{"id":"https://openalex.org/W997635320","doi":null,"title":"Temperature variable and the efficiency of sperm mediated transfection of HPV16 DNA into cells.","display_name":"Temperature variable and the efficiency of sperm mediated transfection of HPV16 DNA into cells.","publication_year":2002,"publication_date":"2002-09-01","ids":{"openalex":"https://openalex.org/W997635320","mag":"997635320","pmid":"https://pubmed.ncbi.nlm.nih.gov/12364970"},"language":"en","primary_location":{"id":"pmid:12364970","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/12364970","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Asian journal of andrology","raw_type":"Journal Article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5049967949","display_name":"Ruslana Kadze","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1293502524","display_name":"Loma Linda University Medical Center","ror":"https://ror.org/03et1qs84","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1293502524"]},{"id":"https://openalex.org/I26347476","display_name":"Loma Linda University","ror":"https://ror.org/04bj28v14","country_code":"US","type":"education","lineage":["https://openalex.org/I26347476"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Ruslana Kadze","raw_affiliation_strings":["Center for Gertility and In Vitro Fertilization, Department of Gynecology and Obstetrics, and Department of Physiology and Pharmacology, Loma Linda University School of Medicine, Loma Linda, California 92350, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Center for Gertility and In Vitro Fertilization, Department of Gynecology and Obstetrics, and Department of Physiology and Pharmacology, Loma Linda University School of Medicine, Loma Linda, California 92350, USA","institution_ids":["https://openalex.org/I1293502524","https://openalex.org/I26347476"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5017052789","display_name":"Philip J. Chan","orcid":"https://orcid.org/0000-0003-2210-5084","observed_orcids":["https://orcid.org/0000-0003-2210-5084"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Philip J Chan","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5033714520","display_name":"John D. Jacobson","orcid":"https://orcid.org/0000-0003-1167-7909","observed_orcids":["https://orcid.org/0000-0003-1167-7909"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"John D Jacobson","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5043373993","display_name":"Johannah U Corselli","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Johannah U Corselli","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5103563017","display_name":"Alan King","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Alan King","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.2233,"has_fulltext":false,"cited_by_count":14,"citation_normalized_percentile":{"value":0.51643664,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":87,"max":93},"biblio":{"volume":"4","issue":"3","first_page":"169","last_page":"73"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10116","display_name":"Sperm and Testicular Function","score":0.7705000042915344,"subfield":{"id":"https://openalex.org/subfields/2743","display_name":"Reproductive Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10116","display_name":"Sperm and Testicular Function","score":0.7705000042915344,"subfield":{"id":"https://openalex.org/subfields/2743","display_name":"Reproductive Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T12929","display_name":"Animal Genetics and Reproduction","score":0.04349999874830246,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10364","display_name":"Reproductive Biology and Fertility","score":0.03590000048279762,"subfield":{"id":"https://openalex.org/subfields/2743","display_name":"Reproductive Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/sperm-mediated-gene-transfer","display_name":"sperm-mediated gene transfer","score":0.7900000214576721},{"id":"https://openalex.org/keywords/hpv-16","display_name":"HPV16","score":0.8730000257492065},{"id":"https://openalex.org/keywords/cumulus-cells","display_name":"cumulus cells","score":0.9200000166893005},{"id":"https://openalex.org/keywords/sperm-motility","display_name":"sperm motility","score":0.6840000152587891},{"id":"https://openalex.org/keywords/implantation-failure","display_name":"implantation failure","score":0.6639999747276306}],"concepts":[{"id":"https://openalex.org/C2781087480","wikidata":"https://www.wikidata.org/wiki/Q17145","display_name":"Sperm","level":2,"score":0.7987976670265198},{"id":"https://openalex.org/C16685009","wikidata":"https://www.wikidata.org/wiki/Q335057","display_name":"Andrology","level":1,"score":0.6898594498634338},{"id":"https://openalex.org/C54009773","wikidata":"https://www.wikidata.org/wiki/Q1429031","display_name":"Transfection","level":3,"score":0.5851699113845825},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.49964427947998047},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.4438186287879944},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3704718351364136},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.1913309395313263},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.13913103938102722},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.08733615279197693}],"mesh":[{"descriptor_ui":"D004279","descriptor_name":"DNA, Viral","qualifier_ui":"Q000493","qualifier_name":"pharmacokinetics","is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008297","descriptor_name":"Male","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013094","descriptor_name":"Spermatozoa","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true},{"descriptor_ui":"D013696","descriptor_name":"Temperature","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014162","descriptor_name":"Transfection","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D027383","descriptor_name":"Papillomaviridae","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true}],"locations_count":2,"locations":[{"id":"pmid:12364970","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/12364970","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Asian journal of andrology","raw_type":"Journal Article"},{"id":"mag:997635320","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/12364970","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.8048999905586243,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W3209885626","https://openalex.org/W4381620153","https://openalex.org/W7099678219","https://openalex.org/W2465439196","https://openalex.org/W2090830060","https://openalex.org/W1538683452","https://openalex.org/W2099642495","https://openalex.org/W2076236308","https://openalex.org/W2399545230","https://openalex.org/W2412361450"],"abstract_inverted_index":{"AIM:":[0],"To":[1],"pretreat":[2],"sperm":[3,24,49,88,154,210],"at":[4,54,78,97,141,176],"various":[5],"temperatures":[6],"before":[7],"exposure":[8],"to":[9,17,25,89,189,220,230],"human":[10],"papillomavirus":[11],"(HPV)":[12],"16":[13],"DNA":[14,47,94,151,187,195,219],"fragments":[15,188],"and":[16,38,111,201],"assess":[18],"the":[19,108,112,124,130,134,146,159,171,181,190,199,207,215,221],"efficiency":[20,86],"of":[21,87,114,122,149,184,209,217],"HPV":[22,46,93,150,186,194,218],"carrier":[23],"transfect":[26],"cumulus":[27,102,116,125,168,191,222],"cells.":[28,192],"METHODS:":[29],"Cumulus":[30],"cells":[31,69,117,126,223],"from":[32],"follicular":[33],"aspirates":[34],"were":[35,51,70,164],"obtained,":[36],"pooled":[37],"divided":[39],"into":[40],"culture":[41],"dishes":[42],"containing":[43],"Sybr":[44],"Gold-stained":[45],"carrying":[48],"that":[50],"either":[52],"pretreated":[53,140,175],"4":[55,142],"degree":[56,59,63,80,136,143,178],"C,":[57],"37":[58,79,135,177],"C":[60,64,81,137,144,179],"or":[61],"40":[62],"(n":[65],"=":[66],"5).":[67],"The":[68,85,193,204],"incubated":[71],"in":[72,75,133,167,198],"5%":[73],"CO(2)":[74],"air":[76],"mixture":[77],"for":[82,158,214],"24":[83],"hours.":[84],"take":[90],"up":[91],"fluorescent":[92,115,128,185],"was":[95,105,156,196],"determined":[96],"hour":[98],"0.":[99],"After":[100],"incubation,":[101],"cell":[103],"viability":[104,169],"assessed":[106],"using":[107],"eosin":[109],"method":[110],"percentages":[113],"determined.":[118],"RESULTS:":[119],"Over":[120],"half":[121],"all":[123],"became":[127],"with":[129],"highest":[131],"percentage":[132],"group.":[138],"Sperm":[139,174],"had":[145],"greatest":[147,182],"amount":[148,183],"fragments.":[152],"Total":[153],"motility":[155],"similar":[157],"3":[160],"pretreatment":[161],"groups.":[162,172],"There":[163],"no":[165],"differences":[166],"among":[170],"CONCLUSION:":[173],"transferred":[180],"observed":[197],"nuclear":[200],"cytoplasmic":[202],"compartments.":[203],"data":[205],"suggested":[206],"possibility":[208],"as":[211],"a":[212],"vector":[213],"transmission":[216],"surrounding":[224],"ovulated":[225],"oocytes,":[226],"which":[227],"might":[228],"lead":[229],"early":[231],"implantation":[232],"failures.":[233]},"counts_by_year":[{"year":2023,"cited_by_count":1},{"year":2021,"cited_by_count":1},{"year":2019,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}