{"id":"https://openalex.org/W970953018","doi":"https://doi.org/10.2460/ajvr.1996.57.08.1157","title":"Development of monoclonal antibodies for measurement of immunoglobulin G antibody responses in Blue and Gold Macaws (Ara ararauna)","display_name":"Development of monoclonal antibodies for measurement of immunoglobulin G antibody responses in Blue and Gold Macaws (Ara ararauna)","publication_year":1996,"publication_date":"1996-08-01","ids":{"openalex":"https://openalex.org/W970953018","doi":"https://doi.org/10.2460/ajvr.1996.57.08.1157","mag":"970953018","pmid":"https://pubmed.ncbi.nlm.nih.gov/8836367"},"language":"en","primary_location":{"id":"doi:10.2460/ajvr.1996.57.08.1157","doi":"https://doi.org/10.2460/ajvr.1996.57.08.1157","is_oa":true,"landing_page_url":"https://doi.org/10.2460/ajvr.1996.57.08.1157","pdf_url":"https://avmajournals.avma.org/downloadpdf/view/journals/ajvr/57/8/ajvr.1996.57.08.1157.pdf","source":{"id":"https://openalex.org/S196975874","display_name":"American Journal of Veterinary Research","issn_l":"0002-9645","issn":["0002-9645","1943-5681"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-d","jufo-2","medline","norway-1","oarl","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4322545313","host_organization_name":"American Veterinary Medical Association","host_organization_lineage":["https://openalex.org/P4322545313"],"host_organization_lineage_names":["American Veterinary Medical Association"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"American Journal of Veterinary Research","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://avmajournals.avma.org/downloadpdf/view/journals/ajvr/57/8/ajvr.1996.57.08.1157.pdf","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5113701548","display_name":"Nancy P. Lung","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I33213144","display_name":"University of Florida","ror":"https://ror.org/02y3ad647","country_code":"US","type":"education","lineage":["https://openalex.org/I33213144"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Nancy P. Lung","raw_affiliation_strings":["From the Department of Small Animal Clinical Sciences, College of Veterinary Medicine (Lung, Thompson, Kollias Jr), and Department of Pathology, College of Medicine (Klein), University of Florida, Gainesville, FL 32610"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From the Department of Small Animal Clinical Sciences, College of Veterinary Medicine (Lung, Thompson, Kollias Jr), and Department of Pathology, College of Medicine (Klein), University of Florida, Gainesville, FL 32610","institution_ids":["https://openalex.org/I33213144"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5084438850","display_name":"James P. Thompson","orcid":"https://orcid.org/0000-0002-4292-6747","observed_orcids":["https://orcid.org/0000-0002-4292-6747"]},"institutions":[{"id":"https://openalex.org/I33213144","display_name":"University of Florida","ror":"https://ror.org/02y3ad647","country_code":"US","type":"education","lineage":["https://openalex.org/I33213144"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"James P. Thompson","raw_affiliation_strings":["From the Department of Small Animal Clinical Sciences, College of Veterinary Medicine (Lung, Thompson, Kollias Jr), and Department of Pathology, College of Medicine (Klein), University of Florida, Gainesville, FL 32610"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From the Department of Small Animal Clinical Sciences, College of Veterinary Medicine (Lung, Thompson, Kollias Jr), and Department of Pathology, College of Medicine (Klein), University of Florida, Gainesville, FL 32610","institution_ids":["https://openalex.org/I33213144"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5027592848","display_name":"George V. Kollias","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I33213144","display_name":"University of Florida","ror":"https://ror.org/02y3ad647","country_code":"US","type":"education","lineage":["https://openalex.org/I33213144"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"George V. Kollias","raw_affiliation_strings":["From the Department of Small Animal Clinical Sciences, College of Veterinary Medicine (Lung, Thompson, Kollias Jr), and Department of Pathology, College of Medicine (Klein), University of Florida, Gainesville, FL 32610"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From the Department of Small Animal Clinical Sciences, College of Veterinary Medicine (Lung, Thompson, Kollias Jr), and Department of Pathology, College of Medicine (Klein), University of Florida, Gainesville, FL 32610","institution_ids":["https://openalex.org/I33213144"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5043466976","display_name":"Paul Klein","orcid":"https://orcid.org/0000-0002-0141-3902","observed_orcids":["https://orcid.org/0000-0002-0141-3902"]},"institutions":[{"id":"https://openalex.org/I33213144","display_name":"University of Florida","ror":"https://ror.org/02y3ad647","country_code":"US","type":"education","lineage":["https://openalex.org/I33213144"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Paul A. Klein","raw_affiliation_strings":["From the Department of Small Animal Clinical Sciences, College of Veterinary Medicine (Lung, Thompson, Kollias Jr), and Department of Pathology, College of Medicine (Klein), University of Florida, Gainesville, FL 32610"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From the Department of Small Animal Clinical Sciences, College of Veterinary Medicine (Lung, Thompson, Kollias Jr), and Department of Pathology, College of Medicine (Klein), University of Florida, Gainesville, FL 32610","institution_ids":["https://openalex.org/I33213144"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I33213144"],"apc_list":null,"apc_paid":null,"fwci":0.2544,"has_fulltext":true,"cited_by_count":22,"citation_normalized_percentile":{"value":0.49030052,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":93},"biblio":{"volume":"57","issue":"8","first_page":"1157","last_page":"1161"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11016","display_name":"Monoclonal and Polyclonal Antibodies Research","score":0.7333999872207642,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11016","display_name":"Monoclonal and Polyclonal Antibodies Research","score":0.7333999872207642,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12322","display_name":"Animal health and immunology","score":0.20479999482631683,"subfield":{"id":"https://openalex.org/subfields/1103","display_name":"Animal Science and Zoology"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11495","display_name":"Animal Virus Infections Studies","score":0.014299999922513962,"subfield":{"id":"https://openalex.org/subfields/1103","display_name":"Animal Science and Zoology"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/monoclonal-antibodies","display_name":"monoclonal antibodies","score":0.9909999966621399},{"id":"https://openalex.org/keywords/igg","display_name":"IgG","score":0.8820000290870667},{"id":"https://openalex.org/keywords/blue-and-yellow-macaw","display_name":"blue-and-yellow macaw","score":0.9919999837875366},{"id":"https://openalex.org/keywords/hybridoma-cells","display_name":"hybridoma","score":0.6909999847412109},{"id":"https://openalex.org/keywords/bovine-serum-albumin","display_name":"bovine serum albumin","score":0.7839999794960022},{"id":"https://openalex.org/keywords/humoral-immune-response","display_name":"humoral immune response","score":0.9150000214576721},{"id":"https://openalex.org/keywords/antibody-titers","display_name":"antibody titers","score":0.6399999856948853},{"id":"https://openalex.org/keywords/secondary-antibody-response","display_name":"secondary antibody response","score":0.7120000123977661}],"concepts":[{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.7781437635421753},{"id":"https://openalex.org/C542903549","wikidata":"https://www.wikidata.org/wiki/Q422248","display_name":"Monoclonal antibody","level":3,"score":0.6258437633514404},{"id":"https://openalex.org/C2780898057","wikidata":"https://www.wikidata.org/wiki/Q79448","display_name":"Immunoglobulin G","level":3,"score":0.42335814237594604},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3929092586040497},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.3651942014694214},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.3401709198951721},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.33496007323265076},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.32404446601867676}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000911","descriptor_name":"Antibodies, Monoclonal","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D000917","descriptor_name":"Antibody Formation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000918","descriptor_name":"Antibody Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001717","descriptor_name":"Birds","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":true},{"descriptor_ui":"D002417","descriptor_name":"Cattle","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003429","descriptor_name":"Cross Reactions","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004797","descriptor_name":"Enzyme-Linked Immunosorbent Assay","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D005260","descriptor_name":"Female","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007074","descriptor_name":"Immunoglobulin G","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D007074","descriptor_name":"Immunoglobulin G","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":true},{"descriptor_ui":"D007143","descriptor_name":"Immunoglobulin Heavy Chains","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D007143","descriptor_name":"Immunoglobulin Heavy Chains","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":true},{"descriptor_ui":"D008297","descriptor_name":"Male","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012680","descriptor_name":"Sensitivity and Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012710","descriptor_name":"Serum Albumin, Bovine","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":true},{"descriptor_ui":"D013045","descriptor_name":"Species Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015153","descriptor_name":"Blotting, Western","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.2460/ajvr.1996.57.08.1157","doi":"https://doi.org/10.2460/ajvr.1996.57.08.1157","is_oa":true,"landing_page_url":"https://doi.org/10.2460/ajvr.1996.57.08.1157","pdf_url":"https://avmajournals.avma.org/downloadpdf/view/journals/ajvr/57/8/ajvr.1996.57.08.1157.pdf","source":{"id":"https://openalex.org/S196975874","display_name":"American Journal of Veterinary Research","issn_l":"0002-9645","issn":["0002-9645","1943-5681"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-d","jufo-2","medline","norway-1","oarl","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4322545313","host_organization_name":"American Veterinary Medical Association","host_organization_lineage":["https://openalex.org/P4322545313"],"host_organization_lineage_names":["American Veterinary Medical Association"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"American Journal of Veterinary Research","raw_type":"journal-article"},{"id":"pmid:8836367","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/8836367","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"American journal of veterinary research","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.2460/ajvr.1996.57.08.1157","doi":"https://doi.org/10.2460/ajvr.1996.57.08.1157","is_oa":true,"landing_page_url":"https://doi.org/10.2460/ajvr.1996.57.08.1157","pdf_url":"https://avmajournals.avma.org/downloadpdf/view/journals/ajvr/57/8/ajvr.1996.57.08.1157.pdf","source":{"id":"https://openalex.org/S196975874","display_name":"American Journal of Veterinary Research","issn_l":"0002-9645","issn":["0002-9645","1943-5681"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-d","jufo-2","medline","norway-1","oarl","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4322545313","host_organization_name":"American Veterinary Medical Association","host_organization_lineage":["https://openalex.org/P4322545313"],"host_organization_lineage_names":["American Veterinary Medical Association"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"American Journal of Veterinary Research","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W970953018.pdf","grobid_xml":"https://content.openalex.org/works/W970953018.grobid-xml"},"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2293081924","https://openalex.org/W2802048368","https://openalex.org/W2187196971","https://openalex.org/W2987225386","https://openalex.org/W2366180913","https://openalex.org/W3149080020","https://openalex.org/W2063326992","https://openalex.org/W2367262527","https://openalex.org/W2900732951","https://openalex.org/W1971026746"],"abstract_inverted_index":{"OBJECTIVES:":[0],"To":[1],"produce":[2],"monoclonal":[3],"antibodies":[4],"(MAB)":[5],"with":[6,29,78,94,119,174,194,247],"specificity":[7,280],"for":[8,108,133,220,281],"the":[9,37,155,230,275,290,298],"heavy":[10],"chain":[11],"of":[12,26,39,70,81,97,110,135,171,197,202,213,234,250,255,267,292,300],"macaw":[13,140,221],"IgG;":[14],"to":[15,22,35,68,228,278],"incorporate":[16],"these":[17],"MAB":[18,160,218],"into":[19,164],"an":[20,165,211,265],"ELISA":[21,145,166,227],"measure":[23],"IgG":[24,124,134,169],"responses":[25,170,209,233,262,295],"macaws":[27,172],"inoculated":[28,77,93,173,246],"bovine":[30],"serum":[31],"albumin":[32],"(BSA);":[33],"and":[34,43,50,55,61,88,102,129,138,146,154,179,185,238,296],"evaluate":[36,229],"antigenicity":[38],"BSA":[40,82,98],"in":[41,206,226,259,289,297],"Blue":[42,49,178,237],"Gold":[44,51,180,239],"Macaws.":[45],"ANIMALS:":[46],"Four":[47],"1-year-old":[48,236],"Macaws,":[52],"2":[53,56,71],"males":[54],"females.":[57],"PROCEDURE:":[58],"1":[59,62,64],"male":[60],"female":[63],"were":[65,76,92,114],"randomly":[66],"assigned":[67],"each":[69],"study":[72],"groups.":[73],"Group-1":[74],"birds":[75,91],"200":[79,95,195,248],"micrograms":[80,96,196,249],"on":[83,99],"days":[84,100,191,204,257],"0,":[85],"14,":[86],"28,":[87],"42.":[89],"Group-2":[90],"0":[101],"28.":[103],"Blood":[104],"was":[105,142,152,162],"collected":[106],"weekly":[107],"measurement":[109],"anti-BSA":[111,187,243],"titer.":[112],"Hybridomas":[113],"prepared":[115],"from":[116],"mice":[117],"immunized":[118],"Scarlet":[120,136],"Macaw":[121,137],"(Ara":[122],"macao)":[123],"purified":[125],"by":[126,144],"salt":[127],"precipitation":[128],"gel":[130],"chromatography.":[131],"Specificity":[132],"other":[139],"species":[141],"confirmed":[143],"western":[147],"blot":[148],"analysis.":[149],"Hybridoma":[150],"HL544":[151,161],"cloned":[153],"antibody":[156,188,284,294],"purified.":[157],"Following":[158],"biotinylation,":[159],"incorporated":[163],"that":[167],"measured":[168],"BSA.":[175,198,251],"RESULTS:":[176],"Adult":[177],"Macaws":[181,240],"developed":[182,241],"strong":[183,242],"primary":[184],"secondary":[186,208,261],"titers":[189],"14":[190,215,269],"after":[192],"inoculation":[193,200,253],"An":[199,252],"interval":[201,212,254,266],"28":[203,256],"resulted":[205,258],"stronger":[207,260],"than":[210,263],"only":[214,268],"days.":[216,270],"CONCLUSIONS:":[217],"specific":[219],"immunoglobulins":[222],"can":[223],"be":[224,287],"used":[225],"humoral":[231],"immune":[232],"macaws.":[235],"titer":[244],"when":[245],"did":[264],"CLINICAL":[271],"RELEVANCE:":[272],"These":[273],"MAB,":[274],"first":[276],"reported":[277],"have":[279],"a":[282],"psittacine":[283,293,301],"class,":[285],"will":[286],"useful":[288],"evaluation":[291],"development":[299],"diagnostic":[302],"assays.":[303]},"counts_by_year":[{"year":2024,"cited_by_count":1},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}