{"id":"https://openalex.org/W96925745","doi":null,"title":"Role of Non-myocytes in Engineering of Highly Functional Pluripotent Stem Cell-derived Cardiac Tissues","display_name":"Role of Non-myocytes in Engineering of Highly Functional Pluripotent Stem Cell-derived Cardiac Tissues","publication_year":2013,"publication_date":"2013-01-01","ids":{"openalex":"https://openalex.org/W96925745","mag":"96925745"},"language":"en","primary_location":{"id":"pmh:oai:dukespace.lib.duke.edu:10161/7134","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/10161/7134","pdf_url":"http://hdl.handle.net/10161/7134","source":{"id":"https://openalex.org/S4306400687","display_name":"DukeSpace (Duke University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I170897317","host_organization_name":"Duke University","host_organization_lineage":["https://openalex.org/I170897317"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Dissertation"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"http://hdl.handle.net/10161/7134","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5082734561","display_name":"Brian Liau","orcid":"https://orcid.org/0000-0002-8233-771X","observed_orcids":["https://orcid.org/0000-0002-8233-771X"]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Brian Liau","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5082734561"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":1,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12264","display_name":"Tissue Engineering and Regenerative Medicine","score":0.576200008392334,"subfield":{"id":"https://openalex.org/subfields/2746","display_name":"Surgery"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T12264","display_name":"Tissue Engineering and Regenerative Medicine","score":0.576200008392334,"subfield":{"id":"https://openalex.org/subfields/2746","display_name":"Surgery"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10505","display_name":"Pluripotent Stem Cells Research","score":0.4032999873161316,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10217","display_name":"Cardiac electrophysiology and arrhythmias","score":0.00430000014603138,"subfield":{"id":"https://openalex.org/subfields/2705","display_name":"Cardiology and Cardiovascular Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/engineered-heart-tissue","display_name":"engineered heart tissue","score":0.6840000152587891},{"id":"https://openalex.org/keywords/pluripotent-stem-cells","display_name":"pluripotent stem cells","score":0.8889999985694885},{"id":"https://openalex.org/keywords/mouse-embryonic-stem-cells","display_name":"mouse embryonic stem cells","score":0.8970000147819519},{"id":"https://openalex.org/keywords/induced-pluripotent-stem-cells","display_name":"induced pluripotent stem cells","score":0.9900000095367432},{"id":"https://openalex.org/keywords/cardiomyocytes","display_name":"cardiomyocytes","score":0.8140000104904175},{"id":"https://openalex.org/keywords/cardiac-fibroblasts","display_name":"cardiac fibroblasts","score":0.9340000152587891},{"id":"https://openalex.org/keywords/cardiac-patch","display_name":"cardiac patch","score":0.39100000262260437},{"id":"https://openalex.org/keywords/microfabrication","display_name":"microfabrication","score":0.796999990940094},{"id":"https://openalex.org/keywords/electrical-coupling","display_name":"electrical coupling","score":0.6000000238418579},{"id":"https://openalex.org/keywords/action-potential-propagation","display_name":"action potential propagation","score":0.8299999833106995},{"id":"https://openalex.org/keywords/contractile-force","display_name":"contractile force","score":0.6759999990463257},{"id":"https://openalex.org/keywords/paracrine-signaling","display_name":"paracrine signaling","score":0.5759999752044678},{"id":"https://openalex.org/keywords/cardiomyocyte-differentiation","display_name":"cardiomyocyte differentiation","score":0.4690000116825104},{"id":"https://openalex.org/keywords/nkx2-5","display_name":"Nkx2.5","score":0.7210000157356262}],"concepts":[{"id":"https://openalex.org/C107459253","wikidata":"https://www.wikidata.org/wiki/Q674925","display_name":"Induced pluripotent stem cell","level":4,"score":0.8010300993919373},{"id":"https://openalex.org/C207200792","wikidata":"https://www.wikidata.org/wiki/Q428914","display_name":"Myocyte","level":2,"score":0.7050190567970276},{"id":"https://openalex.org/C28328180","wikidata":"https://www.wikidata.org/wiki/Q48196","display_name":"Stem cell","level":2,"score":0.6409177184104919},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.576602041721344},{"id":"https://openalex.org/C49892992","wikidata":"https://www.wikidata.org/wiki/Q1540285","display_name":"Tissue engineering","level":2,"score":0.44672006368637085},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.42948266863822937},{"id":"https://openalex.org/C169760540","wikidata":"https://www.wikidata.org/wiki/Q207011","display_name":"Neuroscience","level":1,"score":0.3650423288345337},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.34823077917099},{"id":"https://openalex.org/C145103041","wikidata":"https://www.wikidata.org/wiki/Q1151519","display_name":"Embryonic stem cell","level":3,"score":0.2016257643699646},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.11618101596832275},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:dukespace.lib.duke.edu:10161/7134","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/10161/7134","pdf_url":"http://hdl.handle.net/10161/7134","source":{"id":"https://openalex.org/S4306400687","display_name":"DukeSpace (Duke University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I170897317","host_organization_name":"Duke University","host_organization_lineage":["https://openalex.org/I170897317"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Dissertation"},{"id":"mag:96925745","doi":null,"is_oa":false,"landing_page_url":"https://dukespace.lib.duke.edu/dspace/handle/10161/7134","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"pmh:oai:dukespace.lib.duke.edu:10161/7134","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/10161/7134","pdf_url":"http://hdl.handle.net/10161/7134","source":{"id":"https://openalex.org/S4306400687","display_name":"DukeSpace (Duke University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I170897317","host_organization_name":"Duke University","host_organization_lineage":["https://openalex.org/I170897317"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Dissertation"},"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.8560000061988831,"id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W96925745.pdf","grobid_xml":"https://content.openalex.org/works/W96925745.grobid-xml"},"referenced_works_count":315,"referenced_works":["https://openalex.org/W53451770","https://openalex.org/W323411733","https://openalex.org/W1211474807","https://openalex.org/W1492344672","https://openalex.org/W1522672235","https://openalex.org/W1530324520","https://openalex.org/W1533279781","https://openalex.org/W1549569049","https://openalex.org/W1554555407","https://openalex.org/W1569560733","https://openalex.org/W1579094115","https://openalex.org/W1587419561","https://openalex.org/W1608875353","https://openalex.org/W1631250427","https://openalex.org/W1755721460","https://openalex.org/W1836520117","https://openalex.org/W1881358965","https://openalex.org/W1932855363","https://openalex.org/W1954362343","https://openalex.org/W1955971595","https://openalex.org/W1964046571","https://openalex.org/W1964152667","https://openalex.org/W1964525036","https://openalex.org/W1966563415","https://openalex.org/W1969038150","https://openalex.org/W1969832018","https://openalex.org/W1969904397","https://openalex.org/W1970102552","https://openalex.org/W1970516435","https://openalex.org/W1970947849","https://openalex.org/W1971630553","https://openalex.org/W1972659765","https://openalex.org/W1972662556","https://openalex.org/W1973557736","https://openalex.org/W1975292043","https://openalex.org/W1976419485","https://openalex.org/W1977152294","https://openalex.org/W1978146894","https://openalex.org/W1978186163","https://openalex.org/W1979868276","https://openalex.org/W1979941994","https://openalex.org/W1980141913","https://openalex.org/W1980846500","https://openalex.org/W1981531825","https://openalex.org/W1982210988","https://openalex.org/W1984052098","https://openalex.org/W1984550452","https://openalex.org/W1987153030","https://openalex.org/W1987690650","https://openalex.org/W1987774343","https://openalex.org/W1987868056","https://openalex.org/W1988451304","https://openalex.org/W1989481002","https://openalex.org/W1990810165","https://openalex.org/W1990930800","https://openalex.org/W1991237283","https://openalex.org/W1991421066","https://openalex.org/W1992545807","https://openalex.org/W1995282679","https://openalex.org/W1996163407","https://openalex.org/W1996196738","https://openalex.org/W1996994627","https://openalex.org/W1997404143","https://openalex.org/W1998642283","https://openalex.org/W1999881610","https://openalex.org/W2000168964","https://openalex.org/W2001045775","https://openalex.org/W2002970726","https://openalex.org/W2003035943","https://openalex.org/W2005586092","https://openalex.org/W2005840935","https://openalex.org/W2006728407","https://openalex.org/W2008031660","https://openalex.org/W2009110094","https://openalex.org/W2010177021","https://openalex.org/W2010417854","https://openalex.org/W2010847353","https://openalex.org/W2012681303","https://openalex.org/W2012774822","https://openalex.org/W2012852518","https://openalex.org/W2016242739","https://openalex.org/W2016976019","https://openalex.org/W2017852297","https://openalex.org/W2017885692","https://openalex.org/W2018071690","https://openalex.org/W2018463503","https://openalex.org/W2018941092","https://openalex.org/W2019037987","https://openalex.org/W2020312446","https://openalex.org/W2021024066","https://openalex.org/W2021458745","https://openalex.org/W2021586120","https://openalex.org/W2022675050","https://openalex.org/W2022759861","https://openalex.org/W2024763420","https://openalex.org/W2026632078","https://openalex.org/W2026789574","https://openalex.org/W2027016517","https://openalex.org/W2028163040","https://openalex.org/W2028163205","https://openalex.org/W2028735060","https://openalex.org/W2030258556","https://openalex.org/W2031217240","https://openalex.org/W2031450533","https://openalex.org/W2031550930","https://openalex.org/W2031775206","https://openalex.org/W2032310545","https://openalex.org/W2032341330","https://openalex.org/W2033033141","https://openalex.org/W2034238319","https://openalex.org/W2034454142","https://openalex.org/W2034543456","https://openalex.org/W2034757554","https://openalex.org/W2034862301","https://openalex.org/W2034998169","https://openalex.org/W2035708591","https://openalex.org/W2036272929","https://openalex.org/W2036876430","https://openalex.org/W2039187714","https://openalex.org/W2039580049","https://openalex.org/W2039628220","https://openalex.org/W2040099855","https://openalex.org/W2041797639","https://openalex.org/W2041897037","https://openalex.org/W2042560953","https://openalex.org/W2044459428","https://openalex.org/W2045048112","https://openalex.org/W2046951940","https://openalex.org/W2047427087","https://openalex.org/W2047654571","https://openalex.org/W2047908260","https://openalex.org/W2048255936","https://openalex.org/W2048410503","https://openalex.org/W2052318709","https://openalex.org/W2052959842","https://openalex.org/W2053669774","https://openalex.org/W2054706358","https://openalex.org/W2054850711","https://openalex.org/W2057005309","https://openalex.org/W2058247847","https://openalex.org/W2061653931","https://openalex.org/W2064124462","https://openalex.org/W2064193713","https://openalex.org/W2065066942","https://openalex.org/W2065164306","https://openalex.org/W2065168340","https://openalex.org/W2066525457","https://openalex.org/W2068135866","https://openalex.org/W2068524893","https://openalex.org/W2069802504","https://openalex.org/W2069940803","https://openalex.org/W2070158545","https://openalex.org/W2070424623","https://openalex.org/W2072686885","https://openalex.org/W2072740783","https://openalex.org/W2074431288","https://openalex.org/W2074505230","https://openalex.org/W2076600697","https://openalex.org/W2076812266","https://openalex.org/W2077717135","https://openalex.org/W2077806591","https://openalex.org/W2080085786","https://openalex.org/W2080539468","https://openalex.org/W2081862378","https://openalex.org/W2081878872","https://openalex.org/W2082900428","https://openalex.org/W2084334139","https://openalex.org/W2085376312","https://openalex.org/W2086836892","https://openalex.org/W2086980194","https://openalex.org/W2087750804","https://openalex.org/W2088320964","https://openalex.org/W2088513836","https://openalex.org/W2088658807","https://openalex.org/W2089780116","https://openalex.org/W2090353883","https://openalex.org/W2091013332","https://openalex.org/W2091574626","https://openalex.org/W2092575722","https://openalex.org/W2093171685","https://openalex.org/W2093459448","https://openalex.org/W2093497167","https://openalex.org/W2093592911","https://openalex.org/W2094097843","https://openalex.org/W2094457676","https://openalex.org/W2095683716","https://openalex.org/W2096237299","https://openalex.org/W2096650620","https://openalex.org/W2097246504","https://openalex.org/W2098470464","https://openalex.org/W2100491180","https://openalex.org/W2101228997","https://openalex.org/W2101514652","https://openalex.org/W2103572770","https://openalex.org/W2104697565","https://openalex.org/W2105127081","https://openalex.org/W2105178180","https://openalex.org/W2106279829","https://openalex.org/W2106586369","https://openalex.org/W2106791580","https://openalex.org/W2107666058","https://openalex.org/W2108083474","https://openalex.org/W2108285596","https://openalex.org/W2109249622","https://openalex.org/W2110162714","https://openalex.org/W2110275759","https://openalex.org/W2110458816","https://openalex.org/W2110614144","https://openalex.org/W2112091653","https://openalex.org/W2114288259","https://openalex.org/W2114853779","https://openalex.org/W2116041125","https://openalex.org/W2116419097","https://openalex.org/W2116953529","https://openalex.org/W2118030557","https://openalex.org/W2118221757","https://openalex.org/W2118478353","https://openalex.org/W2120693516","https://openalex.org/W2120742544","https://openalex.org/W2120949673","https://openalex.org/W2121621123","https://openalex.org/W2122106267","https://openalex.org/W2123163588","https://openalex.org/W2124066511","https://openalex.org/W2125449435","https://openalex.org/W2125931106","https://openalex.org/W2125987139","https://openalex.org/W2128085444","https://openalex.org/W2129118672","https://openalex.org/W2129328611","https://openalex.org/W2129460482","https://openalex.org/W2129956520","https://openalex.org/W2130334101","https://openalex.org/W2130633620","https://openalex.org/W2131388270","https://openalex.org/W2132589089","https://openalex.org/W2134053621","https://openalex.org/W2134506355","https://openalex.org/W2134704099","https://openalex.org/W2134982681","https://openalex.org/W2135655740","https://openalex.org/W2136070250","https://openalex.org/W2136071912","https://openalex.org/W2137898197","https://openalex.org/W2138137694","https://openalex.org/W2138977668","https://openalex.org/W2139109611","https://openalex.org/W2139565971","https://openalex.org/W2139837799","https://openalex.org/W2139949573","https://openalex.org/W2140165420","https://openalex.org/W2140609834","https://openalex.org/W2141126811","https://openalex.org/W2141172232","https://openalex.org/W2141200068","https://openalex.org/W2142046859","https://openalex.org/W2143229579","https://openalex.org/W2143814603","https://openalex.org/W2145172444","https://openalex.org/W2145674044","https://openalex.org/W2147687016","https://openalex.org/W2149016416","https://openalex.org/W2149069089","https://openalex.org/W2150428021","https://openalex.org/W2150501736","https://openalex.org/W2151497368","https://openalex.org/W2151722542","https://openalex.org/W2151878992","https://openalex.org/W2152043096","https://openalex.org/W2152658552","https://openalex.org/W2153099809","https://openalex.org/W2154070829","https://openalex.org/W2154150229","https://openalex.org/W2154279806","https://openalex.org/W2154873274","https://openalex.org/W2155347215","https://openalex.org/W2155363902","https://openalex.org/W2156407866","https://openalex.org/W2156796692","https://openalex.org/W2157369309","https://openalex.org/W2157409331","https://openalex.org/W2157647701","https://openalex.org/W2157700111","https://openalex.org/W2157979094","https://openalex.org/W2158197581","https://openalex.org/W2158470819","https://openalex.org/W2158644069","https://openalex.org/W2158979977","https://openalex.org/W2159918055","https://openalex.org/W2161118621","https://openalex.org/W2162711858","https://openalex.org/W2162857699","https://openalex.org/W2163068618","https://openalex.org/W2163388642","https://openalex.org/W2163442684","https://openalex.org/W2165027208","https://openalex.org/W2165677217","https://openalex.org/W2166388268","https://openalex.org/W2167202160","https://openalex.org/W2167566197","https://openalex.org/W2167897928","https://openalex.org/W2168245648","https://openalex.org/W2169755701","https://openalex.org/W2169859308","https://openalex.org/W2170675143","https://openalex.org/W2170871745","https://openalex.org/W2171547034","https://openalex.org/W2172011106","https://openalex.org/W2182141197","https://openalex.org/W2294525956","https://openalex.org/W2315194766","https://openalex.org/W2325705904","https://openalex.org/W2337503547","https://openalex.org/W2418527466","https://openalex.org/W2547125981"],"related_works":["https://openalex.org/W2104697565","https://openalex.org/W2027069914","https://openalex.org/W3137738173","https://openalex.org/W2658673161","https://openalex.org/W2073488181","https://openalex.org/W2768631248","https://openalex.org/W2114288259","https://openalex.org/W3203427026","https://openalex.org/W3128473682","https://openalex.org/W2044934149"],"abstract_inverted_index":{"Massive":[0],"loss":[1],"of":[2,8,40,77,83,127,139,169,235,243,259,284,295,312,386,407,420,427,447,478,543,562],"cardiac":[3,85,118,153,170,217,237,256,297,381,387,391,409,418,442,465,496,546,568],"tissue":[4,111,211],"as":[5,431,433],"a":[6,13,97,146,209,233,301,397],"result":[7],"myocardial":[9,124],"infarction":[10],"can":[11],"create":[12,215],"poorly-conducting":[14],"substrate":[15],"with":[16,37,220,357,362,369,440,463,472],"impaired":[17],"contractility,":[18],"ultimately":[19],"leading":[20],"to":[21,50,114,144,148,214,239,286,291,323,349,366,393,492],"heart":[22],"failure":[23],"and":[24,53,60,75,102,107,132,195,250,265,269,280,318,333,453,476,483,506,513,538,555,565,572],"lethal":[25],"arrhythmias.":[26],"Recent":[27],"advances":[28],"in":[29,57,202,229,321,364,412,424,434,509],"pluripotent":[30,157,303,335,549],"stem":[31,158,175,304,331,336,400,550],"cell":[32,68,90,204,223,355,551],"research":[33],"have":[34,64],"provided":[35],"investigators":[36],"potent":[38],"sources":[39],"cardiogenic":[41],"cells":[42,176,320,332,338],"that":[43,66,189,254,360,490,502,527],"may":[44,87,528],"be":[45,394],"transplanted":[46],"into":[47,120,314],"failing":[48],"hearts":[49],"provide":[51,88,557],"electrical":[52,130,193,249],"mechanical":[54,251],"support.":[55],"Experiments":[56],"both":[58],"small":[59],"large":[61],"animal":[62],"models":[63],"shown":[65],"standard":[67],"delivery":[69],"techniques":[70,554],"suffer":[71],"from":[72,156,172,328,396,548],"poor":[73],"retention":[74,91],"engraftment":[76],"cells.":[78,159],"In":[79,532],"contrast,":[80],"the":[81,93,241,293,384,425,516],"transplantation":[82],"engineered":[84,152,216,245,255,380,390,414,511],"tissues":[86,125,154,218,382,392,415,512,547,569],"improved":[89,361],"at":[92],"injury":[94],"site,":[95],"creating":[96],"more":[98,104],"localized":[99],"paracrine":[100,500,524],"effect":[101],"yielding":[103],"efficient":[105],"structural":[106],"functional":[108,151,378,468,545],"repair.":[109],"However,":[110,372],"engineering":[112,212,561],"methodologies":[113],"assemble":[115],"cardiomyocytes":[116],"or":[117],"progenitors":[119,309],"aligned,":[121,262],"3-dimensional":[122],"(3D)":[123],"capable":[126,311,446],"physiologically":[128],"relevant":[129],"conduction":[131,274],"force":[133,282,458],"generation":[134,459,542],"are":[135],"lacking.":[136],"The":[137],"objective":[138],"this":[140,162,530,534],"thesis":[141,535],"was":[142],"thus":[143],"develop":[145],"methodology":[147],"generate":[149],"highly":[150,263,353,377,544],"starting":[155],"To":[160],"accomplish":[161],"goal,":[163],"we":[164,289,404],"first":[165],"derived":[166,327],"purified":[167],"populations":[168],"myocytes":[171],"mouse":[173,329],"embryonic":[174,330],"(mESC-CMs)":[177],"by":[178,182,299,339,343,416],"antibiotic":[179,340],"selection":[180,341],"driven":[181,342],"an":[183,344],"\u03b1-myosin":[184],"heavy-chain":[185],"promoter.":[186],"Culture":[187],"conditions":[188],"yielded":[190],"robust":[191],"mESC-CM":[192,474,504],"coupling":[194],"fast":[196],"action":[197,272,450],"potential":[198,273,451],"propagation":[199,452],"were":[200,326,445,470],"optimized":[201],"confluent":[203],"monolayers.":[205],"We":[206,225],"then":[207],"developed":[208],"microfabrication-based":[210],"approach":[213],"(\"patches\")":[219],"uniform":[221],"3D":[222,244,379,413,429,510],"alignment.":[224],"found":[226],"that,":[227],"unlike":[228,373],"monolayers,":[230],"mESC-CMs":[231,268,444],"required":[232],"population":[234],"supporting":[236],"fibroblasts":[238,298,419,497],"enable":[240],"formation":[242],"tissues.":[246],"Detailed":[247],"structural,":[248],"characterization":[252],"demonstrated":[253],"patches":[257],"consisted":[258],"dense,":[260],"uniformly":[261],"differentiated":[264,354],"electromechanically":[266],"coupled":[267],"supported":[270],"rapid":[271],"velocities":[275],"between":[276],"22":[277],"-":[278],"25cm/s":[279],"contractile":[281,457,484],"amplitudes":[283],"up":[285],"2mN.":[287],"Next,":[288],"sought":[290],"circumvent":[292],"use":[294],"primary":[296],"utilizing":[300],"single":[302],"cell-derived":[305,401],"source,":[306],"multipotent":[307],"cardiovascular":[308],"(CVPs)":[310],"differentiating":[313],"vascular":[315],"smooth":[316],"muscle":[317],"endothelial":[319],"addition":[322,385],"cardiomyocytes.":[324],"CVPs":[325,351,375],"induced":[334],"(iPS)":[337],"Nkx2-5":[345],"enhancer":[346],"element.":[347],"Similar":[348],"mESC-CMs,":[350,374],"formed":[352,376,395],"monolayers":[356],"electrophysiological":[358],"properties":[359],"time":[363],"culture":[365],"levels":[367],"achieved":[368],"pure":[370],"mESC-CMs.":[371],"without":[383],"fibroblasts,":[388,443,494],"enabling":[389],"single,":[398],"entirely":[399],"source.":[402],"Finally,":[403],"explored":[405],"mechanisms":[406,540],"synergistic":[408],"fibroblast/myocyte":[410],"signaling":[411],"using":[417],"different":[421],"developmental":[422],"stages":[423],"settings":[426],"direct":[428],"co-culture":[430],"well":[432],"conditioned":[435],"media":[436],"studies.":[437],"When":[438],"co-cultured":[439,462],"fetal":[441,495],"two-fold":[448],"faster":[449],"1.5-fold":[454],"higher":[455],"maximum":[456],"than":[460],"when":[461],"adult":[464,493],"fibroblasts.":[466],"These":[467,553],"improvements":[469],"associated":[471],"enhanced":[473],"spreading":[475,505],"upregulation":[477],"important":[479],"ion":[480],"channel,":[481],"coupling,":[482],"proteins.":[485],"Conditioned":[486],"medium":[487],"studies":[488],"revealed":[489,523],"compared":[491],"secreted":[498],"distinct":[499],"factors":[501],"promoted":[503],"spontaneous":[507],"contractility":[508],"acted":[514],"via":[515],"MEK-ERK":[517],"pathway.":[518],"Quantitative":[519],"gene":[520],"expression":[521],"analysis":[522],"factor":[525],"candidates":[526],"mediate":[529],"action.":[531],"summary,":[533],"presents":[536],"methods":[537],"underlying":[539],"for":[541,559,570],"sources.":[552],"findings":[556],"foundation":[558],"future":[560],"human":[563],"ES":[564],"iPS":[566],"cell-based":[567],"therapeutic":[571],"drug":[573],"screening":[574],"applications.":[575]},"counts_by_year":[{"year":2022,"cited_by_count":1}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2016-06-24T00:00:00"}
