{"id":"https://openalex.org/W4402929442","doi":"https://doi.org/10.1021/acs.jcim.4c01389","title":"Mechanism of Dual-Site Recognition in a Classic DNA Aptamer","display_name":"Mechanism of Dual-Site Recognition in a Classic DNA Aptamer","publication_year":2024,"publication_date":"2024-09-27","ids":{"openalex":"https://openalex.org/W4402929442","doi":"https://doi.org/10.1021/acs.jcim.4c01389","pmid":"https://pubmed.ncbi.nlm.nih.gov/39327929"},"language":"en","primary_location":{"id":"doi:10.1021/acs.jcim.4c01389","is_oa":true,"landing_page_url":"https://doi.org/10.1021/acs.jcim.4c01389","pdf_url":null,"source":{"id":"https://openalex.org/S167262187","display_name":"Journal of Chemical Information and Modeling","issn_l":"1549-9596","issn":["1549-9596","1549-960X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Chemical Information and Modeling","raw_type":"journal-article"},"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"https://doi.org/10.1021/acs.jcim.4c01389","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5100350329","display_name":"Yunpeng Wang","orcid":"https://orcid.org/0000-0003-4280-7243"},"institutions":[{"id":"https://openalex.org/I125839683","display_name":"Beijing Institute of Technology","ror":"https://ror.org/01skt4w74","country_code":"CN","type":"education","lineage":["https://openalex.org/I125839683","https://openalex.org/I890469752"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Yun-Peng Wang","raw_affiliation_strings":["School of Chemistry and Chemical Engineering, Beijing Institute of Technology, South Street No. 5, Zhongguancun, Haidian District, Beijing 100081, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"School of Chemistry and Chemical Engineering, Beijing Institute of Technology, South Street No. 5, Zhongguancun, Haidian District, Beijing 100081, China","institution_ids":["https://openalex.org/I125839683"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5001315059","display_name":"Leif A. Eriksson","orcid":"https://orcid.org/0000-0001-5654-3109"},"institutions":[{"id":"https://openalex.org/I881427289","display_name":"University of Gothenburg","ror":"https://ror.org/01tm6cn81","country_code":"SE","type":"education","lineage":["https://openalex.org/I881427289"]}],"countries":["SE"],"is_corresponding":true,"raw_author_name":"Leif A. Eriksson","raw_affiliation_strings":["Department of Chemistry and Molecular Biology, University of Gothenburg, Medicinaregatan 7b, G\u00f6teborg 405 30, Sweden"],"raw_orcid":"https://orcid.org/0000-0001-5654-3109","affiliations":[{"raw_affiliation_string":"Department of Chemistry and Molecular Biology, University of Gothenburg, Medicinaregatan 7b, G\u00f6teborg 405 30, Sweden","institution_ids":["https://openalex.org/I881427289"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5101735546","display_name":"Rubo Zhang","orcid":"https://orcid.org/0000-0003-4822-0590"},"institutions":[{"id":"https://openalex.org/I125839683","display_name":"Beijing Institute of Technology","ror":"https://ror.org/01skt4w74","country_code":"CN","type":"education","lineage":["https://openalex.org/I125839683","https://openalex.org/I890469752"]}],"countries":["CN"],"is_corresponding":true,"raw_author_name":"Ru-Bo Zhang","raw_affiliation_strings":["School of Chemistry and Chemical Engineering, Beijing Institute of Technology, South Street No. 5, Zhongguancun, Haidian District, Beijing 100081, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"School of Chemistry and Chemical Engineering, Beijing Institute of Technology, South Street No. 5, Zhongguancun, Haidian District, Beijing 100081, China","institution_ids":["https://openalex.org/I125839683"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5001315059","https://openalex.org/A5101735546"],"corresponding_institution_ids":["https://openalex.org/I125839683","https://openalex.org/I881427289"],"apc_list":null,"apc_paid":null,"fwci":0.6421,"has_fulltext":true,"cited_by_count":3,"citation_normalized_percentile":{"value":0.69039156,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":95,"max":98},"biblio":{"volume":"64","issue":"19","first_page":"7698","last_page":"7708"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10207","display_name":"Advanced biosensing and bioanalysis techniques","score":0.9998999834060669,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10207","display_name":"Advanced biosensing and bioanalysis techniques","score":0.9998999834060669,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10432","display_name":"DNA and Nucleic Acid Chemistry","score":0.9998999834060669,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":0.9988999962806702,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/aptamer","display_name":"Aptamer","score":0.8250027298927307},{"id":"https://openalex.org/keywords/dna","display_name":"DNA","score":0.5830895304679871},{"id":"https://openalex.org/keywords/dual","display_name":"Dual (grammatical number)","score":0.5807614326477051},{"id":"https://openalex.org/keywords/computational-biology","display_name":"Computational biology","score":0.579295814037323},{"id":"https://openalex.org/keywords/mechanism","display_name":"Mechanism (biology)","score":0.5099970102310181},{"id":"https://openalex.org/keywords/chemistry","display_name":"Chemistry","score":0.396545946598053},{"id":"https://openalex.org/keywords/biology","display_name":"Biology","score":0.32920509576797485},{"id":"https://openalex.org/keywords/genetics","display_name":"Genetics","score":0.281274676322937},{"id":"https://openalex.org/keywords/physics","display_name":"Physics","score":0.0881386399269104}],"concepts":[{"id":"https://openalex.org/C128972844","wikidata":"https://www.wikidata.org/wiki/Q418722","display_name":"Aptamer","level":2,"score":0.8250027298927307},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.5830895304679871},{"id":"https://openalex.org/C2780980858","wikidata":"https://www.wikidata.org/wiki/Q110022","display_name":"Dual (grammatical number)","level":2,"score":0.5807614326477051},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.579295814037323},{"id":"https://openalex.org/C89611455","wikidata":"https://www.wikidata.org/wiki/Q6804646","display_name":"Mechanism (biology)","level":2,"score":0.5099970102310181},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.396545946598053},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.32920509576797485},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.281274676322937},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.0881386399269104},{"id":"https://openalex.org/C62520636","wikidata":"https://www.wikidata.org/wiki/Q944","display_name":"Quantum mechanics","level":1,"score":0.0},{"id":"https://openalex.org/C142362112","wikidata":"https://www.wikidata.org/wiki/Q735","display_name":"Art","level":0,"score":0.0},{"id":"https://openalex.org/C124952713","wikidata":"https://www.wikidata.org/wiki/Q8242","display_name":"Literature","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000249","descriptor_name":"Adenosine Monophosphate","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D000249","descriptor_name":"Adenosine Monophosphate","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D000249","descriptor_name":"Adenosine Monophosphate","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D000249","descriptor_name":"Adenosine Monophosphate","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D001665","descriptor_name":"Binding Sites","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001665","descriptor_name":"Binding Sites","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001665","descriptor_name":"Binding Sites","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001665","descriptor_name":"Binding Sites","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006860","descriptor_name":"Hydrogen Bonding","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006860","descriptor_name":"Hydrogen Bonding","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006860","descriptor_name":"Hydrogen Bonding","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006860","descriptor_name":"Hydrogen Bonding","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009690","descriptor_name":"Nucleic Acid Conformation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009690","descriptor_name":"Nucleic Acid Conformation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009690","descriptor_name":"Nucleic Acid Conformation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009690","descriptor_name":"Nucleic Acid Conformation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013816","descriptor_name":"Thermodynamics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013816","descriptor_name":"Thermodynamics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013816","descriptor_name":"Thermodynamics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013816","descriptor_name":"Thermodynamics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D052157","descriptor_name":"Aptamers, Nucleotide","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D052157","descriptor_name":"Aptamers, Nucleotide","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D052157","descriptor_name":"Aptamers, Nucleotide","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D052157","descriptor_name":"Aptamers, Nucleotide","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D052157","descriptor_name":"Aptamers, Nucleotide","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D052157","descriptor_name":"Aptamers, Nucleotide","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D052157","descriptor_name":"Aptamers, Nucleotide","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D052157","descriptor_name":"Aptamers, Nucleotide","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D056004","descriptor_name":"Molecular Dynamics Simulation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D056004","descriptor_name":"Molecular Dynamics Simulation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D056004","descriptor_name":"Molecular Dynamics Simulation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D056004","descriptor_name":"Molecular Dynamics Simulation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":3,"locations":[{"id":"doi:10.1021/acs.jcim.4c01389","is_oa":true,"landing_page_url":"https://doi.org/10.1021/acs.jcim.4c01389","pdf_url":null,"source":{"id":"https://openalex.org/S167262187","display_name":"Journal of Chemical Information and Modeling","issn_l":"1549-9596","issn":["1549-9596","1549-960X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Chemical Information and Modeling","raw_type":"journal-article"},{"id":"pmid:39327929","is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/39327929","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of chemical information and modeling","raw_type":null},{"id":"pmh:oai:pubmedcentral.nih.gov:11481096","is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/11481096","pdf_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC11481096/pdf/ci4c01389.pdf","source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"J Chem Inf Model","raw_type":"Text"}],"best_oa_location":{"id":"doi:10.1021/acs.jcim.4c01389","is_oa":true,"landing_page_url":"https://doi.org/10.1021/acs.jcim.4c01389","pdf_url":null,"source":{"id":"https://openalex.org/S167262187","display_name":"Journal of Chemical Information and Modeling","issn_l":"1549-9596","issn":["1549-9596","1549-960X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Chemical Information and Modeling","raw_type":"journal-article"},"sustainable_development_goals":[{"score":0.5600000023841858,"id":"https://metadata.un.org/sdg/2","display_name":"Zero hunger"}],"awards":[{"id":"https://openalex.org/G4023234063","display_name":null,"funder_award_id":"211447-Pj","funder_id":"https://openalex.org/F4320321491","funder_display_name":"Cancerfonden"},{"id":"https://openalex.org/G6150852475","display_name":null,"funder_award_id":"22177013","funder_id":"https://openalex.org/F4320321001","funder_display_name":"National Natural Science Foundation of China"},{"id":"https://openalex.org/G6854911798","display_name":null,"funder_award_id":"211447-Pj","funder_id":"https://openalex.org/F4320313607","funder_display_name":"Swedish Cancer Foundation"}],"funders":[{"id":"https://openalex.org/F4320313607","display_name":"Swedish Cancer Foundation","ror":null},{"id":"https://openalex.org/F4320321001","display_name":"National Natural Science Foundation of China","ror":"https://ror.org/01h0zpd94"},{"id":"https://openalex.org/F4320321491","display_name":"Cancerfonden","ror":"https://ror.org/0527jb766"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":52,"referenced_works":["https://openalex.org/W1966078827","https://openalex.org/W1970478438","https://openalex.org/W1972625271","https://openalex.org/W1981021420","https://openalex.org/W2026520402","https://openalex.org/W2029667189","https://openalex.org/W2035266068","https://openalex.org/W2044591029","https://openalex.org/W2045161306","https://openalex.org/W2057477511","https://openalex.org/W2058971275","https://openalex.org/W2065601399","https://openalex.org/W2066661864","https://openalex.org/W2067174909","https://openalex.org/W2079321730","https://openalex.org/W2079632539","https://openalex.org/W2096747776","https://openalex.org/W2128572087","https://openalex.org/W2131841865","https://openalex.org/W2132525235","https://openalex.org/W2147993766","https://openalex.org/W2154379876","https://openalex.org/W2157052506","https://openalex.org/W2171268876","https://openalex.org/W2206954826","https://openalex.org/W2295087691","https://openalex.org/W2322090998","https://openalex.org/W2327513492","https://openalex.org/W2334865985","https://openalex.org/W2433523887","https://openalex.org/W2463208053","https://openalex.org/W2605443933","https://openalex.org/W2623017876","https://openalex.org/W2761777466","https://openalex.org/W2885128148","https://openalex.org/W2922026077","https://openalex.org/W2982862739","https://openalex.org/W3004940160","https://openalex.org/W3025988887","https://openalex.org/W3027187308","https://openalex.org/W3042908073","https://openalex.org/W3086801715","https://openalex.org/W3095438497","https://openalex.org/W3133678705","https://openalex.org/W3171458855","https://openalex.org/W3188164666","https://openalex.org/W3196119574","https://openalex.org/W3205686806","https://openalex.org/W4206131914","https://openalex.org/W4292084531","https://openalex.org/W4293067229","https://openalex.org/W4313454382"],"related_works":["https://openalex.org/W2967136651","https://openalex.org/W2913646003","https://openalex.org/W2033684641","https://openalex.org/W2081632393","https://openalex.org/W2147509954","https://openalex.org/W4389944751","https://openalex.org/W1970773045","https://openalex.org/W3092671456","https://openalex.org/W2566736735","https://openalex.org/W2898457449"],"abstract_inverted_index":{"Nucleic":[0],"acid":[1],"aptamers":[2],"possess":[3],"unique":[4],"advantages":[5],"in":[6,28,55,72,134,154,191],"specific":[7],"recognition.":[8],"However,":[9],"the":[10,51,56,61,75,85,98,102,106,113,135,141,155,159,162,166,177,184],"lack":[11],"of":[12,46,81,84,94,108,128,180],"in-depth":[13],"investigation":[14],"into":[15],"their":[16,22],"dynamic":[17,62],"recognition":[18,63,164],"mechanisms":[19],"has":[20],"restricted":[21],"rational":[23],"design":[24],"and":[25,33,65,170,188],"potential":[26],"applications":[27],"fields":[29],"such":[30],"as":[31],"biosensing":[32],"targeted":[34],"therapy.":[35],"We":[36],"herein":[37],"utilized":[38],"enhanced":[39],"sampling":[40],"molecular":[41],"dynamics":[42],"to":[43,50,74,90,97,101,130,148,158],"address":[44],"affinities":[45],"adenosine":[47],"monophosphate":[48],"(AMP)":[49],"dual":[52],"binding":[53,87,96,117,137,142,194],"sites":[54,88],"DNA":[57],"aptamer,":[58],"focusing":[59],"on":[60],"mechanism":[64],"pathways.":[66],"The":[67],"present":[68],"results":[69],"indicate":[70],"that":[71],"addition":[73],"widely":[76],"known":[77],"intermolecular":[78],"interactions,":[79],"inequivalence":[80],"chemical":[82],"environments":[83],"two":[86,109,114],"leads":[89],"slightly":[91],"higher":[92],"stability":[93],"AMP":[95,129,185],"site":[99,156],"proximal":[100],"aptamer":[103],"terminus.":[104],"In":[105,122],"presence":[107],"AMPs":[110],"captured":[111],"by":[112],"sites,":[115],"each":[116],"free":[118,167,172],"energy":[119,143,168,173],"is":[120,132],"enhanced.":[121],"particular,":[123],"an":[124],"additional":[125],"hydrogen":[126],"bond":[127],"A10":[131],"introduced":[133],"dual-site":[136,163],"complex,":[138],"which":[139],"increases":[140],"from":[144],"\u22124.25":[145],"\u00b1":[146,150],"0.47":[147],"\u22129.48":[149],"0.33":[151],"kcal":[152],"mol\u20131":[153],"close":[157],"loop.":[160],"For":[161],"process,":[165],"landscape":[169],"minimum":[171],"pathway":[174],"calculations":[175],"elucidate":[176],"crucial":[178],"role":[179],"electrostatic":[181],"interactions":[182],"between":[183],"phosphate":[186],"groups":[187],"Na+":[189],"ions":[190],"positively":[192],"cooperative":[193],"mechanisms.":[195]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":2}],"updated_date":"2026-05-16T08:24:45.110214","created_date":"2025-10-10T00:00:00"}