{"id":"https://openalex.org/W3039872156","doi":"https://doi.org/10.1093/bioinformatics/btaa607","title":"Simulation, power evaluation and sample size recommendation for single-cell RNA-seq","display_name":"Simulation, power evaluation and sample size recommendation for single-cell RNA-seq","publication_year":2020,"publication_date":"2020-06-23","ids":{"openalex":"https://openalex.org/W3039872156","doi":"https://doi.org/10.1093/bioinformatics/btaa607","mag":"3039872156","pmid":"https://pubmed.ncbi.nlm.nih.gov/32614380"},"language":"en","primary_location":{"id":"doi:10.1093/bioinformatics/btaa607","is_oa":true,"landing_page_url":"https://doi.org/10.1093/bioinformatics/btaa607","pdf_url":"https://academic.oup.com/bioinformatics/article-pdf/36/19/4860/36040368/btaa607.pdf","source":{"id":"https://openalex.org/S52395412","display_name":"Bioinformatics","issn_l":"1367-4803","issn":["1367-4803","1367-4811"],"is_oa":false,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Bioinformatics","raw_type":"journal-article"},"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://academic.oup.com/bioinformatics/article-pdf/36/19/4860/36040368/btaa607.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5060121625","display_name":"Kenong Su","orcid":null},"institutions":[{"id":"https://openalex.org/I150468666","display_name":"Emory University","ror":"https://ror.org/03czfpz43","country_code":"US","type":"education","lineage":["https://openalex.org/I150468666"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Kenong Su","raw_affiliation_strings":["Department of Computer Science, Emory University , Atlanta, GA 30329, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Computer Science, Emory University , Atlanta, GA 30329, USA","institution_ids":["https://openalex.org/I150468666"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101770186","display_name":"Zhijin Wu","orcid":"https://orcid.org/0000-0002-9596-9134"},"institutions":[{"id":"https://openalex.org/I27804330","display_name":"Brown University","ror":"https://ror.org/05gq02987","country_code":"US","type":"education","lineage":["https://openalex.org/I27804330"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Zhijin Wu","raw_affiliation_strings":["Department of Biostatistics, Brown University , Providence, RI 02912, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biostatistics, Brown University , Providence, RI 02912, USA","institution_ids":["https://openalex.org/I27804330"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5083170497","display_name":"Hao Wu","orcid":"https://orcid.org/0000-0003-1269-7354"},"institutions":[{"id":"https://openalex.org/I150468666","display_name":"Emory University","ror":"https://ror.org/03czfpz43","country_code":"US","type":"education","lineage":["https://openalex.org/I150468666"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Hao Wu","raw_affiliation_strings":["Department of Biostatistics and Bioinformatics, Emory University , Atlanta, GA 30322, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biostatistics and Bioinformatics, Emory University , Atlanta, GA 30322, USA","institution_ids":["https://openalex.org/I150468666"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5083170497"],"corresponding_institution_ids":["https://openalex.org/I150468666"],"apc_list":{"value":3618,"currency":"USD","value_usd":3618},"apc_paid":null,"fwci":1.1931,"has_fulltext":false,"cited_by_count":38,"citation_normalized_percentile":{"value":0.77748075,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":97,"max":99},"biblio":{"volume":"36","issue":"19","first_page":"4860","last_page":"4868"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11289","display_name":"Single-cell and spatial transcriptomics","score":0.9995999932289124,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11289","display_name":"Single-cell and spatial transcriptomics","score":0.9995999932289124,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10885","display_name":"Gene expression and cancer classification","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11255","display_name":"Microfluidic and Bio-sensing Technologies","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/sample-size-determination","display_name":"Sample size determination","score":0.7497596740722656},{"id":"https://openalex.org/keywords/computer-science","display_name":"Computer science","score":0.747556746006012},{"id":"https://openalex.org/keywords/context","display_name":"Context (archaeology)","score":0.6068274974822998},{"id":"https://openalex.org/keywords/sample","display_name":"Sample (material)","score":0.5872278213500977},{"id":"https://openalex.org/keywords/statistical-power","display_name":"Statistical power","score":0.571990966796875},{"id":"https://openalex.org/keywords/data-mining","display_name":"Data mining","score":0.5648611187934875},{"id":"https://openalex.org/keywords/power-analysis","display_name":"Power analysis","score":0.5033945441246033},{"id":"https://openalex.org/keywords/throughput","display_name":"Throughput","score":0.446048378944397},{"id":"https://openalex.org/keywords/power","display_name":"Power (physics)","score":0.4213300049304962},{"id":"https://openalex.org/keywords/statistics","display_name":"Statistics","score":0.22330212593078613},{"id":"https://openalex.org/keywords/algorithm","display_name":"Algorithm","score":0.17669975757598877},{"id":"https://openalex.org/keywords/mathematics","display_name":"Mathematics","score":0.1061960756778717}],"concepts":[{"id":"https://openalex.org/C129848803","wikidata":"https://www.wikidata.org/wiki/Q2564360","display_name":"Sample size determination","level":2,"score":0.7497596740722656},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.747556746006012},{"id":"https://openalex.org/C2779343474","wikidata":"https://www.wikidata.org/wiki/Q3109175","display_name":"Context (archaeology)","level":2,"score":0.6068274974822998},{"id":"https://openalex.org/C198531522","wikidata":"https://www.wikidata.org/wiki/Q485146","display_name":"Sample (material)","level":2,"score":0.5872278213500977},{"id":"https://openalex.org/C96608239","wikidata":"https://www.wikidata.org/wiki/Q1199823","display_name":"Statistical power","level":2,"score":0.571990966796875},{"id":"https://openalex.org/C124101348","wikidata":"https://www.wikidata.org/wiki/Q172491","display_name":"Data mining","level":1,"score":0.5648611187934875},{"id":"https://openalex.org/C71743495","wikidata":"https://www.wikidata.org/wiki/Q2845210","display_name":"Power analysis","level":3,"score":0.5033945441246033},{"id":"https://openalex.org/C157764524","wikidata":"https://www.wikidata.org/wiki/Q1383412","display_name":"Throughput","level":3,"score":0.446048378944397},{"id":"https://openalex.org/C163258240","wikidata":"https://www.wikidata.org/wiki/Q25342","display_name":"Power (physics)","level":2,"score":0.4213300049304962},{"id":"https://openalex.org/C105795698","wikidata":"https://www.wikidata.org/wiki/Q12483","display_name":"Statistics","level":1,"score":0.22330212593078613},{"id":"https://openalex.org/C11413529","wikidata":"https://www.wikidata.org/wiki/Q8366","display_name":"Algorithm","level":1,"score":0.17669975757598877},{"id":"https://openalex.org/C33923547","wikidata":"https://www.wikidata.org/wiki/Q395","display_name":"Mathematics","level":0,"score":0.1061960756778717},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.0},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.0},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.0},{"id":"https://openalex.org/C62520636","wikidata":"https://www.wikidata.org/wiki/Q944","display_name":"Quantum mechanics","level":1,"score":0.0},{"id":"https://openalex.org/C555944384","wikidata":"https://www.wikidata.org/wiki/Q249","display_name":"Wireless","level":2,"score":0.0},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.0},{"id":"https://openalex.org/C151730666","wikidata":"https://www.wikidata.org/wiki/Q7205","display_name":"Paleontology","level":1,"score":0.0},{"id":"https://openalex.org/C178489894","wikidata":"https://www.wikidata.org/wiki/Q8789","display_name":"Cryptography","level":2,"score":0.0},{"id":"https://openalex.org/C76155785","wikidata":"https://www.wikidata.org/wiki/Q418","display_name":"Telecommunications","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000081246","descriptor_name":"RNA-Seq","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000081246","descriptor_name":"RNA-Seq","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000081246","descriptor_name":"RNA-Seq","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003198","descriptor_name":"Computer Simulation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003198","descriptor_name":"Computer Simulation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003198","descriptor_name":"Computer Simulation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012984","descriptor_name":"Software","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D012984","descriptor_name":"Software","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D012984","descriptor_name":"Software","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D017423","descriptor_name":"Sequence Analysis, RNA","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017423","descriptor_name":"Sequence Analysis, RNA","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017423","descriptor_name":"Sequence Analysis, RNA","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018401","descriptor_name":"Sample Size","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018401","descriptor_name":"Sample Size","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018401","descriptor_name":"Sample Size","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D020869","descriptor_name":"Gene Expression Profiling","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D020869","descriptor_name":"Gene Expression Profiling","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D020869","descriptor_name":"Gene Expression Profiling","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D059010","descriptor_name":"Single-Cell Analysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D059010","descriptor_name":"Single-Cell Analysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D059010","descriptor_name":"Single-Cell Analysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":3,"locations":[{"id":"doi:10.1093/bioinformatics/btaa607","is_oa":true,"landing_page_url":"https://doi.org/10.1093/bioinformatics/btaa607","pdf_url":"https://academic.oup.com/bioinformatics/article-pdf/36/19/4860/36040368/btaa607.pdf","source":{"id":"https://openalex.org/S52395412","display_name":"Bioinformatics","issn_l":"1367-4803","issn":["1367-4803","1367-4811"],"is_oa":false,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Bioinformatics","raw_type":"journal-article"},{"id":"pmid:32614380","is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/32614380","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Bioinformatics (Oxford, England)","raw_type":null},{"id":"pmh:oai:pubmedcentral.nih.gov:7824866","is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/7824866","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Bioinformatics","raw_type":"Text"}],"best_oa_location":{"id":"doi:10.1093/bioinformatics/btaa607","is_oa":true,"landing_page_url":"https://doi.org/10.1093/bioinformatics/btaa607","pdf_url":"https://academic.oup.com/bioinformatics/article-pdf/36/19/4860/36040368/btaa607.pdf","source":{"id":"https://openalex.org/S52395412","display_name":"Bioinformatics","issn_l":"1367-4803","issn":["1367-4803","1367-4811"],"is_oa":false,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Bioinformatics","raw_type":"journal-article"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/9","score":0.41999998688697815,"display_name":"Industry, innovation and infrastructure"}],"awards":[{"id":"https://openalex.org/G2787220474","display_name":null,"funder_award_id":"R01-GM122083","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"},{"id":"https://openalex.org/G556672640","display_name":null,"funder_award_id":"P50 AG-025688","funder_id":"https://openalex.org/F4320337337","funder_display_name":"National Institute on Aging"},{"id":"https://openalex.org/G6432502692","display_name":null,"funder_award_id":"R01GM124061","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"},{"id":"https://openalex.org/G953759355","display_name":null,"funder_award_id":"P20-GM103645","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"}],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337337","display_name":"National Institute on Aging","ror":"https://ror.org/049v75w11"},{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W3039872156.pdf","grobid_xml":"https://content.openalex.org/works/W3039872156.grobid-xml"},"referenced_works_count":36,"referenced_works":["https://openalex.org/W1813068103","https://openalex.org/W1967327758","https://openalex.org/W1979283544","https://openalex.org/W1981641790","https://openalex.org/W2023562174","https://openalex.org/W2067536340","https://openalex.org/W2069089843","https://openalex.org/W2097917417","https://openalex.org/W2098831884","https://openalex.org/W2102212449","https://openalex.org/W2110768394","https://openalex.org/W2112071941","https://openalex.org/W2128227827","https://openalex.org/W2140353888","https://openalex.org/W2152239989","https://openalex.org/W2155371242","https://openalex.org/W2161106528","https://openalex.org/W2190545194","https://openalex.org/W2286667211","https://openalex.org/W2511896561","https://openalex.org/W2555892463","https://openalex.org/W2578841491","https://openalex.org/W2598326928","https://openalex.org/W2792647356","https://openalex.org/W2801949492","https://openalex.org/W2885507494","https://openalex.org/W2911108160","https://openalex.org/W2949067670","https://openalex.org/W2949086748","https://openalex.org/W2950541340","https://openalex.org/W2951890476","https://openalex.org/W2954555796","https://openalex.org/W2962031564","https://openalex.org/W2973156019","https://openalex.org/W2998065416","https://openalex.org/W3000145506"],"related_works":["https://openalex.org/W4388813866","https://openalex.org/W1989415248","https://openalex.org/W2999104021","https://openalex.org/W977438452","https://openalex.org/W1481656249","https://openalex.org/W2850804095","https://openalex.org/W3041891053","https://openalex.org/W2162280767","https://openalex.org/W139386289","https://openalex.org/W4236933217"],"abstract_inverted_index":{"MOTIVATION:":[0],"Determining":[1],"the":[2,17,42,51,60,73,118,180,189],"sample":[3,34,87,121,183],"size":[4,35,88,122,184],"for":[5,20,33,37,90,158,178],"adequate":[6],"power":[7,84,108,119,142,150,156],"to":[8,82],"detect":[9],"statistical":[10],"significance":[11],"is":[12,57,197],"a":[13,25,79,98,107,112,147],"crucial":[14],"step":[15],"at":[16,204,212],"design":[18],"stage":[19],"high-throughput":[21],"experiments.":[22],"Even":[23],"though":[24],"number":[26],"of":[27,44,53,97,117,138,149],"methods":[28],"and":[29,39,70,86,106,115,120,154,185],"tools":[30],"are":[31,210],"available":[32,202,211],"calculation":[36],"microarray":[38],"RNA-seq":[40],"in":[41,50,65,127,134,144],"context":[43],"differential":[45],"expression":[46,104],"(DE),":[47],"this":[48],"topic":[49],"field":[52],"single-cell":[54,91],"RNA":[55],"sequencing":[56,186],"understudied.":[58],"Moreover,":[59],"unique":[61],"data":[62,99,125,209],"characteristics":[63,137],"present":[64],"scRNA-seq":[66],"such":[67],"as":[68],"sparsity":[69],"heterogeneity":[71],"increase":[72],"challenge.":[74],"RESULTS:":[75],"We":[76],"propose":[77],"POWSC,":[78],"simulation-based":[80],"method,":[81],"provide":[83],"evaluation":[85,114],"recommendation":[89],"RNA-sequencing":[92],"DE":[93],"analysis.":[94],"POWSC":[95,128,145,175,196],"consists":[96],"simulator":[100,126],"that":[101,110],"creates":[102],"realistic":[103],"data,":[105],"assessor":[109,143],"provides":[111,146],"comprehensive":[113],"visualization":[116],"relationship.":[123],"The":[124,141],"outperforms":[129],"two":[130,162],"other":[131],"state-of-art":[132],"simulators":[133],"capturing":[135],"key":[136],"real":[139],"datasets.":[140],"variety":[148],"evaluations":[151],"including":[152],"stratified":[153],"marginal":[155],"analyses":[157],"DEs":[159],"characterized":[160],"by":[161],"forms":[163],"(phase":[164],"transition":[165],"or":[166],"magnitude":[167],"tuning),":[168],"under":[169],"different":[170],"comparison":[171],"scenarios.":[172],"In":[173],"addition,":[174],"offers":[176],"information":[177],"optimizing":[179],"tradeoffs":[181],"between":[182],"depth":[187],"with":[188],"same":[190],"total":[191],"reads.":[192],"AVAILABILITY":[193],"AND":[194],"IMPLEMENTATION:":[195],"an":[198],"open-source":[199],"R":[200],"package":[201],"online":[203],"https://github.com/suke18/POWSC.":[205],"SUPPLEMENTARY":[206],"INFORMATION:":[207],"Supplementary":[208],"Bioinformatics":[213],"online.":[214]},"counts_by_year":[{"year":2026,"cited_by_count":4},{"year":2025,"cited_by_count":9},{"year":2024,"cited_by_count":9},{"year":2023,"cited_by_count":6},{"year":2022,"cited_by_count":4},{"year":2021,"cited_by_count":6}],"updated_date":"2026-08-01T09:00:35.917206","created_date":"2025-10-10T00:00:00"}