{"id":"https://openalex.org/W72673129","doi":"https://doi.org/10.1007/978-1-60761-444-9_18","title":"An Overview of Label-Free Quantitation Methods in Proteomics by Mass Spectrometry","display_name":"An Overview of Label-Free Quantitation Methods in Proteomics by Mass Spectrometry","publication_year":2009,"publication_date":"2009-12-05","ids":{"openalex":"https://openalex.org/W72673129","doi":"https://doi.org/10.1007/978-1-60761-444-9_18","mag":"72673129","pmid":"https://pubmed.ncbi.nlm.nih.gov/20013377"},"language":"en","primary_location":{"id":"doi:10.1007/978-1-60761-444-9_18","is_oa":false,"landing_page_url":"https://doi.org/10.1007/978-1-60761-444-9_18","pdf_url":null,"source":{"id":"https://openalex.org/S4210172139","display_name":"Methods in molecular biology","issn_l":"1064-3745","issn":["1064-3745","1940-6029"],"is_oa":false,"is_in_doaj":false,"is_core":true,"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"book series"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in Molecular Biology","raw_type":"book-chapter"},"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5072309345","display_name":"Jason W.H. Wong","orcid":"https://orcid.org/0000-0003-2953-7728"},"institutions":[{"id":"https://openalex.org/I31746571","display_name":"UNSW Sydney","ror":"https://ror.org/03r8z3t63","country_code":"AU","type":"education","lineage":["https://openalex.org/I31746571"]}],"countries":["AU"],"is_corresponding":true,"raw_author_name":"Jason W. H. Wong","raw_affiliation_strings":["UNSW Cancer Research Centre, University of New South Wales, Sydney, NSW, Australia"],"affiliations":[{"raw_affiliation_string":"UNSW Cancer Research Centre, University of New South Wales, Sydney, NSW, Australia","institution_ids":["https://openalex.org/I31746571"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5057948821","display_name":"Gerard Cagney","orcid":"https://orcid.org/0000-0001-7189-9496"},"institutions":[{"id":"https://openalex.org/I100930933","display_name":"University College Dublin","ror":"https://ror.org/05m7pjf47","country_code":"IE","type":"education","lineage":["https://openalex.org/I100930933"]}],"countries":["IE"],"is_corresponding":false,"raw_author_name":"Gerard Cagney","raw_affiliation_strings":["Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Belfield, Dublin 4, Ireland"],"affiliations":[{"raw_affiliation_string":"Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Belfield, Dublin 4, Ireland","institution_ids":["https://openalex.org/I100930933"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5072309345"],"corresponding_institution_ids":["https://openalex.org/I31746571"],"apc_list":null,"apc_paid":null,"fwci":15.8525,"has_fulltext":false,"cited_by_count":128,"citation_normalized_percentile":{"value":0.99092971,"is_in_top_1_percent":true,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":95,"max":100},"biblio":{"volume":"604","issue":null,"first_page":"273","last_page":"283"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10519","display_name":"Advanced Proteomics Techniques and Applications","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T10519","display_name":"Advanced Proteomics Techniques and Applications","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10683","display_name":"Mass Spectrometry Techniques and Applications","score":0.9998000264167786,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10836","display_name":"Metabolomics and Mass Spectrometry Studies","score":0.9983000159263611,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/quantitative-proteomics","display_name":"Quantitative proteomics","score":0.8218885660171509},{"id":"https://openalex.org/keywords/proteomics","display_name":"Proteomics","score":0.796349048614502},{"id":"https://openalex.org/keywords/label-free-quantification","display_name":"Label-free quantification","score":0.7567324042320251},{"id":"https://openalex.org/keywords/mass-spectrometry","display_name":"Mass spectrometry","score":0.60568767786026},{"id":"https://openalex.org/keywords/identification","display_name":"Identification (biology)","score":0.4628531038761139},{"id":"https://openalex.org/keywords/computer-science","display_name":"Computer science","score":0.4498920142650604},{"id":"https://openalex.org/keywords/computational-biology","display_name":"Computational biology","score":0.43653666973114014},{"id":"https://openalex.org/keywords/chemistry","display_name":"Chemistry","score":0.3915906548500061},{"id":"https://openalex.org/keywords/chromatography","display_name":"Chromatography","score":0.37610068917274475},{"id":"https://openalex.org/keywords/biology","display_name":"Biology","score":0.14983224868774414},{"id":"https://openalex.org/keywords/biochemistry","display_name":"Biochemistry","score":0.07417887449264526}],"concepts":[{"id":"https://openalex.org/C80311884","wikidata":"https://www.wikidata.org/wiki/Q7268939","display_name":"Quantitative proteomics","level":4,"score":0.8218885660171509},{"id":"https://openalex.org/C46111723","wikidata":"https://www.wikidata.org/wiki/Q471857","display_name":"Proteomics","level":3,"score":0.796349048614502},{"id":"https://openalex.org/C73090800","wikidata":"https://www.wikidata.org/wiki/Q6466869","display_name":"Label-free quantification","level":5,"score":0.7567324042320251},{"id":"https://openalex.org/C162356407","wikidata":"https://www.wikidata.org/wiki/Q180809","display_name":"Mass spectrometry","level":2,"score":0.60568767786026},{"id":"https://openalex.org/C116834253","wikidata":"https://www.wikidata.org/wiki/Q2039217","display_name":"Identification (biology)","level":2,"score":0.4628531038761139},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.4498920142650604},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.43653666973114014},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3915906548500061},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.37610068917274475},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.14983224868774414},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.07417887449264526},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D002853","descriptor_name":"Chromatography, Liquid","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D002853","descriptor_name":"Chromatography, Liquid","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D002853","descriptor_name":"Chromatography, Liquid","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D007477","descriptor_name":"Ions","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D007477","descriptor_name":"Ions","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D007477","descriptor_name":"Ions","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D011506","descriptor_name":"Proteins","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D011506","descriptor_name":"Proteins","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D011506","descriptor_name":"Proteins","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D013058","descriptor_name":"Mass Spectrometry","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D013058","descriptor_name":"Mass Spectrometry","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D013058","descriptor_name":"Mass Spectrometry","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D040901","descriptor_name":"Proteomics","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D040901","descriptor_name":"Proteomics","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D040901","descriptor_name":"Proteomics","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1007/978-1-60761-444-9_18","is_oa":false,"landing_page_url":"https://doi.org/10.1007/978-1-60761-444-9_18","pdf_url":null,"source":{"id":"https://openalex.org/S4210172139","display_name":"Methods in molecular biology","issn_l":"1064-3745","issn":["1064-3745","1940-6029"],"is_oa":false,"is_in_doaj":false,"is_core":true,"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"book series"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in Molecular Biology","raw_type":"book-chapter"},{"id":"pmid:20013377","is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/20013377","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in molecular biology (Clifton, N.J.)","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":42,"referenced_works":["https://openalex.org/W1525575010","https://openalex.org/W1597678601","https://openalex.org/W1775749144","https://openalex.org/W1970696723","https://openalex.org/W1973281997","https://openalex.org/W1981593008","https://openalex.org/W1982411844","https://openalex.org/W1990815293","https://openalex.org/W1997504014","https://openalex.org/W2004287303","https://openalex.org/W2015556811","https://openalex.org/W2023096047","https://openalex.org/W2026465178","https://openalex.org/W2027961289","https://openalex.org/W2045234988","https://openalex.org/W2046733225","https://openalex.org/W2047275456","https://openalex.org/W2056467018","https://openalex.org/W2063543381","https://openalex.org/W2064543105","https://openalex.org/W2065436349","https://openalex.org/W2065720951","https://openalex.org/W2067074165","https://openalex.org/W2068481474","https://openalex.org/W2069862631","https://openalex.org/W2075036506","https://openalex.org/W2086216615","https://openalex.org/W2088697979","https://openalex.org/W2092418525","https://openalex.org/W2100702185","https://openalex.org/W2107892065","https://openalex.org/W2111208057","https://openalex.org/W2112078820","https://openalex.org/W2120016245","https://openalex.org/W2128635872","https://openalex.org/W2140695271","https://openalex.org/W2140946052","https://openalex.org/W2148342439","https://openalex.org/W2152180036","https://openalex.org/W2163457670","https://openalex.org/W2171757797","https://openalex.org/W4293247451"],"related_works":["https://openalex.org/W2944683884","https://openalex.org/W2160416873","https://openalex.org/W3081950544","https://openalex.org/W3089696757","https://openalex.org/W2000259163","https://openalex.org/W2014032285","https://openalex.org/W2374027747","https://openalex.org/W4376133284","https://openalex.org/W1574322319","https://openalex.org/W2181312330"],"abstract_inverted_index":{"Protein":[0],"quantification":[1,22,34],"represents":[2],"an":[3,43,51],"important":[4],"extension":[5],"to":[6,80],"identification":[7],"proteomics,":[8],"enabling":[9],"the":[10,54,70,82],"comparison":[11],"of":[12,35,53,76,84],"protein":[13,21],"expression":[14],"across":[15],"different":[16,55],"samples":[17],"or":[18],"treatments.":[19],"Comparative":[20],"by":[23],"mass":[24],"spectrometry":[25],"typically":[26],"employs":[27],"stable":[28],"isotope":[29],"incorporation,":[30],"but":[31],"recently,":[32],"comparative":[33],"label-free":[36,62],"LC(n)-MS":[37,63],"proteomics":[38],"data":[39,60],"has":[40],"emerged":[41],"as":[42],"alternative":[44],"approach.":[45],"In":[46],"this":[47],"chapter,":[48],"we":[49],"provide":[50],"overview":[52],"approaches":[56],"for":[57,68],"extracting":[58],"quantitative":[59,71],"from":[61],"experiments.":[64],"The":[65],"computational":[66],"procedure":[67],"recovering":[69],"information":[72],"is":[73],"outlined.":[74],"Examples":[75],"statistical":[77],"tests":[78],"used":[79],"evaluate":[81],"relevance":[83],"results":[85],"are":[86],"also":[87],"provided.":[88]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":2},{"year":2024,"cited_by_count":3},{"year":2023,"cited_by_count":5},{"year":2022,"cited_by_count":4},{"year":2021,"cited_by_count":7},{"year":2020,"cited_by_count":14},{"year":2019,"cited_by_count":12},{"year":2018,"cited_by_count":11},{"year":2017,"cited_by_count":6},{"year":2016,"cited_by_count":15},{"year":2015,"cited_by_count":8},{"year":2014,"cited_by_count":7},{"year":2013,"cited_by_count":8},{"year":2012,"cited_by_count":12}],"updated_date":"2026-04-15T08:11:43.952461","created_date":"2025-10-10T00:00:00"}