{"id":"https://openalex.org/W4389141292","doi":"https://doi.org/10.1093/bib/bbad425","title":"FireProt 2.0: web-based platform for the fully automated design of thermostable proteins","display_name":"FireProt 2.0: web-based platform for the fully automated design of thermostable proteins","publication_year":2023,"publication_date":"2023-11-22","ids":{"openalex":"https://openalex.org/W4389141292","doi":"https://doi.org/10.1093/bib/bbad425","pmid":"https://pubmed.ncbi.nlm.nih.gov/38018911"},"language":"en","primary_location":{"id":"doi:10.1093/bib/bbad425","is_oa":true,"landing_page_url":"https://doi.org/10.1093/bib/bbad425","pdf_url":"https://academic.oup.com/bib/article-pdf/25/1/bbad425/53896378/bbad425.pdf","source":{"id":"https://openalex.org/S91767247","display_name":"Briefings in Bioinformatics","issn_l":"1467-5463","issn":["1467-5463","1477-4054"],"is_oa":false,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Briefings in Bioinformatics","raw_type":"journal-article"},"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"https://academic.oup.com/bib/article-pdf/25/1/bbad425/53896378/bbad425.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5023686894","display_name":"Milo\u0161 Musil","orcid":"https://orcid.org/0000-0001-9373-7930"},"institutions":[{"id":"https://openalex.org/I21449261","display_name":"Masaryk University","ror":"https://ror.org/02j46qs45","country_code":"CZ","type":"education","lineage":["https://openalex.org/I21449261"]},{"id":"https://openalex.org/I4210102302","display_name":"University Hospital Brno","ror":"https://ror.org/00qq1fp34","country_code":"CZ","type":"healthcare","lineage":["https://openalex.org/I4210102302"]},{"id":"https://openalex.org/I4210113999","display_name":"RECETOX","ror":"https://ror.org/032hzqh22","country_code":"CZ","type":"facility","lineage":["https://openalex.org/I21449261","https://openalex.org/I4210113999"]},{"id":"https://openalex.org/I4210136987","display_name":"St. Anne's University Hospital Brno","ror":"https://ror.org/049bjee35","country_code":"CZ","type":"healthcare","lineage":["https://openalex.org/I4210136987"]},{"id":"https://openalex.org/I60587646","display_name":"Brno University of Technology","ror":"https://ror.org/03613d656","country_code":"CZ","type":"education","lineage":["https://openalex.org/I60587646"]}],"countries":["CZ"],"is_corresponding":true,"raw_author_name":"Milos Musil","raw_affiliation_strings":["Brno University of Technology , Faculty of Information Technology, , Brno , Czech Republic","Department of Information Systems , Faculty of Information Technology, , Brno , Czech Republic","International Clinical Research Centre, St. Anne\u2019s University Hospital Brno , Brno , Czech Republic","Loschmidt Laboratories , Department of Experimental Biology and RECETOX, , Brno , Czech Republic","Masaryk University , Department of Experimental Biology and RECETOX, , Brno , Czech Republic","Department of Information Systems, Faculty of Information Technology, Brno University of Technology, Brno, Czech Republic","International Clinical Research Centre, St. Anne's University Hospital Brno, Brno, Czech Republic","Loschmidt Laboratories, Department of Experimental Biology and RECETOX, Masaryk University, Brno, Czech Republic"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Brno University of Technology , Faculty of Information Technology, , Brno , Czech Republic","institution_ids":["https://openalex.org/I60587646"]},{"raw_affiliation_string":"Department of Information Systems , Faculty of Information Technology, , Brno , Czech Republic","institution_ids":["https://openalex.org/I60587646"]},{"raw_affiliation_string":"International Clinical Research Centre, St. Anne\u2019s University Hospital Brno , Brno , Czech Republic","institution_ids":["https://openalex.org/I4210136987","https://openalex.org/I4210102302"]},{"raw_affiliation_string":"Loschmidt Laboratories , Department of Experimental Biology and RECETOX, , Brno , Czech Republic","institution_ids":["https://openalex.org/I4210113999"]},{"raw_affiliation_string":"Masaryk University , Department of Experimental Biology and RECETOX, , Brno , Czech Republic","institution_ids":["https://openalex.org/I21449261"]},{"raw_affiliation_string":"Department of Information Systems, Faculty of Information Technology, Brno University of Technology, Brno, Czech Republic","institution_ids":["https://openalex.org/I60587646"]},{"raw_affiliation_string":"International Clinical Research Centre, St. Anne's University Hospital Brno, Brno, Czech Republic","institution_ids":["https://openalex.org/I4210136987","https://openalex.org/I4210102302"]},{"raw_affiliation_string":"Loschmidt Laboratories, Department of Experimental Biology and RECETOX, Masaryk University, Brno, Czech Republic","institution_ids":["https://openalex.org/I21449261"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5088954167","display_name":"Andrej Jezik","orcid":null},"institutions":[{"id":"https://openalex.org/I60587646","display_name":"Brno University of Technology","ror":"https://ror.org/03613d656","country_code":"CZ","type":"education","lineage":["https://openalex.org/I60587646"]}],"countries":["CZ"],"is_corresponding":false,"raw_author_name":"Andrej Jezik","raw_affiliation_strings":["Brno University of Technology , Faculty of Information Technology, , Brno , Czech Republic","Department of Information Systems , Faculty of Information Technology, , Brno , Czech Republic","Department of Information Systems, Faculty of Information Technology, Brno University of Technology, Brno, Czech Republic"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Brno University of Technology , Faculty of Information Technology, , Brno , Czech Republic","institution_ids":["https://openalex.org/I60587646"]},{"raw_affiliation_string":"Department of Information Systems , Faculty of Information Technology, , Brno , Czech Republic","institution_ids":["https://openalex.org/I60587646"]},{"raw_affiliation_string":"Department of Information Systems, Faculty of Information Technology, Brno University of Technology, Brno, Czech Republic","institution_ids":["https://openalex.org/I60587646"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5028843385","display_name":"Jana Horackova","orcid":"https://orcid.org/0000-0001-7119-121X"},"institutions":[{"id":"https://openalex.org/I21449261","display_name":"Masaryk University","ror":"https://ror.org/02j46qs45","country_code":"CZ","type":"education","lineage":["https://openalex.org/I21449261"]},{"id":"https://openalex.org/I4210113999","display_name":"RECETOX","ror":"https://ror.org/032hzqh22","country_code":"CZ","type":"facility","lineage":["https://openalex.org/I21449261","https://openalex.org/I4210113999"]}],"countries":["CZ"],"is_corresponding":false,"raw_author_name":"Jana Horackova","raw_affiliation_strings":["Loschmidt Laboratories , Department of Experimental Biology and RECETOX, , Brno , Czech Republic","Masaryk University , Department of Experimental Biology and RECETOX, , Brno , Czech Republic","Loschmidt Laboratories, Department of Experimental Biology and RECETOX, Masaryk University, Brno, Czech Republic"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Loschmidt Laboratories , Department of Experimental Biology and RECETOX, , Brno , Czech Republic","institution_ids":["https://openalex.org/I4210113999"]},{"raw_affiliation_string":"Masaryk University , Department of Experimental Biology and RECETOX, , Brno , Czech Republic","institution_ids":["https://openalex.org/I21449261"]},{"raw_affiliation_string":"Loschmidt Laboratories, Department of Experimental Biology and RECETOX, Masaryk University, Brno, Czech Republic","institution_ids":["https://openalex.org/I21449261"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5010754659","display_name":"Simeon Borko","orcid":"https://orcid.org/0009-0001-3122-810X"},"institutions":[{"id":"https://openalex.org/I21449261","display_name":"Masaryk University","ror":"https://ror.org/02j46qs45","country_code":"CZ","type":"education","lineage":["https://openalex.org/I21449261"]},{"id":"https://openalex.org/I4210102302","display_name":"University Hospital Brno","ror":"https://ror.org/00qq1fp34","country_code":"CZ","type":"healthcare","lineage":["https://openalex.org/I4210102302"]},{"id":"https://openalex.org/I4210113999","display_name":"RECETOX","ror":"https://ror.org/032hzqh22","country_code":"CZ","type":"facility","lineage":["https://openalex.org/I21449261","https://openalex.org/I4210113999"]},{"id":"https://openalex.org/I4210136987","display_name":"St. Anne's University Hospital Brno","ror":"https://ror.org/049bjee35","country_code":"CZ","type":"healthcare","lineage":["https://openalex.org/I4210136987"]},{"id":"https://openalex.org/I60587646","display_name":"Brno University of Technology","ror":"https://ror.org/03613d656","country_code":"CZ","type":"education","lineage":["https://openalex.org/I60587646"]}],"countries":["CZ"],"is_corresponding":false,"raw_author_name":"Simeon Borko","raw_affiliation_strings":["Brno University of Technology , Faculty of Information Technology, , Brno , Czech Republic","Department of Information Systems , Faculty of Information Technology, , Brno , Czech Republic","International Clinical Research Centre, St. Anne\u2019s University Hospital Brno , Brno , Czech Republic","Loschmidt Laboratories , Department of Experimental Biology and RECETOX, , Brno , Czech Republic","Masaryk University , Department of Experimental Biology and RECETOX, , Brno , Czech Republic","International Clinical Research Centre, St. Anne's University Hospital Brno, Brno, Czech Republic","Loschmidt Laboratories, Department of Experimental Biology and RECETOX, Masaryk University, Brno, Czech Republic","Department of Information Systems, Faculty of Information Technology, Brno University of Technology, Brno, Czech Republic"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Brno University of Technology , Faculty of Information Technology, , Brno , Czech Republic","institution_ids":["https://openalex.org/I60587646"]},{"raw_affiliation_string":"Department of Information Systems , Faculty of Information Technology, , Brno , Czech Republic","institution_ids":["https://openalex.org/I60587646"]},{"raw_affiliation_string":"International Clinical Research Centre, St. Anne\u2019s University Hospital Brno , Brno , Czech Republic","institution_ids":["https://openalex.org/I4210136987","https://openalex.org/I4210102302"]},{"raw_affiliation_string":"Loschmidt Laboratories , Department of Experimental Biology and RECETOX, , Brno , Czech Republic","institution_ids":["https://openalex.org/I4210113999"]},{"raw_affiliation_string":"Masaryk University , Department of Experimental Biology and RECETOX, , Brno , Czech Republic","institution_ids":["https://openalex.org/I21449261"]},{"raw_affiliation_string":"International Clinical Research Centre, St. Anne's University Hospital Brno, Brno, Czech Republic","institution_ids":["https://openalex.org/I4210136987","https://openalex.org/I4210102302"]},{"raw_affiliation_string":"Loschmidt Laboratories, Department of Experimental Biology and RECETOX, Masaryk University, Brno, Czech Republic","institution_ids":["https://openalex.org/I21449261"]},{"raw_affiliation_string":"Department of Information Systems, Faculty of Information Technology, Brno University of Technology, Brno, Czech Republic","institution_ids":["https://openalex.org/I60587646"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5093376201","display_name":"Petr Kabourek","orcid":null},"institutions":[{"id":"https://openalex.org/I21449261","display_name":"Masaryk University","ror":"https://ror.org/02j46qs45","country_code":"CZ","type":"education","lineage":["https://openalex.org/I21449261"]},{"id":"https://openalex.org/I4210102302","display_name":"University Hospital Brno","ror":"https://ror.org/00qq1fp34","country_code":"CZ","type":"healthcare","lineage":["https://openalex.org/I4210102302"]},{"id":"https://openalex.org/I4210113999","display_name":"RECETOX","ror":"https://ror.org/032hzqh22","country_code":"CZ","type":"facility","lineage":["https://openalex.org/I21449261","https://openalex.org/I4210113999"]},{"id":"https://openalex.org/I4210136987","display_name":"St. Anne's University Hospital Brno","ror":"https://ror.org/049bjee35","country_code":"CZ","type":"healthcare","lineage":["https://openalex.org/I4210136987"]}],"countries":["CZ"],"is_corresponding":false,"raw_author_name":"Petr Kabourek","raw_affiliation_strings":["International Clinical Research Centre, St. Anne\u2019s University Hospital Brno , Brno , Czech Republic","Loschmidt Laboratories , Department of Experimental Biology and RECETOX, , Brno , Czech Republic","Masaryk University , Department of Experimental Biology and RECETOX, , Brno , Czech Republic","Loschmidt Laboratories, Department of Experimental Biology and RECETOX, Masaryk University, Brno, Czech Republic","International Clinical Research Centre, St. Anne's University Hospital Brno, Brno, Czech Republic"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"International Clinical Research Centre, St. Anne\u2019s University Hospital Brno , Brno , Czech Republic","institution_ids":["https://openalex.org/I4210136987","https://openalex.org/I4210102302"]},{"raw_affiliation_string":"Loschmidt Laboratories , Department of Experimental Biology and RECETOX, , Brno , Czech Republic","institution_ids":["https://openalex.org/I4210113999"]},{"raw_affiliation_string":"Masaryk University , Department of Experimental Biology and RECETOX, , Brno , Czech Republic","institution_ids":["https://openalex.org/I21449261"]},{"raw_affiliation_string":"Loschmidt Laboratories, Department of Experimental Biology and RECETOX, Masaryk University, Brno, Czech Republic","institution_ids":["https://openalex.org/I21449261"]},{"raw_affiliation_string":"International Clinical Research Centre, St. Anne's University Hospital Brno, Brno, Czech Republic","institution_ids":["https://openalex.org/I4210136987","https://openalex.org/I4210102302"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5040585952","display_name":"Ji\u0159\u0131\u0301 Damborsk\u00fd","orcid":"https://orcid.org/0000-0002-7848-8216"},"institutions":[{"id":"https://openalex.org/I21449261","display_name":"Masaryk University","ror":"https://ror.org/02j46qs45","country_code":"CZ","type":"education","lineage":["https://openalex.org/I21449261"]},{"id":"https://openalex.org/I4210102302","display_name":"University Hospital Brno","ror":"https://ror.org/00qq1fp34","country_code":"CZ","type":"healthcare","lineage":["https://openalex.org/I4210102302"]},{"id":"https://openalex.org/I4210113999","display_name":"RECETOX","ror":"https://ror.org/032hzqh22","country_code":"CZ","type":"facility","lineage":["https://openalex.org/I21449261","https://openalex.org/I4210113999"]},{"id":"https://openalex.org/I4210136987","display_name":"St. Anne's University Hospital Brno","ror":"https://ror.org/049bjee35","country_code":"CZ","type":"healthcare","lineage":["https://openalex.org/I4210136987"]}],"countries":["CZ"],"is_corresponding":false,"raw_author_name":"Jiri Damborsky","raw_affiliation_strings":["International Clinical Research Centre, St. Anne\u2019s University Hospital Brno , Brno , Czech Republic","Loschmidt Laboratories , Department of Experimental Biology and RECETOX, , Brno , Czech Republic","Masaryk University , Department of Experimental Biology and RECETOX, , Brno , Czech Republic","International Clinical Research Centre, St. Anne's University Hospital Brno, Brno, Czech Republic","Loschmidt Laboratories, Department of Experimental Biology and RECETOX, Masaryk University, Brno, Czech Republic"],"raw_orcid":"https://orcid.org/0000-0002-7848-8216","affiliations":[{"raw_affiliation_string":"International Clinical Research Centre, St. Anne\u2019s University Hospital Brno , Brno , Czech Republic","institution_ids":["https://openalex.org/I4210136987","https://openalex.org/I4210102302"]},{"raw_affiliation_string":"Loschmidt Laboratories , Department of Experimental Biology and RECETOX, , Brno , Czech Republic","institution_ids":["https://openalex.org/I4210113999"]},{"raw_affiliation_string":"Masaryk University , Department of Experimental Biology and RECETOX, , Brno , Czech Republic","institution_ids":["https://openalex.org/I21449261"]},{"raw_affiliation_string":"International Clinical Research Centre, St. Anne's University Hospital Brno, Brno, Czech Republic","institution_ids":["https://openalex.org/I4210136987","https://openalex.org/I4210102302"]},{"raw_affiliation_string":"Loschmidt Laboratories, Department of Experimental Biology and RECETOX, Masaryk University, Brno, Czech Republic","institution_ids":["https://openalex.org/I21449261"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5003693498","display_name":"David Bedn\u00e1\u0159","orcid":"https://orcid.org/0000-0002-6803-0340"},"institutions":[{"id":"https://openalex.org/I21449261","display_name":"Masaryk University","ror":"https://ror.org/02j46qs45","country_code":"CZ","type":"education","lineage":["https://openalex.org/I21449261"]},{"id":"https://openalex.org/I4210102302","display_name":"University Hospital Brno","ror":"https://ror.org/00qq1fp34","country_code":"CZ","type":"healthcare","lineage":["https://openalex.org/I4210102302"]},{"id":"https://openalex.org/I4210113999","display_name":"RECETOX","ror":"https://ror.org/032hzqh22","country_code":"CZ","type":"facility","lineage":["https://openalex.org/I21449261","https://openalex.org/I4210113999"]},{"id":"https://openalex.org/I4210136987","display_name":"St. Anne's University Hospital Brno","ror":"https://ror.org/049bjee35","country_code":"CZ","type":"healthcare","lineage":["https://openalex.org/I4210136987"]}],"countries":["CZ"],"is_corresponding":true,"raw_author_name":"David Bednar","raw_affiliation_strings":["International Clinical Research Centre, St. Anne\u2019s University Hospital Brno , Brno , Czech Republic","Loschmidt Laboratories , Department of Experimental Biology and RECETOX, , Brno , Czech Republic","Masaryk University , Department of Experimental Biology and RECETOX, , Brno , Czech Republic","Loschmidt Laboratories, Department of Experimental Biology and RECETOX, Masaryk University, Brno, Czech Republic","International Clinical Research Centre, St. Anne's University Hospital Brno, Brno, Czech Republic"],"raw_orcid":"https://orcid.org/0000-0002-6803-0340","affiliations":[{"raw_affiliation_string":"International Clinical Research Centre, St. Anne\u2019s University Hospital Brno , Brno , Czech Republic","institution_ids":["https://openalex.org/I4210136987","https://openalex.org/I4210102302"]},{"raw_affiliation_string":"Loschmidt Laboratories , Department of Experimental Biology and RECETOX, , Brno , Czech Republic","institution_ids":["https://openalex.org/I4210113999"]},{"raw_affiliation_string":"Masaryk University , Department of Experimental Biology and RECETOX, , Brno , Czech Republic","institution_ids":["https://openalex.org/I21449261"]},{"raw_affiliation_string":"Loschmidt Laboratories, Department of Experimental Biology and RECETOX, Masaryk University, Brno, Czech Republic","institution_ids":["https://openalex.org/I21449261"]},{"raw_affiliation_string":"International Clinical Research Centre, St. Anne's University Hospital Brno, Brno, Czech Republic","institution_ids":["https://openalex.org/I4210136987","https://openalex.org/I4210102302"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":7,"corresponding_author_ids":["https://openalex.org/A5003693498","https://openalex.org/A5023686894"],"corresponding_institution_ids":["https://openalex.org/I21449261","https://openalex.org/I4210102302","https://openalex.org/I4210113999","https://openalex.org/I4210136987","https://openalex.org/I60587646"],"apc_list":{"value":4011,"currency":"USD","value_usd":4011},"apc_paid":{"value":4011,"currency":"USD","value_usd":4011},"fwci":13.7433,"has_fulltext":true,"cited_by_count":93,"citation_normalized_percentile":{"value":0.99289779,"is_in_top_1_percent":true,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":90,"max":100},"biblio":{"volume":"25","issue":"1","first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10015","display_name":"Genomics and Phylogenetic Studies","score":0.9944999814033508,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10015","display_name":"Genomics and Phylogenetic Studies","score":0.9944999814033508,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":0.9883000254631042,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.987500011920929,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/saturated-mutagenesis","display_name":"Saturated mutagenesis","score":0.7682747840881348},{"id":"https://openalex.org/keywords/computer-science","display_name":"Computer science","score":0.6310444474220276},{"id":"https://openalex.org/keywords/point-mutation","display_name":"Point mutation","score":0.6104099154472351},{"id":"https://openalex.org/keywords/mutant","display_name":"Mutant","score":0.532757043838501},{"id":"https://openalex.org/keywords/computational-biology","display_name":"Computational biology","score":0.5247778296470642},{"id":"https://openalex.org/keywords/protein-design","display_name":"Protein design","score":0.4962049126625061},{"id":"https://openalex.org/keywords/mutagenesis","display_name":"Mutagenesis","score":0.4684242010116577},{"id":"https://openalex.org/keywords/set","display_name":"Set (abstract data type)","score":0.4498599171638489},{"id":"https://openalex.org/keywords/protein-engineering","display_name":"Protein engineering","score":0.43559974431991577},{"id":"https://openalex.org/keywords/homology-modeling","display_name":"Homology modeling","score":0.41155633330345154},{"id":"https://openalex.org/keywords/stability","display_name":"Stability (learning theory)","score":0.4107433557510376},{"id":"https://openalex.org/keywords/mutation","display_name":"Mutation","score":0.410243958234787},{"id":"https://openalex.org/keywords/protein-structure","display_name":"Protein structure","score":0.37892043590545654},{"id":"https://openalex.org/keywords/biology","display_name":"Biology","score":0.3388761878013611},{"id":"https://openalex.org/keywords/genetics","display_name":"Genetics","score":0.3196741044521332},{"id":"https://openalex.org/keywords/programming-language","display_name":"Programming language","score":0.1449085772037506},{"id":"https://openalex.org/keywords/machine-learning","display_name":"Machine learning","score":0.13573452830314636}],"concepts":[{"id":"https://openalex.org/C109129352","wikidata":"https://www.wikidata.org/wiki/Q7426583","display_name":"Saturated mutagenesis","level":4,"score":0.7682747840881348},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.6310444474220276},{"id":"https://openalex.org/C176944494","wikidata":"https://www.wikidata.org/wiki/Q1415380","display_name":"Point mutation","level":4,"score":0.6104099154472351},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.532757043838501},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.5247778296470642},{"id":"https://openalex.org/C152769699","wikidata":"https://www.wikidata.org/wiki/Q410814","display_name":"Protein design","level":3,"score":0.4962049126625061},{"id":"https://openalex.org/C16318435","wikidata":"https://www.wikidata.org/wiki/Q149299","display_name":"Mutagenesis","level":4,"score":0.4684242010116577},{"id":"https://openalex.org/C177264268","wikidata":"https://www.wikidata.org/wiki/Q1514741","display_name":"Set (abstract data type)","level":2,"score":0.4498599171638489},{"id":"https://openalex.org/C147816474","wikidata":"https://www.wikidata.org/wiki/Q169525","display_name":"Protein engineering","level":3,"score":0.43559974431991577},{"id":"https://openalex.org/C169627665","wikidata":"https://www.wikidata.org/wiki/Q5767913","display_name":"Homology modeling","level":3,"score":0.41155633330345154},{"id":"https://openalex.org/C112972136","wikidata":"https://www.wikidata.org/wiki/Q7595718","display_name":"Stability (learning theory)","level":2,"score":0.4107433557510376},{"id":"https://openalex.org/C501734568","wikidata":"https://www.wikidata.org/wiki/Q42918","display_name":"Mutation","level":3,"score":0.410243958234787},{"id":"https://openalex.org/C47701112","wikidata":"https://www.wikidata.org/wiki/Q735188","display_name":"Protein structure","level":2,"score":0.37892043590545654},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3388761878013611},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.3196741044521332},{"id":"https://openalex.org/C199360897","wikidata":"https://www.wikidata.org/wiki/Q9143","display_name":"Programming language","level":1,"score":0.1449085772037506},{"id":"https://openalex.org/C119857082","wikidata":"https://www.wikidata.org/wiki/Q2539","display_name":"Machine learning","level":1,"score":0.13573452830314636},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.0},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000465","descriptor_name":"Algorithms","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D000465","descriptor_name":"Algorithms","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D000465","descriptor_name":"Algorithms","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D000465","descriptor_name":"Algorithms","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D009154","descriptor_name":"Mutation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009154","descriptor_name":"Mutation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009154","descriptor_name":"Mutation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009154","descriptor_name":"Mutation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011506","descriptor_name":"Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D011506","descriptor_name":"Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D011506","descriptor_name":"Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D011506","descriptor_name":"Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D011506","descriptor_name":"Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D011506","descriptor_name":"Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D011506","descriptor_name":"Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D011506","descriptor_name":"Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D020407","descriptor_name":"Internet","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D020407","descriptor_name":"Internet","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D020407","descriptor_name":"Internet","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D020407","descriptor_name":"Internet","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D055550","descriptor_name":"Protein Stability","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D055550","descriptor_name":"Protein Stability","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D055550","descriptor_name":"Protein Stability","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D055550","descriptor_name":"Protein Stability","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":4,"locations":[{"id":"doi:10.1093/bib/bbad425","is_oa":true,"landing_page_url":"https://doi.org/10.1093/bib/bbad425","pdf_url":"https://academic.oup.com/bib/article-pdf/25/1/bbad425/53896378/bbad425.pdf","source":{"id":"https://openalex.org/S91767247","display_name":"Briefings in Bioinformatics","issn_l":"1467-5463","issn":["1467-5463","1477-4054"],"is_oa":false,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Briefings in Bioinformatics","raw_type":"journal-article"},{"id":"pmid:38018911","is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/38018911","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Briefings in bioinformatics","raw_type":null},{"id":"pmh:oai:is.muni.cz:2349701","is_oa":true,"landing_page_url":"https://is.muni.cz/publication/2349701","pdf_url":"https://is.muni.cz/publication/2349701/2349701.pdf","source":{"id":"https://openalex.org/S4306400102","display_name":"Ve\u0159ejn\u00e9 slu\u017eby Informa\u010dn\u00edho syst\u00e9mu (Masarykiana Brunensis Universitas)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Briefings in Bioinformatics","raw_type":"info:eu-repo/semantics/article"},{"id":"pmh:oai:pubmedcentral.nih.gov:10685400","is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/10685400","pdf_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC10685400/pdf/bbad425.pdf","source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Brief Bioinform","raw_type":"Text"}],"best_oa_location":{"id":"doi:10.1093/bib/bbad425","is_oa":true,"landing_page_url":"https://doi.org/10.1093/bib/bbad425","pdf_url":"https://academic.oup.com/bib/article-pdf/25/1/bbad425/53896378/bbad425.pdf","source":{"id":"https://openalex.org/S91767247","display_name":"Briefings in Bioinformatics","issn_l":"1467-5463","issn":["1467-5463","1477-4054"],"is_oa":false,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Briefings in Bioinformatics","raw_type":"journal-article"},"sustainable_development_goals":[],"awards":[{"id":"https://openalex.org/G2351268093","display_name":null,"funder_award_id":"857560","funder_id":"https://openalex.org/F4320320300","funder_display_name":"European Commission"},{"id":"https://openalex.org/G252385833","display_name":null,"funder_award_id":"LM2023069","funder_id":"https://openalex.org/F4320336687","funder_display_name":"RECETOX P\u0159\u00edrodov\u011bdeck\u00e9 Fakulty Masarykovy Univerzity"},{"id":"https://openalex.org/G2706813561","display_name":null,"funder_award_id":"CZ.02.1.01/0.0/0.0/17_043/0009632","funder_id":"https://openalex.org/F4320336687","funder_display_name":"RECETOX P\u0159\u00edrodov\u011bdeck\u00e9 Fakulty Masarykovy Univerzity"},{"id":"https://openalex.org/G300686824","display_name":null,"funder_award_id":"FIT-S-23-8209","funder_id":"https://openalex.org/F4320322748","funder_display_name":"Vysok\u00e9 U\u010den\u00ed Technick\u00e9 v Brn\u011b"},{"id":"https://openalex.org/G3622461675","display_name":null,"funder_award_id":"CZ.02.1.01","funder_id":"https://openalex.org/F4320320300","funder_display_name":"European Commission"},{"id":"https://openalex.org/G4158262239","display_name":null,"funder_award_id":"ELIXIR LM2023055","funder_id":"https://openalex.org/F4320336687","funder_display_name":"RECETOX P\u0159\u00edrodov\u011bdeck\u00e9 Fakulty Masarykovy Univerzity"},{"id":"https://openalex.org/G4513346166","display_name":null,"funder_award_id":"FW03010208","funder_id":"https://openalex.org/F4320315323","funder_display_name":"Technology Agency of the Czech Republic"},{"id":"https://openalex.org/G5535269418","display_name":null,"funder_award_id":"LX22NPO5107","funder_id":"https://openalex.org/F4320320300","funder_display_name":"European Commission"},{"id":"https://openalex.org/G5566694801","display_name":null,"funder_award_id":"CZ.02.1.01/0.0/0.0/17_043/0009632","funder_id":"https://openalex.org/F4320320300","funder_display_name":"European Commission"},{"id":"https://openalex.org/G6806075293","display_name":null,"funder_award_id":"TN02000122","funder_id":"https://openalex.org/F4320315323","funder_display_name":"Technology Agency of the Czech Republic"},{"id":"https://openalex.org/G7545815938","display_name":null,"funder_award_id":"LM2018140","funder_id":"https://openalex.org/F4320320300","funder_display_name":"European Commission"},{"id":"https://openalex.org/G8462350515","display_name":null,"funder_award_id":"LM2018140","funder_id":"https://openalex.org/F4320322748","funder_display_name":"Vysok\u00e9 U\u010den\u00ed Technick\u00e9 v Brn\u011b"},{"id":"https://openalex.org/G8844530579","display_name":null,"funder_award_id":"RI LM2023069","funder_id":"https://openalex.org/F4320336687","funder_display_name":"RECETOX P\u0159\u00edrodov\u011bdeck\u00e9 Fakulty Masarykovy Univerzity"}],"funders":[{"id":"https://openalex.org/F4320315323","display_name":"Technology Agency of the Czech Republic","ror":null},{"id":"https://openalex.org/F4320320300","display_name":"European Commission","ror":"https://ror.org/00k4n6c32"},{"id":"https://openalex.org/F4320322748","display_name":"Vysok\u00e9 U\u010den\u00ed Technick\u00e9 v Brn\u011b","ror":"https://ror.org/03613d656"},{"id":"https://openalex.org/F4320336687","display_name":"RECETOX P\u0159\u00edrodov\u011bdeck\u00e9 Fakulty Masarykovy Univerzity","ror":null}],"has_content":{"grobid_xml":false,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W4389141292.pdf"},"referenced_works_count":110,"referenced_works":["https://openalex.org/W980708197","https://openalex.org/W1963697504","https://openalex.org/W1967209541","https://openalex.org/W1967293793","https://openalex.org/W1977832025","https://openalex.org/W1979762151","https://openalex.org/W1985006546","https://openalex.org/W1997064329","https://openalex.org/W2003810144","https://openalex.org/W2005767394","https://openalex.org/W2021769301","https://openalex.org/W2023346730","https://openalex.org/W2023490488","https://openalex.org/W2029679481","https://openalex.org/W2032479422","https://openalex.org/W2034557959","https://openalex.org/W2041476324","https://openalex.org/W2042848740","https://openalex.org/W2047289990","https://openalex.org/W2048765448","https://openalex.org/W2056053449","https://openalex.org/W2057007659","https://openalex.org/W2064488723","https://openalex.org/W2069319961","https://openalex.org/W2070537581","https://openalex.org/W2074002527","https://openalex.org/W2077564233","https://openalex.org/W2087300028","https://openalex.org/W2092006899","https://openalex.org/W2097980499","https://openalex.org/W2101414350","https://openalex.org/W2102461176","https://openalex.org/W2104418738","https://openalex.org/W2106612397","https://openalex.org/W2110068022","https://openalex.org/W2112919985","https://openalex.org/W2114107787","https://openalex.org/W2116007667","https://openalex.org/W2117747873","https://openalex.org/W2120949690","https://openalex.org/W2121519777","https://openalex.org/W2123687265","https://openalex.org/W2123906667","https://openalex.org/W2124351063","https://openalex.org/W2127322768","https://openalex.org/W2132540199","https://openalex.org/W2142678478","https://openalex.org/W2148853951","https://openalex.org/W2151529283","https://openalex.org/W2153457180","https://openalex.org/W2155458838","https://openalex.org/W2161690185","https://openalex.org/W2171641243","https://openalex.org/W2171901881","https://openalex.org/W2198618766","https://openalex.org/W2207955902","https://openalex.org/W2262896633","https://openalex.org/W2298175344","https://openalex.org/W2298358682","https://openalex.org/W2307896283","https://openalex.org/W2324374958","https://openalex.org/W2412714128","https://openalex.org/W2428593791","https://openalex.org/W2460331810","https://openalex.org/W2473135273","https://openalex.org/W2606439133","https://openalex.org/W2607570365","https://openalex.org/W2615841538","https://openalex.org/W2710675085","https://openalex.org/W2734861826","https://openalex.org/W2741292700","https://openalex.org/W2743493305","https://openalex.org/W2783931353","https://openalex.org/W2788217325","https://openalex.org/W2799513065","https://openalex.org/W2896607521","https://openalex.org/W2902559445","https://openalex.org/W2903887467","https://openalex.org/W2911872747","https://openalex.org/W2912783011","https://openalex.org/W2940006595","https://openalex.org/W2949291356","https://openalex.org/W2953906682","https://openalex.org/W2954528946","https://openalex.org/W2982678525","https://openalex.org/W2987356792","https://openalex.org/W3010364596","https://openalex.org/W3083008290","https://openalex.org/W3086012725","https://openalex.org/W3104081477","https://openalex.org/W3105751341","https://openalex.org/W3113826677","https://openalex.org/W3115284089","https://openalex.org/W3123997054","https://openalex.org/W3148270819","https://openalex.org/W3153187427","https://openalex.org/W3156303331","https://openalex.org/W3159719254","https://openalex.org/W3211795435","https://openalex.org/W4213344517","https://openalex.org/W4233120011","https://openalex.org/W4296032638","https://openalex.org/W4312198153","https://openalex.org/W4319296017","https://openalex.org/W4366392978","https://openalex.org/W4376612841","https://openalex.org/W4379600237","https://openalex.org/W6739886451","https://openalex.org/W6770332402","https://openalex.org/W6788000285"],"related_works":["https://openalex.org/W2368917022","https://openalex.org/W2084262300","https://openalex.org/W1964597525","https://openalex.org/W2034928589","https://openalex.org/W2913781108","https://openalex.org/W2610280852","https://openalex.org/W2561974534","https://openalex.org/W4313610832","https://openalex.org/W2036693159","https://openalex.org/W2149291385"],"abstract_inverted_index":{"Thermostable":[0],"proteins":[1,17],"find":[2],"their":[3],"use":[4],"in":[5,20],"numerous":[6],"biomedical":[7],"and":[8,77,91,162],"biotechnological":[9],"applications.":[10],"However,":[11,30],"the":[12,31,42,53,68,74,88,92,109,113,117,124,138,169],"computational":[13,55],"design":[14,56],"of":[15,33,44,57,67,130,137],"stable":[16,34,59],"often":[18],"results":[19],"single-point":[21],"mutations":[22,144],"with":[23,80,175],"a":[24,102,128,134,163],"limited":[25],"effect":[26,115],"on":[27,65,146],"protein":[28,140],"stability.":[29],"construction":[32],"multiple-point":[35,60],"mutants":[36],"can":[37,121],"prove":[38],"difficult":[39],"due":[40],"to":[41,127,173],"possibility":[43],"antagonistic":[45,114],"effects":[46],"between":[47,116],"individual":[48,118],"mutations.":[49,119],"FireProt":[50,62,71,85,125,157,178],"protocol":[51],"enables":[52],"automated":[54],"highly":[58],"mutants.":[61],"2.0":[63,86,158,179],"builds":[64],"top":[66],"previously":[69],"published":[70],"web,":[72],"retaining":[73],"original":[75],"functionality":[76],"expanding":[78],"it":[79],"several":[81],"new":[82],"stabilization":[83],"strategies.":[84],"integrates":[87],"AlphaFold":[89],"database":[90],"homology":[93],"modeling":[94],"for":[95],"structure":[96],"prediction,":[97],"enabling":[98],"calculations":[99],"starting":[100],"from":[101],"sequence.":[103],"Multiple-point":[104],"designs":[105],"are":[106],"constructed":[107],"using":[108],"Bron-Kerbosch":[110],"algorithm":[111],"minimizing":[112],"Users":[120],"newly":[122],"limit":[123],"calculation":[126],"set":[129],"user-defined":[131],"mutations,":[132],"run":[133],"saturation":[135],"mutagenesis":[136],"whole":[139],"or":[141],"select":[142],"rigidifying":[143],"based":[145],"B-factors.":[147],"Evolution-based":[148],"back-to-consensus":[149],"strategy":[150],"is":[151,159,180],"complemented":[152],"by":[153],"ancestral":[154],"sequence":[155],"reconstruction.":[156],"significantly":[160],"faster":[161],"reworked":[164],"graphical":[165],"user":[166],"interface":[167],"broadens":[168],"tool's":[170],"availability":[171],"even":[172],"users":[174],"older":[176],"hardware.":[177],"freely":[181],"available":[182],"at":[183],"http://loschmidt.chemi.muni.cz/fireprotweb.":[184]},"counts_by_year":[{"year":2026,"cited_by_count":24},{"year":2025,"cited_by_count":48},{"year":2024,"cited_by_count":20},{"year":2023,"cited_by_count":1}],"updated_date":"2026-05-08T15:41:06.802602","created_date":"2025-10-10T00:00:00"}