{"id":"https://openalex.org/W4406329164","doi":"https://doi.org/10.1093/bib/bbaf005","title":"A framework of multi-view machine learning for biological spectral unmixing of fluorophores with overlapping excitation and emission spectra","display_name":"A framework of multi-view machine learning for biological spectral unmixing of fluorophores with overlapping excitation and emission spectra","publication_year":2024,"publication_date":"2024-11-22","ids":{"openalex":"https://openalex.org/W4406329164","doi":"https://doi.org/10.1093/bib/bbaf005","pmid":"https://pubmed.ncbi.nlm.nih.gov/39804144"},"language":"en","primary_location":{"id":"doi:10.1093/bib/bbaf005","is_oa":true,"landing_page_url":"https://doi.org/10.1093/bib/bbaf005","pdf_url":"https://academic.oup.com/bib/article-pdf/26/1/bbaf005/61426017/bbaf005.pdf","source":{"id":"https://openalex.org/S91767247","display_name":"Briefings in Bioinformatics","issn_l":"1467-5463","issn":["1467-5463","1477-4054"],"is_oa":true,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Briefings in Bioinformatics","raw_type":"journal-article"},"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://academic.oup.com/bib/article-pdf/26/1/bbaf005/61426017/bbaf005.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5079985796","display_name":"Ruogu Wang","orcid":"https://orcid.org/0009-0004-5583-3541"},"institutions":[{"id":"https://openalex.org/I392282","display_name":"University at Albany, State University of New York","ror":"https://ror.org/012zs8222","country_code":"US","type":"education","lineage":["https://openalex.org/I392282"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Ruogu Wang","raw_affiliation_strings":["Department of Biology, University at Albany, SUNY , 1400 Washington Ave, Albany, NY 12222 ,","RNA Institute, University at Albany, SUNY , 1400 Washington Ave, Albany, NY 12222 ,"],"affiliations":[{"raw_affiliation_string":"Department of Biology, University at Albany, SUNY , 1400 Washington Ave, Albany, NY 12222 ,","institution_ids":["https://openalex.org/I392282"]},{"raw_affiliation_string":"RNA Institute, University at Albany, SUNY , 1400 Washington Ave, Albany, NY 12222 ,","institution_ids":["https://openalex.org/I392282"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101673055","display_name":"Yunlong Feng","orcid":"https://orcid.org/0000-0002-1519-2717"},"institutions":[{"id":"https://openalex.org/I392282","display_name":"University at Albany, State University of New York","ror":"https://ror.org/012zs8222","country_code":"US","type":"education","lineage":["https://openalex.org/I392282"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Yunlong Feng","raw_affiliation_strings":["Department of Mathematics and Statistics, University at Albany, SUNY , 1400 Washington Ave, Albany, NY 12222 ,"],"affiliations":[{"raw_affiliation_string":"Department of Mathematics and Statistics, University at Albany, SUNY , 1400 Washington Ave, Albany, NY 12222 ,","institution_ids":["https://openalex.org/I392282"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5076186001","display_name":"Alex M. Valm","orcid":"https://orcid.org/0000-0002-8286-080X"},"institutions":[{"id":"https://openalex.org/I392282","display_name":"University at Albany, State University of New York","ror":"https://ror.org/012zs8222","country_code":"US","type":"education","lineage":["https://openalex.org/I392282"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Alex M Valm","raw_affiliation_strings":["Department of Biology, University at Albany, SUNY , 1400 Washington Ave, Albany, NY 12222 ,","RNA Institute, University at Albany, SUNY , 1400 Washington Ave, Albany, NY 12222 ,"],"affiliations":[{"raw_affiliation_string":"Department of Biology, University at Albany, SUNY , 1400 Washington Ave, Albany, NY 12222 ,","institution_ids":["https://openalex.org/I392282"]},{"raw_affiliation_string":"RNA Institute, University at Albany, SUNY , 1400 Washington Ave, Albany, NY 12222 ,","institution_ids":["https://openalex.org/I392282"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5079985796"],"corresponding_institution_ids":["https://openalex.org/I392282"],"apc_list":{"value":4011,"currency":"USD","value_usd":4011},"apc_paid":{"value":4011,"currency":"USD","value_usd":4011},"fwci":1.5716,"has_fulltext":true,"cited_by_count":4,"citation_normalized_percentile":{"value":0.84704805,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":95,"max":99},"biblio":{"volume":"26","issue":"1","first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10977","display_name":"Optical Imaging and Spectroscopy Techniques","score":0.9932000041007996,"subfield":{"id":"https://openalex.org/subfields/2741","display_name":"Radiology, Nuclear Medicine and Imaging"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10977","display_name":"Optical Imaging and Spectroscopy Techniques","score":0.9932000041007996,"subfield":{"id":"https://openalex.org/subfields/2741","display_name":"Radiology, Nuclear Medicine and Imaging"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11324","display_name":"Spectroscopy Techniques in Biomedical and Chemical Research","score":0.9818000197410583,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10640","display_name":"Spectroscopy and Chemometric Analyses","score":0.9610000252723694,"subfield":{"id":"https://openalex.org/subfields/1602","display_name":"Analytical Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/excitation","display_name":"Excitation","score":0.7329756021499634},{"id":"https://openalex.org/keywords/spectral-line","display_name":"Spectral line","score":0.5883395075798035},{"id":"https://openalex.org/keywords/computer-science","display_name":"Computer science","score":0.46960458159446716},{"id":"https://openalex.org/keywords/emission-spectrum","display_name":"Emission spectrum","score":0.4513801336288452},{"id":"https://openalex.org/keywords/spectral-properties","display_name":"Spectral properties","score":0.42696595191955566},{"id":"https://openalex.org/keywords/artificial-intelligence","display_name":"Artificial intelligence","score":0.3982151448726654},{"id":"https://openalex.org/keywords/biological-system","display_name":"Biological system","score":0.3872876763343811},{"id":"https://openalex.org/keywords/physics","display_name":"Physics","score":0.24315577745437622},{"id":"https://openalex.org/keywords/biology","display_name":"Biology","score":0.13604959845542908},{"id":"https://openalex.org/keywords/astrophysics","display_name":"Astrophysics","score":0.08129671216011047}],"concepts":[{"id":"https://openalex.org/C83581075","wikidata":"https://www.wikidata.org/wiki/Q1361503","display_name":"Excitation","level":2,"score":0.7329756021499634},{"id":"https://openalex.org/C4839761","wikidata":"https://www.wikidata.org/wiki/Q212111","display_name":"Spectral line","level":2,"score":0.5883395075798035},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.46960458159446716},{"id":"https://openalex.org/C96141758","wikidata":"https://www.wikidata.org/wiki/Q216817","display_name":"Emission spectrum","level":3,"score":0.4513801336288452},{"id":"https://openalex.org/C2985906921","wikidata":"https://www.wikidata.org/wiki/Q190524","display_name":"Spectral properties","level":2,"score":0.42696595191955566},{"id":"https://openalex.org/C154945302","wikidata":"https://www.wikidata.org/wiki/Q11660","display_name":"Artificial intelligence","level":1,"score":0.3982151448726654},{"id":"https://openalex.org/C186060115","wikidata":"https://www.wikidata.org/wiki/Q30336093","display_name":"Biological system","level":1,"score":0.3872876763343811},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.24315577745437622},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.13604959845542908},{"id":"https://openalex.org/C44870925","wikidata":"https://www.wikidata.org/wiki/Q37547","display_name":"Astrophysics","level":1,"score":0.08129671216011047},{"id":"https://openalex.org/C1276947","wikidata":"https://www.wikidata.org/wiki/Q333","display_name":"Astronomy","level":1,"score":0.0},{"id":"https://openalex.org/C62520636","wikidata":"https://www.wikidata.org/wiki/Q944","display_name":"Quantum mechanics","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000069550","descriptor_name":"Machine Learning","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D000069550","descriptor_name":"Machine Learning","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D000069550","descriptor_name":"Machine Learning","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D000069550","descriptor_name":"Machine Learning","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D000069550","descriptor_name":"Machine Learning","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D000069550","descriptor_name":"Machine Learning","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D000069550","descriptor_name":"Machine Learning","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D000069550","descriptor_name":"Machine Learning","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D000465","descriptor_name":"Algorithms","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000465","descriptor_name":"Algorithms","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000465","descriptor_name":"Algorithms","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000465","descriptor_name":"Algorithms","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000465","descriptor_name":"Algorithms","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000465","descriptor_name":"Algorithms","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000465","descriptor_name":"Algorithms","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000465","descriptor_name":"Algorithms","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005456","descriptor_name":"Fluorescent Dyes","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D005456","descriptor_name":"Fluorescent Dyes","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D005456","descriptor_name":"Fluorescent Dyes","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D005456","descriptor_name":"Fluorescent Dyes","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D005456","descriptor_name":"Fluorescent Dyes","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D005456","descriptor_name":"Fluorescent Dyes","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D005456","descriptor_name":"Fluorescent Dyes","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D005456","descriptor_name":"Fluorescent Dyes","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D007091","descriptor_name":"Image Processing, Computer-Assisted","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D007091","descriptor_name":"Image Processing, Computer-Assisted","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D007091","descriptor_name":"Image Processing, Computer-Assisted","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D007091","descriptor_name":"Image Processing, Computer-Assisted","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D007091","descriptor_name":"Image Processing, Computer-Assisted","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D007091","descriptor_name":"Image Processing, Computer-Assisted","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D007091","descriptor_name":"Image Processing, Computer-Assisted","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D007091","descriptor_name":"Image Processing, Computer-Assisted","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D008856","descriptor_name":"Microscopy, Fluorescence","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D008856","descriptor_name":"Microscopy, Fluorescence","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D008856","descriptor_name":"Microscopy, Fluorescence","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D008856","descriptor_name":"Microscopy, Fluorescence","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D008856","descriptor_name":"Microscopy, Fluorescence","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D008856","descriptor_name":"Microscopy, Fluorescence","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D008856","descriptor_name":"Microscopy, Fluorescence","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D008856","descriptor_name":"Microscopy, Fluorescence","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D018613","descriptor_name":"Microscopy, Confocal","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018613","descriptor_name":"Microscopy, Confocal","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018613","descriptor_name":"Microscopy, Confocal","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018613","descriptor_name":"Microscopy, Confocal","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1093/bib/bbaf005","is_oa":true,"landing_page_url":"https://doi.org/10.1093/bib/bbaf005","pdf_url":"https://academic.oup.com/bib/article-pdf/26/1/bbaf005/61426017/bbaf005.pdf","source":{"id":"https://openalex.org/S91767247","display_name":"Briefings in Bioinformatics","issn_l":"1467-5463","issn":["1467-5463","1477-4054"],"is_oa":true,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Briefings in Bioinformatics","raw_type":"journal-article"},{"id":"pmid:39804144","is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/39804144","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Briefings in bioinformatics","raw_type":null},{"id":"pmh:oai:pubmedcentral.nih.gov:11726699","is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/11726699","pdf_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC11726699/pdf/bbaf005.pdf","source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Brief Bioinform","raw_type":"Text"}],"best_oa_location":{"id":"doi:10.1093/bib/bbaf005","is_oa":true,"landing_page_url":"https://doi.org/10.1093/bib/bbaf005","pdf_url":"https://academic.oup.com/bib/article-pdf/26/1/bbaf005/61426017/bbaf005.pdf","source":{"id":"https://openalex.org/S91767247","display_name":"Briefings in Bioinformatics","issn_l":"1467-5463","issn":["1467-5463","1477-4054"],"is_oa":true,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Briefings in Bioinformatics","raw_type":"journal-article"},"sustainable_development_goals":[],"awards":[{"id":"https://openalex.org/G224509593","display_name":null,"funder_award_id":"R01DE030927","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"},{"id":"https://openalex.org/G4301260759","display_name":null,"funder_award_id":"S10OD028600","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"},{"id":"https://openalex.org/G5372659370","display_name":null,"funder_award_id":"1636933","funder_id":"https://openalex.org/F4320306076","funder_display_name":"National Science Foundation"},{"id":"https://openalex.org/G6910641553","display_name":null,"funder_award_id":"1920920","funder_id":"https://openalex.org/F4320306076","funder_display_name":"National Science Foundation"},{"id":"https://openalex.org/G873944673","display_name":null,"funder_award_id":"2111080","funder_id":"https://openalex.org/F4320306076","funder_display_name":"National Science Foundation"},{"id":"https://openalex.org/G8796496170","display_name":null,"funder_award_id":"R01DE031213","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"}],"funders":[{"id":"https://openalex.org/F4320306076","display_name":"National Science Foundation","ror":"https://ror.org/021nxhr62"},{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W4406329164.pdf","grobid_xml":"https://content.openalex.org/works/W4406329164.grobid-xml"},"referenced_works_count":40,"referenced_works":["https://openalex.org/W93985499","https://openalex.org/W198705282","https://openalex.org/W1670132599","https://openalex.org/W1964570608","https://openalex.org/W2027878671","https://openalex.org/W2040325979","https://openalex.org/W2042590947","https://openalex.org/W2043079694","https://openalex.org/W2046033161","https://openalex.org/W2048679005","https://openalex.org/W2053830048","https://openalex.org/W2085789144","https://openalex.org/W2087537126","https://openalex.org/W2097581696","https://openalex.org/W2129625650","https://openalex.org/W2135029798","https://openalex.org/W2142674578","https://openalex.org/W2465777910","https://openalex.org/W2491458126","https://openalex.org/W2567080747","https://openalex.org/W2590019597","https://openalex.org/W2594777858","https://openalex.org/W2751272590","https://openalex.org/W2758611985","https://openalex.org/W2970563515","https://openalex.org/W2976589555","https://openalex.org/W3099180803","https://openalex.org/W3099850646","https://openalex.org/W4234514769","https://openalex.org/W4249992252","https://openalex.org/W4292363360","https://openalex.org/W4307279113","https://openalex.org/W4360939143","https://openalex.org/W4385525514","https://openalex.org/W4387837032","https://openalex.org/W4388423852","https://openalex.org/W6636883489","https://openalex.org/W6671578566","https://openalex.org/W6679376003","https://openalex.org/W6680012447"],"related_works":["https://openalex.org/W2364310969","https://openalex.org/W2373007135","https://openalex.org/W2390901883","https://openalex.org/W2012074534","https://openalex.org/W4211113703","https://openalex.org/W2086075532","https://openalex.org/W4399757072","https://openalex.org/W2502207150","https://openalex.org/W2952534122","https://openalex.org/W808924946"],"abstract_inverted_index":{"The":[0],"accuracy":[1,91],"of":[2,50,106,113,122,144,153,162,186,197,199,207],"assigning":[3],"fluorophore":[4,38,125],"identity":[5],"and":[6,84,147,159],"abundance,":[7],"known":[8],"as":[9],"spectral":[10,36,67,154,216],"unmixing,":[11],"in":[12,26,61,92,98,127,165,179,212,218],"biological":[13,220],"fluorescence":[14],"microscopy":[15],"images":[16,158,196],"remains":[17],"a":[18,47,74,99,133],"significant":[19],"challenge":[20],"due":[21],"to":[22,63,87],"the":[23,90,107,123,128,141,151,176,184,205,208],"substantial":[24],"overlap":[25,217],"emission":[27,44,66,85,104],"spectra":[28,45,60,86,105],"among":[29],"fluorophores.":[30],"In":[31,69],"traditional":[32],"laser":[33],"scanning":[34],"confocal":[35],"microscopy,":[37],"information":[39,146],"is":[40],"acquired":[41],"by":[42],"recording":[43,103],"with":[46,110,189,215],"single":[48,100],"combination":[49],"discrete":[51],"excitation":[52,59,83,114],"wavelengths.":[53],"However,":[54],"organic":[55],"fluorophores":[56,97,188,214],"possess":[57],"characteristic":[58],"addition":[62],"their":[64],"unique":[65],"signatures.":[68],"this":[70],"paper,":[71],"we":[72,116],"propose":[73,132],"generalized":[75],"multi-view":[76,134,210],"machine":[77,135],"learning":[78,136],"approach":[79],"that":[80,138],"leverages":[81],"both":[82],"significantly":[88],"improve":[89],"differentiating":[93],"multiple":[94,111],"highly":[95,190],"overlapping":[96,191],"image.":[101],"By":[102],"same":[108],"field":[109],"combinations":[112],"wavelengths,":[115],"obtain":[117],"data":[118,174],"representing":[119],"different":[120],"views":[121],"underlying":[124],"distribution":[126],"sample.":[129],"We":[130],"then":[131],"framework":[137],"allows":[139],"for":[140,183],"flexible":[142],"incorporation":[143],"noise":[145],"abundance":[148],"constraints,":[149],"enabling":[150],"extraction":[152],"signatures":[155],"from":[156],"reference":[157],"efficient":[160],"recovery":[161],"corresponding":[163],"abundances":[164],"unknown":[166],"mixed":[167],"images.":[168,221],"Numerical":[169],"experiments":[170],"on":[171,195],"simulated":[172],"image":[173],"demonstrate":[175],"method's":[177],"efficacy":[178],"improving":[180],"accuracy,":[181],"allowing":[182],"discrimination":[185],"100":[187],"spectra.":[192],"Furthermore,":[193],"validation":[194],"mixtures":[198],"fluorescently":[200],"labeled":[201],"Escherichia":[202],"coli":[203],"highlights":[204],"power":[206],"proposed":[209],"strategy":[211],"discriminating":[213],"real":[219]},"counts_by_year":[{"year":2026,"cited_by_count":2},{"year":2025,"cited_by_count":2}],"updated_date":"2026-04-14T08:04:32.555800","created_date":"2025-10-10T00:00:00"}