{"id":"https://openalex.org/W2131629152","doi":"https://doi.org/10.1093/bioinformatics/btt672","title":"FastPros: screening of reaction knockout strategies for metabolic engineering","display_name":"FastPros: screening of reaction knockout strategies for metabolic engineering","publication_year":2013,"publication_date":"2013-11-19","ids":{"openalex":"https://openalex.org/W2131629152","doi":"https://doi.org/10.1093/bioinformatics/btt672","mag":"2131629152","pmid":"https://pubmed.ncbi.nlm.nih.gov/24257186"},"language":"en","primary_location":{"id":"doi:10.1093/bioinformatics/btt672","is_oa":true,"landing_page_url":"https://doi.org/10.1093/bioinformatics/btt672","pdf_url":"https://academic.oup.com/bioinformatics/article-pdf/30/7/981/17147782/btt672.pdf","source":{"id":"https://openalex.org/S52395412","display_name":"Bioinformatics","issn_l":"1367-4803","issn":["1367-4803","1367-4811"],"is_oa":false,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Bioinformatics","raw_type":"journal-article"},"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"https://academic.oup.com/bioinformatics/article-pdf/30/7/981/17147782/btt672.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5056664497","display_name":"Satoshi Ohno","orcid":"https://orcid.org/0000-0002-5642-8167"},"institutions":[{"id":"https://openalex.org/I98285908","display_name":"The University of Osaka","ror":"https://ror.org/035t8zc32","country_code":"JP","type":"education","lineage":["https://openalex.org/I98285908"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Satoshi Ohno","raw_affiliation_strings":["1 Department of Bioinformatic Engineering, Graduate School of Information Science and Technology, Osaka University, 1-5 Yamadaoka, Suita, Osaka 565-0871 and 2Quantitative Biology Center (QBiC), RIKEN, 6-2-3 Furuedai, Suita, Osaka 565-0874, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1 Department of Bioinformatic Engineering, Graduate School of Information Science and Technology, Osaka University, 1-5 Yamadaoka, Suita, Osaka 565-0871 and 2Quantitative Biology Center (QBiC), RIKEN, 6-2-3 Furuedai, Suita, Osaka 565-0874, Japan","institution_ids":["https://openalex.org/I98285908"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5057494257","display_name":"Hiroshi Shimizu","orcid":"https://orcid.org/0000-0002-8986-0861"},"institutions":[{"id":"https://openalex.org/I98285908","display_name":"The University of Osaka","ror":"https://ror.org/035t8zc32","country_code":"JP","type":"education","lineage":["https://openalex.org/I98285908"]}],"countries":["JP"],"is_corresponding":true,"raw_author_name":"Hiroshi Shimizu","raw_affiliation_strings":["1 Department of Bioinformatic Engineering, Graduate School of Information Science and Technology, Osaka University, 1-5 Yamadaoka, Suita, Osaka 565-0871 and 2Quantitative Biology Center (QBiC), RIKEN, 6-2-3 Furuedai, Suita, Osaka 565-0874, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1 Department of Bioinformatic Engineering, Graduate School of Information Science and Technology, Osaka University, 1-5 Yamadaoka, Suita, Osaka 565-0871 and 2Quantitative Biology Center (QBiC), RIKEN, 6-2-3 Furuedai, Suita, Osaka 565-0874, Japan","institution_ids":["https://openalex.org/I98285908"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5084886439","display_name":"Chikara Furusawa","orcid":"https://orcid.org/0000-0003-3554-4975"},"institutions":[{"id":"https://openalex.org/I98285908","display_name":"The University of Osaka","ror":"https://ror.org/035t8zc32","country_code":"JP","type":"education","lineage":["https://openalex.org/I98285908"]}],"countries":["JP"],"is_corresponding":true,"raw_author_name":"Chikara Furusawa","raw_affiliation_strings":["1 Department of Bioinformatic Engineering, Graduate School of Information Science and Technology, Osaka University, 1-5 Yamadaoka, Suita, Osaka 565-0871 and 2Quantitative Biology Center (QBiC), RIKEN, 6-2-3 Furuedai, Suita, Osaka 565-0874, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1 Department of Bioinformatic Engineering, Graduate School of Information Science and Technology, Osaka University, 1-5 Yamadaoka, Suita, Osaka 565-0871 and 2Quantitative Biology Center (QBiC), RIKEN, 6-2-3 Furuedai, Suita, Osaka 565-0874, Japan","institution_ids":["https://openalex.org/I98285908"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5057494257","https://openalex.org/A5084886439"],"corresponding_institution_ids":["https://openalex.org/I98285908"],"apc_list":{"value":3989,"currency":"USD","value_usd":3989},"apc_paid":{"value":3989,"currency":"USD","value_usd":3989},"fwci":1.7867,"has_fulltext":true,"cited_by_count":54,"citation_normalized_percentile":{"value":0.84988808,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":94,"max":99},"biblio":{"volume":"30","issue":"7","first_page":"981","last_page":"987"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.9957000017166138,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.9957000017166138,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10171","display_name":"Biofuel production and bioconversion","score":0.0005000000237487257,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.0005000000237487257,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/gene-knockout","display_name":"Gene knockout","score":0.7958738803863525},{"id":"https://openalex.org/keywords/computer-science","display_name":"Computer science","score":0.6214646100997925},{"id":"https://openalex.org/keywords/toolbox","display_name":"Toolbox","score":0.5211746096611023},{"id":"https://openalex.org/keywords/metabolite","display_name":"Metabolite","score":0.5009152889251709},{"id":"https://openalex.org/keywords/flux","display_name":"Flux (metallurgy)","score":0.49498194456100464},{"id":"https://openalex.org/keywords/computational-biology","display_name":"Computational biology","score":0.4857233166694641},{"id":"https://openalex.org/keywords/flux-balance-analysis","display_name":"Flux balance analysis","score":0.46534430980682373},{"id":"https://openalex.org/keywords/maximization","display_name":"Maximization","score":0.46268072724342346},{"id":"https://openalex.org/keywords/metabolic-engineering","display_name":"Metabolic engineering","score":0.4378502666950226},{"id":"https://openalex.org/keywords/constraint","display_name":"Constraint (computer-aided design)","score":0.4248931407928467},{"id":"https://openalex.org/keywords/set","display_name":"Set (abstract data type)","score":0.4235732853412628},{"id":"https://openalex.org/keywords/biology","display_name":"Biology","score":0.22440502047538757},{"id":"https://openalex.org/keywords/chemistry","display_name":"Chemistry","score":0.21640992164611816},{"id":"https://openalex.org/keywords/biochemistry","display_name":"Biochemistry","score":0.1788705587387085},{"id":"https://openalex.org/keywords/gene","display_name":"Gene","score":0.17607742547988892},{"id":"https://openalex.org/keywords/mathematics","display_name":"Mathematics","score":0.1259154975414276},{"id":"https://openalex.org/keywords/mathematical-optimization","display_name":"Mathematical optimization","score":0.12443694472312927}],"concepts":[{"id":"https://openalex.org/C77957584","wikidata":"https://www.wikidata.org/wiki/Q1133550","display_name":"Gene knockout","level":3,"score":0.7958738803863525},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.6214646100997925},{"id":"https://openalex.org/C2777655017","wikidata":"https://www.wikidata.org/wiki/Q1501161","display_name":"Toolbox","level":2,"score":0.5211746096611023},{"id":"https://openalex.org/C2777477808","wikidata":"https://www.wikidata.org/wiki/Q407595","display_name":"Metabolite","level":2,"score":0.5009152889251709},{"id":"https://openalex.org/C68709404","wikidata":"https://www.wikidata.org/wiki/Q1134475","display_name":"Flux (metallurgy)","level":2,"score":0.49498194456100464},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.4857233166694641},{"id":"https://openalex.org/C167091322","wikidata":"https://www.wikidata.org/wiki/Q5463029","display_name":"Flux balance analysis","level":2,"score":0.46534430980682373},{"id":"https://openalex.org/C2776330181","wikidata":"https://www.wikidata.org/wiki/Q18358244","display_name":"Maximization","level":2,"score":0.46268072724342346},{"id":"https://openalex.org/C6350086","wikidata":"https://www.wikidata.org/wiki/Q6822347","display_name":"Metabolic engineering","level":3,"score":0.4378502666950226},{"id":"https://openalex.org/C2776036281","wikidata":"https://www.wikidata.org/wiki/Q48769818","display_name":"Constraint (computer-aided design)","level":2,"score":0.4248931407928467},{"id":"https://openalex.org/C177264268","wikidata":"https://www.wikidata.org/wiki/Q1514741","display_name":"Set (abstract data type)","level":2,"score":0.4235732853412628},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.22440502047538757},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.21640992164611816},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.1788705587387085},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.17607742547988892},{"id":"https://openalex.org/C33923547","wikidata":"https://www.wikidata.org/wiki/Q395","display_name":"Mathematics","level":0,"score":0.1259154975414276},{"id":"https://openalex.org/C126255220","wikidata":"https://www.wikidata.org/wiki/Q141495","display_name":"Mathematical optimization","level":1,"score":0.12443694472312927},{"id":"https://openalex.org/C199360897","wikidata":"https://www.wikidata.org/wiki/Q9143","display_name":"Programming language","level":1,"score":0.0},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0},{"id":"https://openalex.org/C2524010","wikidata":"https://www.wikidata.org/wiki/Q8087","display_name":"Geometry","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000465","descriptor_name":"Algorithms","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000465","descriptor_name":"Algorithms","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000465","descriptor_name":"Algorithms","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D016000","descriptor_name":"Cluster Analysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016000","descriptor_name":"Cluster Analysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016000","descriptor_name":"Cluster Analysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016680","descriptor_name":"Genome, Bacterial","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016680","descriptor_name":"Genome, Bacterial","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016680","descriptor_name":"Genome, Bacterial","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D053858","descriptor_name":"Metabolic Networks and Pathways","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D053858","descriptor_name":"Metabolic Networks and Pathways","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D053858","descriptor_name":"Metabolic Networks and Pathways","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D060847","descriptor_name":"Metabolic Engineering","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D060847","descriptor_name":"Metabolic Engineering","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D060847","descriptor_name":"Metabolic Engineering","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1093/bioinformatics/btt672","is_oa":true,"landing_page_url":"https://doi.org/10.1093/bioinformatics/btt672","pdf_url":"https://academic.oup.com/bioinformatics/article-pdf/30/7/981/17147782/btt672.pdf","source":{"id":"https://openalex.org/S52395412","display_name":"Bioinformatics","issn_l":"1367-4803","issn":["1367-4803","1367-4811"],"is_oa":false,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Bioinformatics","raw_type":"journal-article"},{"id":"pmid:24257186","is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/24257186","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Bioinformatics (Oxford, England)","raw_type":"Journal Article"},{"id":"pmh:oai:europepmc.org:2969375","is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3967105","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Text"}],"best_oa_location":{"id":"doi:10.1093/bioinformatics/btt672","is_oa":true,"landing_page_url":"https://doi.org/10.1093/bioinformatics/btt672","pdf_url":"https://academic.oup.com/bioinformatics/article-pdf/30/7/981/17147782/btt672.pdf","source":{"id":"https://openalex.org/S52395412","display_name":"Bioinformatics","issn_l":"1367-4803","issn":["1367-4803","1367-4811"],"is_oa":false,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Bioinformatics","raw_type":"journal-article"},"sustainable_development_goals":[{"display_name":"Decent work and economic growth","score":0.49000000953674316,"id":"https://metadata.un.org/sdg/8"}],"awards":[{"id":"https://openalex.org/G2565625886","display_name":"Analysis of adaptive evolution using an automated culture system of E. coli","funder_award_id":"23680030","funder_id":"https://openalex.org/F4320334764","funder_display_name":"Japan Society for the Promotion of Science"},{"id":"https://openalex.org/G504332989","display_name":null,"funder_award_id":"25128715","funder_id":"https://openalex.org/F4320334764","funder_display_name":"Japan Society for the Promotion of Science"},{"id":"https://openalex.org/G6029168926","display_name":"Creation of Stress Tolerant Microbial Cell Factories based on Multiomics Analysis of Laboratory Evolution Process","funder_award_id":"24246134","funder_id":"https://openalex.org/F4320334764","funder_display_name":"Japan Society for the Promotion of Science"}],"funders":[{"id":"https://openalex.org/F4320334764","display_name":"Japan Society for the Promotion of Science","ror":"https://ror.org/00hhkn466"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2131629152.pdf","grobid_xml":"https://content.openalex.org/works/W2131629152.grobid-xml"},"referenced_works_count":38,"referenced_works":["https://openalex.org/W147290053","https://openalex.org/W1574470162","https://openalex.org/W1969983517","https://openalex.org/W1972549326","https://openalex.org/W1979287129","https://openalex.org/W1980875662","https://openalex.org/W1988051581","https://openalex.org/W1996629685","https://openalex.org/W1998814361","https://openalex.org/W2004623533","https://openalex.org/W2004964623","https://openalex.org/W2006796424","https://openalex.org/W2027251495","https://openalex.org/W2048601646","https://openalex.org/W2054506883","https://openalex.org/W2056305771","https://openalex.org/W2062819025","https://openalex.org/W2071524536","https://openalex.org/W2076262841","https://openalex.org/W2078355959","https://openalex.org/W2078833660","https://openalex.org/W2083011190","https://openalex.org/W2086320398","https://openalex.org/W2098824895","https://openalex.org/W2100818174","https://openalex.org/W2109880272","https://openalex.org/W2111967267","https://openalex.org/W2113531097","https://openalex.org/W2128746754","https://openalex.org/W2130364940","https://openalex.org/W2136832197","https://openalex.org/W2143861551","https://openalex.org/W2146126338","https://openalex.org/W2149001103","https://openalex.org/W2156409537","https://openalex.org/W2160541874","https://openalex.org/W2165612309","https://openalex.org/W2170427655"],"related_works":["https://openalex.org/W1984577552","https://openalex.org/W1918238777","https://openalex.org/W1999460937","https://openalex.org/W2135476401","https://openalex.org/W2056305771","https://openalex.org/W2022041790","https://openalex.org/W2051292707","https://openalex.org/W2294839595","https://openalex.org/W2081052511","https://openalex.org/W2163823651"],"abstract_inverted_index":{"MOTIVATION:":[0],"Although":[1],"constraint-based":[2],"flux":[3,69],"analysis":[4],"of":[5,12,48,55,64,86,107],"knockout":[6,52,80,90],"strains":[7],"has":[8],"facilitated":[9],"the":[10,25,46,65,68,84,97],"production":[11,54],"desirable":[13],"metabolites":[14,109],"in":[15,67,96,130,154],"microbes,":[16],"current":[17],"screening":[18,39,75,134],"methods":[19],"have":[20],"placed":[21],"a":[22,37,49,56,74],"limitation":[23],"on":[24],"number":[26],"knockouts":[27],"that":[28,105,127],"can":[29],"be":[30,111],"simultaneously":[31],"analyzed.":[32],"RESULTS:":[33],"Here,":[34],"we":[35,88,125],"propose":[36],"novel":[38],"method":[40],"named":[41],"FastPros.":[42],"In":[43],"this":[44],"method,":[45,135],"potential":[47],"given":[50],"reaction":[51,122],"for":[53],"specific":[57],"metabolite":[58,95,141],"is":[59,148],"evaluated":[60],"by":[61,117],"shadow":[62],"pricing":[63],"constraint":[66],"balance":[70],"analysis,":[71],"which":[72],"generates":[73],"score":[76],"to":[77,92,120],"obtain":[78],"candidate":[79],"sets.":[81],"To":[82],"evaluate":[83],"performance":[85],"FastPros,":[87],"screened":[89],"sets":[91],"produce":[93],"each":[94],"entire":[98],"Escherichia":[99],"coli":[100],"metabolic":[101],"network.":[102],"We":[103],"found":[104],"75%":[106],"these":[108],"could":[110,137],"produced":[112],"under":[113],"biomass":[114],"maximization":[115],"conditions":[116],"adding":[118],"up":[119],"25":[121],"knockouts.":[123],"Furthermore,":[124],"demonstrated":[126],"using":[128],"FastPros":[129],"tandem":[131],"with":[132],"another":[133],"OptKnock,":[136],"further":[138],"improve":[139],"target":[140],"productivity.":[142],"AVAILABILITY":[143],"AND":[144],"IMPLEMENTATION:":[145],"Source":[146],"code":[147],"freely":[149],"available":[150],"at":[151],"http://www-shimizu.ist.osaka-u.ac.jp/shimizu_lab/FastPros/,":[152],"implemented":[153],"MATLAB":[155],"and":[156],"COBRA":[157],"toolbox.":[158]},"counts_by_year":[{"year":2026,"cited_by_count":2},{"year":2025,"cited_by_count":5},{"year":2024,"cited_by_count":3},{"year":2023,"cited_by_count":2},{"year":2022,"cited_by_count":5},{"year":2021,"cited_by_count":5},{"year":2020,"cited_by_count":2},{"year":2019,"cited_by_count":4},{"year":2018,"cited_by_count":4},{"year":2017,"cited_by_count":9},{"year":2016,"cited_by_count":4},{"year":2015,"cited_by_count":5},{"year":2014,"cited_by_count":4}],"updated_date":"2026-08-21T09:56:20.448147","created_date":"2025-10-10T00:00:00"}