{"id":"https://openalex.org/W2329821483","doi":"https://doi.org/10.1093/bioinformatics/btw172","title":"2dSpAn: semiautomated 2-d segmentation, classification and analysis of hippocampal dendritic spine plasticity","display_name":"2dSpAn: semiautomated 2-d segmentation, classification and analysis of hippocampal dendritic spine plasticity","publication_year":2016,"publication_date":"2016-04-01","ids":{"openalex":"https://openalex.org/W2329821483","doi":"https://doi.org/10.1093/bioinformatics/btw172","mag":"2329821483","pmid":"https://pubmed.ncbi.nlm.nih.gov/27153678"},"language":"en","primary_location":{"id":"doi:10.1093/bioinformatics/btw172","is_oa":false,"landing_page_url":"https://doi.org/10.1093/bioinformatics/btw172","pdf_url":null,"source":{"id":"https://openalex.org/S52395412","display_name":"Bioinformatics","issn_l":"1367-4803","issn":["1367-4803","1367-4811"],"is_oa":false,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Bioinformatics","raw_type":"journal-article"},"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5043105133","display_name":"Subhadip Basu","orcid":"https://orcid.org/0000-0003-1780-0461"},"institutions":[{"id":"https://openalex.org/I170979836","display_name":"Jadavpur University","ror":"https://ror.org/02af4h012","country_code":"IN","type":"education","lineage":["https://openalex.org/I170979836"]}],"countries":["IN"],"is_corresponding":true,"raw_author_name":"Subhadip Basu","raw_affiliation_strings":["1 Department of Computer Science and Engineering, Jadavpur University, Kolkata 700032, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1 Department of Computer Science and Engineering, Jadavpur University, Kolkata 700032, India","institution_ids":["https://openalex.org/I170979836"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5021916927","display_name":"Dariusz Plewczy\u0144ski","orcid":"https://orcid.org/0000-0002-3840-7610"},"institutions":[{"id":"https://openalex.org/I4654613","display_name":"University of Warsaw","ror":"https://ror.org/039bjqg32","country_code":"PL","type":"education","lineage":["https://openalex.org/I4654613"]}],"countries":["PL"],"is_corresponding":false,"raw_author_name":"Dariusz Plewczynski","raw_affiliation_strings":["2 Center of New Technologies, University of Warsaw, Warsaw, Poland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"2 Center of New Technologies, University of Warsaw, Warsaw, Poland","institution_ids":["https://openalex.org/I4654613"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101751418","display_name":"Satadal Saha","orcid":"https://orcid.org/0000-0002-6994-0792"},"institutions":[{"id":"https://openalex.org/I98365261","display_name":"Indian Institute of Engineering Science and Technology, Shibpur","ror":"https://ror.org/02ytfzr55","country_code":"IN","type":"education","lineage":["https://openalex.org/I98365261"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Satadal Saha","raw_affiliation_strings":["3 Department of Electronics and Communication Engineering, MCKV Institute of Engineering, Howrah 711204, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"3 Department of Electronics and Communication Engineering, MCKV Institute of Engineering, Howrah 711204, India","institution_ids":["https://openalex.org/I98365261"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5021734302","display_name":"Matylda Roszkowska","orcid":"https://orcid.org/0000-0001-9280-8063"},"institutions":[{"id":"https://openalex.org/I16778253","display_name":"Instytut Biologii Do\u015bwiadczalnej im. Marcelego Nenckiego","ror":"https://ror.org/04waf7p94","country_code":"PL","type":"facility","lineage":["https://openalex.org/I16778253","https://openalex.org/I99542240"]},{"id":"https://openalex.org/I99542240","display_name":"Polish Academy of Sciences","ror":"https://ror.org/01dr6c206","country_code":"PL","type":"government","lineage":["https://openalex.org/I99542240"]}],"countries":["PL"],"is_corresponding":false,"raw_author_name":"Matylda Roszkowska","raw_affiliation_strings":["4 Department of Molecular and Cellular Neurobiology, Nencki Institute of Experimental Biology, Polish Academy of Sciences, Warsaw, Poland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"4 Department of Molecular and Cellular Neurobiology, Nencki Institute of Experimental Biology, Polish Academy of Sciences, Warsaw, Poland","institution_ids":["https://openalex.org/I99542240","https://openalex.org/I16778253"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5028334064","display_name":"Marta Magnowska","orcid":"https://orcid.org/0000-0002-5424-6837"},"institutions":[{"id":"https://openalex.org/I16778253","display_name":"Instytut Biologii Do\u015bwiadczalnej im. Marcelego Nenckiego","ror":"https://ror.org/04waf7p94","country_code":"PL","type":"facility","lineage":["https://openalex.org/I16778253","https://openalex.org/I99542240"]},{"id":"https://openalex.org/I99542240","display_name":"Polish Academy of Sciences","ror":"https://ror.org/01dr6c206","country_code":"PL","type":"government","lineage":["https://openalex.org/I99542240"]}],"countries":["PL"],"is_corresponding":false,"raw_author_name":"Marta Magnowska","raw_affiliation_strings":["4 Department of Molecular and Cellular Neurobiology, Nencki Institute of Experimental Biology, Polish Academy of Sciences, Warsaw, Poland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"4 Department of Molecular and Cellular Neurobiology, Nencki Institute of Experimental Biology, Polish Academy of Sciences, Warsaw, Poland","institution_ids":["https://openalex.org/I99542240","https://openalex.org/I16778253"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5028280199","display_name":"Ewa B\u0105czy\u0144ska","orcid":"https://orcid.org/0000-0003-0858-4816"},"institutions":[{"id":"https://openalex.org/I16778253","display_name":"Instytut Biologii Do\u015bwiadczalnej im. Marcelego Nenckiego","ror":"https://ror.org/04waf7p94","country_code":"PL","type":"facility","lineage":["https://openalex.org/I16778253","https://openalex.org/I99542240"]},{"id":"https://openalex.org/I99542240","display_name":"Polish Academy of Sciences","ror":"https://ror.org/01dr6c206","country_code":"PL","type":"government","lineage":["https://openalex.org/I99542240"]}],"countries":["PL"],"is_corresponding":false,"raw_author_name":"Ewa Baczynska","raw_affiliation_strings":["4 Department of Molecular and Cellular Neurobiology, Nencki Institute of Experimental Biology, Polish Academy of Sciences, Warsaw, Poland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"4 Department of Molecular and Cellular Neurobiology, Nencki Institute of Experimental Biology, Polish Academy of Sciences, Warsaw, Poland","institution_ids":["https://openalex.org/I99542240","https://openalex.org/I16778253"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5042164534","display_name":"Jakub W\u0142odarczyk","orcid":"https://orcid.org/0000-0001-8343-2340"},"institutions":[{"id":"https://openalex.org/I16778253","display_name":"Instytut Biologii Do\u015bwiadczalnej im. Marcelego Nenckiego","ror":"https://ror.org/04waf7p94","country_code":"PL","type":"facility","lineage":["https://openalex.org/I16778253","https://openalex.org/I99542240"]},{"id":"https://openalex.org/I99542240","display_name":"Polish Academy of Sciences","ror":"https://ror.org/01dr6c206","country_code":"PL","type":"government","lineage":["https://openalex.org/I99542240"]}],"countries":["PL"],"is_corresponding":true,"raw_author_name":"Jakub Wlodarczyk","raw_affiliation_strings":["4 Department of Molecular and Cellular Neurobiology, Nencki Institute of Experimental Biology, Polish Academy of Sciences, Warsaw, Poland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"4 Department of Molecular and Cellular Neurobiology, Nencki Institute of Experimental Biology, Polish Academy of Sciences, Warsaw, Poland","institution_ids":["https://openalex.org/I99542240","https://openalex.org/I16778253"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":7,"corresponding_author_ids":["https://openalex.org/A5042164534","https://openalex.org/A5043105133"],"corresponding_institution_ids":["https://openalex.org/I16778253","https://openalex.org/I170979836","https://openalex.org/I99542240"],"apc_list":{"value":3618,"currency":"USD","value_usd":3618},"apc_paid":null,"fwci":2.8618,"has_fulltext":false,"cited_by_count":32,"citation_normalized_percentile":{"value":0.89896923,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":98},"biblio":{"volume":"32","issue":"16","first_page":"2490","last_page":"2498"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10077","display_name":"Neuroscience and Neuropharmacology Research","score":0.9997000098228455,"subfield":{"id":"https://openalex.org/subfields/2804","display_name":"Cellular and Molecular Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10077","display_name":"Neuroscience and Neuropharmacology Research","score":0.9997000098228455,"subfield":{"id":"https://openalex.org/subfields/2804","display_name":"Cellular and Molecular Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12859","display_name":"Cell Image Analysis Techniques","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10608","display_name":"Neurogenesis and neuroplasticity mechanisms","score":0.995199978351593,"subfield":{"id":"https://openalex.org/subfields/2806","display_name":"Developmental Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/dendritic-spine","display_name":"Dendritic spine","score":0.8925024271011353},{"id":"https://openalex.org/keywords/segmentation","display_name":"Segmentation","score":0.766444206237793},{"id":"https://openalex.org/keywords/computer-science","display_name":"Computer science","score":0.6716346144676208},{"id":"https://openalex.org/keywords/spine","display_name":"SPINE (molecular biology)","score":0.574596643447876},{"id":"https://openalex.org/keywords/artificial-intelligence","display_name":"Artificial intelligence","score":0.552449643611908},{"id":"https://openalex.org/keywords/software","display_name":"Software","score":0.5236485004425049},{"id":"https://openalex.org/keywords/hippocampal-formation","display_name":"Hippocampal formation","score":0.47360968589782715},{"id":"https://openalex.org/keywords/filopodia","display_name":"Filopodia","score":0.4415394365787506},{"id":"https://openalex.org/keywords/pattern-recognition","display_name":"Pattern recognition (psychology)","score":0.3826693892478943},{"id":"https://openalex.org/keywords/computer-vision","display_name":"Computer vision","score":0.36320698261260986},{"id":"https://openalex.org/keywords/biology","display_name":"Biology","score":0.26575130224227905},{"id":"https://openalex.org/keywords/neuroscience","display_name":"Neuroscience","score":0.2552337944507599},{"id":"https://openalex.org/keywords/bioinformatics","display_name":"Bioinformatics","score":0.1963512897491455},{"id":"https://openalex.org/keywords/actin","display_name":"Actin","score":0.09699472784996033}],"concepts":[{"id":"https://openalex.org/C174884520","wikidata":"https://www.wikidata.org/wiki/Q902079","display_name":"Dendritic spine","level":3,"score":0.8925024271011353},{"id":"https://openalex.org/C89600930","wikidata":"https://www.wikidata.org/wiki/Q1423946","display_name":"Segmentation","level":2,"score":0.766444206237793},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.6716346144676208},{"id":"https://openalex.org/C205383261","wikidata":"https://www.wikidata.org/wiki/Q7392570","display_name":"SPINE (molecular biology)","level":2,"score":0.574596643447876},{"id":"https://openalex.org/C154945302","wikidata":"https://www.wikidata.org/wiki/Q11660","display_name":"Artificial intelligence","level":1,"score":0.552449643611908},{"id":"https://openalex.org/C2777904410","wikidata":"https://www.wikidata.org/wiki/Q7397","display_name":"Software","level":2,"score":0.5236485004425049},{"id":"https://openalex.org/C148762608","wikidata":"https://www.wikidata.org/wiki/Q3748185","display_name":"Hippocampal formation","level":2,"score":0.47360968589782715},{"id":"https://openalex.org/C90301020","wikidata":"https://www.wikidata.org/wiki/Q14859810","display_name":"Filopodia","level":3,"score":0.4415394365787506},{"id":"https://openalex.org/C153180895","wikidata":"https://www.wikidata.org/wiki/Q7148389","display_name":"Pattern recognition (psychology)","level":2,"score":0.3826693892478943},{"id":"https://openalex.org/C31972630","wikidata":"https://www.wikidata.org/wiki/Q844240","display_name":"Computer vision","level":1,"score":0.36320698261260986},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.26575130224227905},{"id":"https://openalex.org/C169760540","wikidata":"https://www.wikidata.org/wiki/Q207011","display_name":"Neuroscience","level":1,"score":0.2552337944507599},{"id":"https://openalex.org/C60644358","wikidata":"https://www.wikidata.org/wiki/Q128570","display_name":"Bioinformatics","level":1,"score":0.1963512897491455},{"id":"https://openalex.org/C125705527","wikidata":"https://www.wikidata.org/wiki/Q185269","display_name":"Actin","level":2,"score":0.09699472784996033},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.0},{"id":"https://openalex.org/C199360897","wikidata":"https://www.wikidata.org/wiki/Q9143","display_name":"Programming language","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D003712","descriptor_name":"Dendrites","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003712","descriptor_name":"Dendrites","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003712","descriptor_name":"Dendrites","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006624","descriptor_name":"Hippocampus","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D006624","descriptor_name":"Hippocampus","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D006624","descriptor_name":"Hippocampus","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D012984","descriptor_name":"Software","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012984","descriptor_name":"Software","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012984","descriptor_name":"Software","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015203","descriptor_name":"Reproducibility of Results","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015203","descriptor_name":"Reproducibility of Results","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015203","descriptor_name":"Reproducibility of Results","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018613","descriptor_name":"Microscopy, Confocal","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D018613","descriptor_name":"Microscopy, Confocal","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D018613","descriptor_name":"Microscopy, Confocal","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D049229","descriptor_name":"Dendritic Spines","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D049229","descriptor_name":"Dendritic Spines","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D049229","descriptor_name":"Dendritic Spines","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":3,"locations":[{"id":"doi:10.1093/bioinformatics/btw172","is_oa":false,"landing_page_url":"https://doi.org/10.1093/bioinformatics/btw172","pdf_url":null,"source":{"id":"https://openalex.org/S52395412","display_name":"Bioinformatics","issn_l":"1367-4803","issn":["1367-4803","1367-4811"],"is_oa":false,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Bioinformatics","raw_type":"journal-article"},{"id":"pmid:27153678","is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/27153678","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Bioinformatics (Oxford, England)","raw_type":null},{"id":"pmh:oai:open-archive.highwire.org:bioinfo:32/16/2490","is_oa":false,"landing_page_url":"http://bioinformatics.oxfordjournals.org/cgi/content/short/32/16/2490","pdf_url":null,"source":{"id":"https://openalex.org/S4406923041","display_name":"HighWire Press Open Archive","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"TEXT"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Partnerships for the goals","score":0.47999998927116394,"id":"https://metadata.un.org/sdg/17"}],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":38,"referenced_works":["https://openalex.org/W151176851","https://openalex.org/W1016634212","https://openalex.org/W1533227151","https://openalex.org/W1552913606","https://openalex.org/W1587505135","https://openalex.org/W1592074189","https://openalex.org/W1798313689","https://openalex.org/W1859826371","https://openalex.org/W1983553517","https://openalex.org/W1986605182","https://openalex.org/W1999893555","https://openalex.org/W2001528712","https://openalex.org/W2003879033","https://openalex.org/W2007222931","https://openalex.org/W2017613746","https://openalex.org/W2022818193","https://openalex.org/W2033125075","https://openalex.org/W2042965441","https://openalex.org/W2046900303","https://openalex.org/W2061628168","https://openalex.org/W2069537876","https://openalex.org/W2070245441","https://openalex.org/W2077476134","https://openalex.org/W2096579040","https://openalex.org/W2096610613","https://openalex.org/W2113786093","https://openalex.org/W2114538750","https://openalex.org/W2133059825","https://openalex.org/W2138825607","https://openalex.org/W2139392105","https://openalex.org/W2148714108","https://openalex.org/W2149906678","https://openalex.org/W2160308091","https://openalex.org/W2164811687","https://openalex.org/W2167279371","https://openalex.org/W2168530657","https://openalex.org/W4246084279","https://openalex.org/W6680096826"],"related_works":["https://openalex.org/W4200170201","https://openalex.org/W2169505641","https://openalex.org/W1996493542","https://openalex.org/W2054116827","https://openalex.org/W2120160245","https://openalex.org/W3132088562","https://openalex.org/W2136747326","https://openalex.org/W1974330164","https://openalex.org/W2060098116","https://openalex.org/W1989838035"],"abstract_inverted_index":{"MOTIVATION:":[0],"Accurate":[1],"and":[2,32,86,126,139,155,181],"effective":[3],"dendritic":[4,39,101,174],"spine":[5,112],"segmentation":[6,44],"from":[7,55,103],"the":[8,53,56,69,123,133,144,148,160,172,182],"dendrites":[9],"remains":[10],"as":[11],"a":[12,24],"challenge":[13],"for":[14,28,118,192],"current":[15],"neuroimaging":[16],"research":[17],"community.":[18],"In":[19],"this":[20],"article,":[21],"we":[22],"present":[23],"new":[25],"method":[26,45,92,152],"(2dSpAn)":[27],"2-d":[29],"segmentation,":[30],"classification":[31],"analysis":[33,110,117,129],"of":[34,37,71,80,100,111,120,122,147,162,171],"structural/plastic":[35],"changes":[36],"hippocampal":[38,105],"spines.":[40,73],"A":[41],"user":[42],"interactive":[43],"with":[46,130,141],"convolution":[47],"kernels":[48],"is":[49,93,153],"designed":[50],"to":[51,63,68,132,143,157],"segment":[52],"spines":[54,102,164],"dendrites.":[57],"Formal":[58],"morphological":[59,113],"definitions":[60],"are":[61,75,185,203],"presented":[62],"describe":[64,159],"key":[65],"attributes":[66],"related":[67],"shape":[70],"segmented":[72],"Spines":[74],"automatically":[76],"classified":[77],"into":[78],"one":[79],"four":[81],"classes:":[82],"Stubby,":[83],"Filopodia,":[84],"Mushroom":[85],"Spine-head":[87],"Protrusions.":[88],"RESULTS:":[89],"The":[90,150,179],"developed":[91,124,151],"validated":[94],"using":[95],"confocal":[96],"light":[97],"microscopy":[98],"images":[99,170],"dissociated":[104],"cultures":[106],"for:":[107],"(i)":[108],"quantitative":[109],"changes,":[114],"(ii)":[115],"reproducibility":[116],"assessment":[119],"user-independence":[121],"software":[125,180],"(iii)":[127],"accuracy":[128],"respect":[131,142],"manually":[134],"labeled":[135],"ground":[136],"truth":[137],"images,":[138],"also":[140],"available":[145,186,204],"state":[146],"art.":[149],"monitored":[154],"used":[156],"precisely":[158],"morphology":[161],"individual":[163],"in":[165],"real-time":[166],"experiments,":[167],"i.e.":[168],"consequent":[169],"same":[173],"fragment.":[175],"AVAILABILITY":[176],"AND":[177],"IMPLEMENTATION:":[178],"source":[183],"code":[184],"at":[187,205],"https://sites.google.com/site/2dspan/":[188],"under":[189],"open-source":[190],"license":[191],"non-commercial":[193],"use.":[194],"CONTACT:":[195],"subhadip@cse.jdvu.ac.in":[196],"or":[197],"j.wlodarczyk@nencki.gov.pl":[198],"SUPPLEMENTARY":[199],"INFORMATION:":[200],"Supplementary":[201],"data":[202],"Bioinformatics":[206],"online.":[207]},"counts_by_year":[{"year":2025,"cited_by_count":2},{"year":2024,"cited_by_count":2},{"year":2023,"cited_by_count":4},{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":5},{"year":2019,"cited_by_count":3},{"year":2018,"cited_by_count":5},{"year":2017,"cited_by_count":5},{"year":2016,"cited_by_count":4}],"updated_date":"2026-06-20T22:02:38.213706","created_date":"2016-06-24T00:00:00"}