{"id":"https://openalex.org/W4225666561","doi":"https://doi.org/10.1093/bib/bbac183","title":"ExsgRNA: reduce off-target efficiency by on-target mismatched sgRNA","display_name":"ExsgRNA: reduce off-target efficiency by on-target mismatched sgRNA","publication_year":2022,"publication_date":"2022-04-26","ids":{"openalex":"https://openalex.org/W4225666561","doi":"https://doi.org/10.1093/bib/bbac183","pmid":"https://pubmed.ncbi.nlm.nih.gov/35580855"},"language":"en","primary_location":{"id":"doi:10.1093/bib/bbac183","is_oa":false,"landing_page_url":"https://doi.org/10.1093/bib/bbac183","pdf_url":null,"source":{"id":"https://openalex.org/S91767247","display_name":"Briefings in Bioinformatics","issn_l":"1467-5463","issn":["1467-5463","1477-4054"],"is_oa":false,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Briefings in Bioinformatics","raw_type":"journal-article"},"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5101554993","display_name":"Wei\u2010Xin Hu","orcid":null},"institutions":[{"id":"https://openalex.org/I87445476","display_name":"Xi'an Jiaotong University","ror":"https://ror.org/017zhmm22","country_code":"CN","type":"education","lineage":["https://openalex.org/I87445476"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Wei-Xin Hu","raw_affiliation_strings":["Biomedical Informatics & Genomics Center, Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi\u2019an Jiaotong University , Xi\u2019an 710049, P.R. China"],"affiliations":[{"raw_affiliation_string":"Biomedical Informatics & Genomics Center, Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi\u2019an Jiaotong University , Xi\u2019an 710049, P.R. China","institution_ids":["https://openalex.org/I87445476"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101480907","display_name":"Yu Rong","orcid":"https://orcid.org/0000-0003-4136-5551"},"institutions":[{"id":"https://openalex.org/I87445476","display_name":"Xi'an Jiaotong University","ror":"https://ror.org/017zhmm22","country_code":"CN","type":"education","lineage":["https://openalex.org/I87445476"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Yu Rong","raw_affiliation_strings":["Biomedical Informatics & Genomics Center, Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi\u2019an Jiaotong University , Xi\u2019an 710049, P.R. China"],"affiliations":[{"raw_affiliation_string":"Biomedical Informatics & Genomics Center, Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi\u2019an Jiaotong University , Xi\u2019an 710049, P.R. China","institution_ids":["https://openalex.org/I87445476"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5059564497","display_name":"Yan Guo","orcid":"https://orcid.org/0000-0002-7364-2392"},"institutions":[{"id":"https://openalex.org/I87445476","display_name":"Xi'an Jiaotong University","ror":"https://ror.org/017zhmm22","country_code":"CN","type":"education","lineage":["https://openalex.org/I87445476"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Yan Guo","raw_affiliation_strings":["Biomedical Informatics & Genomics Center, Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi\u2019an Jiaotong University , Xi\u2019an 710049, P.R. China"],"affiliations":[{"raw_affiliation_string":"Biomedical Informatics & Genomics Center, Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi\u2019an Jiaotong University , Xi\u2019an 710049, P.R. China","institution_ids":["https://openalex.org/I87445476"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5042080483","display_name":"Feng Jiang","orcid":"https://orcid.org/0000-0002-9726-5720"},"institutions":[{"id":"https://openalex.org/I87445476","display_name":"Xi'an Jiaotong University","ror":"https://ror.org/017zhmm22","country_code":"CN","type":"education","lineage":["https://openalex.org/I87445476"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Feng Jiang","raw_affiliation_strings":["Biomedical Informatics & Genomics Center, Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi\u2019an Jiaotong University , Xi\u2019an 710049, P.R. China"],"affiliations":[{"raw_affiliation_string":"Biomedical Informatics & Genomics Center, Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi\u2019an Jiaotong University , Xi\u2019an 710049, P.R. China","institution_ids":["https://openalex.org/I87445476"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101787397","display_name":"Wen Tian","orcid":"https://orcid.org/0000-0003-2418-5209"},"institutions":[{"id":"https://openalex.org/I87445476","display_name":"Xi'an Jiaotong University","ror":"https://ror.org/017zhmm22","country_code":"CN","type":"education","lineage":["https://openalex.org/I87445476"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Wen Tian","raw_affiliation_strings":["Biomedical Informatics & Genomics Center, Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi\u2019an Jiaotong University , Xi\u2019an 710049, P.R. China"],"affiliations":[{"raw_affiliation_string":"Biomedical Informatics & Genomics Center, Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi\u2019an Jiaotong University , Xi\u2019an 710049, P.R. China","institution_ids":["https://openalex.org/I87445476"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100353574","display_name":"Hao Chen","orcid":"https://orcid.org/0000-0002-6408-9505"},"institutions":[{"id":"https://openalex.org/I87445476","display_name":"Xi'an Jiaotong University","ror":"https://ror.org/017zhmm22","country_code":"CN","type":"education","lineage":["https://openalex.org/I87445476"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Hao Chen","raw_affiliation_strings":["Biomedical Informatics & Genomics Center, Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi\u2019an Jiaotong University , Xi\u2019an 710049, P.R. China"],"affiliations":[{"raw_affiliation_string":"Biomedical Informatics & Genomics Center, Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi\u2019an Jiaotong University , Xi\u2019an 710049, P.R. China","institution_ids":["https://openalex.org/I87445476"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5041151811","display_name":"Shan\u2010Shan Dong","orcid":"https://orcid.org/0000-0001-6976-4576"},"institutions":[{"id":"https://openalex.org/I87445476","display_name":"Xi'an Jiaotong University","ror":"https://ror.org/017zhmm22","country_code":"CN","type":"education","lineage":["https://openalex.org/I87445476"]}],"countries":["CN"],"is_corresponding":true,"raw_author_name":"Shan-Shan Dong","raw_affiliation_strings":["Biomedical Informatics & Genomics Center, Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi\u2019an Jiaotong University , Xi\u2019an 710049, P.R. China","Research Institute of Xi\u2019an Jiaotong University , Zhejiang, China"],"affiliations":[{"raw_affiliation_string":"Biomedical Informatics & Genomics Center, Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi\u2019an Jiaotong University , Xi\u2019an 710049, P.R. China","institution_ids":["https://openalex.org/I87445476"]},{"raw_affiliation_string":"Research Institute of Xi\u2019an Jiaotong University , Zhejiang, China","institution_ids":["https://openalex.org/I87445476"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5078356696","display_name":"Tie\u2010Lin Yang","orcid":"https://orcid.org/0000-0001-7062-3025"},"institutions":[{"id":"https://openalex.org/I87445476","display_name":"Xi'an Jiaotong University","ror":"https://ror.org/017zhmm22","country_code":"CN","type":"education","lineage":["https://openalex.org/I87445476"]}],"countries":["CN"],"is_corresponding":true,"raw_author_name":"Tie-Lin Yang","raw_affiliation_strings":["Biomedical Informatics & Genomics Center, Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi\u2019an Jiaotong University , Xi\u2019an 710049, P.R. China"],"affiliations":[{"raw_affiliation_string":"Biomedical Informatics & Genomics Center, Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi\u2019an Jiaotong University , Xi\u2019an 710049, P.R. China","institution_ids":["https://openalex.org/I87445476"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":8,"corresponding_author_ids":["https://openalex.org/A5041151811","https://openalex.org/A5078356696"],"corresponding_institution_ids":["https://openalex.org/I87445476"],"apc_list":{"value":4011,"currency":"USD","value_usd":4011},"apc_paid":null,"fwci":0.4333,"has_fulltext":false,"cited_by_count":6,"citation_normalized_percentile":{"value":0.57762271,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":99},"biblio":{"volume":"23","issue":"5","first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.9997000098228455,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.9997000098228455,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10207","display_name":"Advanced biosensing and bioanalysis techniques","score":0.9987999796867371,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10725","display_name":"RNA Interference and Gene Delivery","score":0.9987000226974487,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/crispr","display_name":"CRISPR","score":0.7961720824241638},{"id":"https://openalex.org/keywords/guide-rna","display_name":"Guide RNA","score":0.7644567489624023},{"id":"https://openalex.org/keywords/cas9","display_name":"Cas9","score":0.7419001460075378},{"id":"https://openalex.org/keywords/computer-science","display_name":"Computer science","score":0.7030104994773865},{"id":"https://openalex.org/keywords/genome-editing","display_name":"Genome editing","score":0.6477429270744324},{"id":"https://openalex.org/keywords/subgenomic-mrna","display_name":"Subgenomic mRNA","score":0.6106881499290466},{"id":"https://openalex.org/keywords/computational-biology","display_name":"Computational biology","score":0.426509827375412},{"id":"https://openalex.org/keywords/biology","display_name":"Biology","score":0.24819791316986084},{"id":"https://openalex.org/keywords/genetics","display_name":"Genetics","score":0.19478479027748108},{"id":"https://openalex.org/keywords/gene","display_name":"Gene","score":0.17575886845588684}],"concepts":[{"id":"https://openalex.org/C98108389","wikidata":"https://www.wikidata.org/wiki/Q412563","display_name":"CRISPR","level":3,"score":0.7961720824241638},{"id":"https://openalex.org/C97702854","wikidata":"https://www.wikidata.org/wiki/Q4039747","display_name":"Guide RNA","level":5,"score":0.7644567489624023},{"id":"https://openalex.org/C132455925","wikidata":"https://www.wikidata.org/wiki/Q16965677","display_name":"Cas9","level":4,"score":0.7419001460075378},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.7030104994773865},{"id":"https://openalex.org/C144501496","wikidata":"https://www.wikidata.org/wiki/Q5533489","display_name":"Genome editing","level":4,"score":0.6477429270744324},{"id":"https://openalex.org/C189819185","wikidata":"https://www.wikidata.org/wiki/Q384283","display_name":"Subgenomic mRNA","level":4,"score":0.6106881499290466},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.426509827375412},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.24819791316986084},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.19478479027748108},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.17575886845588684}],"mesh":[{"descriptor_ui":"D000072669","descriptor_name":"Gene Editing","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D000072669","descriptor_name":"Gene Editing","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D000072669","descriptor_name":"Gene Editing","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008156","descriptor_name":"Luciferases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D008156","descriptor_name":"Luciferases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D008156","descriptor_name":"Luciferases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D008156","descriptor_name":"Luciferases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D008156","descriptor_name":"Luciferases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D008156","descriptor_name":"Luciferases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D058727","descriptor_name":"RNA, Small Untranslated","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D058727","descriptor_name":"RNA, Small Untranslated","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D058727","descriptor_name":"RNA, Small Untranslated","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D058727","descriptor_name":"RNA, Small Untranslated","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D058727","descriptor_name":"RNA, Small Untranslated","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D058727","descriptor_name":"RNA, Small Untranslated","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D064113","descriptor_name":"CRISPR-Cas Systems","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D064113","descriptor_name":"CRISPR-Cas Systems","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D064113","descriptor_name":"CRISPR-Cas Systems","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1093/bib/bbac183","is_oa":false,"landing_page_url":"https://doi.org/10.1093/bib/bbac183","pdf_url":null,"source":{"id":"https://openalex.org/S91767247","display_name":"Briefings in Bioinformatics","issn_l":"1467-5463","issn":["1467-5463","1477-4054"],"is_oa":false,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Briefings in Bioinformatics","raw_type":"journal-article"},{"id":"pmid:35580855","is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/35580855","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Briefings in bioinformatics","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[],"awards":[{"id":"https://openalex.org/G1206686860","display_name":null,"funder_award_id":"32170616","funder_id":"https://openalex.org/F4320321001","funder_display_name":"National Natural Science Foundation of China"},{"id":"https://openalex.org/G1709188862","display_name":null,"funder_award_id":"2020M683454","funder_id":"https://openalex.org/F4320321543","funder_display_name":"China Postdoctoral Science Foundation"},{"id":"https://openalex.org/G184507092","display_name":null,"funder_award_id":"31970569","funder_id":"https://openalex.org/F4320321001","funder_display_name":"National Natural Science Foundation of China"},{"id":"https://openalex.org/G55411952","display_name":null,"funder_award_id":"LWY20H060001","funder_id":"https://openalex.org/F4320338464","funder_display_name":"Natural Science Foundation of Zhejiang Province"},{"id":"https://openalex.org/G5720775178","display_name":null,"funder_award_id":"2021T140546","funder_id":"https://openalex.org/F4320321543","funder_display_name":"China Postdoctoral Science Foundation"},{"id":"https://openalex.org/G6764795550","display_name":null,"funder_award_id":"82170896","funder_id":"https://openalex.org/F4320321001","funder_display_name":"National Natural Science Foundation of China"},{"id":"https://openalex.org/G7697947464","display_name":null,"funder_award_id":"31871264","funder_id":"https://openalex.org/F4320321001","funder_display_name":"National Natural Science Foundation of China"},{"id":"https://openalex.org/G8019535394","display_name":null,"funder_award_id":"2021JC-02","funder_id":"https://openalex.org/F4320336567","funder_display_name":"Natural Science Basic Research Program of Shaanxi Province"}],"funders":[{"id":"https://openalex.org/F4320321001","display_name":"National Natural Science Foundation of China","ror":"https://ror.org/01h0zpd94"},{"id":"https://openalex.org/F4320321543","display_name":"China Postdoctoral Science Foundation","ror":"https://ror.org/0426zh255"},{"id":"https://openalex.org/F4320335787","display_name":"Fundamental Research Funds for the Central Universities","ror":null},{"id":"https://openalex.org/F4320336567","display_name":"Natural Science Basic Research Program of Shaanxi Province","ror":null},{"id":"https://openalex.org/F4320338464","display_name":"Natural Science Foundation of Zhejiang Province","ror":"https://ror.org/01h0zpd94"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":39,"referenced_works":["https://openalex.org/W1869792624","https://openalex.org/W1902237438","https://openalex.org/W1915704240","https://openalex.org/W1968342623","https://openalex.org/W2003171404","https://openalex.org/W2003797386","https://openalex.org/W2006373538","https://openalex.org/W2031211461","https://openalex.org/W2036176224","https://openalex.org/W2115603949","https://openalex.org/W2130942839","https://openalex.org/W2133564696","https://openalex.org/W2157331557","https://openalex.org/W2252568502","https://openalex.org/W2295107390","https://openalex.org/W2461013690","https://openalex.org/W2525778437","https://openalex.org/W2726053863","https://openalex.org/W2782551527","https://openalex.org/W2810756255","https://openalex.org/W2898598946","https://openalex.org/W2902305894","https://openalex.org/W2913423296","https://openalex.org/W2945567049","https://openalex.org/W2962851944","https://openalex.org/W2969911760","https://openalex.org/W2973312556","https://openalex.org/W3000149627","https://openalex.org/W3004131332","https://openalex.org/W3093163365","https://openalex.org/W3120026443","https://openalex.org/W3126072910","https://openalex.org/W3181661743","https://openalex.org/W4211192717","https://openalex.org/W6679434410","https://openalex.org/W6679436768","https://openalex.org/W6685133223","https://openalex.org/W6727690538","https://openalex.org/W6789990112"],"related_works":["https://openalex.org/W2794852126","https://openalex.org/W2529755192","https://openalex.org/W2134298288","https://openalex.org/W2951741674","https://openalex.org/W3105994629","https://openalex.org/W2994205747","https://openalex.org/W1869792624","https://openalex.org/W2968575375","https://openalex.org/W2565300371","https://openalex.org/W4213043670"],"abstract_inverted_index":{"Clustered":[0],"regularly":[1],"interspaced":[2],"short":[3],"palindromic":[4],"repeats":[5],"(CRISPR)/Cas9":[6],"gene":[7],"editing":[8],"technology":[9],"has":[10],"been":[11],"widely":[12],"used":[13],"to":[14,30,71,134],"facilitate":[15],"efficient":[16],"genome":[17],"editing.":[18],"Current":[19],"popular":[20],"sgRNA":[21,27,53,123],"design":[22,124],"tools":[23],"only":[24],"consider":[25],"the":[26,31,36,90,93,97,105,150],"perfectly":[28],"matched":[29,99],"target":[32,132],"site":[33],"and":[34,58,137,144],"provide":[35,51],"results":[37],"without":[38],"any":[39],"on-target":[40,45,76,113,146],"mismatch.":[41],"We":[42],"suppose":[43],"taking":[44],"gRNA-DNA":[46],"mismatches":[47,87],"into":[48],"consideration":[49],"might":[50],"better":[52],"with":[54,68,75,85,112,141,149],"similar":[55,145],"binding":[56],"activity":[57,95,147],"reduced":[59],"off-target":[60,108],"sites.":[61],"Here,":[62],"we":[63,116],"trained":[64],"a":[65,118],"seq2seq-attention":[66],"model":[67],"feedback-loop":[69],"architecture,":[70],"automatically":[72],"generate":[73],"sgRNAs":[74,84,111,140],"mismatches.":[77,114],"Dual-luciferase":[78],"reporter":[79],"experiment":[80],"showed":[81],"that":[82],"multiple":[83],"three":[86],"could":[88,103,129],"achieve":[89],"80%":[91],"of":[92,96,107],"relative":[94],"perfect":[98],"sgRNA.":[100,152],"Meanwhile,":[101],"it":[102],"reduce":[104],"number":[106],"sites":[109],"using":[110],"Finally,":[115],"provided":[117],"freely":[119],"accessible":[120],"web":[121],"server":[122,136],"tool":[125],"named":[126],"ExsgRNA.":[127],"Users":[128],"submit":[130],"their":[131],"sequence":[133],"this":[135],"get":[138],"optimal":[139],"less":[142],"off-targets":[143],"compared":[148],"perfect-matched":[151]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":2},{"year":2024,"cited_by_count":2},{"year":2023,"cited_by_count":1}],"updated_date":"2026-03-20T23:20:44.827607","created_date":"2025-10-10T00:00:00"}