{"id":"https://openalex.org/W2941874147","doi":"https://doi.org/10.1007/s40484-019-0166-9","title":"Current challenges and solutions of <i><b>de novo</b></i> assembly","display_name":"Current challenges and solutions of <i><b>de novo</b></i> assembly","publication_year":2019,"publication_date":"2019-04-25","ids":{"openalex":"https://openalex.org/W2941874147","doi":"https://doi.org/10.1007/s40484-019-0166-9","mag":"2941874147"},"language":"en","primary_location":{"id":"doi:10.1007/s40484-019-0166-9","is_oa":true,"landing_page_url":"https://doi.org/10.1007/s40484-019-0166-9","pdf_url":"https://link.springer.com/content/pdf/10.1007/s40484-019-0166-9.pdf","source":{"id":"https://openalex.org/S4210172333","display_name":"Quantitative Biology","issn_l":"2095-4689","issn":["2095-4689","2095-4697"],"is_oa":true,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Quantitative Biology","raw_type":"journal-article"},"type":"article","indexed_in":["crossref","doaj"],"open_access":{"is_oa":true,"oa_status":"diamond","oa_url":"https://link.springer.com/content/pdf/10.1007/s40484-019-0166-9.pdf","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5088826157","display_name":"Xingyu Liao","orcid":"https://orcid.org/0000-0002-0061-1317"},"institutions":[{"id":"https://openalex.org/I139660479","display_name":"Central South University","ror":"https://ror.org/00f1zfq44","country_code":"CN","type":"education","lineage":["https://openalex.org/I139660479"]}],"countries":["CN"],"is_corresponding":true,"raw_author_name":"Xingyu Liao","raw_affiliation_strings":["<!--1--> School of Computer Science and Engineering Central south University Changsha 410083 China","1. School of Computer Science and Engineering, Central south University, Changsha 410083, China"],"affiliations":[{"raw_affiliation_string":"<!--1--> School of Computer Science and Engineering Central south University Changsha 410083 China","institution_ids":["https://openalex.org/I139660479"]},{"raw_affiliation_string":"1. School of Computer Science and Engineering, Central south University, Changsha 410083, China","institution_ids":["https://openalex.org/I139660479"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100719013","display_name":"Min Li","orcid":"https://orcid.org/0000-0002-0188-1394"},"institutions":[{"id":"https://openalex.org/I139660479","display_name":"Central South University","ror":"https://ror.org/00f1zfq44","country_code":"CN","type":"education","lineage":["https://openalex.org/I139660479"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Min Li","raw_affiliation_strings":["<!--1--> School of Computer Science and Engineering Central south University Changsha 410083 China","1. School of Computer Science and Engineering, Central south University, Changsha 410083, China"],"affiliations":[{"raw_affiliation_string":"<!--1--> School of Computer Science and Engineering Central south University Changsha 410083 China","institution_ids":["https://openalex.org/I139660479"]},{"raw_affiliation_string":"1. School of Computer Science and Engineering, Central south University, Changsha 410083, China","institution_ids":["https://openalex.org/I139660479"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5022432765","display_name":"You Zou","orcid":null},"institutions":[{"id":"https://openalex.org/I139660479","display_name":"Central South University","ror":"https://ror.org/00f1zfq44","country_code":"CN","type":"education","lineage":["https://openalex.org/I139660479"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"You Zou","raw_affiliation_strings":["<!--1--> School of Computer Science and Engineering Central south University Changsha 410083 China","1. School of Computer Science and Engineering, Central south University, Changsha 410083, China"],"affiliations":[{"raw_affiliation_string":"<!--1--> School of Computer Science and Engineering Central south University Changsha 410083 China","institution_ids":["https://openalex.org/I139660479"]},{"raw_affiliation_string":"1. School of Computer Science and Engineering, Central south University, Changsha 410083, China","institution_ids":["https://openalex.org/I139660479"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5091157395","display_name":"Fang\u2010Xiang Wu","orcid":"https://orcid.org/0000-0002-4593-9332"},"institutions":[{"id":"https://openalex.org/I32625721","display_name":"University of Saskatchewan","ror":"https://ror.org/010x8gc63","country_code":"CA","type":"education","lineage":["https://openalex.org/I32625721"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Fang\u2010Xiang Wu","raw_affiliation_strings":["<!--2--> Division of Biomedical Engineering University of Saskatchewan Saskatchewan S7N 5A9 Canada","2. Division of Biomedical Engineering, University of Saskatchewan, Saskatchewan, S7N 5A9, Canada"],"affiliations":[{"raw_affiliation_string":"<!--2--> Division of Biomedical Engineering University of Saskatchewan Saskatchewan S7N 5A9 Canada","institution_ids":["https://openalex.org/I32625721"]},{"raw_affiliation_string":"2. Division of Biomedical Engineering, University of Saskatchewan, Saskatchewan, S7N 5A9, Canada","institution_ids":["https://openalex.org/I32625721"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5108680423","display_name":"Yi\u2010Pan","orcid":null},"institutions":[{"id":"https://openalex.org/I181565077","display_name":"Georgia State University","ror":"https://ror.org/03qt6ba18","country_code":"US","type":"education","lineage":["https://openalex.org/I181565077"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Yi\u2010Pan","raw_affiliation_strings":["<!--3--> Department of Computer Science Georgia State University Atlanta GA 30302 USA","3. Department of Computer Science, Georgia State University, Atlanta, GA 30302, USA"],"affiliations":[{"raw_affiliation_string":"<!--3--> Department of Computer Science Georgia State University Atlanta GA 30302 USA","institution_ids":["https://openalex.org/I181565077"]},{"raw_affiliation_string":"3. Department of Computer Science, Georgia State University, Atlanta, GA 30302, USA","institution_ids":["https://openalex.org/I181565077"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5100438360","display_name":"Jianxin Wang","orcid":"https://orcid.org/0000-0003-1516-0480"},"institutions":[{"id":"https://openalex.org/I139660479","display_name":"Central South University","ror":"https://ror.org/00f1zfq44","country_code":"CN","type":"education","lineage":["https://openalex.org/I139660479"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Jianxin Wang","raw_affiliation_strings":["<!--1--> School of Computer Science and Engineering Central south University Changsha 410083 China","1. School of Computer Science and Engineering, Central south University, Changsha 410083, China"],"affiliations":[{"raw_affiliation_string":"<!--1--> School of Computer Science and Engineering Central south University Changsha 410083 China","institution_ids":["https://openalex.org/I139660479"]},{"raw_affiliation_string":"1. School of Computer Science and Engineering, Central south University, Changsha 410083, China","institution_ids":["https://openalex.org/I139660479"]}]}],"institutions":[],"countries_distinct_count":3,"institutions_distinct_count":6,"corresponding_author_ids":["https://openalex.org/A5088826157"],"corresponding_institution_ids":["https://openalex.org/I139660479"],"apc_list":null,"apc_paid":null,"fwci":2.4925,"has_fulltext":true,"cited_by_count":90,"citation_normalized_percentile":{"value":0.90018711,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":97,"max":100},"biblio":{"volume":"7","issue":"2","first_page":"90","last_page":"109"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10015","display_name":"Genomics and Phylogenetic Studies","score":0.9986000061035156,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10015","display_name":"Genomics and Phylogenetic Studies","score":0.9986000061035156,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10207","display_name":"Advanced biosensing and bioanalysis techniques","score":0.9891999959945679,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11879","display_name":"Protist diversity and phylogeny","score":0.9717000126838684,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/de-bruijn-graph","display_name":"De Bruijn graph","score":0.7834866046905518},{"id":"https://openalex.org/keywords/sequence-assembly","display_name":"Sequence assembly","score":0.7600587606430054},{"id":"https://openalex.org/keywords/computer-science","display_name":"Computer science","score":0.6206815838813782},{"id":"https://openalex.org/keywords/hybrid-genome-assembly","display_name":"Hybrid genome assembly","score":0.4804919958114624},{"id":"https://openalex.org/keywords/dna-sequencing","display_name":"DNA sequencing","score":0.45633262395858765},{"id":"https://openalex.org/keywords/graph","display_name":"Graph","score":0.4545237123966217},{"id":"https://openalex.org/keywords/computational-biology","display_name":"Computational biology","score":0.3469703197479248},{"id":"https://openalex.org/keywords/biology","display_name":"Biology","score":0.2337789535522461},{"id":"https://openalex.org/keywords/theoretical-computer-science","display_name":"Theoretical computer science","score":0.22006309032440186},{"id":"https://openalex.org/keywords/reference-genome","display_name":"Reference genome","score":0.21797719597816467},{"id":"https://openalex.org/keywords/gene","display_name":"Gene","score":0.10349652171134949}],"concepts":[{"id":"https://openalex.org/C20218877","wikidata":"https://www.wikidata.org/wiki/Q3066095","display_name":"De Bruijn graph","level":3,"score":0.7834866046905518},{"id":"https://openalex.org/C18949551","wikidata":"https://www.wikidata.org/wiki/Q740578","display_name":"Sequence assembly","level":5,"score":0.7600587606430054},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.6206815838813782},{"id":"https://openalex.org/C113425843","wikidata":"https://www.wikidata.org/wiki/Q5953213","display_name":"Hybrid genome assembly","level":5,"score":0.4804919958114624},{"id":"https://openalex.org/C51679486","wikidata":"https://www.wikidata.org/wiki/Q380546","display_name":"DNA sequencing","level":3,"score":0.45633262395858765},{"id":"https://openalex.org/C132525143","wikidata":"https://www.wikidata.org/wiki/Q141488","display_name":"Graph","level":2,"score":0.4545237123966217},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.3469703197479248},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.2337789535522461},{"id":"https://openalex.org/C80444323","wikidata":"https://www.wikidata.org/wiki/Q2878974","display_name":"Theoretical computer science","level":1,"score":0.22006309032440186},{"id":"https://openalex.org/C192953774","wikidata":"https://www.wikidata.org/wiki/Q7307127","display_name":"Reference genome","level":4,"score":0.21797719597816467},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.10349652171134949},{"id":"https://openalex.org/C162317418","wikidata":"https://www.wikidata.org/wiki/Q252857","display_name":"Transcriptome","level":4,"score":0.0},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.0},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1007/s40484-019-0166-9","is_oa":true,"landing_page_url":"https://doi.org/10.1007/s40484-019-0166-9","pdf_url":"https://link.springer.com/content/pdf/10.1007/s40484-019-0166-9.pdf","source":{"id":"https://openalex.org/S4210172333","display_name":"Quantitative Biology","issn_l":"2095-4689","issn":["2095-4689","2095-4697"],"is_oa":true,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Quantitative Biology","raw_type":"journal-article"}],"best_oa_location":{"id":"doi:10.1007/s40484-019-0166-9","is_oa":true,"landing_page_url":"https://doi.org/10.1007/s40484-019-0166-9","pdf_url":"https://link.springer.com/content/pdf/10.1007/s40484-019-0166-9.pdf","source":{"id":"https://openalex.org/S4210172333","display_name":"Quantitative Biology","issn_l":"2095-4689","issn":["2095-4689","2095-4697"],"is_oa":true,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Quantitative Biology","raw_type":"journal-article"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/9","score":0.5299999713897705,"display_name":"Industry, innovation and infrastructure"}],"awards":[{"id":"https://openalex.org/G1187543282","display_name":null,"funder_award_id":"61772557","funder_id":"https://openalex.org/F4320321001","funder_display_name":"National Natural Science Foundation of China"},{"id":"https://openalex.org/G1474490597","display_name":null,"funder_award_id":"111 Project","funder_id":"https://openalex.org/F4320321001","funder_display_name":"National Natural Science Foundation of China"},{"id":"https://openalex.org/G1729077721","display_name":null,"funder_award_id":"61732009","funder_id":"https://openalex.org/F4320321001","funder_display_name":"National Natural Science Foundation of China"},{"id":"https://openalex.org/G2087396116","display_name":null,"funder_award_id":"China","funder_id":"https://openalex.org/F4320321001","funder_display_name":"National Natural Science Foundation of China"},{"id":"https://openalex.org/G2376276132","display_name":null,"funder_award_id":"China","funder_id":"https://openalex.org/F4320335787","funder_display_name":"Fundamental Research Funds for the Central Universities"},{"id":"https://openalex.org/G3317480652","display_name":null,"funder_award_id":"Science","funder_id":"https://openalex.org/F4320321001","funder_display_name":"National Natural Science Foundation of China"},{"id":"https://openalex.org/G358564744","display_name":null,"funder_award_id":"61420106009","funder_id":"https://openalex.org/F4320321001","funder_display_name":"National Natural Science Foundation of China"},{"id":"https://openalex.org/G4020255992","display_name":null,"funder_award_id":"Project","funder_id":"https://openalex.org/F4320321001","funder_display_name":"National Natural Science Foundation of China"},{"id":"https://openalex.org/G4066238608","display_name":null,"funder_award_id":"B18059","funder_id":"https://openalex.org/F4320327912","funder_display_name":"Higher Education Discipline Innovation Project"},{"id":"https://openalex.org/G5088138837","display_name":null,"funder_award_id":"B18059","funder_id":"https://openalex.org/F4320335785","funder_display_name":"Project 211"},{"id":"https://openalex.org/G5167091242","display_name":null,"funder_award_id":"No. 1","funder_id":"https://openalex.org/F4320321001","funder_display_name":"National Natural Science Foundation of China"},{"id":"https://openalex.org/G535964538","display_name":null,"funder_award_id":"2017117","funder_id":"https://openalex.org/F4320321001","funder_display_name":"National Natural Science Foundation of China"},{"id":"https://openalex.org/G5706847062","display_name":null,"funder_award_id":"6142010600","funder_id":"https://openalex.org/F4320321001","funder_display_name":"National Natural Science Foundation of China"},{"id":"https://openalex.org/G5994120800","display_name":null,"funder_award_id":"Natural","funder_id":"https://openalex.org/F4320321001","funder_display_name":"National Natural Science Foundation of China"},{"id":"https://openalex.org/G6541504238","display_name":null,"funder_award_id":"6177255","funder_id":"https://openalex.org/F4320321001","funder_display_name":"National Natural Science Foundation of China"},{"id":"https://openalex.org/G7631435625","display_name":null,"funder_award_id":"B18059","funder_id":"https://openalex.org/F4320321001","funder_display_name":"National Natural Science Foundation of China"},{"id":"https://openalex.org/G8197908271","display_name":null,"funder_award_id":"1053320171177","funder_id":"https://openalex.org/F4320335787","funder_display_name":"Fundamental Research Funds for the Central Universities"}],"funders":[{"id":"https://openalex.org/F4320321001","display_name":"National Natural Science Foundation of China","ror":"https://ror.org/01h0zpd94"},{"id":"https://openalex.org/F4320321514","display_name":"Central South University","ror":"https://ror.org/00f1zfq44"},{"id":"https://openalex.org/F4320327912","display_name":"Higher Education Discipline Innovation Project","ror":null},{"id":"https://openalex.org/F4320335785","display_name":"Project 211","ror":null},{"id":"https://openalex.org/F4320335787","display_name":"Fundamental Research Funds for the Central Universities","ror":null}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2941874147.pdf","grobid_xml":"https://content.openalex.org/works/W2941874147.grobid-xml"},"referenced_works_count":124,"referenced_works":["https://openalex.org/W33133400","https://openalex.org/W1490430289","https://openalex.org/W1550594300","https://openalex.org/W1579534339","https://openalex.org/W1590240800","https://openalex.org/W1603046567","https://openalex.org/W1635391495","https://openalex.org/W1793987388","https://openalex.org/W1903088142","https://openalex.org/W1966822396","https://openalex.org/W1968458420","https://openalex.org/W1972924519","https://openalex.org/W1974640383","https://openalex.org/W1980667059","https://openalex.org/W1982579644","https://openalex.org/W1983403167","https://openalex.org/W1984971730","https://openalex.org/W1988044297","https://openalex.org/W1992950355","https://openalex.org/W1997246534","https://openalex.org/W2005129098","https://openalex.org/W2010002522","https://openalex.org/W2011246380","https://openalex.org/W2013378764","https://openalex.org/W2014286596","https://openalex.org/W2022316530","https://openalex.org/W2022910024","https://openalex.org/W2022986961","https://openalex.org/W2023911006","https://openalex.org/W2041391522","https://openalex.org/W2046137036","https://openalex.org/W2050557699","https://openalex.org/W2051541780","https://openalex.org/W2058507386","https://openalex.org/W2059529172","https://openalex.org/W2064676287","https://openalex.org/W2066827579","https://openalex.org/W2073633042","https://openalex.org/W2075013491","https://openalex.org/W2076163156","https://openalex.org/W2082825869","https://openalex.org/W2084821192","https://openalex.org/W2101250487","https://openalex.org/W2103441770","https://openalex.org/W2105006906","https://openalex.org/W2105067980","https://openalex.org/W2105368763","https://openalex.org/W2106763286","https://openalex.org/W2107282968","https://openalex.org/W2110145781","https://openalex.org/W2113287691","https://openalex.org/W2115501618","https://openalex.org/W2117383593","https://openalex.org/W2118526609","https://openalex.org/W2119745866","https://openalex.org/W2120902911","https://openalex.org/W2121364424","https://openalex.org/W2121530737","https://openalex.org/W2124715997","https://openalex.org/W2124985265","https://openalex.org/W2128114769","https://openalex.org/W2129933858","https://openalex.org/W2130530163","https://openalex.org/W2131813051","https://openalex.org/W2133531097","https://openalex.org/W2133956160","https://openalex.org/W2136245516","https://openalex.org/W2136303928","https://openalex.org/W2136651963","https://openalex.org/W2137759177","https://openalex.org/W2138398659","https://openalex.org/W2140872496","https://openalex.org/W2141217788","https://openalex.org/W2141637710","https://openalex.org/W2141920662","https://openalex.org/W2142619120","https://openalex.org/W2142749416","https://openalex.org/W2144662851","https://openalex.org/W2149770991","https://openalex.org/W2150081252","https://openalex.org/W2151017710","https://openalex.org/W2154420975","https://openalex.org/W2154515562","https://openalex.org/W2160177274","https://openalex.org/W2160265768","https://openalex.org/W2160665949","https://openalex.org/W2160969485","https://openalex.org/W2161048357","https://openalex.org/W2161546116","https://openalex.org/W2163415691","https://openalex.org/W2167142288","https://openalex.org/W2167142762","https://openalex.org/W2167469524","https://openalex.org/W2167757586","https://openalex.org/W2176699305","https://openalex.org/W2179507357","https://openalex.org/W2180318265","https://openalex.org/W2189047836","https://openalex.org/W2194172909","https://openalex.org/W2195724570","https://openalex.org/W2211504288","https://openalex.org/W2269040222","https://openalex.org/W2330617919","https://openalex.org/W2345920351","https://openalex.org/W2383775397","https://openalex.org/W2396849069","https://openalex.org/W2397469828","https://openalex.org/W2399830453","https://openalex.org/W2520464109","https://openalex.org/W2537565528","https://openalex.org/W2579018450","https://openalex.org/W2580375537","https://openalex.org/W2610924493","https://openalex.org/W2782397389","https://openalex.org/W2886623299","https://openalex.org/W2890720538","https://openalex.org/W2897603144","https://openalex.org/W2951239321","https://openalex.org/W2952134619","https://openalex.org/W3099661149","https://openalex.org/W4231539689","https://openalex.org/W4248755869","https://openalex.org/W6601366811","https://openalex.org/W6677951329"],"related_works":["https://openalex.org/W3179696020","https://openalex.org/W4297088614","https://openalex.org/W2995491352","https://openalex.org/W280975","https://openalex.org/W1978814852","https://openalex.org/W3149677136","https://openalex.org/W2141920662","https://openalex.org/W2119121014","https://openalex.org/W2131813051","https://openalex.org/W1995663053"],"abstract_inverted_index":{"Background":[0],"Next\u2010generation":[1],"sequencing":[2,12,83,140,143,148,198],"(NGS)":[3],"technologies":[4],"have":[5,45],"fostered":[6],"an":[7,173],"unprecedented":[8],"proliferation":[9],"of":[10,18,24,67,81,114,133,137,156,175],"high\u2010throughput":[11],"projects":[13],"and":[14,43,55,85,107,110,118,145,179],"a":[15,195],"concomitant":[16],"development":[17],"novel":[19],"algorithms":[20],"for":[21,194],"the":[22,50,68,79,112,127,130,154,176,190],"assembly":[23,36,89,116,136,182,192],"short":[25],"reads.":[26],"However,":[27],"numerous":[28],"technical":[29],"or":[30],"computational":[31,56],"challenges":[32,51,70,132],"in":[33,52,125,160,164,181],"de":[34,95,104,134],"novo":[35,96,135],"still":[37],"remain,":[38],"although":[39],"many":[40],"new":[41],"ideas":[42],"solutions":[44,128],"been":[46],"suggested":[47],"to":[48,99,129,188],"tackle":[49],"both":[53],"experimental":[54],"settings.":[57],"Results":[58],"In":[59],"this":[60],"review,":[61],"we":[62,77,93,123,152],"first":[63],"briefly":[64],"introduce":[65,111,124],"some":[66],"major":[69],"faced":[71],"by":[72],"NGS":[73,165],"sequence":[74],"assembly.":[75,166],"Then,":[76],"analyze":[78],"characteristics":[80,113],"various":[82],"platforms":[84],"their":[86,100,119],"impact":[87],"on":[88],"results.":[90],"After":[91],"that,":[92],"classify":[94],"assemblers":[97],"according":[98],"frameworks":[101],"(overlap":[102],"graph\u2010based,":[103],"Bruijn":[105],"graph\u2010based":[106],"string":[108],"graph\u2010based),":[109],"each":[115],"tool":[117],"adaptation":[120],"scene.":[121],"Next,":[122],"detail":[126],"main":[131],"next":[138],"generation":[139],"data,":[141],"single\u2010cell":[142],"data":[144,199],"single":[146],"molecule":[147],"data.":[149],"At":[150],"last,":[151],"discuss":[153],"application":[155],"SMS":[157],"long":[158],"reads":[159],"solving":[161],"problems":[162],"encountered":[163],"Conclusions":[167],"This":[168],"review":[169],"not":[170],"only":[171],"gives":[172],"overview":[174],"latest":[177],"methods":[178],"developments":[180],"algorithms,":[183],"but":[184],"also":[185],"provides":[186],"guidelines":[187],"determine":[189],"optimal":[191],"algorithm":[193],"given":[196],"input":[197],"type.":[200]},"counts_by_year":[{"year":2026,"cited_by_count":4},{"year":2025,"cited_by_count":28},{"year":2024,"cited_by_count":16},{"year":2023,"cited_by_count":13},{"year":2022,"cited_by_count":13},{"year":2021,"cited_by_count":12},{"year":2020,"cited_by_count":4}],"updated_date":"2026-04-02T15:55:50.835912","created_date":"2025-10-10T00:00:00"}