{"id":"https://openalex.org/W9544219","doi":null,"title":"Improved 2D nano-LC/MS for proteomics applications: a comparative analysis using yeast proteome.","display_name":"Improved 2D nano-LC/MS for proteomics applications: a comparative analysis using yeast proteome.","publication_year":2004,"publication_date":"2004-06-01","ids":{"openalex":"https://openalex.org/W9544219","mag":"9544219","pmid":"https://pubmed.ncbi.nlm.nih.gov/15190086","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/2291680"},"language":"en","primary_location":{"id":"pmh:oai:pubmedcentral.nih.gov:2291680","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2291680","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"J Biomol Tech","raw_type":"Text"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2291680","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5003608562","display_name":"Edgar N\u00e4gele","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210129668","display_name":"Agilent Technologies (Germany)","ror":"https://ror.org/0355p2e87","country_code":"DE","type":"company","lineage":["https://openalex.org/I138285227","https://openalex.org/I4210129668"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"E N\u00e4gele","raw_affiliation_strings":["Agilent Technologies, 76337 Walbronn, Germany. edgar_naegele@agilent.com"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Agilent Technologies, 76337 Walbronn, Germany. edgar_naegele@agilent.com","institution_ids":["https://openalex.org/I4210129668"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111938529","display_name":"Martin Dominik Vollmer","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"M Vollmer","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5040078682","display_name":"Patric H\u00f6rth","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"P H\u00f6rth","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.5405,"has_fulltext":false,"cited_by_count":33,"citation_normalized_percentile":{"value":0.54076632,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":87,"max":98},"biblio":{"volume":"15","issue":"2","first_page":"134","last_page":"43"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10519","display_name":"Advanced Proteomics Techniques and Applications","score":0.9951000213623047,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T10519","display_name":"Advanced Proteomics Techniques and Applications","score":0.9951000213623047,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10683","display_name":"Mass Spectrometry Techniques and Applications","score":0.0035000001080334187,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10908","display_name":"Analytical Chemistry and Chromatography","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1602","display_name":"Analytical Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/two-dimensional-liquid-chromatography","display_name":"two-dimensional liquid chromatography","score":0.7670000195503235},{"id":"https://openalex.org/keywords/strong-cation-exchange-chromatography","display_name":"strong cation exchange chromatography","score":0.9399999976158142},{"id":"https://openalex.org/keywords/salt-injection","display_name":"salt injection","score":0.3840000033378601},{"id":"https://openalex.org/keywords/protein-identification","display_name":"protein identification","score":0.6290000081062317},{"id":"https://openalex.org/keywords/yeast-proteome","display_name":"yeast proteome","score":0.9589999914169312},{"id":"https://openalex.org/keywords/tryptic-digest","display_name":"tryptic digest","score":0.8640000224113464},{"id":"https://openalex.org/keywords/peptide-separation","display_name":"peptide separation","score":0.414000004529953}],"concepts":[{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.8084720373153687},{"id":"https://openalex.org/C104397665","wikidata":"https://www.wikidata.org/wiki/Q860947","display_name":"Proteome","level":2,"score":0.7727630138397217},{"id":"https://openalex.org/C2776371256","wikidata":"https://www.wikidata.org/wiki/Q12370","display_name":"Salt (chemistry)","level":2,"score":0.6963270902633667},{"id":"https://openalex.org/C46111723","wikidata":"https://www.wikidata.org/wiki/Q471857","display_name":"Proteomics","level":3,"score":0.6289078593254089},{"id":"https://openalex.org/C162356407","wikidata":"https://www.wikidata.org/wiki/Q180809","display_name":"Mass spectrometry","level":2,"score":0.5354084968566895},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5252211093902588},{"id":"https://openalex.org/C73826308","wikidata":"https://www.wikidata.org/wiki/Q684907","display_name":"Elution","level":2,"score":0.5250972509384155},{"id":"https://openalex.org/C206967267","wikidata":"https://www.wikidata.org/wiki/Q905749","display_name":"Ion chromatography","level":2,"score":0.48746347427368164},{"id":"https://openalex.org/C3017686574","wikidata":"https://www.wikidata.org/wiki/Q381233","display_name":"Gradient elution","level":3,"score":0.42490899562835693},{"id":"https://openalex.org/C179998833","wikidata":"https://www.wikidata.org/wiki/Q381233","display_name":"High-performance liquid chromatography","level":2,"score":0.35617128014564514},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.11313164234161377},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0},{"id":"https://openalex.org/C147789679","wikidata":"https://www.wikidata.org/wiki/Q11372","display_name":"Physical chemistry","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D002852","descriptor_name":"Chromatography, Ion Exchange","qualifier_ui":"Q000295","qualifier_name":"instrumentation","is_major_topic":false},{"descriptor_ui":"D002852","descriptor_name":"Chromatography, Ion Exchange","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D007526","descriptor_name":"Isoelectric Point","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008970","descriptor_name":"Molecular Weight","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010446","descriptor_name":"Peptide Fragments","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D012441","descriptor_name":"Saccharomyces cerevisiae","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D020543","descriptor_name":"Proteome","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D021241","descriptor_name":"Spectrometry, Mass, Electrospray Ionization","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D029701","descriptor_name":"Saccharomyces cerevisiae Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D029701","descriptor_name":"Saccharomyces cerevisiae Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D030562","descriptor_name":"Databases, Protein","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D036103","descriptor_name":"Nanotechnology","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D040901","descriptor_name":"Proteomics","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true}],"locations_count":4,"locations":[{"id":"pmh:oai:pubmedcentral.nih.gov:2291680","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2291680","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"J Biomol Tech","raw_type":"Text"},{"id":"pmid:15190086","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/15190086","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of biomolecular techniques : JBT","raw_type":"Comparative Study"},{"id":"mag:2476682372","doi":null,"is_oa":false,"landing_page_url":"https://www.researchgate.net/profile/Edgar_Naegele/publication/8517408_Improved_2D_nano-LCMS_for_proteomics_applications_a_comparative_analysis_using_yeast_proteome/links/00b7d52b173ace9387000000.pdf","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null},{"id":"mag:9544219","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/15190086","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":{"id":"pmh:oai:pubmedcentral.nih.gov:2291680","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2291680","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"J Biomol Tech","raw_type":"Text"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":5,"referenced_works":["https://openalex.org/W134678827","https://openalex.org/W1597678601","https://openalex.org/W1984369447","https://openalex.org/W1987531784","https://openalex.org/W2156022009"],"related_works":["https://openalex.org/W2074749328","https://openalex.org/W7095475966","https://openalex.org/W2380535609","https://openalex.org/W893359406","https://openalex.org/W2395447150","https://openalex.org/W7097311957","https://openalex.org/W2476682372","https://openalex.org/W2740267557","https://openalex.org/W42579230","https://openalex.org/W2051797601"],"abstract_inverted_index":{"The":[0,104,139],"most":[1],"commonly":[2,148],"used":[3,149],"method":[4,85,108,132,143,151],"for":[5,86],"protein":[6],"identification":[7],"with":[8,97,146,154],"two-dimensional":[9],"(2D)":[10],"online":[11,88],"liquid":[12],"chromatography-mass":[13],"spectrometry":[14],"(LC/MS)":[15],"involves":[16],"the":[17,59,69,74,92,112,124,128,134,147],"elution":[18],"of":[19,34,62,76,106,114,118,123],"digest":[20,122],"peptides":[21],"from":[22],"a":[23,83,115,119],"strong":[24,77],"cation":[25,78],"exchange":[26,48,79],"column":[27],"by":[28,39],"an":[29,98,155],"injected":[30,60,93,129],"salt":[31,36,63,94,102,130,137,142],"step":[32,95],"gradient":[33,96],"increasing":[35],"concentration":[37],"followed":[38],"reversed":[40],"phase":[41],"separation.":[42],"However,":[43],"in":[44,111],"this":[45,107],"approach":[46],"ion":[47],"chromatography":[49],"does":[50],"not":[51,66],"perform":[52],"to":[53,68],"its":[54],"fullest":[55],"extent,":[56],"primarily":[57],"because":[58],"volume":[61],"solution":[64,131],"is":[65,109],"optimized":[67,99],"SCX":[70],"column.":[71],"To":[72],"improve":[73],"performance":[75],"chromatography,":[80],"we":[81],"developed":[82],"new":[84],"2D":[87],"nano-LC/MS":[89],"that":[90],"replaces":[91],"semicontinuous":[100,135,140],"pumped":[101],"gradient.":[103],"viability":[105],"demonstrated":[110],"results":[113],"comparative":[116],"analysis":[117],"complex":[120],"tryptic":[121],"yeast":[125],"proteome":[126],"using":[127],"and":[133,152],"pump":[136,141],"method.":[138,158],"compares":[144],"favorably":[145],"injection":[150],"also":[153],"offline":[156],"2D-LC":[157]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":1},{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":2},{"year":2017,"cited_by_count":2},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":6},{"year":2014,"cited_by_count":2},{"year":2012,"cited_by_count":4}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}