{"id":"https://openalex.org/W962912906","doi":null,"title":"\u51cb\u4ea1\u76f8\u5173\u86cb\u767d\uff08eIF\u20145A\uff09\u2014\u2014\u53cc\u5411\u7535\u6cf3\u5206\u79bb\u3001\u7f16\u7801\u57fa\u56e0\u514b\u9686\u548c\u8868\u8fbe\u3001\u591a\u6297\u5236\u5907","display_name":"\u51cb\u4ea1\u76f8\u5173\u86cb\u767d\uff08eIF\u20145A\uff09\u2014\u2014\u53cc\u5411\u7535\u6cf3\u5206\u79bb\u3001\u7f16\u7801\u57fa\u56e0\u514b\u9686\u548c\u8868\u8fbe\u3001\u591a\u6297\u5236\u5907","publication_year":2003,"publication_date":"2003-01-01","ids":{"openalex":"https://openalex.org/W962912906","mag":"962912906"},"language":"zh","primary_location":{"id":"mag:962912906","doi":null,"is_oa":false,"landing_page_url":"http://www.cqvip.com/QK/94263A/200306/9072876.html","pdf_url":null,"source":{"id":"https://openalex.org/S4306556889","display_name":"\u7ec6\u80de\u4e0e\u5206\u5b50\u514d\u75ab\u5b66\u6742\u5fd7","issn_l":"1007-8738","issn":["1007-8738"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":["medline","poland-20"],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"\u7ec6\u80de\u4e0e\u5206\u5b50\u514d\u75ab\u5b66\u6742\u5fd7","raw_type":null},"volume":null,"type":"article","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5035795618","display_name":"\u80e1\u7f8e\u8339","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"\u80e1\u7f8e\u8339","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5013829149","display_name":"\u9773\u5b9d\u950b","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"\u9773\u5b9d\u5cf0","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5042755951","display_name":"\u4f55\u6606","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"\u4f55\u6606","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5071237147","display_name":"\u5f20\u5b66\u654f","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"\u5f20\u5b66\u654f","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5084063243","display_name":"\u6c88\u500d\u594b","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"\u6c88\u500d\u594b","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.06551519,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"19","issue":"6","first_page":"573","last_page":"575"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10519","display_name":"Advanced Proteomics Techniques and Applications","score":0.9663000106811523,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T10519","display_name":"Advanced Proteomics Techniques and Applications","score":0.9663000106811523,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10294","display_name":"Cell death mechanisms and regulation","score":0.010499999858438969,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11041","display_name":"Ubiquitin and proteasome pathways","score":0.0035000001080334187,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/eif5a","display_name":"eIF5A","score":0.9959999918937683},{"id":"https://openalex.org/keywords/apoptosis-related-proteins","display_name":"apoptosis-related proteins","score":0.8989999890327454},{"id":"https://openalex.org/keywords/maldi-tof-mass-spectrometry","display_name":"MALDI-TOF mass spectrometry","score":0.9610000252723694},{"id":"https://openalex.org/keywords/esi-ms-ms","display_name":"electrospray ionization tandem mass spectrometry","score":0.9319999814033508},{"id":"https://openalex.org/keywords/gene-cloning","display_name":"gene cloning","score":0.7020000219345093},{"id":"https://openalex.org/keywords/recombinant-protein-expression","display_name":"recombinant protein expression","score":0.8709999918937683},{"id":"https://openalex.org/keywords/polyclonal-antibody-preparation","display_name":"polyclonal antibody preparation","score":0.9129999876022339}],"concepts":[{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.39421987533569336}],"mesh":[],"locations_count":1,"locations":[{"id":"mag:962912906","doi":null,"is_oa":false,"landing_page_url":"http://www.cqvip.com/QK/94263A/200306/9072876.html","pdf_url":null,"source":{"id":"https://openalex.org/S4306556889","display_name":"\u7ec6\u80de\u4e0e\u5206\u5b50\u514d\u75ab\u5b66\u6742\u5fd7","issn_l":"1007-8738","issn":["1007-8738"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":["medline","poland-20"],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"\u7ec6\u80de\u4e0e\u5206\u5b50\u514d\u75ab\u5b66\u6742\u5fd7","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.504800021648407}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W921956899","https://openalex.org/W3031445360","https://openalex.org/W1016073883","https://openalex.org/W3032682357","https://openalex.org/W4251055719","https://openalex.org/W2261394329","https://openalex.org/W1570489843","https://openalex.org/W4230617580","https://openalex.org/W3028994980","https://openalex.org/W7016561207"],"abstract_inverted_index":{"\u86cb\u767d\u8d28\u7ec4\u662f\u6307\u7279\u5b9a\u7ec6\u80de\u3001\u7ec4\u7ec7\u6216\u751f\u7269\u4f53\u5728\u7279\u5b9a\u65f6\u95f4\u6240\u8868\u8fbe\u7684\u5168\u90e8\u86cb\u767d,":[0],"\u5bf9\u5176\u8fdb\u884c\u5206\u6790\u7684\u5b66\u79d1\u88ab\u79f0\u4e3a\u86cb\u767d\u8d28\u7ec4\u5b66[1].\u5728\u86cb\u767d\u8d28\u7ec4\u5b66\u7684\u8bf8\u591a\u7814\u7a76\u65b9\u6cd5\u4e2d,":[1],"\u51fa\u73b0\u6700\u65e9\u4e14\u76ee\u524d\u4ecd\u80fd\u63d0\u4f9b\u86cb\u767d\u8d28\u7ec4\u5206\u6790\u6240\u8981\u6c42\u7684\u9ad8\u5206\u8fa8\u7387\u7684\u552f\u4e00\u6280\u672f,":[2],"\u662f\u53cc\u5411\u7535\u6cf3(2-D":[3],"PAGE),":[4],"\u7531\u4e8e\u5176\u53ef\u63d0\u9ad8\u5206\u8fa8\u7387\u3001\u91cd\u590d\u6027\u548c\u7b80\u6613\u6027\u826f\u597d,":[5],"\u5df2\u6210\u4e3a\u86cb\u767d\u8d28\u7ec4\u7814\u7a76\u7684\u6838\u5fc3\u6280\u672f[2].2-D":[6],"PAGE\u7528\u4e8e\u86cb\u767d\u8d28\u7ec4\u86cb\u767d\u8868\u8fbe\u7684\u5168\u9762\u5206\u6790\u65f6,":[7],"\u80fd\u591f\u5206\u79bb\u7279\u5b9a\u7ec6\u80de\u548c\u7ec4\u7ec7\u4e2d\u6210\u767e\u4e0a\u5343\u7684\u86cb\u767d\u5f62\u5f0f(\u5305\u62ec\u7ffb\u8bd1\u540e\u7684\u53d8\u5f02\u4f53).\u672c\u7814\u7a76\u4e2d,":[8],"\u6211\u4eec\u4ee5\u767d\u8840\u75c5\u7ec6\u80de\u7cfb(Mo-7e\u7ec6\u80de)\u4e3a\u4f8b,":[9],"\u91c7\u75282-D":[10],"PAGE\u6280\u672f\u5206\u79bb\u51cb\u4ea1\u76f8\u5173\u86cb\u767d,":[11],"\u7528\u4e24\u79cd\u5e38\u7528\u7684\u8d28\u8c31\u65b9\u6cd5MALDI-TOF/MS\u548cESI/MS/MS,":[12],"\u5bf9\u5176\u4e2d\u7684T\u70b9\u8fdb\u884c\u4e86\u9274\u5b9a.\u4ee5\u8bbe\u8ba1\u7684\u5f15\u7269,":[13],"\u91c7\u7528RT-PCR\u4ece\u57fa\u56e0\u7ec4\u4e2d\u9493\u53d6\u4e86\u8be5\u57fa\u56e0(eIF-5A),":[14],"\u5e76\u5728E.coli\u4e2d\u83b7\u5f97\u8868\u8fbe.\u7eaf\u5316\u7684\u91cd\u7ec4\u86cb\u767d\u514d\u75ab\u5bb6\u5154\u83b7\u5f97\u4e86\u6297eIF-5A\u7684\u591a\u6297,":[15],"\u4e3a\u540e\u7eed\u7684eIF-5A\u529f\u80fd\u7814\u7a76\u63d0\u4f9b\u4e86\u68c0\u6d4b\u5de5\u5177.\u5728\u5e94\u7528\u5bbdpH\u8303\u56f4\u80f6\u6761(pH":[16],"3\uff5e10)\u5206\u79bb\u767d\u8840\u75c5\u7ec6\u80de\u51cb\u4ea1\u76f8\u5173\u86cb\u767d\u7684\u57fa\u7840\u4e0a,":[17],"\u8fdb\u4e00\u6b65\u91c7\u7528\u7a84pH\u8303\u56f4\u80f6\u6761(pH":[18],"4\uff5e7)\u5206\u79bb\u51cb\u4ea1\u76f8\u5173\u86cb\u767d,":[19],"\u4f7f\u5206\u79bb\u548c\u8bc6\u522b\u5dee\u5f02\u86cb\u767d\u7684\u6548\u679c\u660e\u663e\u5f97\u5230\u6539\u5584,":[20],"\u4e14\u80fd\u591f\u8bc6\u522b\u76f8\u540c\u533a\u57df\u4e2d\u66f4\u591a\u7684\u5dee\u5f02\u86cb\u767d.":[21]},"counts_by_year":[],"updated_date":"2026-09-25T08:18:29.962427","created_date":"2016-06-24T00:00:00"}