{"id":"https://openalex.org/W996958699","doi":null,"title":"\u7248\u7eb3\u5fae\u578b\u732a\u8fd1\u4ea4\u7cfbTNF-\u03b1\u57fa\u56e0\u514b\u9686\u3001\u5e8f\u5217\u5206\u6790\u53ca\u5206\u5b50\u7cfb\u7edf\u8fdb\u5316\u7814\u7a76","display_name":"\u7248\u7eb3\u5fae\u578b\u732a\u8fd1\u4ea4\u7cfbTNF-\u03b1\u57fa\u56e0\u514b\u9686\u3001\u5e8f\u5217\u5206\u6790\u53ca\u5206\u5b50\u7cfb\u7edf\u8fdb\u5316\u7814\u7a76","publication_year":2012,"publication_date":"2012-01-01","ids":{"openalex":"https://openalex.org/W996958699","mag":"996958699"},"language":"zh","primary_location":{"id":"mag:996958699","doi":null,"is_oa":false,"landing_page_url":"http://www.cqvip.com/QK/94396X/201206/44491794.html","pdf_url":null,"source":{"id":"https://openalex.org/S2764395222","display_name":"Xi'nan nongye xuebao","issn_l":"1001-4829","issn":["1001-4829"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4322698808","host_organization_name":"Editorial Department of Southwest China Journal of Agricultural Sciences","host_organization_lineage":["https://openalex.org/P4322698808"],"host_organization_lineage_names":["Editorial Department of Southwest China Journal of Agricultural Sciences"],"type":"journal"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"\u897f\u5357\u519c\u4e1a\u5b66\u62a5","raw_type":null},"volume":null,"type":"article","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5031389959","display_name":"\u5218\u4e3d\u4ed9","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"\u5218\u4e3d\u4ed9","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5069662664","display_name":"\u738b\u9510","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"\u738b\u9510","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5121912135","display_name":"Hailong Huo","orcid":"https://orcid.org/0000-0001-5047-7307","observed_orcids":["https://orcid.org/0000-0001-5047-7307"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"\u970d\u6d77\u9f99","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5008514783","display_name":"\u970d\u91d1\u9f99","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"\u970d\u91d1\u9f99","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5091300835","display_name":"\u8d75\u8dc3","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"\u8d75\u8dc3","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5088217769","display_name":"\u6731\u56fd\u742a","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"\u6731\u56fd\u742a","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5049695384","display_name":"\u66fe\u517b\u5fd7","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"\u66fe\u517b\u5fd7","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.0,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"25","issue":"6","first_page":"2327","last_page":"2332"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10371","display_name":"Immune Response and Inflammation","score":0.2870999872684479,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10371","display_name":"Immune Response and Inflammation","score":0.2870999872684479,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12322","display_name":"Animal health and immunology","score":0.22579999268054962,"subfield":{"id":"https://openalex.org/subfields/1103","display_name":"Animal Science and Zoology"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10594","display_name":"Genetic and phenotypic traits in livestock","score":0.09139999747276306,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/tnf-alpha","display_name":"TNF-\u03b1","score":0.8899999856948853},{"id":"https://openalex.org/keywords/inbred-lines","display_name":"inbred lines","score":0.7549999952316284},{"id":"https://openalex.org/keywords/molecular-phylogenetic-analysis","display_name":"molecular phylogenetic analysis","score":0.7239999771118164},{"id":"https://openalex.org/keywords/tissue-gene-expression","display_name":"gene expression in tissues","score":0.7720000147819519},{"id":"https://openalex.org/keywords/sequence-homology","display_name":"sequence homology","score":0.6499999761581421},{"id":"https://openalex.org/keywords/amino-acid-sequence","display_name":"amino acid sequence","score":0.4729999899864197},{"id":"https://openalex.org/keywords/comparative-sequence-analysis","display_name":"sequence comparison","score":0.3630000054836273}],"concepts":[{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.29061800241470337}],"mesh":[],"locations_count":1,"locations":[{"id":"mag:996958699","doi":null,"is_oa":false,"landing_page_url":"http://www.cqvip.com/QK/94396X/201206/44491794.html","pdf_url":null,"source":{"id":"https://openalex.org/S2764395222","display_name":"Xi'nan nongye xuebao","issn_l":"1001-4829","issn":["1001-4829"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4322698808","host_organization_name":"Editorial Department of Southwest China Journal of Agricultural Sciences","host_organization_lineage":["https://openalex.org/P4322698808"],"host_organization_lineage_names":["Editorial Department of Southwest China Journal of Agricultural Sciences"],"type":"journal"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"\u897f\u5357\u519c\u4e1a\u5b66\u62a5","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W22245098","https://openalex.org/W2741510576","https://openalex.org/W2733406339","https://openalex.org/W2270429742","https://openalex.org/W964995747","https://openalex.org/W2030426916","https://openalex.org/W1587842720","https://openalex.org/W2341354466","https://openalex.org/W2049168087","https://openalex.org/W3102034976"],"abstract_inverted_index":{"\u5206\u522b\u63d0\u53d6\u7248\u7eb3\u5fae\u578b\u732a\u8fd1\u4ea4\u7cfb18\u79cd\u7ec4\u7ec7\u7684RNA\uff0c\u53cd\u8f6c\u5f55\u4e3acDNA\uff0c\u5229\u7528RT-PCR\u65b9\u6cd5\u6269\u589e\u80bf\u7624\u574f\u6b7b\u56e0\u5b50\uff08TNF-\u03b1\uff09\u57fa\u56e0\u7f16\u7801\u533a\u5168\u957f\u5e8f\u5217\uff0c\u5c06\u7eaf\u5316\u7684\u7247\u6bb5\u4e0epMDl8-T\u8f7d\u4f53\u8fde\u63a5\uff0c\u8f6c\u5316\u5927\u80a0\u6746\u83ccDH5eL\uff0c\u7b5b\u9009\u9633\u6027\u514b\u9686\u5e76\u6d4b\u5b9a\u5176\u5e8f\u5217\uff0c\u5229\u7528\u751f\u7269\u4fe1\u606f\u5b66\u65b9\u6cd5\u5206\u6790\u5176\u5e8f\u5217\u7279\u5f81\uff0c\u5e76\u4e0e\u5176\u4ed615\u4e2a\u7269\u79cd\u6784\u5efa\u7cfb\u7edf\u8fdb\u5316\u6811\u3002\u540c\u65f6\u91c7\u7528\u534a\u5b9a\u91cfRT-PCR\u65b9\u6cd5\u5206\u6790TNF-\u03b1\u57fa\u56e0\u5728\u7248\u7eb3\u5fae\u578b\u732a\u8fd1\u4ea4\u7cfb18\u79cd\u7ec4\u7ec7\u4e2d\u7684\u8868\u8fbe\u60c5\u51b5\u3002\u7ed3\u679c\u663e\u793a\uff0c\u7248\u7eb3\u5fae\u578b\u732a\u8fd1\u4ea4\u7cfbTNF-\u03b1\u57fa\u56e0\u7f16\u7801\u533a\u5168\u957f699bp\uff08GenBank\u767b\u5f55\u53f7\uff1aJF831365\uff09\uff0c\u7f16\u7801232\u4e2a\u6c28\u57fa\u9178\u3002\u6c28\u57fa\u9178\u5e8f\u5217\u540c\u6e90\u6027\u5206\u6790\u8868\u660e\uff0c\u4e0e\u732b\u7b4912\u4e2a\u7269\u79cd\u5177\u670980\uff05\u4ee5\u4e0a\u7684\u76f8\u4f3c\u6027\uff0c\u5206\u5b50\u7cfb\u7edf\u8fdb\u5316\u8868\u660e\u4e0e\u725b\u3001\u5c71\u7f8a\u3001\u7ef5\u7f8a\u53ca\u6d77\u8c5a\u7684\u5173\u7cfb\u8f83\u8fd1\u3002\u591a\u7ec4\u7ec7\u534a\u5b9a\u91cfRT-PCR\u7814\u7a76\u8868\u660e\uff0cTNF-\u03b1mRNA\u5728\u7248\u7eb3\u5fae\u578b\u732a\u8fd1\u4ea4\u7cfb\u7684\u591a\u4e2a\u7ec4\u7ec7\u4e2d\u5747\u6709\u8868\u8fbe\uff0c\u5176\u4e2d\u5728\u4e2d\u8111\u3001\u5375\u5de2\u3001\u813e\u3001\u80ba\u3001\u795e\u7ecf\u53ca\u80c3\u4e2d\u8868\u8fbe\u91cf\u8f83\u9ad8\u3002":[0]},"counts_by_year":[],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}