{"id":"https://openalex.org/W985186412","doi":null,"title":"[Functional cell surface display of endo-beta-1, 3-1, 4-glucanase in Lactococcus lactis using N-acetylmuraminidase as the anchoring motif].","display_name":"[Functional cell surface display of endo-beta-1, 3-1, 4-glucanase in Lactococcus lactis using N-acetylmuraminidase as the anchoring motif].","publication_year":2009,"publication_date":"2009-01-01","ids":{"openalex":"https://openalex.org/W985186412","mag":"985186412","pmid":"https://pubmed.ncbi.nlm.nih.gov/19441232"},"language":"zh","primary_location":{"id":"pmid:19441232","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/19441232","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","raw_type":"Journal Article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5062741073","display_name":"\u674e\u6653\u534e","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I204823248","display_name":"Huazhong Agricultural University","ror":"https://ror.org/023b72294","country_code":"CN","type":"education","lineage":["https://openalex.org/I204823248"]},{"id":"https://openalex.org/I4391768000","display_name":"State Key Laboratory of Agricultural Microbiology","ror":"https://ror.org/003hwx626","country_code":"CN","type":"facility","lineage":["https://openalex.org/I204823248","https://openalex.org/I4391768000"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Xiaohua Li","raw_affiliation_strings":["State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China","institution_ids":["https://openalex.org/I204823248","https://openalex.org/I4391768000"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5056711945","display_name":"\u9ec4\u65b0\u51e4","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Xinfeng Huang","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5056711945","display_name":"\u9ec4\u65b0\u51e4","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Xiaohu Shao","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5049363926","display_name":"\u90b5\u5c0f\u864e","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Lin Li","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.4023,"has_fulltext":false,"cited_by_count":3,"citation_normalized_percentile":{"value":0.68209802,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":93},"biblio":{"volume":"25","issue":"1","first_page":"89","last_page":"94"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10141","display_name":"Probiotics and Fermented Foods","score":0.4377000033855438,"subfield":{"id":"https://openalex.org/subfields/1106","display_name":"Food Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10141","display_name":"Probiotics and Fermented Foods","score":0.4377000033855438,"subfield":{"id":"https://openalex.org/subfields/1106","display_name":"Food Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.38429999351501465,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.02850000001490116,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/lactococcus-lactis","display_name":"Lactococcus lactis","score":0.9430000185966492},{"id":"https://openalex.org/keywords/acma","display_name":"ACMA","score":0.7360000014305115},{"id":"https://openalex.org/keywords/surface-display","display_name":"surface display","score":0.8769999742507935},{"id":"https://openalex.org/keywords/beta-1-3-1-4-glucanase","display_name":"\u03b2-1,3-1,4-glucanase","score":0.9330000281333923},{"id":"https://openalex.org/keywords/heterologous-expression","display_name":"heterologous expression","score":0.6150000095367432},{"id":"https://openalex.org/keywords/cell-surface-display","display_name":"cell surface display","score":0.4390000104904175}],"concepts":[{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.39663153886795044}],"mesh":[{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D006026","descriptor_name":"Glycoside Hydrolases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D006026","descriptor_name":"Glycoside Hydrolases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011993","descriptor_name":"Recombinant Fusion Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D011993","descriptor_name":"Recombinant Fusion Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011995","descriptor_name":"Recombination, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013294","descriptor_name":"Lactococcus lactis","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D013294","descriptor_name":"Lactococcus lactis","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D018274","descriptor_name":"Electroporation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D044644","descriptor_name":"Endo-1,3(4)-beta-Glucanase","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D044644","descriptor_name":"Endo-1,3(4)-beta-Glucanase","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D049452","descriptor_name":"Green Fluorescent Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D049452","descriptor_name":"Green Fluorescent Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false}],"locations_count":3,"locations":[{"id":"pmid:19441232","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/19441232","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","raw_type":"Journal Article"},{"id":"mag:2412442392","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/19441232","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null},{"id":"mag:985186412","doi":null,"is_oa":false,"landing_page_url":"http://cqvip.com/QK/92387X/200901/29434685.html","pdf_url":null,"source":{"id":"https://openalex.org/S4306555132","display_name":"\u751f\u7269\u5de5\u7a0b\u5b66\u62a5","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"\u751f\u7269\u5de5\u7a0b\u5b66\u62a5","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":19,"referenced_works":["https://openalex.org/W1832138672","https://openalex.org/W1968873177","https://openalex.org/W1979547207","https://openalex.org/W1985244131","https://openalex.org/W2001745732","https://openalex.org/W2021741281","https://openalex.org/W2050466522","https://openalex.org/W2070082076","https://openalex.org/W2079588178","https://openalex.org/W2125621374","https://openalex.org/W2135685789","https://openalex.org/W2140544780","https://openalex.org/W2151277682","https://openalex.org/W2160266626","https://openalex.org/W2164387662","https://openalex.org/W2167525455","https://openalex.org/W2340120045","https://openalex.org/W2406218677","https://openalex.org/W2804007336"],"related_works":["https://openalex.org/W563991171","https://openalex.org/W3166620800","https://openalex.org/W91199208","https://openalex.org/W2213715989","https://openalex.org/W2758630938","https://openalex.org/W2582902390","https://openalex.org/W226175775","https://openalex.org/W564350136","https://openalex.org/W7165724969","https://openalex.org/W215066417"],"abstract_inverted_index":{"In":[0],"this":[1],"report,":[2],"we":[3],"utilized":[4],"N-Acetylmuraminidase":[5],"(AcmA)":[6],"to":[7,167,180],"develop":[8],"a":[9,119,158,176],"whole-cell":[10,160],"catalyst":[11],"of":[12,63,81,108,123,189],"endo-beta-1,":[13,125],"3-1,":[14,126],"4-glucanase":[15,127],"in":[16,67,135],"Lactococcus":[17],"lactis.":[18,191],"The":[19],"PCR-amplified":[20,120],"full-length":[21],"acmA":[22,115],"gene":[23,34,128],"from":[24,130],"L.":[25,69,144,149,190],"lactis":[26,46,70,145,150],"MB191":[27],"was":[28,98,116],"fused":[29,117],"with":[30,77,118],"the":[31,39,43,52,60,68,78,101,109,124,136,147,153,168,182,186],"green":[32],"fluorescent":[33],"(gfp),":[35],"followed":[36],"by":[37],"ligating":[38],"chimeric":[40],"acmA-gfp":[41],"into":[42,143],"Escherichia":[44],"coli-L.":[45],"shuttle":[47],"expression":[48,62],"vector":[49],"pMG36k,":[50],"yielding":[51],"recombinant":[53,137],"plasmid":[54,138],"pMB137.":[55],"SDS-PAGE":[56],"analysis":[57,94],"showed":[58],"that":[59,96],"constitutive":[61],"AcmA-GFP":[64,97],"fusion":[65,112,155],"protein":[66],"AS1.2829":[71],"construct":[72],"harboring":[73],"pMB137":[74],"(named":[75],"MB137),":[76],"predicted":[79],"Mr":[80],"74":[82],"kD.":[83],"Western":[84],"blotting,":[85],"GFP":[86],"specific":[87],"fluorescence":[88],"intensity":[89],"assays":[90],"and":[91],"flow":[92],"cytometry":[93],"confirmed":[95],"immobilized":[99],"on":[100,185],"outer":[102],"membrane,":[103],"which":[104],"constituted":[105],"approx.":[106],"35%":[107],"total":[110],"intracellular":[111],"protein.":[113],"Furthermore,":[114],"encoding":[121],"fragment":[122],"(gls)":[129],"Bacillus":[131],"sublitis":[132],"BF7658,":[133],"resulting":[134],"pMB138.":[139],"By":[140],"transferring":[141],"pMB138":[142],"AS1.2829,":[146],"derived":[148],"MB138":[151],"expressing":[152],"AcmA-GLS":[154],"enzyme":[156,184],"exhibited":[157],"distinct":[159],"glucanase":[161],"activity":[162],"(by":[163],"12":[164],"U/mL)":[165],"compared":[166],"control":[169],"strain,":[170],"indicating":[171],"AcmA":[172],"had":[173],"served":[174],"as":[175],"functional":[177],"anchoring":[178],"motif":[179],"immobilize":[181],"heterologous":[183],"cell":[187],"surface":[188]},"counts_by_year":[{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}