{"id":"https://openalex.org/W3085797312","doi":"https://doi.org/10.1093/bib/bbaa193","title":"Identification of the RNase-binding site of SARS-CoV-2 RNA for anchor primer-PCR detection of viral loading in 306 COVID-19 patients","display_name":"Identification of the RNase-binding site of SARS-CoV-2 RNA for anchor primer-PCR detection of viral loading in 306 COVID-19 patients","publication_year":2020,"publication_date":"2020-08-11","ids":{"openalex":"https://openalex.org/W3085797312","doi":"https://doi.org/10.1093/bib/bbaa193","mag":"3085797312","pmid":"https://pubmed.ncbi.nlm.nih.gov/32935831"},"language":"en","primary_location":{"id":"doi:10.1093/bib/bbaa193","is_oa":true,"landing_page_url":"https://doi.org/10.1093/bib/bbaa193","pdf_url":"https://academic.oup.com/bib/article-pdf/22/2/1215/36654027/bbaa193.pdf","source":{"id":"https://openalex.org/S91767247","display_name":"Briefings in Bioinformatics","issn_l":"1467-5463","issn":["1467-5463","1477-4054"],"is_oa":false,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Briefings in Bioinformatics","raw_type":"journal-article"},"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://academic.oup.com/bib/article-pdf/22/2/1215/36654027/bbaa193.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5100621908","display_name":"Tao Xu","orcid":"https://orcid.org/0000-0003-2234-7737"},"institutions":[{"id":"https://openalex.org/I27781120","display_name":"Wenzhou Medical University","ror":"https://ror.org/00rd5t069","country_code":"CN","type":"education","lineage":["https://openalex.org/I27781120"]}],"countries":["CN"],"is_corresponding":true,"raw_author_name":"Tao Xu","raw_affiliation_strings":["Wenzhou Medical University"],"affiliations":[{"raw_affiliation_string":"Wenzhou Medical University","institution_ids":["https://openalex.org/I27781120"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5017812308","display_name":"Jingu Wang","orcid":null},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Jingu Wang","raw_affiliation_strings":[],"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5029937389","display_name":"Bingjie Hu","orcid":"https://orcid.org/0000-0002-6677-9454"},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Bingjie Hu","raw_affiliation_strings":["WMU International Eye Hospital of Taizhou"],"affiliations":[{"raw_affiliation_string":"WMU International Eye Hospital of Taizhou","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5004403062","display_name":"Guosi Zhang","orcid":null},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Guosi Zhang","raw_affiliation_strings":["School of Optometry and Ophthalmology of Wmu"],"affiliations":[{"raw_affiliation_string":"School of Optometry and Ophthalmology of Wmu","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101752811","display_name":"Wu Zhou","orcid":"https://orcid.org/0000-0002-0965-9915"},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Wu Zhou","raw_affiliation_strings":["First Affiliated Hospital of Wmu"],"affiliations":[{"raw_affiliation_string":"First Affiliated Hospital of Wmu","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5086737251","display_name":"Meiqin Zheng","orcid":"https://orcid.org/0000-0002-3249-6683"},"institutions":[{"id":"https://openalex.org/I27781120","display_name":"Wenzhou Medical University","ror":"https://ror.org/00rd5t069","country_code":"CN","type":"education","lineage":["https://openalex.org/I27781120"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Meiqin Zheng","raw_affiliation_strings":["Wenzhou Medical University"],"affiliations":[{"raw_affiliation_string":"Wenzhou Medical University","institution_ids":["https://openalex.org/I27781120"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5026505563","display_name":"Bo Shen","orcid":"https://orcid.org/0000-0002-1662-3558"},"institutions":[{"id":"https://openalex.org/I27781120","display_name":"Wenzhou Medical University","ror":"https://ror.org/00rd5t069","country_code":"CN","type":"education","lineage":["https://openalex.org/I27781120"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Bo Shen","raw_affiliation_strings":["Wenzhou Medical University"],"affiliations":[{"raw_affiliation_string":"Wenzhou Medical University","institution_ids":["https://openalex.org/I27781120"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5054443137","display_name":"Bao\u2010Chang Sun","orcid":"https://orcid.org/0000-0002-3435-1250"},"institutions":[{"id":"https://openalex.org/I4210140459","display_name":"Center for Disease Control","ror":"https://ror.org/04hsdam77","country_code":"TW","type":"facility","lineage":["https://openalex.org/I4210140459"]}],"countries":["TW"],"is_corresponding":false,"raw_author_name":"Baochang Sun","raw_affiliation_strings":["Wenzhou Center for Disease Control and Prevention"],"affiliations":[{"raw_affiliation_string":"Wenzhou Center for Disease Control and Prevention","institution_ids":["https://openalex.org/I4210140459"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100449646","display_name":"Yanjun Zhang","orcid":"https://orcid.org/0000-0002-9597-2050"},"institutions":[{"id":"https://openalex.org/I114539943","display_name":"Zhejiang Chinese Medical University","ror":"https://ror.org/04epb4p87","country_code":"CN","type":"education","lineage":["https://openalex.org/I114539943"]},{"id":"https://openalex.org/I4210136416","display_name":"Zhejiang Center for Disease Control and Prevention","ror":"https://ror.org/03f015z81","country_code":"CN","type":"government","lineage":["https://openalex.org/I4210136416"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Yanjun Zhang","raw_affiliation_strings":["Zhejiang Chinese Medical University; Zhejiang Provincial Center for Disease Control and Prevention"],"affiliations":[{"raw_affiliation_string":"Zhejiang Chinese Medical University; Zhejiang Provincial Center for Disease Control and Prevention","institution_ids":["https://openalex.org/I4210136416","https://openalex.org/I114539943"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100430556","display_name":"Yin Chen","orcid":"https://orcid.org/0000-0002-2191-6873"},"institutions":[{"id":"https://openalex.org/I4210136416","display_name":"Zhejiang Center for Disease Control and Prevention","ror":"https://ror.org/03f015z81","country_code":"CN","type":"government","lineage":["https://openalex.org/I4210136416"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Yin Chen","raw_affiliation_strings":["Zhejiang Provincial Center for Disease Control and Prevention"],"affiliations":[{"raw_affiliation_string":"Zhejiang Provincial Center for Disease Control and Prevention","institution_ids":["https://openalex.org/I4210136416"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5001206769","display_name":"Jian Yu","orcid":"https://orcid.org/0000-0001-8604-4538"},"institutions":[{"id":"https://openalex.org/I4210099512","display_name":"Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University","ror":"https://ror.org/0156rhd17","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210099512"]},{"id":"https://openalex.org/I27781120","display_name":"Wenzhou Medical University","ror":"https://ror.org/00rd5t069","country_code":"CN","type":"education","lineage":["https://openalex.org/I27781120"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Jian Yu","raw_affiliation_strings":["The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University"],"affiliations":[{"raw_affiliation_string":"The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University","institution_ids":["https://openalex.org/I4210099512","https://openalex.org/I27781120"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5060552645","display_name":"Min Liang","orcid":"https://orcid.org/0000-0002-0613-2077"},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Min Liang","raw_affiliation_strings":["First Affiliated Hospital of Wmu"],"affiliations":[{"raw_affiliation_string":"First Affiliated Hospital of Wmu","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5039059485","display_name":"Jingye Pan","orcid":"https://orcid.org/0000-0002-2367-1275"},"institutions":[{"id":"https://openalex.org/I27781120","display_name":"Wenzhou Medical University","ror":"https://ror.org/00rd5t069","country_code":"CN","type":"education","lineage":["https://openalex.org/I27781120"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Jingye Pan","raw_affiliation_strings":["Wenzhou Medical University"],"affiliations":[{"raw_affiliation_string":"Wenzhou Medical University","institution_ids":["https://openalex.org/I27781120"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101531591","display_name":"Chengshui Chen","orcid":"https://orcid.org/0000-0003-4841-9911"},"institutions":[{"id":"https://openalex.org/I27781120","display_name":"Wenzhou Medical University","ror":"https://ror.org/00rd5t069","country_code":"CN","type":"education","lineage":["https://openalex.org/I27781120"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Chengshui Chen","raw_affiliation_strings":["Wenzhou Medical University"],"affiliations":[{"raw_affiliation_string":"Wenzhou Medical University","institution_ids":["https://openalex.org/I27781120"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5083890521","display_name":"Haixiao Chen","orcid":"https://orcid.org/0000-0001-6556-1253"},"institutions":[{"id":"https://openalex.org/I27781120","display_name":"Wenzhou Medical University","ror":"https://ror.org/00rd5t069","country_code":"CN","type":"education","lineage":["https://openalex.org/I27781120"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Haixiao Chen","raw_affiliation_strings":["Wenzhou Medical University"],"affiliations":[{"raw_affiliation_string":"Wenzhou Medical University","institution_ids":["https://openalex.org/I27781120"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5038863732","display_name":"Minghua Jiang","orcid":"https://orcid.org/0000-0001-6421-8613"},"institutions":[{"id":"https://openalex.org/I27781120","display_name":"Wenzhou Medical University","ror":"https://ror.org/00rd5t069","country_code":"CN","type":"education","lineage":["https://openalex.org/I27781120"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Minghua Jiang","raw_affiliation_strings":["Wenzhou Medical University"],"affiliations":[{"raw_affiliation_string":"Wenzhou Medical University","institution_ids":["https://openalex.org/I27781120"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5034047362","display_name":"Liangde Xu","orcid":"https://orcid.org/0000-0003-0965-9955"},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Liangde Xu","raw_affiliation_strings":["School of Optometry and Ophthalmology of Wmu"],"affiliations":[{"raw_affiliation_string":"School of Optometry and Ophthalmology of Wmu","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5068842292","display_name":"Jia Qu","orcid":"https://orcid.org/0000-0001-5729-567X"},"institutions":[{"id":"https://openalex.org/I27781120","display_name":"Wenzhou Medical University","ror":"https://ror.org/00rd5t069","country_code":"CN","type":"education","lineage":["https://openalex.org/I27781120"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Jia Qu","raw_affiliation_strings":["Wenzhou Medical University"],"affiliations":[{"raw_affiliation_string":"Wenzhou Medical University","institution_ids":["https://openalex.org/I27781120"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5072159453","display_name":"Jiang\u2010Fan Chen","orcid":"https://orcid.org/0000-0002-0446-3956"},"institutions":[{"id":"https://openalex.org/I27781120","display_name":"Wenzhou Medical University","ror":"https://ror.org/00rd5t069","country_code":"CN","type":"education","lineage":["https://openalex.org/I27781120"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Jiang-Fan Chen","raw_affiliation_strings":["Wenzhou Medical University"],"affiliations":[{"raw_affiliation_string":"Wenzhou Medical University","institution_ids":["https://openalex.org/I27781120"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":19,"corresponding_author_ids":["https://openalex.org/A5100621908"],"corresponding_institution_ids":["https://openalex.org/I27781120"],"apc_list":{"value":4011,"currency":"USD","value_usd":4011},"apc_paid":null,"fwci":0.1265,"has_fulltext":true,"cited_by_count":10,"citation_normalized_percentile":{"value":0.58106422,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":97},"biblio":{"volume":"22","issue":"2","first_page":"1215","last_page":"1224"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10118","display_name":"SARS-CoV-2 and COVID-19 Research","score":0.9998999834060669,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10118","display_name":"SARS-CoV-2 and COVID-19 Research","score":0.9998999834060669,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11754","display_name":"SARS-CoV-2 detection and testing","score":0.9998000264167786,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10976","display_name":"Viral gastroenteritis research and epidemiology","score":0.9995999932289124,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/rna","display_name":"RNA","score":0.7108414173126221},{"id":"https://openalex.org/keywords/rnase-p","display_name":"RNase P","score":0.6713134050369263},{"id":"https://openalex.org/keywords/virology","display_name":"Virology","score":0.5882291197776794},{"id":"https://openalex.org/keywords/rna-extraction","display_name":"RNA extraction","score":0.5085188150405884},{"id":"https://openalex.org/keywords/coronavirus","display_name":"Coronavirus","score":0.49726417660713196},{"id":"https://openalex.org/keywords/biology","display_name":"Biology","score":0.48544102907180786},{"id":"https://openalex.org/keywords/primer","display_name":"Primer (cosmetics)","score":0.4708249866962433},{"id":"https://openalex.org/keywords/gene","display_name":"Gene","score":0.4509057104587555},{"id":"https://openalex.org/keywords/molecular-biology","display_name":"Molecular biology","score":0.4506138861179352},{"id":"https://openalex.org/keywords/rnase-h","display_name":"RNase H","score":0.4441899061203003},{"id":"https://openalex.org/keywords/coronavirus-disease-2019","display_name":"Coronavirus disease 2019 (COVID-19)","score":0.2565772533416748},{"id":"https://openalex.org/keywords/chemistry","display_name":"Chemistry","score":0.2453395128250122},{"id":"https://openalex.org/keywords/medicine","display_name":"Medicine","score":0.19493845105171204},{"id":"https://openalex.org/keywords/genetics","display_name":"Genetics","score":0.09299305081367493},{"id":"https://openalex.org/keywords/disease","display_name":"Disease","score":0.05040350556373596}],"concepts":[{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.7108414173126221},{"id":"https://openalex.org/C10879258","wikidata":"https://www.wikidata.org/wiki/Q76826611","display_name":"RNase P","level":4,"score":0.6713134050369263},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.5882291197776794},{"id":"https://openalex.org/C112190416","wikidata":"https://www.wikidata.org/wiki/Q2124450","display_name":"RNA extraction","level":4,"score":0.5085188150405884},{"id":"https://openalex.org/C2777648638","wikidata":"https://www.wikidata.org/wiki/Q57751738","display_name":"Coronavirus","level":5,"score":0.49726417660713196},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.48544102907180786},{"id":"https://openalex.org/C2777563447","wikidata":"https://www.wikidata.org/wiki/Q7243397","display_name":"Primer (cosmetics)","level":2,"score":0.4708249866962433},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.4509057104587555},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.4506138861179352},{"id":"https://openalex.org/C69766542","wikidata":"https://www.wikidata.org/wiki/Q3430179","display_name":"RNase H","level":5,"score":0.4441899061203003},{"id":"https://openalex.org/C3008058167","wikidata":"https://www.wikidata.org/wiki/Q84263196","display_name":"Coronavirus disease 2019 (COVID-19)","level":4,"score":0.2565772533416748},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.2453395128250122},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.19493845105171204},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.09299305081367493},{"id":"https://openalex.org/C2779134260","wikidata":"https://www.wikidata.org/wiki/Q12136","display_name":"Disease","level":2,"score":0.05040350556373596},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.0},{"id":"https://openalex.org/C524204448","wikidata":"https://www.wikidata.org/wiki/Q788926","display_name":"Infectious disease (medical specialty)","level":3,"score":0.0}],"mesh":[{"descriptor_ui":"D000086382","descriptor_name":"COVID-19","qualifier_ui":"Q000821","qualifier_name":"virology","is_major_topic":false},{"descriptor_ui":"D000086382","descriptor_name":"COVID-19","qualifier_ui":"Q000821","qualifier_name":"virology","is_major_topic":false},{"descriptor_ui":"D000086382","descriptor_name":"COVID-19","qualifier_ui":"Q000821","qualifier_name":"virology","is_major_topic":false},{"descriptor_ui":"D000086402","descriptor_name":"SARS-CoV-2","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D000086402","descriptor_name":"SARS-CoV-2","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D000086402","descriptor_name":"SARS-CoV-2","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D000086402","descriptor_name":"SARS-CoV-2","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D000086402","descriptor_name":"SARS-CoV-2","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D000086402","descriptor_name":"SARS-CoV-2","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D000086402","descriptor_name":"SARS-CoV-2","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D000086402","descriptor_name":"SARS-CoV-2","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D000086402","descriptor_name":"SARS-CoV-2","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D000368","descriptor_name":"Aged","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000368","descriptor_name":"Aged","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000368","descriptor_name":"Aged","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001665","descriptor_name":"Binding Sites","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001665","descriptor_name":"Binding Sites","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001665","descriptor_name":"Binding Sites","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005260","descriptor_name":"Female","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005260","descriptor_name":"Female","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005260","descriptor_name":"Female","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008297","descriptor_name":"Male","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008297","descriptor_name":"Male","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008297","descriptor_name":"Male","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012260","descriptor_name":"Ribonucleases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D012260","descriptor_name":"Ribonucleases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D012260","descriptor_name":"Ribonucleases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D012367","descriptor_name":"RNA, Viral","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D012367","descriptor_name":"RNA, Viral","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D012367","descriptor_name":"RNA, Viral","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D016133","descriptor_name":"Polymerase Chain Reaction","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D016133","descriptor_name":"Polymerase Chain Reaction","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D016133","descriptor_name":"Polymerase Chain Reaction","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D019562","descriptor_name":"Viral Load","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019562","descriptor_name":"Viral Load","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019562","descriptor_name":"Viral Load","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1093/bib/bbaa193","is_oa":true,"landing_page_url":"https://doi.org/10.1093/bib/bbaa193","pdf_url":"https://academic.oup.com/bib/article-pdf/22/2/1215/36654027/bbaa193.pdf","source":{"id":"https://openalex.org/S91767247","display_name":"Briefings in Bioinformatics","issn_l":"1467-5463","issn":["1467-5463","1477-4054"],"is_oa":false,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Briefings in Bioinformatics","raw_type":"journal-article"},{"id":"pmid:32935831","is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/32935831","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Briefings in bioinformatics","raw_type":null},{"id":"pmh:oai:pubmedcentral.nih.gov:7543284","is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/7543284","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Brief Bioinform","raw_type":"Text"}],"best_oa_location":{"id":"doi:10.1093/bib/bbaa193","is_oa":true,"landing_page_url":"https://doi.org/10.1093/bib/bbaa193","pdf_url":"https://academic.oup.com/bib/article-pdf/22/2/1215/36654027/bbaa193.pdf","source":{"id":"https://openalex.org/S91767247","display_name":"Briefings in Bioinformatics","issn_l":"1467-5463","issn":["1467-5463","1477-4054"],"is_oa":false,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Briefings in Bioinformatics","raw_type":"journal-article"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.8799999952316284,"display_name":"Good health and well-being"}],"awards":[],"funders":[{"id":"https://openalex.org/F4320310111","display_name":"Wenzhou Medical University","ror":"https://ror.org/00rd5t069"},{"id":"https://openalex.org/F4320332162","display_name":"Centers for Disease Control and Prevention","ror":"https://ror.org/042twtr12"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W3085797312.pdf","grobid_xml":"https://content.openalex.org/works/W3085797312.grobid-xml"},"referenced_works_count":55,"referenced_works":["https://openalex.org/W1894777043","https://openalex.org/W2025150647","https://openalex.org/W2052204012","https://openalex.org/W2064515831","https://openalex.org/W2076793019","https://openalex.org/W2129542667","https://openalex.org/W2522585255","https://openalex.org/W2525468044","https://openalex.org/W2568883211","https://openalex.org/W2579012589","https://openalex.org/W2777914188","https://openalex.org/W2806964547","https://openalex.org/W2914256318","https://openalex.org/W2996345365","https://openalex.org/W3001195213","https://openalex.org/W3001897055","https://openalex.org/W3002108456","https://openalex.org/W3004318991","https://openalex.org/W3005510968","https://openalex.org/W3006856666","https://openalex.org/W3007104924","https://openalex.org/W3007497549","https://openalex.org/W3008568763","https://openalex.org/W3008827533","https://openalex.org/W3009219040","https://openalex.org/W3009318907","https://openalex.org/W3009409276","https://openalex.org/W3010278110","https://openalex.org/W3010604545","https://openalex.org/W3011127849","https://openalex.org/W3012537262","https://openalex.org/W3012756997","https://openalex.org/W3013640245","https://openalex.org/W3013776441","https://openalex.org/W3013893137","https://openalex.org/W3014337446","https://openalex.org/W3014833803","https://openalex.org/W3014989172","https://openalex.org/W3015040045","https://openalex.org/W3015444032","https://openalex.org/W3015599013","https://openalex.org/W3015777742","https://openalex.org/W3016173697","https://openalex.org/W3016214835","https://openalex.org/W3017150431","https://openalex.org/W3025933799","https://openalex.org/W3026418408","https://openalex.org/W3027866910","https://openalex.org/W3033338463","https://openalex.org/W3045391095","https://openalex.org/W3157025007","https://openalex.org/W4242658674","https://openalex.org/W6759100428","https://openalex.org/W6774707739","https://openalex.org/W6775013915"],"related_works":["https://openalex.org/W2080304317","https://openalex.org/W1626305077","https://openalex.org/W2801209660","https://openalex.org/W1963782006","https://openalex.org/W2015009754","https://openalex.org/W1750017366","https://openalex.org/W4248828958","https://openalex.org/W2162241913","https://openalex.org/W2338873733","https://openalex.org/W1972874234"],"abstract_inverted_index":{"The":[0,67],"pandemic":[1],"of":[2,17,39,42,59,75,87,93,102,122,127,133,149,154,164,166,181,188,197],"coronavirus":[3,47],"disease":[4],"2019":[5],"(COVID-19)":[6],"urgently":[7],"calls":[8],"for":[9,200],"more":[10],"sensitive":[11],"molecular":[12,218],"diagnosis":[13],"to":[14,31,96,115],"improve":[15,32],"sensitivity":[16,142],"current":[18],"viral":[19,33,76,124,198,229],"nuclear":[20],"acid":[21],"detection.":[22],"We":[23],"have":[24],"developed":[25],"an":[26],"anchor":[27],"primer":[28],"(AP)-based":[29],"assay":[30],"RNA":[34,50,61,77,95,104,125,151,214,223],"stability":[35,126],"by":[36,81,105,143,147,220],"bioinformatics":[37],"identification":[38],"RNase-binding":[40],"site":[41],"severe":[43],"acute":[44],"respiratory":[45],"syndrome":[46],"2":[48],"(SARS-CoV-2)":[49],"and":[51,62,137,159,161,179,191,215,225,235],"implementing":[52],"AP":[53,84,210],"dually":[54,211],"targeting":[55,132,212],"the":[56,83,88,100,123,128,134,140,167,204,209,227],"N":[57,135],"gene":[58,90,130,136],"SARS-CoV-2":[60,94,103,150,213,222],"RNase":[63,97,216],"1,":[64],"3,":[65],"6.":[66],"arbitrarily":[68],"primed":[69],"polymerase":[70],"chain":[71],"reaction":[72],"(AP-PCR)":[73],"improvement":[74,121],"integrity":[78,224],"was":[79,145],"supported":[80],"(a)":[82],"increased":[85],"resistance":[86],"targeted":[89],"(N":[91],"gene)":[92],"treatment;":[98],"(b)":[99],"detection":[101,148,219],"AP-PCR":[106,144,186],"with":[107,232],"lower":[108],"cycle":[109],"threshold":[110],"values":[111],"(-2.7":[112],"cycles)":[113],"compared":[114],"two":[116],"commercially":[117],"available":[118],"assays;":[119],"(c)":[120],"ORF":[129],"upon":[131],"RNase.":[138],"Furthermore,":[139],"improved":[141],"demonstrated":[146],"in":[152,238],"70-80%":[153],"sputum,":[155],"nasal,":[156],"pharyngeal":[157],"swabs":[158],"feces":[160],"36%":[162],"(4/11)":[163],"urine":[165],"confirmed":[168,190],"cases":[169,175,193],"(n":[170,176],"=":[171,177],"252),":[172],"7%":[173],"convalescent":[174,192],"54)":[178],"none":[180],"300":[182],"negative":[183],"cases.":[184],"Lastly,":[185],"analysis":[187],"306":[189],"revealed":[194],"prolonged":[195,228],"presence":[196],"loading":[199,230],">20":[201],"days":[202],"after":[203],"first":[205],"positive":[206],"diagnosis.":[207],"Thus,":[208],"improves":[217],"preserving":[221],"reveals":[226],"associated":[231],"older":[233],"age":[234],"male":[236],"gender":[237],"COVID-19":[239],"patients.":[240]},"counts_by_year":[{"year":2025,"cited_by_count":2},{"year":2024,"cited_by_count":3},{"year":2023,"cited_by_count":2},{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":2}],"updated_date":"2025-11-06T03:46:38.306776","created_date":"2025-10-10T00:00:00"}