"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Olivia Garland","raw_affiliation_strings":["Vancouver Prostate Centre, University of British Columbia , 2660 Oak Street, Vancouver, BC V6H 3Z6, Canada"],"raw_orcid":"https://orcid.org/0000-0003-1903-6276","affiliations":[{"raw_affiliation_string":"Vancouver Prostate Centre, University of British Columbia , 2660 Oak Street, Vancouver, BC V6H 3Z6, Canada","institution_ids":["https://openalex.org/I141945490"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5005139941","display_name":"Mariia Radaeva","orcid":"https://orcid.org/0000-0002-1707-9304"},"institutions":[{"id":"https://openalex.org/I141945490","display_name":"University of British Columbia","ror":"https://ror.org/03rmrcq20","country_code":"CA","type":"education","lineage":["https://openalex.org/I141945490"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Mariia Radaeva","raw_affiliation_strings":["Vancouver Prostate Centre, University of British Columbia , 2660 Oak Street, Vancouver, BC V6H 3Z6, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Vancouver Prostate Centre, University of British Columbia , 2660 Oak Street, Vancouver, BC V6H 3Z6, Canada","institution_ids":["https://openalex.org/I141945490"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5014143640","display_name":"Mohit Pandey","orcid":"https://orcid.org/0000-0002-2562-7155"},"institutions":[{"id":"https://openalex.org/I141945490","display_name":"University of British Columbia","ror":"https://ror.org/03rmrcq20","country_code":"CA","type":"education","lineage":["https://openalex.org/I141945490"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Mohit Pandey","raw_affiliation_strings":["Vancouver Prostate Centre, University of British Columbia , 2660 Oak Street, Vancouver, BC V6H 3Z6, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Vancouver Prostate Centre, University of British Columbia , 2660 Oak Street, Vancouver, BC V6H 3Z6, Canada","institution_ids":["https://openalex.org/I141945490"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5070580886","display_name":"Artem Cherkasov","orcid":"https://orcid.org/0000-0002-1599-1439"},"institutions":[{"id":"https://openalex.org/I141945490","display_name":"University of British Columbia","ror":"https://ror.org/03rmrcq20","country_code":"CA","type":"education","lineage":["https://openalex.org/I141945490"]}],"countries":["CA"],"is_corresponding":true,"raw_author_name":"Artem Cherkasov","raw_affiliation_strings":["Vancouver Prostate Centre, University of British Columbia , 2660 Oak Street, Vancouver, BC V6H 3Z6, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Vancouver Prostate Centre, University of British Columbia , 2660 Oak Street, Vancouver, BC V6H 3Z6, Canada","institution_ids":["https://openalex.org/I141945490"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5090453688","display_name":"Nada Lallous","orcid":"https://orcid.org/0000-0002-8665-8641"},"institutions":[{"id":"https://openalex.org/I141945490","display_name":"University of British Columbia","ror":"https://ror.org/03rmrcq20","country_code":"CA","type":"education","lineage":["https://openalex.org/I141945490"]}],"countries":["CA"],"is_corresponding":true,"raw_author_name":"Nada Lallous","raw_affiliation_strings":["Vancouver Prostate Centre, University of British Columbia , 2660 Oak Street, Vancouver, BC V6H 3Z6, Canada"],"raw_orcid":"https://orcid.org/0000-0002-8665-8641","affiliations":[{"raw_affiliation_string":"Vancouver Prostate Centre, University of British Columbia , 2660 Oak Street, Vancouver, BC V6H 3Z6, Canada","institution_ids":["https://openalex.org/I141945490"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":5,"corresponding_author_ids":["https://openalex.org/A5070580886","https://openalex.org/A5090453688"],"corresponding_institution_ids":["https://openalex.org/I141945490"],"apc_list":{"value":1415,"currency":"GBP","value_usd":1735},"apc_paid":{"value":1415,"currency":"GBP","value_usd":1735},"fwci":0.2907,"has_fulltext":true,"cited_by_count":2,"citation_normalized_percentile":{"value":0.61868256,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":90,"max":95},"biblio":{"volume":"2023","issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.9983000159263611,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.9983000159263611,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.9983000159263611,"subfield":{"id":"https://openalex.org/subfields/2505","display_name":"Materials Chemistry"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.9980999827384949,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/uniprot","display_name":"UniProt","score":0.7250664830207825},{"id":"https://openalex.org/keywords/computer-science","display_name":"Computer science","score":0.687468945980072},{"id":"https://openalex.org/keywords/database","display_name":"Database","score":0.5849881768226624},{"id":"https://openalex.org/keywords/function","display_name":"Function (biology)","score":0.505835771560669},{"id":"https://openalex.org/keywords/column","display_name":"Column (typography)","score":0.4631615877151489},{"id":"https://openalex.org/keywords/information-retrieval","display_name":"Information retrieval","score":0.3365491032600403},{"id":"https://openalex.org/keywords/chemistry","display_name":"Chemistry","score":0.15382999181747437},{"id":"https://openalex.org/keywords/biology","display_name":"Biology","score":0.1409866213798523}],"concepts":[{"id":"https://openalex.org/C202264299","wikidata":"https://www.wikidata.org/wiki/Q905695","display_name":"UniProt","level":3,"score":0.7250664830207825},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.687468945980072},{"id":"https://openalex.org/C77088390","wikidata":"https://www.wikidata.org/wiki/Q8513","display_name":"Database","level":1,"score":0.5849881768226624},{"id":"https://openalex.org/C14036430","wikidata":"https://www.wikidata.org/wiki/Q3736076","display_name":"Function (biology)","level":2,"score":0.505835771560669},{"id":"https://openalex.org/C2780551164","wikidata":"https://www.wikidata.org/wiki/Q2306599","display_name":"Column (typography)","level":3,"score":0.4631615877151489},{"id":"https://openalex.org/C23123220","wikidata":"https://www.wikidata.org/wiki/Q816826","display_name":"Information retrieval","level":1,"score":0.3365491032600403},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.15382999181747437},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.1409866213798523},{"id":"https://openalex.org/C76155785","wikidata":"https://www.wikidata.org/wiki/Q418","display_name":"Telecommunications","level":1,"score":0.0},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.0},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0},{"id":"https://openalex.org/C78458016","wikidata":"https://www.wikidata.org/wiki/Q840400","display_name":"Evolutionary biology","level":1,"score":0.0},{"id":"https://openalex.org/C126042441","wikidata":"https://www.wikidata.org/wiki/Q1324888","display_name":"Frame (networking)","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D011506","descriptor_name":"Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D011506","descriptor_name":"Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D011506","descriptor_name":"Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D030562","descriptor_name":"Databases, Protein","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D030562","descriptor_name":"Databases, Protein","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D030562","descriptor_name":"Databases, Protein","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1093/database/baad016","is_oa":true,"landing_page_url":"https://doi.org/10.1093/database/baad016","pdf_url":"https://academic.oup.com/database/article-pdf/doi/10.1093/database/baad016/49731471/baad016.pdf","source":{"id":"https://openalex.org/S4210201630","display_name":"Database","issn_l":"1758-0463","issn":["1758-0463"],"is_oa":true,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310311647","host_organization_name":"University of Oxford","host_organization_lineage":["https://openalex.org/P4310311647"],"host_organization_lineage_names":["University of Oxford"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Database","raw_type":"journal-article"},{"id":"pmid:37010519","is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/37010519","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Database : the journal of biological databases and curation","raw_type":null},{"id":"pmh:oai:pubmedcentral.nih.gov:10069378","is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/10069378","pdf_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC10069378/pdf/baad016.pdf","source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Database (Oxford)","raw_type":"Text"}],"best_oa_location":{"id":"doi:10.1093/database/baad016","is_oa":true,"landing_page_url":"https://doi.org/10.1093/database/baad016","pdf_url":"https://academic.oup.com/database/article-pdf/doi/10.1093/database/baad016/49731471/baad016.pdf","source":{"id":"https://openalex.org/S4210201630","display_name":"Database","issn_l":"1758-0463","issn":["1758-0463"],"is_oa":true,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310311647","host_organization_name":"University of Oxford","host_organization_lineage":["https://openalex.org/P4310311647"],"host_organization_lineage_names":["University of Oxford"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Database","raw_type":"journal-article"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/17","score":0.47999998927116394,"display_name":"Partnerships for the goals"}],"awards":[{"id":"https://openalex.org/G3211250819","display_name":null,"funder_award_id":"461322","funder_id":"https://openalex.org/F4320334506","funder_display_name":"Canadian Institutes of Health Research"},{"id":"https://openalex.org/G4065275875","display_name":null,"funder_award_id":"OV3-170631 VR3-172639","funder_id":"https://openalex.org/F4320334506","funder_display_name":"Canadian Institutes of Health Research"}],"funders":[{"id":"https://openalex.org/F4320334506","display_name":"Canadian Institutes of Health Research","ror":"https://ror.org/01gavpb45"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":36,"referenced_works":["https://openalex.org/W38520883","https://openalex.org/W158246226","https://openalex.org/W207286245","https://openalex.org/W771021118","https://openalex.org/W1920942228","https://openalex.org/W1988743392","https://openalex.org/W1997480152","https://openalex.org/W2025878809","https://openalex.org/W2033936983","https://openalex.org/W2044499660","https://openalex.org/W2053083611","https://openalex.org/W2098187918","https://openalex.org/W2102461176","https://openalex.org/W2105901925","https://openalex.org/W2130479394","https://openalex.org/W2130670576","https://openalex.org/W2152486318","https://openalex.org/W2152745366","https://openalex.org/W2164932286","https://openalex.org/W2166028267","https://openalex.org/W2169750556","https://openalex.org/W2192074939","https://openalex.org/W2220896177","https://openalex.org/W2293907333","https://openalex.org/W2518958453","https://openalex.org/W2531675153","https://openalex.org/W2552825056","https://openalex.org/W2568007517","https://openalex.org/W2791995609","https://openalex.org/W2809517222","https://openalex.org/W2921798619","https://openalex.org/W3033575957","https://openalex.org/W3112376646","https://openalex.org/W4205392984","https://openalex.org/W4241247794","https://openalex.org/W6622572373"],"related_works":["https://openalex.org/W24553703","https://openalex.org/W4211000692","https://openalex.org/W4212920271","https://openalex.org/W2612240612","https://openalex.org/W4211208250","https://openalex.org/W4211054902","https://openalex.org/W4324018414","https://openalex.org/W2170381940","https://openalex.org/W2472351724","https://openalex.org/W3145366215"],"abstract_inverted_index":{"The":[0,45],"isolation":[1],"of":[2,4,43,48,61,83,97,103,130,183],"proteins":[3,49],"interest":[5],"from":[6,138],"cell":[7],"lysates":[8],"is":[9,21,32],"an":[10,117],"integral":[11],"step":[12],"to":[13,52,99,171,179],"study":[14],"protein":[15,27,63,131,151,160,174],"structure":[16],"and":[17,40,59,105,119,127,165,177,191,196],"function.":[18],"Liquid":[19],"chromatography":[20,71],"a":[22],"technique":[23],"commonly":[24],"used":[25],"for":[26,67,81,193],"purification,":[28],"where":[29],"the":[30,36,62,75,87,139,180],"separation":[31],"performed":[33],"by":[34,150],"exploiting":[35],"differences":[37],"in":[38,86],"physical":[39],"chemical":[41],"characteristics":[42],"proteins.":[44],"complex":[46],"nature":[47],"requires":[50],"researchers":[51],"carefully":[53],"choose":[54,74],"buffers":[55],"that":[56,123,187],"maintain":[57],"stability":[58],"activity":[60],"while":[64],"also":[65,154],"allowing":[66],"appropriate":[68],"interaction":[69],"with":[70,158],"columns.":[72],"To":[73,109],"proper":[76],"buffer,":[77],"biochemists":[78],"often":[79,91],"search":[80],"reports":[82],"successful":[84],"purification":[85,132,175],"literature;":[88],"however,":[89],"they":[90],"encounter":[92],"roadblocks":[93],"such":[94,111],"as":[95],"lack":[96],"accessibility":[98],"journals,":[100],"non-exhaustive":[101],"specification":[102],"components":[104],"unfamiliar":[106],"naming":[107],"conventions.":[108],"overcome":[110],"issues,":[112],"we":[113],"present":[114],"PurificationDB":[115,153,167],"(https://purificationdatabase.herokuapp.com/),":[116],"open-access":[118],"user-friendly":[120],"knowledge":[121],"base":[122],"contains":[124],"4732":[125],"curated":[126],"standardized":[128],"entries":[129],"conditions.":[133],"Buffer":[134],"specifications":[135],"were":[136],"derived":[137],"literature":[140],"using":[141,146],"named-entity":[142],"recognition":[143],"techniques":[144,176],"developed":[145],"common":[147],"nomenclature":[148],"provided":[149],"biochemists.":[152],"incorporates":[155],"information":[156],"associated":[157],"well-known":[159],"databases:":[161],"Protein":[162],"Data":[163],"Bank":[164],"UniProt.":[166],"facilitates":[168],"easy":[169],"access":[170,195],"data":[172,192],"on":[173],"contributes":[178],"growing":[181],"effort":[182],"creating":[184],"open":[185],"resources":[186],"organize":[188],"experimental":[189],"conditions":[190],"improved":[194],"analysis.":[197],"Database":[198],"URL":[199],"https://purificationdatabase.herokuapp.com/.":[200]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":1}],"updated_date":"2026-05-21T06:26:12.895304","created_date":"2025-10-10T00:00:00"}