{"id":"https://openalex.org/W997477034","doi":null,"title":"Preliminary characterization of the structural proteins of the coronaviruses, sialodacryoadenitis virus and Parker's rat coronavirus.","display_name":"Preliminary characterization of the structural proteins of the coronaviruses, sialodacryoadenitis virus and Parker's rat coronavirus.","publication_year":1994,"publication_date":"1994-04-01","ids":{"openalex":"https://openalex.org/W997477034","mag":"997477034","pmid":"https://pubmed.ncbi.nlm.nih.gov/8004548","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/1263673"},"language":"en","primary_location":{"id":"pmh:oai:europepmc.org:217536","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/1263673","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":null,"raw_type":"Text"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/1263673","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":null,"display_name":"M G Barker","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I79817857","display_name":"University of Guelph","ror":"https://ror.org/01r7awg59","country_code":"CA","type":"education","lineage":["https://openalex.org/I79817857"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"M G Barker","raw_affiliation_strings":["Department of Pathology, University of Guelph, Ontario"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pathology, University of Guelph, Ontario","institution_ids":["https://openalex.org/I79817857"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5110238506","display_name":"Dean H. Percy","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"D H Percy","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5055223386","display_name":"D J Hovland","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"D J Hovland","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5073519432","display_name":"Janet I. MacInnes","orcid":"https://orcid.org/0000-0002-1454-539X","observed_orcids":["https://orcid.org/0000-0002-1454-539X"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"J I MacInnes","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.7672,"has_fulltext":false,"cited_by_count":10,"citation_normalized_percentile":{"value":0.67396043,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":95},"biblio":{"volume":"58","issue":"2","first_page":"99","last_page":"103"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11495","display_name":"Animal Virus Infections Studies","score":0.9883000254631042,"subfield":{"id":"https://openalex.org/subfields/1103","display_name":"Animal Science and Zoology"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11495","display_name":"Animal Virus Infections Studies","score":0.9883000254631042,"subfield":{"id":"https://openalex.org/subfields/1103","display_name":"Animal Science and Zoology"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10118","display_name":"SARS-CoV-2 and COVID-19 Research","score":0.0038999998942017555,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11135","display_name":"Virology and Viral Diseases","score":0.0019000000320374966,"subfield":{"id":"https://openalex.org/subfields/2713","display_name":"Epidemiology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/sialodacryoadenitis-virus","display_name":"sialodacryoadenitis virus","score":0.9850000143051147},{"id":"https://openalex.org/keywords/coronavirus-structural-proteins","display_name":"coronavirus structural proteins","score":0.8130000233650208},{"id":"https://openalex.org/keywords/matrix-protein","display_name":"matrix protein","score":0.6100000143051147},{"id":"https://openalex.org/keywords/nucleocapsid-protein","display_name":"nucleocapsid protein","score":0.9980000257492065},{"id":"https://openalex.org/keywords/spike-protein","display_name":"spike protein","score":0.9480000138282776},{"id":"https://openalex.org/keywords/monoclonal-antibodies","display_name":"monoclonal antibodies","score":0.7789999842643738},{"id":"https://openalex.org/keywords/antigenic-cross-reactivity","display_name":"antigenic cross-reactivity","score":0.6740000247955322}],"concepts":[{"id":"https://openalex.org/C2777648638","wikidata":"https://www.wikidata.org/wiki/Q57751738","display_name":"Coronavirus","level":5,"score":0.8292326927185059},{"id":"https://openalex.org/C2779567548","wikidata":"https://www.wikidata.org/wiki/Q18907882","display_name":"Mouse hepatitis virus","level":5,"score":0.8180747628211975},{"id":"https://openalex.org/C2776844884","wikidata":"https://www.wikidata.org/wiki/Q1134583","display_name":"Coronaviridae","level":5,"score":0.7626429796218872},{"id":"https://openalex.org/C956191","wikidata":"https://www.wikidata.org/wiki/Q930144","display_name":"Polyclonal antibodies","level":3,"score":0.76008141040802},{"id":"https://openalex.org/C91523082","wikidata":"https://www.wikidata.org/wiki/Q420496","display_name":"Antiserum","level":3,"score":0.7323999404907227},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.6740121245384216},{"id":"https://openalex.org/C542903549","wikidata":"https://www.wikidata.org/wiki/Q422248","display_name":"Monoclonal antibody","level":3,"score":0.6438643336296082},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5559657216072083},{"id":"https://openalex.org/C2522874641","wikidata":"https://www.wikidata.org/wiki/Q808","display_name":"Virus","level":2,"score":0.5393296480178833},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.475286602973938},{"id":"https://openalex.org/C2776810377","wikidata":"https://www.wikidata.org/wiki/Q4950554","display_name":"Bovine coronavirus","level":5,"score":0.46955233812332153},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.4456327259540558},{"id":"https://openalex.org/C7623868","wikidata":"https://www.wikidata.org/wiki/Q1967135","display_name":"Centrifugation","level":2,"score":0.43869972229003906},{"id":"https://openalex.org/C3019983190","wikidata":"https://www.wikidata.org/wiki/Q157661","display_name":"Hepatitis a virus","level":3,"score":0.4242202639579773},{"id":"https://openalex.org/C3008058167","wikidata":"https://www.wikidata.org/wiki/Q84263196","display_name":"Coronavirus disease 2019 (COVID-19)","level":4,"score":0.2333415448665619},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.13990074396133423},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0906711220741272},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.0752946138381958},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.0},{"id":"https://openalex.org/C2779134260","wikidata":"https://www.wikidata.org/wiki/Q12136","display_name":"Disease","level":2,"score":0.0},{"id":"https://openalex.org/C524204448","wikidata":"https://www.wikidata.org/wiki/Q788926","display_name":"Infectious disease (medical specialty)","level":3,"score":0.0}],"mesh":[{"descriptor_ui":"D000645","descriptor_name":"Ammonium Sulfate","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000911","descriptor_name":"Antibodies, Monoclonal","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D002460","descriptor_name":"Cell Line","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002499","descriptor_name":"Centrifugation, Density Gradient","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007106","descriptor_name":"Immune Sera","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D008970","descriptor_name":"Molecular Weight","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011232","descriptor_name":"Chemical Precipitation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015151","descriptor_name":"Immunoblotting","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015678","descriptor_name":"Viral Structural Proteins","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D015678","descriptor_name":"Viral Structural Proteins","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D015678","descriptor_name":"Viral Structural Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D017941","descriptor_name":"Coronavirus, Rat","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D051381","descriptor_name":"Rats","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":4,"locations":[{"id":"pmh:oai:europepmc.org:217536","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/1263673","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":null,"raw_type":"Text"},{"id":"pmh:oai:pubmedcentral.nih.gov:1263673","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/1263673","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Can J Vet Res","raw_type":"Text"},{"id":"pmid:8004548","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/8004548","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Canadian journal of veterinary research = Revue canadienne de recherche veterinaire","raw_type":"Journal Article"},{"id":"mag:997477034","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/8004548","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":{"id":"pmh:oai:europepmc.org:217536","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/1263673","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":null,"raw_type":"Text"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":18,"referenced_works":["https://openalex.org/W39118494","https://openalex.org/W1856288802","https://openalex.org/W1971005624","https://openalex.org/W1982393262","https://openalex.org/W1984002543","https://openalex.org/W2013930155","https://openalex.org/W2014641553","https://openalex.org/W2018289835","https://openalex.org/W2063107361","https://openalex.org/W2078027579","https://openalex.org/W2084864009","https://openalex.org/W2097346213","https://openalex.org/W2100837269","https://openalex.org/W2124218787","https://openalex.org/W2139090502","https://openalex.org/W2413342754","https://openalex.org/W2422228764","https://openalex.org/W2464572918"],"related_works":["https://openalex.org/W1995602810","https://openalex.org/W169081299","https://openalex.org/W2001745911","https://openalex.org/W2153667801","https://openalex.org/W2013930155","https://openalex.org/W1985105640","https://openalex.org/W1723090750","https://openalex.org/W193167121","https://openalex.org/W156322735","https://openalex.org/W2073076942"],"abstract_inverted_index":{"A":[0],"procedure":[1],"was":[2,136],"developed":[3],"for":[4,91,114],"the":[5,9,58,70,98,125,133,142,146],"partial":[6],"purification":[7],"of":[8,57,61,73,84,105,145,157],"rat":[10,17,147],"coronaviruses,":[11,148],"sialodacryoadenitis":[12],"virus":[13,64],"(SDAV)":[14],"and":[15,22,35,46,53,86,109,127,150],"Parker's":[16],"coronavirus":[18],"(PRC).":[19],"The":[20,43],"SDAV":[21,45,85,108,149],"PRC":[23,47,87,110,151],"were":[24,49],"replicated":[25],"in":[26,82,107],"L-2":[27],"cell":[28],"monolayer":[29],"cultures,":[30],"precipitated":[31],"with":[32,55,103,124],"ammonium":[33],"sulphate,":[34],"further":[36],"concentrated":[37],"using":[38],"sucrose":[39],"density":[40],"gradient":[41],"centrifugation.":[42],"major":[44],"proteins":[48,76,104,135,144],"identified":[50],"by":[51],"immunoblotting":[52],"compared":[54],"those":[56,156],"JHM":[59,74,99],"strain":[60],"mouse":[62],"hepatitis":[63],"(MHV-JHM).":[65],"Monoclonal":[66],"antibodies":[67],"(MAb)":[68],"against":[69,97],"M":[71,126],"protein":[72,101],"recognized":[75],"interpreted":[77],"to":[78,118,155],"be":[79],"slightly":[80],"smaller":[81],"immunoblots":[83],"(22.8":[88],"vs":[89],"23K":[90],"JHM).":[92,115],"Similarly,":[93],"a":[94],"monoclonal":[95],"antibody":[96],"N":[100,128],"reacted":[102],"53K":[106],"(vs":[111],"56":[112],"K":[113],"Polyclonal":[116],"antisera":[117],"all":[119],"three":[120],"viruses":[121],"also":[122],"cross-reacted":[123],"proteins.":[129],"Some":[130],"cross-reactivity":[131],"amongst":[132],"S":[134],"observed.":[137],"Based":[138],"on":[139],"these":[140],"data,":[141],"structural":[143],"are":[152],"closely":[153],"related":[154],"MHV-JHM.":[158]},"counts_by_year":[{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":2},{"year":2019,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}