{"id":"https://openalex.org/W2414769155","doi":"https://doi.org/10.1007/978-1-61779-885-6_12","title":"Hydroponic Isotope Labeling of Entire Plants and High-Performance Mass Spectrometry for Quantitative Plant Proteomics","display_name":"Hydroponic Isotope Labeling of Entire Plants and High-Performance Mass Spectrometry for Quantitative Plant Proteomics","publication_year":2012,"publication_date":"2012-01-01","ids":{"openalex":"https://openalex.org/W2414769155","doi":"https://doi.org/10.1007/978-1-61779-885-6_12","mag":"2414769155","pmid":"https://pubmed.ncbi.nlm.nih.gov/22665301"},"language":"en","primary_location":{"id":"doi:10.1007/978-1-61779-885-6_12","is_oa":false,"landing_page_url":"https://doi.org/10.1007/978-1-61779-885-6_12","pdf_url":null,"source":{"id":"https://openalex.org/S4210172139","display_name":"Methods in molecular biology","issn_l":"1064-3745","issn":["1064-3745","1940-6029"],"is_oa":false,"is_in_doaj":false,"is_core":true,"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"book series"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in Molecular Biology","raw_type":"book-chapter"},"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5071400050","display_name":"Laurence V. Bindschedler","orcid":"https://orcid.org/0000-0002-0421-9857"},"institutions":[{"id":"https://openalex.org/I71052956","display_name":"University of Reading","ror":"https://ror.org/05v62cm79","country_code":"GB","type":"education","lineage":["https://openalex.org/I71052956"]}],"countries":["GB"],"is_corresponding":true,"raw_author_name":"Laurence V. Bindschedler","raw_affiliation_strings":["Department of Chemistry, University of Reading, Reading, UK. l.v.bindschedler@reading.ac.uk","Department of Chemistry, University of Reading, Reading, UK"],"affiliations":[{"raw_affiliation_string":"Department of Chemistry, University of Reading, Reading, UK. l.v.bindschedler@reading.ac.uk","institution_ids":["https://openalex.org/I71052956"]},{"raw_affiliation_string":"Department of Chemistry, University of Reading, Reading, UK","institution_ids":["https://openalex.org/I71052956"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5056423343","display_name":"Davinia J. Mills","orcid":null},"institutions":[{"id":"https://openalex.org/I71052956","display_name":"University of Reading","ror":"https://ror.org/05v62cm79","country_code":"GB","type":"education","lineage":["https://openalex.org/I71052956"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Davinia J. S. Mills","raw_affiliation_strings":["The BioCentre, University of Reading, Reading, UK"],"affiliations":[{"raw_affiliation_string":"The BioCentre, University of Reading, Reading, UK","institution_ids":["https://openalex.org/I71052956"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5016772331","display_name":"Rainer Cramer","orcid":"https://orcid.org/0000-0002-8037-2511"},"institutions":[{"id":"https://openalex.org/I71052956","display_name":"University of Reading","ror":"https://ror.org/05v62cm79","country_code":"GB","type":"education","lineage":["https://openalex.org/I71052956"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"Rainer Cramer","raw_affiliation_strings":["Department of Chemistry, University of Reading, Reading, UK","University of Reading, Reading, UK"],"affiliations":[{"raw_affiliation_string":"Department of Chemistry, University of Reading, Reading, UK","institution_ids":["https://openalex.org/I71052956"]},{"raw_affiliation_string":"University of Reading, Reading, UK","institution_ids":["https://openalex.org/I71052956"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5071400050"],"corresponding_institution_ids":["https://openalex.org/I71052956"],"apc_list":null,"apc_paid":null,"fwci":2.7048,"has_fulltext":false,"cited_by_count":4,"citation_normalized_percentile":{"value":0.89934211,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":96},"biblio":{"volume":"893","issue":null,"first_page":"155","last_page":"173"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10519","display_name":"Advanced Proteomics Techniques and Applications","score":0.9998999834060669,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T10519","display_name":"Advanced Proteomics Techniques and Applications","score":0.9998999834060669,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10683","display_name":"Mass Spectrometry Techniques and Applications","score":0.9979000091552734,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10836","display_name":"Metabolomics and Mass Spectrometry Studies","score":0.9868999719619751,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/chemistry","display_name":"Chemistry","score":0.7248095870018005},{"id":"https://openalex.org/keywords/shotgun-proteomics","display_name":"Shotgun proteomics","score":0.714523196220398},{"id":"https://openalex.org/keywords/mass-spectrometry","display_name":"Mass spectrometry","score":0.6993895769119263},{"id":"https://openalex.org/keywords/proteome","display_name":"Proteome","score":0.6659103631973267},{"id":"https://openalex.org/keywords/proteomics","display_name":"Proteomics","score":0.614952802658081},{"id":"https://openalex.org/keywords/chromatography","display_name":"Chromatography","score":0.6022493839263916},{"id":"https://openalex.org/keywords/quantitative-proteomics","display_name":"Quantitative proteomics","score":0.5671571493148804},{"id":"https://openalex.org/keywords/isobaric-labeling","display_name":"Isobaric labeling","score":0.561803936958313},{"id":"https://openalex.org/keywords/stable-isotope-ratio","display_name":"Stable isotope ratio","score":0.4927387535572052},{"id":"https://openalex.org/keywords/tandem-mass-spectrometry","display_name":"Tandem mass spectrometry","score":0.4923757314682007},{"id":"https://openalex.org/keywords/tandem-mass-tag","display_name":"Tandem mass tag","score":0.47583436965942383},{"id":"https://openalex.org/keywords/label-free-quantification","display_name":"Label-free quantification","score":0.4200286865234375},{"id":"https://openalex.org/keywords/protein-mass-spectrometry","display_name":"Protein mass spectrometry","score":0.3110082149505615},{"id":"https://openalex.org/keywords/biochemistry","display_name":"Biochemistry","score":0.1694488227367401}],"concepts":[{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.7248095870018005},{"id":"https://openalex.org/C68289359","wikidata":"https://www.wikidata.org/wiki/Q7502514","display_name":"Shotgun proteomics","level":4,"score":0.714523196220398},{"id":"https://openalex.org/C162356407","wikidata":"https://www.wikidata.org/wiki/Q180809","display_name":"Mass spectrometry","level":2,"score":0.6993895769119263},{"id":"https://openalex.org/C104397665","wikidata":"https://www.wikidata.org/wiki/Q860947","display_name":"Proteome","level":2,"score":0.6659103631973267},{"id":"https://openalex.org/C46111723","wikidata":"https://www.wikidata.org/wiki/Q471857","display_name":"Proteomics","level":3,"score":0.614952802658081},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.6022493839263916},{"id":"https://openalex.org/C80311884","wikidata":"https://www.wikidata.org/wiki/Q7268939","display_name":"Quantitative proteomics","level":4,"score":0.5671571493148804},{"id":"https://openalex.org/C184185385","wikidata":"https://www.wikidata.org/wiki/Q6085263","display_name":"Isobaric labeling","level":5,"score":0.561803936958313},{"id":"https://openalex.org/C22117777","wikidata":"https://www.wikidata.org/wiki/Q17148629","display_name":"Stable isotope ratio","level":2,"score":0.4927387535572052},{"id":"https://openalex.org/C31827203","wikidata":"https://www.wikidata.org/wiki/Q874296","display_name":"Tandem mass spectrometry","level":3,"score":0.4923757314682007},{"id":"https://openalex.org/C86153913","wikidata":"https://www.wikidata.org/wiki/Q7682439","display_name":"Tandem mass tag","level":5,"score":0.47583436965942383},{"id":"https://openalex.org/C73090800","wikidata":"https://www.wikidata.org/wiki/Q6466869","display_name":"Label-free quantification","level":5,"score":0.4200286865234375},{"id":"https://openalex.org/C202157981","wikidata":"https://www.wikidata.org/wiki/Q7251488","display_name":"Protein mass spectrometry","level":4,"score":0.3110082149505615},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.1694488227367401},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.0},{"id":"https://openalex.org/C62520636","wikidata":"https://www.wikidata.org/wiki/Q944","display_name":"Quantum mechanics","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D003627","descriptor_name":"Data Interpretation, Statistical","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003627","descriptor_name":"Data Interpretation, Statistical","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003627","descriptor_name":"Data Interpretation, Statistical","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003627","descriptor_name":"Data Interpretation, Statistical","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007553","descriptor_name":"Isotope Labeling","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007553","descriptor_name":"Isotope Labeling","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007553","descriptor_name":"Isotope Labeling","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007553","descriptor_name":"Isotope Labeling","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009587","descriptor_name":"Nitrogen Isotopes","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009587","descriptor_name":"Nitrogen Isotopes","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009587","descriptor_name":"Nitrogen Isotopes","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009587","descriptor_name":"Nitrogen Isotopes","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010446","descriptor_name":"Peptide Fragments","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D010446","descriptor_name":"Peptide Fragments","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D010446","descriptor_name":"Peptide Fragments","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D010446","descriptor_name":"Peptide Fragments","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D010449","descriptor_name":"Peptide Mapping","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010449","descriptor_name":"Peptide Mapping","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010449","descriptor_name":"Peptide Mapping","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010449","descriptor_name":"Peptide Mapping","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012984","descriptor_name":"Software","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012984","descriptor_name":"Software","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012984","descriptor_name":"Software","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012984","descriptor_name":"Software","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014357","descriptor_name":"Trypsin","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D014357","descriptor_name":"Trypsin","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D014357","descriptor_name":"Trypsin","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D014357","descriptor_name":"Trypsin","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D017360","descriptor_name":"Arabidopsis","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D017360","descriptor_name":"Arabidopsis","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D017360","descriptor_name":"Arabidopsis","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D017360","descriptor_name":"Arabidopsis","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D017360","descriptor_name":"Arabidopsis","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D017360","descriptor_name":"Arabidopsis","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D017360","descriptor_name":"Arabidopsis","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D017360","descriptor_name":"Arabidopsis","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D018515","descriptor_name":"Plant Leaves","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D018515","descriptor_name":"Plant Leaves","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D018515","descriptor_name":"Plant Leaves","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D018515","descriptor_name":"Plant Leaves","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D018515","descriptor_name":"Plant Leaves","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D018515","descriptor_name":"Plant Leaves","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D018515","descriptor_name":"Plant Leaves","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D018515","descriptor_name":"Plant Leaves","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D018527","descriptor_name":"Hydroponics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D018527","descriptor_name":"Hydroponics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D018527","descriptor_name":"Hydroponics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D018527","descriptor_name":"Hydroponics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D020543","descriptor_name":"Proteome","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D020543","descriptor_name":"Proteome","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D020543","descriptor_name":"Proteome","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D020543","descriptor_name":"Proteome","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D020543","descriptor_name":"Proteome","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D020543","descriptor_name":"Proteome","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D020543","descriptor_name":"Proteome","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D020543","descriptor_name":"Proteome","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D020543","descriptor_name":"Proteome","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D020543","descriptor_name":"Proteome","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D020543","descriptor_name":"Proteome","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D020543","descriptor_name":"Proteome","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D021241","descriptor_name":"Spectrometry, Mass, Electrospray Ionization","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D021241","descriptor_name":"Spectrometry, Mass, Electrospray Ionization","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D021241","descriptor_name":"Spectrometry, Mass, Electrospray Ionization","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D021241","descriptor_name":"Spectrometry, Mass, Electrospray Ionization","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D029681","descriptor_name":"Arabidopsis Proteins","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D029681","descriptor_name":"Arabidopsis Proteins","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D029681","descriptor_name":"Arabidopsis Proteins","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D029681","descriptor_name":"Arabidopsis Proteins","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D029681","descriptor_name":"Arabidopsis Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D029681","descriptor_name":"Arabidopsis Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D029681","descriptor_name":"Arabidopsis Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D029681","descriptor_name":"Arabidopsis Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D029681","descriptor_name":"Arabidopsis Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D029681","descriptor_name":"Arabidopsis Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D029681","descriptor_name":"Arabidopsis Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D029681","descriptor_name":"Arabidopsis Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D040901","descriptor_name":"Proteomics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D040901","descriptor_name":"Proteomics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D040901","descriptor_name":"Proteomics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D040901","descriptor_name":"Proteomics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D046508","descriptor_name":"Culture Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D046508","descriptor_name":"Culture Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D046508","descriptor_name":"Culture Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D046508","descriptor_name":"Culture Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D053719","descriptor_name":"Tandem Mass Spectrometry","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D053719","descriptor_name":"Tandem Mass Spectrometry","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D053719","descriptor_name":"Tandem Mass Spectrometry","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D053719","descriptor_name":"Tandem Mass Spectrometry","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D056148","descriptor_name":"Chromatography, Reverse-Phase","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D056148","descriptor_name":"Chromatography, Reverse-Phase","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D056148","descriptor_name":"Chromatography, Reverse-Phase","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D056148","descriptor_name":"Chromatography, Reverse-Phase","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D059748","descriptor_name":"Proteolysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D059748","descriptor_name":"Proteolysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D059748","descriptor_name":"Proteolysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D059748","descriptor_name":"Proteolysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1007/978-1-61779-885-6_12","is_oa":false,"landing_page_url":"https://doi.org/10.1007/978-1-61779-885-6_12","pdf_url":null,"source":{"id":"https://openalex.org/S4210172139","display_name":"Methods in molecular biology","issn_l":"1064-3745","issn":["1064-3745","1940-6029"],"is_oa":false,"is_in_doaj":false,"is_core":true,"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"book series"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in Molecular Biology","raw_type":"book-chapter"},{"id":"pmid:22665301","is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/22665301","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in molecular biology (Clifton, N.J.)","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[],"awards":[{"id":"https://openalex.org/G2782497380","display_name":null,"funder_award_id":"BB/H001948/1","funder_id":"https://openalex.org/F4320334629","funder_display_name":"Biotechnology and Biological Sciences Research Council"}],"funders":[{"id":"https://openalex.org/F4320334629","display_name":"Biotechnology and Biological Sciences Research Council","ror":"https://ror.org/00cwqg982"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":28,"referenced_works":["https://openalex.org/W167421660","https://openalex.org/W1965463375","https://openalex.org/W1975558168","https://openalex.org/W1978866739","https://openalex.org/W1984365278","https://openalex.org/W1989636155","https://openalex.org/W1997898173","https://openalex.org/W2006986579","https://openalex.org/W2026250677","https://openalex.org/W2026805700","https://openalex.org/W2037334311","https://openalex.org/W2046726975","https://openalex.org/W2053139780","https://openalex.org/W2055155126","https://openalex.org/W2064592878","https://openalex.org/W2085233887","https://openalex.org/W2085529577","https://openalex.org/W2096550677","https://openalex.org/W2099682053","https://openalex.org/W2104763849","https://openalex.org/W2115934662","https://openalex.org/W2132899146","https://openalex.org/W2141018553","https://openalex.org/W2166390731","https://openalex.org/W2166793037","https://openalex.org/W2167542004","https://openalex.org/W2793216206","https://openalex.org/W2894517388"],"related_works":["https://openalex.org/W2570472928","https://openalex.org/W2157839186","https://openalex.org/W2436016506","https://openalex.org/W2971374468","https://openalex.org/W2465149832","https://openalex.org/W2062972748","https://openalex.org/W2041476285","https://openalex.org/W2067982561","https://openalex.org/W4200113212","https://openalex.org/W3212207955"],"abstract_inverted_index":{"Hydroponic":[0],"isotope":[1,203],"labeling":[2,13,204],"of":[3,41,146],"entire":[4],"plants":[5,43,62,200],"(HILEP)":[6],"combines":[7],"hydroponic":[8],"plant":[9,58],"cultivation":[10],"and":[11,24,71,94,129,138,149,151,157,166,208],"metabolic":[12],"with":[14,96,118,136,181],"stable":[15],"isotopes":[16],"using":[17,102,175,205,215],"(15)N-containing":[18],"inorganic":[19],"salts":[20,29,207],"to":[21,38,49,197,210],"label":[22],"whole":[23],"mature":[25],"plants.":[26],"Employing":[27],"(15)N":[28,46,206],"as":[30],"the":[31,39,74,132,144,187,212],"sole":[32],"nitrogen":[33],"source":[34],"for":[35,56,79,99,131,202],"HILEP":[36,53],"leads":[37],"production":[40],"healthy-looking":[42],"which":[44],"contain":[45],"proteins":[47,147],"labeled":[48],"nearly":[50],"100%.":[51],"Therefore,":[52],"is":[54],"suitable":[55,92],"quantitative":[57],"proteomic":[59],"analysis,":[60],"where":[61],"are":[63,77],"grown":[64],"in":[65,90,170],"either":[66],"(14)N-":[67],"or":[68],"(15)N-hydroponic":[69],"media":[70],"pooled":[72,84],"when":[73],"biological":[75],"samples":[76],"collected":[78],"relative":[80],"proteome":[81],"quantitation.":[82,154],"The":[83],"(14)N-/(15)N-protein":[85],"extracts":[86],"can":[87],"be":[88],"fractionated":[89],"any":[91],"way":[93,174],"digested":[95],"a":[97,119,171,216],"protease":[98],"shotgun":[100],"proteomics,":[101],"typically":[103],"reverse":[104],"phase":[105],"liquid":[106],"chromatography":[107],"nanoelectrospray":[108],"ionization":[109],"tandem":[110],"mass":[111,123,127],"spectrometry":[112],"(RPLC-nESI-MS/MS).":[113],"Best":[114],"results":[115],"were":[116,168],"obtained":[117],"hybrid":[120],"ion":[121],"trap/FT-MS":[122],"spectrometer,":[124],"combining":[125],"high":[126],"accuracy":[128],"sensitivity":[130],"MS":[133,161,177],"data":[134,141,162,178],"acquisition":[135],"speed":[137],"high-throughput":[139],"MS/MS":[140],"acquisition,":[142],"increasing":[143],"number":[145],"identified":[148],"quantified":[150],"improving":[152],"protein":[153,164],"Peak":[155],"processing":[156],"picking":[158],"from":[159],"raw":[160],"files,":[163],"identification,":[165],"quantitation":[167],"performed":[169],"highly":[172],"automated":[173],"integrated":[176],"analysis":[179],"software":[180],"minimum":[182],"manual":[183],"intervention,":[184],"thus":[185],"easing":[186],"analytical":[188],"workflow.":[189],"In":[190],"this":[191],"methodology":[192],"paper,":[193],"we":[194],"describe":[195],"how":[196,209],"grow":[198],"Arabidopsis":[199],"hydroponically":[201],"quantitate":[211],"resulting":[213],"proteomes":[214],"convenient":[217],"workflow":[218],"that":[219],"does":[220],"not":[221],"require":[222],"extensive":[223],"bioinformatics":[224],"skills.":[225]},"counts_by_year":[{"year":2016,"cited_by_count":1},{"year":2013,"cited_by_count":2},{"year":2012,"cited_by_count":1}],"updated_date":"2026-01-13T01:12:25.745995","created_date":"2025-10-10T00:00:00"}