{"id":"https://openalex.org/W964924809","doi":null,"title":"Homogeneous, bioluminescent assays for proteasome activity","display_name":"Homogeneous, bioluminescent assays for proteasome activity","publication_year":2006,"publication_date":"2006-04-15","ids":{"openalex":"https://openalex.org/W964924809","mag":"964924809"},"language":"en","primary_location":{"id":"mag:964924809","doi":null,"is_oa":false,"landing_page_url":"https://cancerres.aacrjournals.org/content/66/8_Supplement/321.3","pdf_url":null,"source":{"id":"https://openalex.org/S168522863","display_name":"Cancer Research","issn_l":"0008-5472","issn":["0008-5472","1538-7445"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320273","host_organization_name":"American Association for Cancer Research","host_organization_lineage":["https://openalex.org/P4310320273"],"host_organization_lineage_names":["American Association for Cancer Research"],"type":"journal"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"Cancer Research","raw_type":null},"volume":null,"type":"article","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5113626485","display_name":"Martha A. O\u2019Brien","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I199117387","display_name":"Promega (United States)","ror":"https://ror.org/01dra3713","country_code":"US","type":"company","lineage":["https://openalex.org/I199117387"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Martha A. O\u2019Brien","raw_affiliation_strings":["Promega Corporation, Madison, WI and Promega Biosciences, San Luis Obispo, CA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Promega Corporation, Madison, WI and Promega Biosciences, San Luis Obispo, CA","institution_ids":["https://openalex.org/I199117387"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5074524470","display_name":"Richard A Moravec","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I199117387","display_name":"Promega (United States)","ror":"https://ror.org/01dra3713","country_code":"US","type":"company","lineage":["https://openalex.org/I199117387"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Richard A Moravec","raw_affiliation_strings":["Promega Corporation, Madison, WI and Promega Biosciences, San Luis Obispo, CA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Promega Corporation, Madison, WI and Promega Biosciences, San Luis Obispo, CA","institution_ids":["https://openalex.org/I199117387"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5003419486","display_name":"Terry Riss","orcid":"https://orcid.org/0000-0002-9130-2084","observed_orcids":["https://orcid.org/0000-0002-9130-2084"]},"institutions":[{"id":"https://openalex.org/I199117387","display_name":"Promega (United States)","ror":"https://ror.org/01dra3713","country_code":"US","type":"company","lineage":["https://openalex.org/I199117387"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Terry Riss","raw_affiliation_strings":["Promega Corporation, Madison, WI and Promega Biosciences, San Luis Obispo, CA"],"raw_orcid":"https://orcid.org/0000-0002-9130-2084","affiliations":[{"raw_affiliation_string":"Promega Corporation, Madison, WI and Promega Biosciences, San Luis Obispo, CA","institution_ids":["https://openalex.org/I199117387"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5035955014","display_name":"Michael A. Scurria","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I199117387","display_name":"Promega (United States)","ror":"https://ror.org/01dra3713","country_code":"US","type":"company","lineage":["https://openalex.org/I199117387"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Michael A. Scurria","raw_affiliation_strings":["Promega Corporation, Madison, WI and Promega Biosciences, San Luis Obispo, CA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Promega Corporation, Madison, WI and Promega Biosciences, San Luis Obispo, CA","institution_ids":["https://openalex.org/I199117387"]}]},{"author_position":"middle","author":{"id":null,"display_name":"William J. Daily","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I199117387","display_name":"Promega (United States)","ror":"https://ror.org/01dra3713","country_code":"US","type":"company","lineage":["https://openalex.org/I199117387"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"William J. Daily","raw_affiliation_strings":["Promega Corporation, Madison, WI and Promega Biosciences, San Luis Obispo, CA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Promega Corporation, Madison, WI and Promega Biosciences, San Luis Obispo, CA","institution_ids":["https://openalex.org/I199117387"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5061374782","display_name":"Dieter H. Klaubert","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I199117387","display_name":"Promega (United States)","ror":"https://ror.org/01dra3713","country_code":"US","type":"company","lineage":["https://openalex.org/I199117387"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Dieter H. Klaubert","raw_affiliation_strings":["Promega Corporation, Madison, WI and Promega Biosciences, San Luis Obispo, CA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Promega Corporation, Madison, WI and Promega Biosciences, San Luis Obispo, CA","institution_ids":["https://openalex.org/I199117387"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5041489098","display_name":"Keith V. Wood","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I199117387","display_name":"Promega (United States)","ror":"https://ror.org/01dra3713","country_code":"US","type":"company","lineage":["https://openalex.org/I199117387"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Keith V. Wood","raw_affiliation_strings":["Promega Corporation, Madison, WI and Promega Biosciences, San Luis Obispo, CA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Promega Corporation, Madison, WI and Promega Biosciences, San Luis Obispo, CA","institution_ids":["https://openalex.org/I199117387"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5112016242","display_name":"Robert F. Bulleit","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I199117387","display_name":"Promega (United States)","ror":"https://ror.org/01dra3713","country_code":"US","type":"company","lineage":["https://openalex.org/I199117387"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Robert F. Bulleit","raw_affiliation_strings":["Promega Corporation, Madison, WI and Promega Biosciences, San Luis Obispo, CA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Promega Corporation, Madison, WI and Promega Biosciences, San Luis Obispo, CA","institution_ids":["https://openalex.org/I199117387"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I199117387"],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.0,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"66","issue":null,"first_page":"321","last_page":"321"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11041","display_name":"Ubiquitin and proteasome pathways","score":0.9003999829292297,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11041","display_name":"Ubiquitin and proteasome pathways","score":0.9003999829292297,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12561","display_name":"bioluminescence and chemiluminescence research","score":0.0803999975323677,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10620","display_name":"Protease and Inhibitor Mechanisms","score":0.006300000008195639,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/proteasome-activity","display_name":"proteasome activity","score":0.8659999966621399},{"id":"https://openalex.org/keywords/bioluminescence-assay","display_name":"bioluminescence assay","score":0.9010000228881836},{"id":"https://openalex.org/keywords/caspase-like-activity","display_name":"caspase-like activity","score":0.7689999938011169},{"id":"https://openalex.org/keywords/chymotrypsin-like-activity","display_name":"chymotrypsin-like activity","score":0.9879999756813049},{"id":"https://openalex.org/keywords/trypsin-like-activity","display_name":"trypsin-like activity","score":0.9919999837875366},{"id":"https://openalex.org/keywords/membrane-permeabilization","display_name":"membrane permeabilization","score":0.9129999876022339},{"id":"https://openalex.org/keywords/high-throughput-screening","display_name":"high-throughput screening","score":0.7960000038146973},{"id":"https://openalex.org/keywords/proteasome-inhibitors","display_name":"proteasome inhibitors","score":0.9129999876022339},{"id":"https://openalex.org/keywords/bortezomib","display_name":"bortezomib","score":0.9679999947547913},{"id":"https://openalex.org/keywords/lactacystin","display_name":"lactacystin","score":0.7360000014305115},{"id":"https://openalex.org/keywords/ubiquitin-proteasome-system","display_name":"ubiquitin-proteasome system","score":0.8320000171661377}],"concepts":[{"id":"https://openalex.org/C27740335","wikidata":"https://www.wikidata.org/wiki/Q14878333","display_name":"Proteasome","level":2,"score":0.8547966480255127},{"id":"https://openalex.org/C2777478702","wikidata":"https://www.wikidata.org/wiki/Q419319","display_name":"Bortezomib","level":3,"score":0.6615554690361023},{"id":"https://openalex.org/C2778367456","wikidata":"https://www.wikidata.org/wiki/Q7251235","display_name":"Proteasome inhibitor","level":3,"score":0.6068973541259766},{"id":"https://openalex.org/C111335760","wikidata":"https://www.wikidata.org/wiki/Q95385166","display_name":"Luciferase","level":4,"score":0.4908776879310608},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.440750390291214},{"id":"https://openalex.org/C2781307694","wikidata":"https://www.wikidata.org/wiki/Q33123","display_name":"Proteolysis","level":3,"score":0.44030240178108215},{"id":"https://openalex.org/C53227056","wikidata":"https://www.wikidata.org/wiki/Q189152","display_name":"Viability assay","level":3,"score":0.4394649565219879},{"id":"https://openalex.org/C2992195973","wikidata":"https://www.wikidata.org/wiki/Q33123","display_name":"Protein degradation","level":2,"score":0.4237709939479828},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.39093536138534546},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3480255603790283},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.33238619565963745},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.29359257221221924},{"id":"https://openalex.org/C2776364478","wikidata":"https://www.wikidata.org/wiki/Q467635","display_name":"Multiple myeloma","level":2,"score":0.2417106330394745},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.19964897632598877},{"id":"https://openalex.org/C54009773","wikidata":"https://www.wikidata.org/wiki/Q1429031","display_name":"Transfection","level":3,"score":0.17665481567382812},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.08979231119155884},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"mag:964924809","doi":null,"is_oa":false,"landing_page_url":"https://cancerres.aacrjournals.org/content/66/8_Supplement/321.3","pdf_url":null,"source":{"id":"https://openalex.org/S168522863","display_name":"Cancer Research","issn_l":"0008-5472","issn":["0008-5472","1538-7445"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320273","host_organization_name":"American Association for Cancer Research","host_organization_lineage":["https://openalex.org/P4310320273"],"host_organization_lineage_names":["American Association for Cancer Research"],"type":"journal"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"Cancer Research","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2406359225","https://openalex.org/W2030377061","https://openalex.org/W8110159","https://openalex.org/W7099482425","https://openalex.org/W2051270450","https://openalex.org/W1531381880","https://openalex.org/W2140082316","https://openalex.org/W2012855870","https://openalex.org/W2357900105","https://openalex.org/W2358844839"],"abstract_inverted_index":{"Proc":[0],"Amer":[1],"Assoc":[2],"Cancer":[3],"Res,":[4],"Volume":[5],"47,":[6],"2006\n\n1361":[7],"\n\nProtein":[8],"degradation":[9,25],"is":[10,55,248],"mediated":[11],"predominantly":[12],"through":[13],"the":[14,21,38,41,61,144,151,167,173,199],"ubiquitin":[15],"proteasome":[16,22,42,52,75,134,222,245,255],"pathway.":[17],"The":[18,49,154,210,230,254,275,296],"importance":[19],"of":[20,26,40,63,150,221],"in":[23,29,202,224,259,270],"regulating":[24],"proteins":[27],"involved":[28],"cell":[30,237],"cycle":[31],"control,":[32],"apoptosis,":[33],"and":[34,84,111,124,140,147,161,180,218,239,252,263,266,272,302],"angiogenesis":[35],"led":[36],"to":[37,91,142,166,194,243],"recognition":[39],"as":[43,71],"a":[44,102,112,195,205,225,260],"therapeutic":[45],"target":[46],"for":[47,60,73,88,120,131,186,293,307],"cancer.":[48],"first":[50,116],"generation":[51],"inhibitor,":[53],"bortezomib,":[54],"now":[56,126],"an":[57],"approved":[58],"drug":[59],"treatment":[62],"multiple":[64],"myeloma.":[65],"Peptide-conjugated":[66],"fluorophores":[67],"are":[68,257],"widely":[69],"used":[70],"substrates":[72,79,110,292],"monitoring":[74,121],"activity,":[76],"but":[77],"fluorogenic":[78,169,288],"can":[80,85],"exhibit":[81],"significant":[82],"background":[83],"be":[86],"problematic":[87],"screening":[89],"due":[90],"interference":[92],"from":[93],"test":[94],"compounds.":[95],"To":[96],"address":[97],"these":[98],"issues,":[99],"we":[100],"developed":[101,117,127],"homogeneous,":[103,128,276],"bioluminescent":[104,129],"method":[105,119],"that":[106,247],"combines":[107],"peptide-conjugated":[108],"aminoluciferin":[109],"stabilized":[113],"luciferase.":[114],"We":[115,136,189],"this":[118,192],"caspase":[122],"activities":[123,149],"have":[125,190,267],"assays":[130,156,175,256,289,305],"all":[132],"three":[133],"activities.":[135],"use":[137],"Suc-LLVY-aminoluciferin,":[138],"Z-LRR-aminoluciferin,":[139],"Z-nLPnLD-aminoluciferin":[141],"monitor":[143,244],"chymotrypsin-like,":[145],"trypsin-like,":[146],"caspase-like":[148],"proteasome,":[152],"respectively.":[153],"luminogenic":[155,277],"showed":[157],"increased":[158],"dynamic":[159],"range":[160],"improved":[162],"detection":[163],"sensitivity":[164],"compared":[165],"analogous":[168],"assays.":[170],"In":[171],"addition,":[172],"luminescent":[174],"rapidly":[176],"attained":[177],"maximum":[178],"signal":[179],"held":[181],"stable":[182],"light":[183],"emission,":[184],"allowing":[185],"assay":[187,197,212,231,279],"flexibility.":[188],"applied":[191],"technology":[193],"cell-based":[196,211],"using":[198,290],"Suc-LLVY-aminoluciferin":[200],"substrate":[201],"combination":[203],"with":[204],"selective":[206],"membrane":[207],"permeabilization":[208],"step.":[209],"does":[213],"not":[214],"require":[215],"lysate":[216],"preparation":[217],"enables":[219,300],"measurement":[220],"activity":[223,246],"more":[226,285],"physiologically":[227],"relevant":[228],"environment.":[229],"has":[232,240],"been":[233,241,268],"tested":[234,269],"on":[235],"numerous":[236],"lines":[238],"shown":[242],"inhibited":[249],"by":[250],"lactacystin":[251],"epoximicin.":[253],"designed":[258],"simple":[261],"\u201cadd":[262],"read\u201d":[264],"format":[265,280,299],"96":[271],"384-well":[273],"formats.":[274],"protease":[278,304],"was":[281],"at":[282],"least":[283],"10-fold":[284],"sensitive":[286,301],"than":[287],"comparable":[291],"several":[294],"proteases.":[295],"bioluminescent,":[297],"coupled-enzyme":[298],"rapid":[303],"ideal":[306],"high-throughput":[308],"screening.":[309]},"counts_by_year":[],"updated_date":"2026-09-29T14:09:16.559479","created_date":"2016-06-24T00:00:00"}