{"id":"https://openalex.org/W1823385946","doi":"https://doi.org/10.1007/s40484-015-0046-x","title":"Rapid identification of multi\u2010strain HBV infection in patient by high\u2010throughput DNA sequencing","display_name":"Rapid identification of multi\u2010strain HBV infection in patient by high\u2010throughput DNA sequencing","publication_year":2015,"publication_date":"2015-06-15","ids":{"openalex":"https://openalex.org/W1823385946","doi":"https://doi.org/10.1007/s40484-015-0046-x","mag":"1823385946"},"language":"en","primary_location":{"id":"doi:10.1007/s40484-015-0046-x","is_oa":true,"landing_page_url":"https://doi.org/10.1007/s40484-015-0046-x","pdf_url":"https://journal.hep.com.cn/qb/EN/PDF/10.1007/s40484-015-0046-x","source":{"id":"https://openalex.org/S4210172333","display_name":"Quantitative Biology","issn_l":"2095-4689","issn":["2095-4689","2095-4697"],"is_oa":true,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Quantitative Biology","raw_type":"journal-article"},"type":"article","indexed_in":["crossref","doaj"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://journal.hep.com.cn/qb/EN/PDF/10.1007/s40484-015-0046-x","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5017277770","display_name":"Yongchang Zheng","orcid":"https://orcid.org/0000-0002-5916-2392"},"institutions":[{"id":"https://openalex.org/I200296433","display_name":"Chinese Academy of Medical Sciences & Peking Union Medical College","ror":"https://ror.org/02drdmm93","country_code":"CN","type":"education","lineage":["https://openalex.org/I200296433"]},{"id":"https://openalex.org/I2801228662","display_name":"Peking Union Medical College Hospital","ror":"https://ror.org/04jztag35","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I2801228662"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Yongchang Zheng","raw_affiliation_strings":["<!--1--> Department of Liver Surgery Peking Union Medical College Hospital Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100730 China","Department of Liver Surgery Peking Union Medical College Hospital Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100730 China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"<!--1--> Department of Liver Surgery Peking Union Medical College Hospital Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100730 China","institution_ids":["https://openalex.org/I200296433","https://openalex.org/I2801228662"]},{"raw_affiliation_string":"Department of Liver Surgery Peking Union Medical College Hospital Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100730 China","institution_ids":["https://openalex.org/I200296433","https://openalex.org/I2801228662"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5076103542","display_name":"Masood Ur Rehman Kayani","orcid":"https://orcid.org/0000-0002-7425-1756"},"institutions":[{"id":"https://openalex.org/I4210160507","display_name":"Center for Life Sciences","ror":"https://ror.org/05kje8j93","country_code":"CN","type":"education","lineage":["https://openalex.org/I20231570","https://openalex.org/I4210160507","https://openalex.org/I99065089"]},{"id":"https://openalex.org/I99065089","display_name":"Tsinghua University","ror":"https://ror.org/03cve4549","country_code":"CN","type":"education","lineage":["https://openalex.org/I99065089"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Masood ur Rehman Kayani","raw_affiliation_strings":["<!--2--> School of Life Sciences Center for Synthetic and Systems Biology MOE Key Laboratory of Bioinformatics Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases Tsinghua University Beijing 100084 China","School of Life Sciences Center for Synthetic and Systems Biology MOE Key Laboratory of Bioinformatics Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases Tsinghua University Beijing 100084 China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"<!--2--> School of Life Sciences Center for Synthetic and Systems Biology MOE Key Laboratory of Bioinformatics Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases Tsinghua University Beijing 100084 China","institution_ids":["https://openalex.org/I4210160507","https://openalex.org/I99065089"]},{"raw_affiliation_string":"School of Life Sciences Center for Synthetic and Systems Biology MOE Key Laboratory of Bioinformatics Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases Tsinghua University Beijing 100084 China","institution_ids":["https://openalex.org/I4210160507"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5081361043","display_name":"Ting Zhu","orcid":"https://orcid.org/0000-0003-0897-0303"},"institutions":[{"id":"https://openalex.org/I4210160507","display_name":"Center for Life Sciences","ror":"https://ror.org/05kje8j93","country_code":"CN","type":"education","lineage":["https://openalex.org/I20231570","https://openalex.org/I4210160507","https://openalex.org/I99065089"]},{"id":"https://openalex.org/I99065089","display_name":"Tsinghua University","ror":"https://ror.org/03cve4549","country_code":"CN","type":"education","lineage":["https://openalex.org/I99065089"]}],"countries":["CN"],"is_corresponding":true,"raw_author_name":"Ting F. Zhu","raw_affiliation_strings":["<!--2--> School of Life Sciences Center for Synthetic and Systems Biology MOE Key Laboratory of Bioinformatics Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases Tsinghua University Beijing 100084 China","School of Life Sciences Center for Synthetic and Systems Biology MOE Key Laboratory of Bioinformatics Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases Tsinghua University Beijing 100084 China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"<!--2--> School of Life Sciences Center for Synthetic and Systems Biology MOE Key Laboratory of Bioinformatics Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases Tsinghua University Beijing 100084 China","institution_ids":["https://openalex.org/I4210160507","https://openalex.org/I99065089"]},{"raw_affiliation_string":"School of Life Sciences Center for Synthetic and Systems Biology MOE Key Laboratory of Bioinformatics Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases Tsinghua University Beijing 100084 China","institution_ids":["https://openalex.org/I4210160507"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":4,"corresponding_author_ids":["https://openalex.org/A5081361043"],"corresponding_institution_ids":["https://openalex.org/I4210160507","https://openalex.org/I99065089"],"apc_list":{"value":1990,"currency":"USD","value_usd":1990},"apc_paid":{"value":1990,"currency":"USD","value_usd":1990},"fwci":0.0,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":{"value":0.04051105,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"3","issue":"2","first_page":"103","last_page":"106"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10340","display_name":"Hepatitis B Virus Studies","score":1.0,"subfield":{"id":"https://openalex.org/subfields/2713","display_name":"Epidemiology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10340","display_name":"Hepatitis B Virus Studies","score":1.0,"subfield":{"id":"https://openalex.org/subfields/2713","display_name":"Epidemiology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10151","display_name":"Hepatitis C virus research","score":0.9998000264167786,"subfield":{"id":"https://openalex.org/subfields/2721","display_name":"Hepatology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10351","display_name":"Liver Disease Diagnosis and Treatment","score":0.9983000159263611,"subfield":{"id":"https://openalex.org/subfields/2713","display_name":"Epidemiology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/hepatitis-b-virus","display_name":"Hepatitis B virus","score":0.6917046308517456},{"id":"https://openalex.org/keywords/virology","display_name":"Virology","score":0.6717811822891235},{"id":"https://openalex.org/keywords/strain","display_name":"Strain (injury)","score":0.5728917121887207},{"id":"https://openalex.org/keywords/identification","display_name":"Identification (biology)","score":0.5509289503097534},{"id":"https://openalex.org/keywords/dna-sequencing","display_name":"DNA sequencing","score":0.4706277847290039},{"id":"https://openalex.org/keywords/genome","display_name":"Genome","score":0.4620487093925476},{"id":"https://openalex.org/keywords/hepatitis-b","display_name":"Hepatitis B","score":0.4275064170360565},{"id":"https://openalex.org/keywords/biology","display_name":"Biology","score":0.41781121492385864},{"id":"https://openalex.org/keywords/deep-sequencing","display_name":"Deep sequencing","score":0.4158661961555481},{"id":"https://openalex.org/keywords/virus","display_name":"Virus","score":0.38633379340171814},{"id":"https://openalex.org/keywords/medicine","display_name":"Medicine","score":0.3691387176513672},{"id":"https://openalex.org/keywords/dna","display_name":"DNA","score":0.34053146839141846},{"id":"https://openalex.org/keywords/computational-biology","display_name":"Computational biology","score":0.32837483286857605},{"id":"https://openalex.org/keywords/gene","display_name":"Gene","score":0.2183925211429596},{"id":"https://openalex.org/keywords/genetics","display_name":"Genetics","score":0.11836287379264832},{"id":"https://openalex.org/keywords/internal-medicine","display_name":"Internal medicine","score":0.07902687788009644}],"concepts":[{"id":"https://openalex.org/C2780593183","wikidata":"https://www.wikidata.org/wiki/Q6844","display_name":"Hepatitis B virus","level":3,"score":0.6917046308517456},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.6717811822891235},{"id":"https://openalex.org/C2778022156","wikidata":"https://www.wikidata.org/wiki/Q576145","display_name":"Strain (injury)","level":2,"score":0.5728917121887207},{"id":"https://openalex.org/C116834253","wikidata":"https://www.wikidata.org/wiki/Q2039217","display_name":"Identification (biology)","level":2,"score":0.5509289503097534},{"id":"https://openalex.org/C51679486","wikidata":"https://www.wikidata.org/wiki/Q380546","display_name":"DNA sequencing","level":3,"score":0.4706277847290039},{"id":"https://openalex.org/C141231307","wikidata":"https://www.wikidata.org/wiki/Q7020","display_name":"Genome","level":3,"score":0.4620487093925476},{"id":"https://openalex.org/C2777382497","wikidata":"https://www.wikidata.org/wiki/Q6853","display_name":"Hepatitis B","level":2,"score":0.4275064170360565},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.41781121492385864},{"id":"https://openalex.org/C132917006","wikidata":"https://www.wikidata.org/wiki/Q5250436","display_name":"Deep sequencing","level":4,"score":0.4158661961555481},{"id":"https://openalex.org/C2522874641","wikidata":"https://www.wikidata.org/wiki/Q808","display_name":"Virus","level":2,"score":0.38633379340171814},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.3691387176513672},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.34053146839141846},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.32837483286857605},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.2183925211429596},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.11836287379264832},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.07902687788009644},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1007/s40484-015-0046-x","is_oa":true,"landing_page_url":"https://doi.org/10.1007/s40484-015-0046-x","pdf_url":"https://journal.hep.com.cn/qb/EN/PDF/10.1007/s40484-015-0046-x","source":{"id":"https://openalex.org/S4210172333","display_name":"Quantitative Biology","issn_l":"2095-4689","issn":["2095-4689","2095-4697"],"is_oa":true,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Quantitative Biology","raw_type":"journal-article"}],"best_oa_location":{"id":"doi:10.1007/s40484-015-0046-x","is_oa":true,"landing_page_url":"https://doi.org/10.1007/s40484-015-0046-x","pdf_url":"https://journal.hep.com.cn/qb/EN/PDF/10.1007/s40484-015-0046-x","source":{"id":"https://openalex.org/S4210172333","display_name":"Quantitative Biology","issn_l":"2095-4689","issn":["2095-4689","2095-4697"],"is_oa":true,"is_in_doaj":true,"is_core":true,"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Quantitative Biology","raw_type":"journal-article"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.8700000047683716,"display_name":"Good health and well-being"}],"awards":[{"id":"https://openalex.org/G2567331818","display_name":"\u5927\u6c14\u53ef\u5438\u5165\u9897\u7c92\u7269\u4e2d\u5fae\u751f\u7269\u79cd\u7fa4\u52a8\u6001\u53d8\u5316\u53ca\u6297\u836f\u529f\u80fd\u57fa\u56e0\u7ec4\u7814\u7a76","funder_award_id":"31470532","funder_id":"https://openalex.org/F4320321001","funder_display_name":"National Natural Science Foundation of China"},{"id":"https://openalex.org/G430146206","display_name":null,"funder_award_id":"2015CB553402","funder_id":"https://openalex.org/F4320335777","funder_display_name":"National Key Research and Development Program of China"}],"funders":[{"id":"https://openalex.org/F4320321001","display_name":"National Natural Science Foundation of China","ror":"https://ror.org/01h0zpd94"},{"id":"https://openalex.org/F4320322392","display_name":"Tsinghua University","ror":"https://ror.org/03cve4549"},{"id":"https://openalex.org/F4320324787","display_name":"Peking University","ror":"https://ror.org/02v51f717"},{"id":"https://openalex.org/F4320335777","display_name":"National Key Research and Development Program of China","ror":null}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W1823385946.pdf","grobid_xml":"https://content.openalex.org/works/W1823385946.grobid-xml"},"referenced_works_count":17,"referenced_works":["https://openalex.org/W1502620477","https://openalex.org/W1880969726","https://openalex.org/W1964165326","https://openalex.org/W1972186760","https://openalex.org/W1983420763","https://openalex.org/W2013054809","https://openalex.org/W2021983329","https://openalex.org/W2038979436","https://openalex.org/W2046848640","https://openalex.org/W2046892851","https://openalex.org/W2082711035","https://openalex.org/W2083084779","https://openalex.org/W2122440054","https://openalex.org/W2126216210","https://openalex.org/W2126494596","https://openalex.org/W2130750738","https://openalex.org/W2156089124"],"related_works":["https://openalex.org/W2040973434","https://openalex.org/W1969673184","https://openalex.org/W2362454490","https://openalex.org/W3132115146","https://openalex.org/W2619166255","https://openalex.org/W2472738690","https://openalex.org/W1539994441","https://openalex.org/W3011303654","https://openalex.org/W2074464258","https://openalex.org/W2359210733"],"abstract_inverted_index":{"Hepatitis":[0],"B":[1,81],"is":[2,14,39],"a":[3,43,58,109],"well\u2010known":[4],"risk":[5],"factor":[6],"for":[7,45,75],"the":[8,27,46,100,115,125],"development":[9],"of":[10,49,65,78,95,130],"liver":[11],"cancer":[12],"and":[13,20,24,33,36,68,127,134],"closely":[15],"associated":[16],"with":[17,91],"patient":[18],"morbidity":[19],"mortality.":[21],"Viral":[22],"mutants":[23],"variants":[25],"have":[26],"potential":[28],"to":[29,41,113,124],"evade":[30],"immune":[31],"response":[32],"prolong":[34],"infection,":[35],"thus":[37],"it":[38],"crucial":[40],"develop":[42],"methodology":[44],"rapid":[47,76,126],"identification":[48,77,129],"multi\u2010strain":[50,79,131],"hepatitis":[51,80],"infections":[52],"in":[53,85],"patients.":[54,86],"Here":[55],"we":[56],"describe":[57],"method":[59,88,119],"based":[60],"on":[61],"selective":[62],"region":[63],"amplification":[64],"viral":[66],"genome":[67],"deep":[69],"sequencing,":[70],"which":[71],"may":[72],"be":[73,122],"used":[74],"virus":[82],"(HBV)":[83],"infection":[84,133],"The":[87],"works":[89],"even":[90],"significantly":[92],"low":[93],"amounts":[94],"patients\u2019":[96],"serum":[97],"samples,":[98],"where":[99],"wet\u2010lab":[101],"procedures":[102],"take":[103],"about":[104],"1.5":[105],"days,":[106],"followed":[107],"by":[108],"quick":[110],"bioinformatic":[111],"analysis":[112],"reveal":[114],"final":[116],"results.":[117],"Our":[118],"can":[120],"potentially":[121],"applied":[123],"reliable":[128],"HBV":[132],"help":[135],"improve":[136],"treatment":[137],"regiments.":[138]},"counts_by_year":[],"updated_date":"2026-07-23T08:03:31.855105","created_date":"2025-10-10T00:00:00"}