{"id":"https://openalex.org/W968390735","doi":"https://doi.org/10.4049/jimmunol.182.supp.33.13","title":"Real-time imaging of TCR dynamics in vivo reveals rapid TCR clustering and internalization in response to antigen (33.13)","display_name":"Real-time imaging of TCR dynamics in vivo reveals rapid TCR clustering and internalization in response to antigen (33.13)","publication_year":2009,"publication_date":"2009-04-01","ids":{"openalex":"https://openalex.org/W968390735","doi":"https://doi.org/10.4049/jimmunol.182.supp.33.13","mag":"968390735"},"language":"en","primary_location":{"id":"doi:10.4049/jimmunol.182.supp.33.13","doi":"https://doi.org/10.4049/jimmunol.182.supp.33.13","is_oa":false,"landing_page_url":"https://doi.org/10.4049/jimmunol.182.supp.33.13","pdf_url":null,"source":{"id":"https://openalex.org/S38008053","display_name":"The Journal of Immunology","issn_l":"0022-1767","issn":["0022-1767","1550-6606"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4320800377","host_organization_name":"American Association of Immunologists","host_organization_lineage":["https://openalex.org/P4320800377"],"host_organization_lineage_names":["American Association of Immunologists"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of Immunology","raw_type":"journal-article"},"volume":null,"type":"conference-abstract","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5027358643","display_name":"Rachel Sharon Friedman","orcid":"https://orcid.org/0000-0001-7549-6387","observed_orcids":["https://orcid.org/0000-0001-7549-6387"]},"institutions":[{"id":"https://openalex.org/I180670191","display_name":"University of California, San Francisco","ror":"https://ror.org/043mz5j54","country_code":"US","type":"education","lineage":["https://openalex.org/I180670191"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Rachel S Friedman","raw_affiliation_strings":["Department of Pathology, University of California, San Francisco , San Francisco, CA","1Department of Pathology, University of California, San Francisco, San Francisco, CA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pathology, University of California, San Francisco , San Francisco, CA","institution_ids":["https://openalex.org/I180670191"]},{"raw_affiliation_string":"1Department of Pathology, University of California, San Francisco, San Francisco, CA","institution_ids":["https://openalex.org/I180670191"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5063202361","display_name":"Peter Beemiller","orcid":"https://orcid.org/0000-0002-2736-3405","observed_orcids":["https://orcid.org/0000-0002-2736-3405"]},"institutions":[{"id":"https://openalex.org/I180670191","display_name":"University of California, San Francisco","ror":"https://ror.org/043mz5j54","country_code":"US","type":"education","lineage":["https://openalex.org/I180670191"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Peter J Beemiller","raw_affiliation_strings":["Department of Pathology, University of California, San Francisco , San Francisco, CA","1Department of Pathology, University of California, San Francisco, San Francisco, CA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pathology, University of California, San Francisco , San Francisco, CA","institution_ids":["https://openalex.org/I180670191"]},{"raw_affiliation_string":"1Department of Pathology, University of California, San Francisco, San Francisco, CA","institution_ids":["https://openalex.org/I180670191"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5008625070","display_name":"Caitlin M. Sorensen","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I180670191","display_name":"University of California, San Francisco","ror":"https://ror.org/043mz5j54","country_code":"US","type":"education","lineage":["https://openalex.org/I180670191"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Caitlin M Sorensen","raw_affiliation_strings":["Department of Pathology, University of California, San Francisco , San Francisco, CA","1Department of Pathology, University of California, San Francisco, San Francisco, CA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pathology, University of California, San Francisco , San Francisco, CA","institution_ids":["https://openalex.org/I180670191"]},{"raw_affiliation_string":"1Department of Pathology, University of California, San Francisco, San Francisco, CA","institution_ids":["https://openalex.org/I180670191"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5018179968","display_name":"Matthew F. Krummel","orcid":"https://orcid.org/0000-0001-7915-3533","observed_orcids":["https://orcid.org/0000-0001-7915-3533"]},"institutions":[{"id":"https://openalex.org/I180670191","display_name":"University of California, San Francisco","ror":"https://ror.org/043mz5j54","country_code":"US","type":"education","lineage":["https://openalex.org/I180670191"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Matthew F Krummel","raw_affiliation_strings":["Department of Pathology, University of California, San Francisco , San Francisco, CA","1Department of Pathology, University of California, San Francisco, San Francisco, CA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pathology, University of California, San Francisco , San Francisco, CA","institution_ids":["https://openalex.org/I180670191"]},{"raw_affiliation_string":"1Department of Pathology, University of California, San Francisco, San Francisco, CA","institution_ids":["https://openalex.org/I180670191"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I180670191"],"apc_list":{"value":3500,"currency":"USD","value_usd":3500},"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":"182","issue":"Supplement_1","first_page":"33.13","last_page":"33.13"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10031","display_name":"T-cell and B-cell Immunology","score":0.9726999998092651,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10031","display_name":"T-cell and B-cell Immunology","score":0.9726999998092651,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10540","display_name":"Advanced Fluorescence Microscopy Techniques","score":0.006800000090152025,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10617","display_name":"Cellular transport and secretion","score":0.005400000140070915,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/tcr-clustering","display_name":"TCR clustering","score":0.9629999995231628},{"id":"https://openalex.org/keywords/naive-t-cells","display_name":"naive T cells","score":0.8330000042915344},{"id":"https://openalex.org/keywords/t-cell-activation","display_name":"T cell activation","score":0.8399999737739563},{"id":"https://openalex.org/keywords/dendritic-cells","display_name":"dendritic cells","score":0.8510000109672546},{"id":"https://openalex.org/keywords/antigen-recognition","display_name":"antigen recognition","score":0.7509999871253967},{"id":"https://openalex.org/keywords/lymph-nodes","display_name":"lymph nodes","score":0.8190000057220459},{"id":"https://openalex.org/keywords/t-cell-signaling","display_name":"T cell signaling","score":0.3970000147819519},{"id":"https://openalex.org/keywords/live-cell-imaging","display_name":"live-cell imaging","score":0.48100000619888306}],"concepts":[{"id":"https://openalex.org/C19317047","wikidata":"https://www.wikidata.org/wiki/Q412037","display_name":"T-cell receptor","level":4,"score":0.9223132729530334},{"id":"https://openalex.org/C139770010","wikidata":"https://www.wikidata.org/wiki/Q1339807","display_name":"Internalization","level":3,"score":0.7909402847290039},{"id":"https://openalex.org/C147483822","wikidata":"https://www.wikidata.org/wiki/Q103537","display_name":"Antigen","level":2,"score":0.6219075918197632},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.6204484701156616},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5511760115623474},{"id":"https://openalex.org/C2776090121","wikidata":"https://www.wikidata.org/wiki/Q193529","display_name":"T cell","level":3,"score":0.5387943387031555},{"id":"https://openalex.org/C114684123","wikidata":"https://www.wikidata.org/wiki/Q419458","display_name":"CD3","level":4,"score":0.5132615566253662},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.3967639207839966},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.2997451722621918},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.24159109592437744},{"id":"https://openalex.org/C167672396","wikidata":"https://www.wikidata.org/wiki/Q417800","display_name":"CD8","level":3,"score":0.1723509132862091},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.14251837134361267},{"id":"https://openalex.org/C8891405","wikidata":"https://www.wikidata.org/wiki/Q1059","display_name":"Immune system","level":2,"score":0.11235901713371277}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.4049/jimmunol.182.supp.33.13","doi":"https://doi.org/10.4049/jimmunol.182.supp.33.13","is_oa":false,"landing_page_url":"https://doi.org/10.4049/jimmunol.182.supp.33.13","pdf_url":null,"source":{"id":"https://openalex.org/S38008053","display_name":"The Journal of Immunology","issn_l":"0022-1767","issn":["0022-1767","1550-6606"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4320800377","host_organization_name":"American Association of Immunologists","host_organization_lineage":["https://openalex.org/P4320800377"],"host_organization_lineage_names":["American Association of Immunologists"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of Immunology","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2051609456","https://openalex.org/W2123478110","https://openalex.org/W4236222328","https://openalex.org/W2052573176","https://openalex.org/W2167670122","https://openalex.org/W7060686248","https://openalex.org/W4362455221","https://openalex.org/W1969675640","https://openalex.org/W4247575208","https://openalex.org/W2051178853"],"abstract_inverted_index":{"Abstract":[0],"Real-time":[1],"in":[2,25,36,64,68,182,191],"vivo":[3],"imaging":[4,28],"has":[5,33],"given":[6],"insight":[7],"into":[8],"the":[9,69,110,118,122,128,183,189,216],"cellular":[10],"dynamics":[11,24,63],"of":[12,29,47,52,109,120,138,148],"T":[13,59,66,111,139,152,180],"cell":[14,60,129],"activation,":[15],"but":[16],"less":[17],"is":[18],"known":[19],"about":[20],"receptor":[21,61],"and":[22,49,99,102,156,170,201,209,221],"signaling":[23,31],"vivo.":[26],"Live":[27],"subcellular":[30],"complexes":[32],"been":[34],"challenging":[35],"living":[37],"tissues":[38],"due":[39],"to":[40,57,96,113,127,193,205],"low":[41],"physiological":[42],"molecular":[43],"densities,":[44],"inherent":[45],"limitations":[46],"fluorophores,":[48],"strong":[50],"autofluorescence":[51],"tissues.":[53],"Our":[54],"goal":[55],"was":[56,124,166,213],"characterize":[58],"(TCR)":[62],"nai\u0308ve":[65,179],"cells":[67,78,112,140,153,181],"lymph":[70,184],"node":[71,185],"during":[72],"their":[73,143,158,194],"first":[74,195],"encounter":[75],"with":[76,197],"dendritic":[77],"(DCs)":[79],"bearing":[80,199],"cognate":[81],"antigen.":[82],"To":[83],"do":[84],"so":[85],"we":[86],"developed":[87],"a":[88,91,135],"mouse":[89],"expressing":[90],"GFP":[92],"tagged":[93],"OT-I":[94],"TCR":[95,123,163,172,175,190,207],"track":[97],"surface":[98,130],"intracellular":[100],"TCR,":[101],"used":[103],"image":[104],"processing":[105],"facilitated":[106],"by":[107,215],"co-labeling":[108],"isolate":[114],"specific":[115],"fluorescence.":[116],"In":[117],"absence":[119],"antigen,":[121],"mainly":[125],"confined":[126],"without":[131],"consistent":[132],"polarity;":[133],"however,":[134],"small":[136],"percentage":[137],"transiently":[141],"clustered":[142,157],"TCR.":[144,159],"Within":[145],"30":[146],"min":[147],"antigen":[149,200],"recognition,":[150],"many":[151],"stopped":[154],"crawling":[155],"Surprisingly,":[160],"detectable":[161],"antigen-dependent":[162],"clustering":[164,173,208],"duration":[165],"short":[167],"(7.1\u00b15.1":[168],"min)":[169],"as":[171],"declined,":[174],"internalization":[176],"increased.":[177],"Thus,":[178],"can":[186],"signal":[187],"through":[188],"response":[192],"interaction":[196],"DCs":[198],"these":[202],"contacts":[203],"lead":[204],"rapid":[206],"internalization.":[210],"This":[211],"work":[212],"funded":[214],"Larry":[217],"L.":[218],"Hillblom":[219],"Foundation":[220],"NIH":[222],"R21.":[223]},"counts_by_year":[],"updated_date":"2026-09-22T06:50:58.402377","created_date":"2016-06-24T00:00:00"}