{"id":"https://openalex.org/W2137460766","doi":"https://doi.org/10.1093/bioinformatics/bts714","title":"Data exploration, quality control and testing in single-cell qPCR-based gene expression experiments","display_name":"Data exploration, quality control and testing in single-cell qPCR-based gene expression experiments","publication_year":2012,"publication_date":"2012-12-24","ids":{"openalex":"https://openalex.org/W2137460766","doi":"https://doi.org/10.1093/bioinformatics/bts714","mag":"2137460766","pmid":"https://pubmed.ncbi.nlm.nih.gov/23267174"},"language":"en","primary_location":{"id":"doi:10.1093/bioinformatics/bts714","is_oa":true,"landing_page_url":"https://doi.org/10.1093/bioinformatics/bts714","pdf_url":null,"source":{"id":"https://openalex.org/S52395412","display_name":"Bioinformatics","issn_l":"1367-4803","issn":["1367-4803","1367-4811"],"is_oa":false,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Bioinformatics","raw_type":"journal-article"},"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"https://doi.org/10.1093/bioinformatics/bts714","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5063304924","display_name":"Andrew McDavid","orcid":"https://orcid.org/0000-0002-6581-1213"},"institutions":[{"id":"https://openalex.org/I201448701","display_name":"University of Washington","ror":"https://ror.org/00cvxb145","country_code":"US","type":"education","lineage":["https://openalex.org/I201448701"]},{"id":"https://openalex.org/I4210089486","display_name":"Fred Hutch Cancer Center","ror":"https://ror.org/007ps6h72","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I4210089486"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Andrew McDavid","raw_affiliation_strings":["1 Department of Statistics, University of Washington, Seattle, WA 98195, USA, 2Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA, 3ImmunoTechnology Section, Vaccine Research Center, NIAID, NIH, Bethesda, MD 20892, USA and 4Immunology Laboratory, Vaccine Research Center, NIAID, NIH, Bethesda, MD 20892, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1 Department of Statistics, University of Washington, Seattle, WA 98195, USA, 2Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA, 3ImmunoTechnology Section, Vaccine Research Center, NIAID, NIH, Bethesda, MD 20892, USA and 4Immunology Laboratory, Vaccine Research Center, NIAID, NIH, Bethesda, MD 20892, USA","institution_ids":["https://openalex.org/I201448701","https://openalex.org/I4210089486"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5069196767","display_name":"Greg Finak","orcid":"https://orcid.org/0000-0003-4341-9090"},"institutions":[{"id":"https://openalex.org/I201448701","display_name":"University of Washington","ror":"https://ror.org/00cvxb145","country_code":"US","type":"education","lineage":["https://openalex.org/I201448701"]},{"id":"https://openalex.org/I4210089486","display_name":"Fred Hutch Cancer Center","ror":"https://ror.org/007ps6h72","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I4210089486"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Greg Finak","raw_affiliation_strings":["1 Department of Statistics, University of Washington, Seattle, WA 98195, USA, 2Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA, 3ImmunoTechnology Section, Vaccine Research Center, NIAID, NIH, Bethesda, MD 20892, USA and 4Immunology Laboratory, Vaccine Research Center, NIAID, NIH, Bethesda, MD 20892, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1 Department of Statistics, University of Washington, Seattle, WA 98195, USA, 2Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA, 3ImmunoTechnology Section, Vaccine Research Center, NIAID, NIH, Bethesda, MD 20892, USA and 4Immunology Laboratory, Vaccine Research Center, NIAID, NIH, Bethesda, MD 20892, USA","institution_ids":["https://openalex.org/I201448701","https://openalex.org/I4210089486"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5112866897","display_name":"Pratip K. Chattopadyay","orcid":null},"institutions":[{"id":"https://openalex.org/I201448701","display_name":"University of Washington","ror":"https://ror.org/00cvxb145","country_code":"US","type":"education","lineage":["https://openalex.org/I201448701"]},{"id":"https://openalex.org/I4210089486","display_name":"Fred Hutch Cancer Center","ror":"https://ror.org/007ps6h72","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I4210089486"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Pratip K. Chattopadyay","raw_affiliation_strings":["1 Department of Statistics, University of Washington, Seattle, WA 98195, USA, 2Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA, 3ImmunoTechnology Section, Vaccine Research Center, NIAID, NIH, Bethesda, MD 20892, USA and 4Immunology Laboratory, Vaccine Research Center, NIAID, NIH, Bethesda, MD 20892, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1 Department of Statistics, University of Washington, Seattle, WA 98195, USA, 2Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA, 3ImmunoTechnology Section, Vaccine Research Center, NIAID, NIH, Bethesda, MD 20892, USA and 4Immunology Laboratory, Vaccine Research Center, NIAID, NIH, Bethesda, MD 20892, USA","institution_ids":["https://openalex.org/I201448701","https://openalex.org/I4210089486"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5023507930","display_name":"Mar\u00eda Dom\u00ednguez","orcid":"https://orcid.org/0000-0002-3329-7862"},"institutions":[{"id":"https://openalex.org/I201448701","display_name":"University of Washington","ror":"https://ror.org/00cvxb145","country_code":"US","type":"education","lineage":["https://openalex.org/I201448701"]},{"id":"https://openalex.org/I4210089486","display_name":"Fred Hutch Cancer Center","ror":"https://ror.org/007ps6h72","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I4210089486"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Maria Dominguez","raw_affiliation_strings":["1 Department of Statistics, University of Washington, Seattle, WA 98195, USA, 2Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA, 3ImmunoTechnology Section, Vaccine Research Center, NIAID, NIH, Bethesda, MD 20892, USA and 4Immunology Laboratory, Vaccine Research Center, NIAID, NIH, Bethesda, MD 20892, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1 Department of Statistics, University of Washington, Seattle, WA 98195, USA, 2Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA, 3ImmunoTechnology Section, Vaccine Research Center, NIAID, NIH, Bethesda, MD 20892, USA and 4Immunology Laboratory, Vaccine Research Center, NIAID, NIH, Bethesda, MD 20892, USA","institution_ids":["https://openalex.org/I201448701","https://openalex.org/I4210089486"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5057692531","display_name":"Laurie Lamoreaux","orcid":null},"institutions":[{"id":"https://openalex.org/I201448701","display_name":"University of Washington","ror":"https://ror.org/00cvxb145","country_code":"US","type":"education","lineage":["https://openalex.org/I201448701"]},{"id":"https://openalex.org/I4210089486","display_name":"Fred Hutch Cancer Center","ror":"https://ror.org/007ps6h72","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I4210089486"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Laurie Lamoreaux","raw_affiliation_strings":["1 Department of Statistics, University of Washington, Seattle, WA 98195, USA, 2Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA, 3ImmunoTechnology Section, Vaccine Research Center, NIAID, NIH, Bethesda, MD 20892, USA and 4Immunology Laboratory, Vaccine Research Center, NIAID, NIH, Bethesda, MD 20892, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1 Department of Statistics, University of Washington, Seattle, WA 98195, USA, 2Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA, 3ImmunoTechnology Section, Vaccine Research Center, NIAID, NIH, Bethesda, MD 20892, USA and 4Immunology Laboratory, Vaccine Research Center, NIAID, NIH, Bethesda, MD 20892, USA","institution_ids":["https://openalex.org/I201448701","https://openalex.org/I4210089486"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5113956487","display_name":"Schmidt Steven","orcid":null},"institutions":[{"id":"https://openalex.org/I201448701","display_name":"University of Washington","ror":"https://ror.org/00cvxb145","country_code":"US","type":"education","lineage":["https://openalex.org/I201448701"]},{"id":"https://openalex.org/I4210089486","display_name":"Fred Hutch Cancer Center","ror":"https://ror.org/007ps6h72","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I4210089486"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Steven S. Ma","raw_affiliation_strings":["1 Department of Statistics, University of Washington, Seattle, WA 98195, USA, 2Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA, 3ImmunoTechnology Section, Vaccine Research Center, NIAID, NIH, Bethesda, MD 20892, USA and 4Immunology Laboratory, Vaccine Research Center, NIAID, NIH, Bethesda, MD 20892, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1 Department of Statistics, University of Washington, Seattle, WA 98195, USA, 2Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA, 3ImmunoTechnology Section, Vaccine Research Center, NIAID, NIH, Bethesda, MD 20892, USA and 4Immunology Laboratory, Vaccine Research Center, NIAID, NIH, Bethesda, MD 20892, USA","institution_ids":["https://openalex.org/I201448701","https://openalex.org/I4210089486"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5090939942","display_name":"Mario Roederer","orcid":"https://orcid.org/0000-0001-7448-7040"},"institutions":[{"id":"https://openalex.org/I201448701","display_name":"University of Washington","ror":"https://ror.org/00cvxb145","country_code":"US","type":"education","lineage":["https://openalex.org/I201448701"]},{"id":"https://openalex.org/I4210089486","display_name":"Fred Hutch Cancer Center","ror":"https://ror.org/007ps6h72","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I4210089486"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Mario Roederer","raw_affiliation_strings":["1 Department of Statistics, University of Washington, Seattle, WA 98195, USA, 2Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA, 3ImmunoTechnology Section, Vaccine Research Center, NIAID, NIH, Bethesda, MD 20892, USA and 4Immunology Laboratory, Vaccine Research Center, NIAID, NIH, Bethesda, MD 20892, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1 Department of Statistics, University of Washington, Seattle, WA 98195, USA, 2Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA, 3ImmunoTechnology Section, Vaccine Research Center, NIAID, NIH, Bethesda, MD 20892, USA and 4Immunology Laboratory, Vaccine Research Center, NIAID, NIH, Bethesda, MD 20892, USA","institution_ids":["https://openalex.org/I201448701","https://openalex.org/I4210089486"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5005741024","display_name":"Rapha\u00ebl Gottardo","orcid":"https://orcid.org/0000-0002-3867-0232"},"institutions":[{"id":"https://openalex.org/I201448701","display_name":"University of Washington","ror":"https://ror.org/00cvxb145","country_code":"US","type":"education","lineage":["https://openalex.org/I201448701"]},{"id":"https://openalex.org/I4210089486","display_name":"Fred Hutch Cancer Center","ror":"https://ror.org/007ps6h72","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I4210089486"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Raphael Gottardo","raw_affiliation_strings":["1 Department of Statistics, University of Washington, Seattle, WA 98195, USA, 2Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA, 3ImmunoTechnology Section, Vaccine Research Center, NIAID, NIH, Bethesda, MD 20892, USA and 4Immunology Laboratory, Vaccine Research Center, NIAID, NIH, Bethesda, MD 20892, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1 Department of Statistics, University of Washington, Seattle, WA 98195, USA, 2Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA, 3ImmunoTechnology Section, Vaccine Research Center, NIAID, NIH, Bethesda, MD 20892, USA and 4Immunology Laboratory, Vaccine Research Center, NIAID, NIH, Bethesda, MD 20892, USA","institution_ids":["https://openalex.org/I201448701","https://openalex.org/I4210089486"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5005741024"],"corresponding_institution_ids":["https://openalex.org/I201448701","https://openalex.org/I4210089486"],"apc_list":{"value":3989,"currency":"USD","value_usd":3989},"apc_paid":{"value":3989,"currency":"USD","value_usd":3989},"fwci":5.658,"has_fulltext":false,"cited_by_count":450,"citation_normalized_percentile":{"value":0.97169127,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":89,"max":100},"biblio":{"volume":"29","issue":"4","first_page":"461","last_page":"467"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11289","display_name":"Single-cell and spatial transcriptomics","score":0.7303000092506409,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11289","display_name":"Single-cell and spatial transcriptomics","score":0.7303000092506409,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10885","display_name":"Gene expression and cancer classification","score":0.06509999930858612,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.054099999368190765,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/computer-science","display_name":"Computer science","score":0.6077533960342407},{"id":"https://openalex.org/keywords/expression","display_name":"Expression (computer science)","score":0.5570070147514343},{"id":"https://openalex.org/keywords/parametric-statistics","display_name":"Parametric statistics","score":0.5289984345436096},{"id":"https://openalex.org/keywords/computational-biology","display_name":"Computational biology","score":0.5141394734382629},{"id":"https://openalex.org/keywords/statistical-hypothesis-testing","display_name":"Statistical hypothesis testing","score":0.48061603307724},{"id":"https://openalex.org/keywords/data-mining","display_name":"Data mining","score":0.44130805134773254},{"id":"https://openalex.org/keywords/biology","display_name":"Biology","score":0.30441009998321533},{"id":"https://openalex.org/keywords/statistics","display_name":"Statistics","score":0.2876752018928528},{"id":"https://openalex.org/keywords/mathematics","display_name":"Mathematics","score":0.21137309074401855}],"concepts":[{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.6077533960342407},{"id":"https://openalex.org/C90559484","wikidata":"https://www.wikidata.org/wiki/Q778379","display_name":"Expression (computer science)","level":2,"score":0.5570070147514343},{"id":"https://openalex.org/C117251300","wikidata":"https://www.wikidata.org/wiki/Q1849855","display_name":"Parametric statistics","level":2,"score":0.5289984345436096},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.5141394734382629},{"id":"https://openalex.org/C87007009","wikidata":"https://www.wikidata.org/wiki/Q210832","display_name":"Statistical hypothesis testing","level":2,"score":0.48061603307724},{"id":"https://openalex.org/C124101348","wikidata":"https://www.wikidata.org/wiki/Q172491","display_name":"Data mining","level":1,"score":0.44130805134773254},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.30441009998321533},{"id":"https://openalex.org/C105795698","wikidata":"https://www.wikidata.org/wiki/Q12483","display_name":"Statistics","level":1,"score":0.2876752018928528},{"id":"https://openalex.org/C33923547","wikidata":"https://www.wikidata.org/wiki/Q395","display_name":"Mathematics","level":0,"score":0.21137309074401855},{"id":"https://openalex.org/C199360897","wikidata":"https://www.wikidata.org/wiki/Q9143","display_name":"Programming language","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011786","descriptor_name":"Quality Control","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011786","descriptor_name":"Quality Control","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011786","descriptor_name":"Quality Control","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011786","descriptor_name":"Quality Control","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015233","descriptor_name":"Models, Statistical","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015233","descriptor_name":"Models, Statistical","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015233","descriptor_name":"Models, Statistical","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015233","descriptor_name":"Models, Statistical","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D020133","descriptor_name":"Reverse Transcriptase Polymerase Chain Reaction","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D020133","descriptor_name":"Reverse Transcriptase Polymerase Chain Reaction","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D020133","descriptor_name":"Reverse Transcriptase Polymerase Chain Reaction","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D020133","descriptor_name":"Reverse Transcriptase Polymerase Chain Reaction","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D020869","descriptor_name":"Gene Expression Profiling","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D020869","descriptor_name":"Gene Expression Profiling","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D020869","descriptor_name":"Gene Expression Profiling","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D020869","descriptor_name":"Gene Expression Profiling","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D020869","descriptor_name":"Gene Expression Profiling","qualifier_ui":"Q000592","qualifier_name":"standards","is_major_topic":false},{"descriptor_ui":"D020869","descriptor_name":"Gene Expression Profiling","qualifier_ui":"Q000592","qualifier_name":"standards","is_major_topic":false},{"descriptor_ui":"D020869","descriptor_name":"Gene Expression Profiling","qualifier_ui":"Q000592","qualifier_name":"standards","is_major_topic":false},{"descriptor_ui":"D020869","descriptor_name":"Gene Expression Profiling","qualifier_ui":"Q000592","qualifier_name":"standards","is_major_topic":false},{"descriptor_ui":"D046210","descriptor_name":"Microfluidic Analytical Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D046210","descriptor_name":"Microfluidic Analytical Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D046210","descriptor_name":"Microfluidic Analytical Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D046210","descriptor_name":"Microfluidic Analytical Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D059010","descriptor_name":"Single-Cell Analysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D059010","descriptor_name":"Single-Cell Analysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D059010","descriptor_name":"Single-Cell Analysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D059010","descriptor_name":"Single-Cell Analysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1093/bioinformatics/bts714","is_oa":true,"landing_page_url":"https://doi.org/10.1093/bioinformatics/bts714","pdf_url":null,"source":{"id":"https://openalex.org/S52395412","display_name":"Bioinformatics","issn_l":"1367-4803","issn":["1367-4803","1367-4811"],"is_oa":false,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Bioinformatics","raw_type":"journal-article"},{"id":"pmid:23267174","is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/23267174","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Bioinformatics (Oxford, England)","raw_type":"Journal Article"},{"id":"pmh:oai:europepmc.org:2571358","is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3570210","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Text"}],"best_oa_location":{"id":"doi:10.1093/bioinformatics/bts714","is_oa":true,"landing_page_url":"https://doi.org/10.1093/bioinformatics/bts714","pdf_url":null,"source":{"id":"https://openalex.org/S52395412","display_name":"Bioinformatics","issn_l":"1367-4803","issn":["1367-4803","1367-4811"],"is_oa":false,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Bioinformatics","raw_type":"journal-article"},"sustainable_development_goals":[],"awards":[{"id":"https://openalex.org/G3181052152","display_name":null,"funder_award_id":"U19 AI089986-01","funder_id":"https://openalex.org/F4320337355","funder_display_name":"National Institute of Allergy and Infectious Diseases"},{"id":"https://openalex.org/G4793338163","display_name":null,"funder_award_id":"R01 EB008400","funder_id":"https://openalex.org/F4320337363","funder_display_name":"National Institute of Biomedical Imaging and Bioengineering"}],"funders":[{"id":"https://openalex.org/F4320337355","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69"},{"id":"https://openalex.org/F4320337363","display_name":"National Institute of Biomedical Imaging and Bioengineering","ror":"https://ror.org/00372qc85"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":19,"referenced_works":["https://openalex.org/W1577577364","https://openalex.org/W1984984201","https://openalex.org/W2017325329","https://openalex.org/W2019490156","https://openalex.org/W2019841176","https://openalex.org/W2021429824","https://openalex.org/W2022227920","https://openalex.org/W2073857965","https://openalex.org/W2081098333","https://openalex.org/W2081859209","https://openalex.org/W2087242711","https://openalex.org/W2113381189","https://openalex.org/W2114570899","https://openalex.org/W2115463832","https://openalex.org/W2116053761","https://openalex.org/W2116129645","https://openalex.org/W2121690903","https://openalex.org/W2128416381","https://openalex.org/W2313339984"],"related_works":["https://openalex.org/W2392243736","https://openalex.org/W86652014","https://openalex.org/W2328518092","https://openalex.org/W3101249758","https://openalex.org/W2382079200","https://openalex.org/W2374091470","https://openalex.org/W1902660783","https://openalex.org/W4388218547","https://openalex.org/W2379703849","https://openalex.org/W4312411653"],"abstract_inverted_index":{"MOTIVATION:":[0],"Cell":[1],"populations":[2],"are":[3,206],"never":[4],"truly":[5],"homogeneous;":[6],"individual":[7],"cells":[8],"exist":[9],"in":[10,168,186],"biochemical":[11],"states":[12],"that":[13,114,153,164,172],"define":[14],"functional":[15],"differences":[16],"between":[17],"them.":[18],"New":[19],"technology":[20],"based":[21],"on":[22,122,140,191,230],"microfluidic":[23,84],"arrays":[24],"combined":[25,146,174],"with":[26],"multiplexed":[27],"quantitative":[28,60],"polymerase":[29,61],"chain":[30,62],"reactions":[31,63],"now":[32],"enables":[33],"high-throughput":[34],"single-cell":[35,59,79,93,118],"gene":[36,80],"expression":[37,81,94,126,136,152],"measurement,":[38],"allowing":[39],"assessment":[40],"of":[41,58,78,92,215],"cellular":[42],"heterogeneity.":[43],"However,":[44],"few":[45],"analytic":[46],"tools":[47,205],"have":[48],"been":[49],"developed":[50,196],"specifically":[51],"for":[52,71,111,150,197],"the":[53,72,112,117,134,156,181,192,225],"statistical":[54,69,108],"and":[55,76,89,95,158],"analytical":[56],"challenges":[57],"data.":[64,194],"RESULTS:":[65],"We":[66,86,105],"present":[67],"a":[68,107,124,145,189,200],"framework":[70],"exploration,":[73],"quality":[74,97],"control":[75,98],"analysis":[77],"data":[82],"from":[83,199],"arrays.":[85],"assess":[87],"accuracy":[88],"within-sample":[90],"heterogeneity":[91],"develop":[96],"criteria":[99],"to":[100,208],"filter":[101],"unreliable":[102],"cell":[103],"measurements.":[104],"propose":[106],"model":[109],"accounting":[110],"fact":[113],"genes":[115],"at":[116],"level":[119],"can":[120],"be":[121],"(and":[123,133],"continuous":[125,159,182],"measure":[127],"is":[128,137,176],"recorded)":[129],"or":[130,183,188],"dichotomously":[131],"off":[132],"recorded":[135],"zero).":[138],"Based":[139],"this":[141,173],"model,":[142],"we":[143,170],"derive":[144],"likelihood":[147],"ratio":[148],"test":[149,175],"differential":[151],"incorporates":[154],"both":[155],"discrete":[157],"components.":[160],"Using":[161],"an":[162],"experiment":[163],"examines":[165],"treatment-specific":[166],"changes":[167],"expression,":[169],"show":[171],"more":[177],"powerful":[178],"than":[179],"either":[180],"dichotomous":[184],"component":[185],"isolation,":[187],"t-test":[190],"zero-inflated":[193],"Although":[195],"measurements":[198],"specific":[201],"platform":[202],"(Fluidigm),":[203],"these":[204],"generalizable":[207],"other":[209],"multi-parametric":[210],"measures":[211],"over":[212],"large":[213],"numbers":[214],"events.":[216],"AVAILABILITY:":[217],"All":[218],"results":[219],"presented":[220],"here":[221],"were":[222],"obtained":[223],"using":[224],"SingleCellAssay":[226],"R":[227],"package":[228],"available":[229],"GitHub":[231],"(http://github.com/RGLab/SingleCellAssay).":[232]},"counts_by_year":[{"year":2026,"cited_by_count":10},{"year":2025,"cited_by_count":33},{"year":2024,"cited_by_count":28},{"year":2023,"cited_by_count":33},{"year":2022,"cited_by_count":46},{"year":2021,"cited_by_count":56},{"year":2020,"cited_by_count":53},{"year":2019,"cited_by_count":52},{"year":2018,"cited_by_count":38},{"year":2017,"cited_by_count":33},{"year":2016,"cited_by_count":22},{"year":2015,"cited_by_count":20},{"year":2014,"cited_by_count":20},{"year":2013,"cited_by_count":5},{"year":2012,"cited_by_count":1}],"updated_date":"2026-08-21T09:56:20.448147","created_date":"2025-10-10T00:00:00"}