{"id":"https://openalex.org/W4242653962","doi":"https://doi.org/10.1093/bioinformatics/btx695","title":"Cpf1-Database: web-based genome-wide guide RNA library design for gene knockout screens using CRISPR-Cpf1","display_name":"Cpf1-Database: web-based genome-wide guide RNA library design for gene knockout screens using CRISPR-Cpf1","publication_year":2017,"publication_date":"2017-11-24","ids":{"openalex":"https://openalex.org/W4242653962","doi":"https://doi.org/10.1093/bioinformatics/btx695"},"language":"en","primary_location":{"id":"doi:10.1093/bioinformatics/btx695","is_oa":true,"landing_page_url":"https://doi.org/10.1093/bioinformatics/btx695","pdf_url":"https://academic.oup.com/bioinformatics/article-pdf/34/6/1077/25119179/btx695.pdf","source":{"id":"https://openalex.org/S52395412","display_name":"Bioinformatics","issn_l":"1367-4803","issn":["1367-4803","1367-4811"],"is_oa":false,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Bioinformatics","raw_type":"journal-article"},"type":"article","indexed_in":["crossref","doaj"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://academic.oup.com/bioinformatics/article-pdf/34/6/1077/25119179/btx695.pdf","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5026647323","display_name":"Jeongbin Park","orcid":"https://orcid.org/0000-0002-9064-4912"},"institutions":[{"id":"https://openalex.org/I4575257","display_name":"Hanyang University","ror":"https://ror.org/046865y68","country_code":"KR","type":"education","lineage":["https://openalex.org/I4575257"]}],"countries":["KR"],"is_corresponding":false,"raw_author_name":"Jeongbin Park","raw_affiliation_strings":["Department of Chemistry, Hanyang University, Seoul, South Korea"],"affiliations":[{"raw_affiliation_string":"Department of Chemistry, Hanyang University, Seoul, South Korea","institution_ids":["https://openalex.org/I4575257"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5058387827","display_name":"Sangsu Bae","orcid":"https://orcid.org/0000-0003-3615-8566"},"institutions":[{"id":"https://openalex.org/I4575257","display_name":"Hanyang University","ror":"https://ror.org/046865y68","country_code":"KR","type":"education","lineage":["https://openalex.org/I4575257"]}],"countries":["KR"],"is_corresponding":true,"raw_author_name":"Sangsu Bae","raw_affiliation_strings":["Department of Chemistry, Hanyang University, Seoul, South Korea","Research Institute for Convergence of Basic Sciences, Hanyang University, Seoul, South Korea"],"affiliations":[{"raw_affiliation_string":"Department of Chemistry, Hanyang University, Seoul, South Korea","institution_ids":["https://openalex.org/I4575257"]},{"raw_affiliation_string":"Research Institute for Convergence of Basic Sciences, Hanyang University, Seoul, South Korea","institution_ids":["https://openalex.org/I4575257"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5058387827"],"corresponding_institution_ids":["https://openalex.org/I4575257"],"apc_list":{"value":3618,"currency":"USD","value_usd":3618},"apc_paid":null,"fwci":1.1697,"has_fulltext":true,"cited_by_count":28,"citation_normalized_percentile":{"value":0.78611281,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":99},"biblio":{"volume":"34","issue":"6","first_page":"1077","last_page":"1079"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":0.9984999895095825,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12610","display_name":"RNA regulation and disease","score":0.9947999715805054,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/crispr","display_name":"CRISPR","score":0.7672728896141052},{"id":"https://openalex.org/keywords/guide-rna","display_name":"Guide RNA","score":0.6152431964874268},{"id":"https://openalex.org/keywords/computer-science","display_name":"Computer science","score":0.564984917640686},{"id":"https://openalex.org/keywords/genome-editing","display_name":"Genome editing","score":0.5237695574760437},{"id":"https://openalex.org/keywords/genome","display_name":"Genome","score":0.5196729898452759},{"id":"https://openalex.org/keywords/computational-biology","display_name":"Computational biology","score":0.46262288093566895},{"id":"https://openalex.org/keywords/database","display_name":"Database","score":0.35465216636657715},{"id":"https://openalex.org/keywords/biology","display_name":"Biology","score":0.3240112364292145},{"id":"https://openalex.org/keywords/genetics","display_name":"Genetics","score":0.27821484208106995},{"id":"https://openalex.org/keywords/gene","display_name":"Gene","score":0.25310415029525757}],"concepts":[{"id":"https://openalex.org/C98108389","wikidata":"https://www.wikidata.org/wiki/Q412563","display_name":"CRISPR","level":3,"score":0.7672728896141052},{"id":"https://openalex.org/C97702854","wikidata":"https://www.wikidata.org/wiki/Q4039747","display_name":"Guide RNA","level":5,"score":0.6152431964874268},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.564984917640686},{"id":"https://openalex.org/C144501496","wikidata":"https://www.wikidata.org/wiki/Q5533489","display_name":"Genome editing","level":4,"score":0.5237695574760437},{"id":"https://openalex.org/C141231307","wikidata":"https://www.wikidata.org/wiki/Q7020","display_name":"Genome","level":3,"score":0.5196729898452759},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.46262288093566895},{"id":"https://openalex.org/C77088390","wikidata":"https://www.wikidata.org/wiki/Q8513","display_name":"Database","level":1,"score":0.35465216636657715},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3240112364292145},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.27821484208106995},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.25310415029525757}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1093/bioinformatics/btx695","is_oa":true,"landing_page_url":"https://doi.org/10.1093/bioinformatics/btx695","pdf_url":"https://academic.oup.com/bioinformatics/article-pdf/34/6/1077/25119179/btx695.pdf","source":{"id":"https://openalex.org/S52395412","display_name":"Bioinformatics","issn_l":"1367-4803","issn":["1367-4803","1367-4811"],"is_oa":false,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Bioinformatics","raw_type":"journal-article"}],"best_oa_location":{"id":"doi:10.1093/bioinformatics/btx695","is_oa":true,"landing_page_url":"https://doi.org/10.1093/bioinformatics/btx695","pdf_url":"https://academic.oup.com/bioinformatics/article-pdf/34/6/1077/25119179/btx695.pdf","source":{"id":"https://openalex.org/S52395412","display_name":"Bioinformatics","issn_l":"1367-4803","issn":["1367-4803","1367-4811"],"is_oa":false,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Bioinformatics","raw_type":"journal-article"},"sustainable_development_goals":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W4242653962.pdf","grobid_xml":"https://content.openalex.org/works/W4242653962.grobid-xml"},"referenced_works_count":18,"referenced_works":["https://openalex.org/W1982846895","https://openalex.org/W1982873976","https://openalex.org/W2025412492","https://openalex.org/W2026592619","https://openalex.org/W2039705224","https://openalex.org/W2050480410","https://openalex.org/W2114245320","https://openalex.org/W2115603949","https://openalex.org/W2122563582","https://openalex.org/W2123724865","https://openalex.org/W2131581981","https://openalex.org/W2165869860","https://openalex.org/W2180291937","https://openalex.org/W2290032338","https://openalex.org/W2305742620","https://openalex.org/W2410526873","https://openalex.org/W2465120080","https://openalex.org/W2621786780"],"related_works":["https://openalex.org/W4366822718","https://openalex.org/W3030388918","https://openalex.org/W4200005163","https://openalex.org/W2807464733","https://openalex.org/W4220766053","https://openalex.org/W2603161682","https://openalex.org/W4213248511","https://openalex.org/W3165203721","https://openalex.org/W2667943050","https://openalex.org/W2117924928"],"abstract_inverted_index":{"Abstract":[0],"Summary":[1],"Following":[2],"the":[3,69,89,96,105],"type":[4,8],"II":[5],"CRISPR-Cas9":[6],"system,":[7],"V":[9],"CRISPR-Cpf1":[10],"endonucleases":[11],"have":[12,62],"been":[13],"found":[14],"to":[15,82],"be":[16],"applicable":[17],"for":[18,57,85,114],"genome":[19,90],"editing":[20],"in":[21,24,118],"various":[22],"organisms":[23],"vivo.":[25],"However,":[26],"there":[27],"are":[28],"as":[29],"yet":[30],"no":[31],"web-based":[32],"tools":[33],"capable":[34],"of":[35,66,72,116],"optimally":[36],"selecting":[37],"guide":[38],"RNAs":[39],"(gRNAs)":[40],"among":[41],"all":[42],"possible":[43],"genome-wide":[44,52],"target":[45,73],"sites.":[46,74],"Here,":[47],"we":[48],"present":[49],"Cpf1-Database,":[50],"a":[51,77,99],"gRNA":[53],"library":[54],"design":[55,83,112],"tool":[56],"LbCpf1":[58],"and":[59,103,122],"AsCpf1,":[60],"which":[61],"DNA":[63],"recognition":[64],"sequences":[65],"5\u2032-TTTN-3\u2032":[67],"at":[68,126],"5\u2032":[70],"ends":[71],"Cpf1-Database":[75],"provides":[76],"sophisticated":[78],"but":[79],"simple":[80],"way":[81],"gRNAs":[84,113],"AsCpf1":[86],"nucleases":[87],"on":[88],"scale.":[91],"One":[92],"can":[93,110],"easily":[94,111],"access":[95,125],"data":[97],"using":[98,104],"straightforward":[100],"web":[101],"interface,":[102],"powerful":[106],"collections":[107],"feature":[108],"one":[109],"thousands":[115],"genes":[117],"short":[119],"time.":[120],"Availability":[121],"implementation":[123],"Free":[124],"http://www.rgenome.net/cpf1-database/.":[127]},"counts_by_year":[{"year":2025,"cited_by_count":4},{"year":2024,"cited_by_count":2},{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":5},{"year":2021,"cited_by_count":5},{"year":2020,"cited_by_count":7},{"year":2019,"cited_by_count":3},{"year":2018,"cited_by_count":1}],"updated_date":"2025-11-06T03:46:38.306776","created_date":"2025-10-10T00:00:00"}