{"id":"https://openalex.org/W950475583","doi":null,"title":"Effect of lidamycin on telomerase activity in human hepatoma BEL-7402 cells.","display_name":"Effect of lidamycin on telomerase activity in human hepatoma BEL-7402 cells.","publication_year":2007,"publication_date":"2007-06-01","ids":{"openalex":"https://openalex.org/W950475583","mag":"950475583","pmid":"https://pubmed.ncbi.nlm.nih.gov/17672208"},"language":"en","primary_location":{"id":"pmid:17672208","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/17672208","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biomedical and environmental sciences : BES","raw_type":"Journal Article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5108260645","display_name":"Ruijuan Gao","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I200296433","display_name":"Chinese Academy of Medical Sciences & Peking Union Medical College","ror":"https://ror.org/02drdmm93","country_code":"CN","type":"education","lineage":["https://openalex.org/I200296433"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Rui-Juan Gao","raw_affiliation_strings":["Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China","institution_ids":["https://openalex.org/I200296433"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5002128593","display_name":"Yue-Xin Liang","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Yue-Xin Liang","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5048914862","display_name":"Dian-Dong Li","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Dian-Dong Li","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5047348728","display_name":"Hongyin Zhang","orcid":"https://orcid.org/0000-0001-6906-4713","observed_orcids":["https://orcid.org/0000-0001-6906-4713","https://orcid.org/0000-0001-5405-8097"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Hong-Yin Zhang","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5110364299","display_name":"Yong\u2010Su Zhen","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Yong-Su Zhen","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":11,"citation_normalized_percentile":{"value":0.06497541,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":96},"biblio":{"volume":"20","issue":"3","first_page":"189","last_page":"97"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10937","display_name":"Telomeres, Telomerase, and Senescence","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10937","display_name":"Telomeres, Telomerase, and Senescence","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10294","display_name":"Cell death mechanisms and regulation","score":0.00039999998989515007,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10583","display_name":"Cancer-related Molecular Pathways","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/lidamycin","display_name":"lidamycin","score":0.9980000257492065},{"id":"https://openalex.org/keywords/telomerase-activity","display_name":"telomerase activity","score":0.9710000157356262},{"id":"https://openalex.org/keywords/hepatocellular-carcinoma","display_name":"hepatocellular carcinoma","score":0.7889999747276306},{"id":"https://openalex.org/keywords/bel-7402-cells","display_name":"BEL-7402 cells","score":0.9810000061988831},{"id":"https://openalex.org/keywords/mitotic-cell-death","display_name":"mitotic cell death","score":0.9950000047683716},{"id":"https://openalex.org/keywords/cellular-senescence","display_name":"cellular senescence","score":0.9490000009536743},{"id":"https://openalex.org/keywords/multinucleation","display_name":"multinucleation","score":0.7049999833106995},{"id":"https://openalex.org/keywords/chromatin-condensation","display_name":"chromatin condensation","score":0.906000018119812},{"id":"https://openalex.org/keywords/mitochondrial-membrane-potential","display_name":"mitochondrial membrane potential","score":0.9929999709129333},{"id":"https://openalex.org/keywords/antitumor-antibiotics","display_name":"antitumor antibiotics","score":0.41499999165534973}],"concepts":[{"id":"https://openalex.org/C94581717","wikidata":"https://www.wikidata.org/wiki/Q331839","display_name":"Telomerase","level":3,"score":0.9201771020889282},{"id":"https://openalex.org/C93304396","wikidata":"https://www.wikidata.org/wiki/Q14763008","display_name":"Mitosis","level":2,"score":0.6981118321418762},{"id":"https://openalex.org/C522857546","wikidata":"https://www.wikidata.org/wiki/Q2070979","display_name":"Senescence","level":2,"score":0.6341404914855957},{"id":"https://openalex.org/C177336024","wikidata":"https://www.wikidata.org/wiki/Q14872779","display_name":"Telomere","level":3,"score":0.6141689419746399},{"id":"https://openalex.org/C190283241","wikidata":"https://www.wikidata.org/wiki/Q14599311","display_name":"Apoptosis","level":2,"score":0.5703402161598206},{"id":"https://openalex.org/C2778271344","wikidata":"https://www.wikidata.org/wiki/Q903395","display_name":"Agarose gel electrophoresis","level":3,"score":0.5611624121665955},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.5522496700286865},{"id":"https://openalex.org/C186000732","wikidata":"https://www.wikidata.org/wiki/Q6881914","display_name":"Mitotic catastrophe","level":4,"score":0.5241420865058899},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5062114596366882},{"id":"https://openalex.org/C31573885","wikidata":"https://www.wikidata.org/wiki/Q304484","display_name":"Programmed cell death","level":3,"score":0.4928715229034424},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.4859505891799927},{"id":"https://openalex.org/C125593758","wikidata":"https://www.wikidata.org/wiki/Q15315135","display_name":"Telomerase reverse transcriptase","level":4,"score":0.46329036355018616},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.434209406375885},{"id":"https://openalex.org/C74864618","wikidata":"https://www.wikidata.org/wiki/Q2332446","display_name":"Staining","level":2,"score":0.43175217509269714},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.32203608751296997},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.21071869134902954},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.1766917109489441},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.11654871702194214},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.07394799590110779}],"mesh":[{"descriptor_ui":"D000617","descriptor_name":"Aminoglycosides","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true},{"descriptor_ui":"D000903","descriptor_name":"Antibiotics, Antineoplastic","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true},{"descriptor_ui":"D001399","descriptor_name":"Azure Stains","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001562","descriptor_name":"Benzimidazoles","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001616","descriptor_name":"beta-Galactosidase","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D002467","descriptor_name":"Cell Nucleus","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D002467","descriptor_name":"Cell Nucleus","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D002843","descriptor_name":"Chromatin","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D004273","descriptor_name":"DNA, Neoplasm","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D004305","descriptor_name":"Dose-Response Relationship, Drug","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006528","descriptor_name":"Carcinoma, Hepatocellular","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D006528","descriptor_name":"Carcinoma, Hepatocellular","qualifier_ui":"Q000473","qualifier_name":"pathology","is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008113","descriptor_name":"Liver Neoplasms","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D008113","descriptor_name":"Liver Neoplasms","qualifier_ui":"Q000473","qualifier_name":"pathology","is_major_topic":true},{"descriptor_ui":"D008938","descriptor_name":"Mitosis","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D010641","descriptor_name":"Phenotype","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011419","descriptor_name":"Propidium","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013997","descriptor_name":"Time Factors","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015894","descriptor_name":"Genome, Human","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D016922","descriptor_name":"Cellular Senescence","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D017209","descriptor_name":"Apoptosis","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D019098","descriptor_name":"Telomerase","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D053078","descriptor_name":"Membrane Potential, Mitochondrial","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D053281","descriptor_name":"Enediynes","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true}],"locations_count":2,"locations":[{"id":"pmid:17672208","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/17672208","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biomedical and environmental sciences : BES","raw_type":"Journal Article"},{"id":"mag:3151110127","doi":null,"is_oa":false,"landing_page_url":"http://www.cqvip.com/QK/84046X/200703/25173550.html","pdf_url":null,"source":{"id":"https://openalex.org/S4306555121","display_name":"\u751f\u7269\u533b\u5b66\u4e0e\u73af\u5883\u79d1\u5b66\uff1a\u82f1\u6587\u7248","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"\u751f\u7269\u533b\u5b66\u4e0e\u73af\u5883\u79d1\u5b66\uff1a\u82f1\u6587\u7248","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.9391000270843506,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":38,"referenced_works":["https://openalex.org/W277933975","https://openalex.org/W1496772673","https://openalex.org/W1973527515","https://openalex.org/W1982310288","https://openalex.org/W1982763595","https://openalex.org/W1985392868","https://openalex.org/W1991563816","https://openalex.org/W1997463702","https://openalex.org/W2014361961","https://openalex.org/W2022328735","https://openalex.org/W2030976397","https://openalex.org/W2030998537","https://openalex.org/W2058838934","https://openalex.org/W2073429636","https://openalex.org/W2085968257","https://openalex.org/W2087404852","https://openalex.org/W2087849372","https://openalex.org/W2089519256","https://openalex.org/W2089663038","https://openalex.org/W2093837468","https://openalex.org/W2104666653","https://openalex.org/W2104908670","https://openalex.org/W2114551234","https://openalex.org/W2116813871","https://openalex.org/W2118228848","https://openalex.org/W2125547572","https://openalex.org/W2144402710","https://openalex.org/W2146108477","https://openalex.org/W2156849341","https://openalex.org/W2166732620","https://openalex.org/W2187421296","https://openalex.org/W2315283231","https://openalex.org/W2319134851","https://openalex.org/W2326648943","https://openalex.org/W2378878500","https://openalex.org/W2389562929","https://openalex.org/W2391365265","https://openalex.org/W2514765080"],"related_works":["https://openalex.org/W2383092134","https://openalex.org/W2376270691","https://openalex.org/W2085968257","https://openalex.org/W249980275","https://openalex.org/W2141405392","https://openalex.org/W2261781601","https://openalex.org/W2362200844","https://openalex.org/W1646808","https://openalex.org/W1995799097","https://openalex.org/W1476614251"],"abstract_inverted_index":{"OBJECTIVE:":[0],"To":[1],"investigate":[2],"the":[3,17,74,147,194],"effect":[4],"of":[5,19,57,79,139,149,177,202],"lidamycin":[6],"(LDM)":[7],"on":[8],"telomerase":[9,91,186],"activity":[10,87,145,154,187],"in":[11,98,158,185,193],"human":[12],"hepatoma":[13,160,178],"BEL-7402":[14,161,179],"cells":[15,100,180],"under":[16],"condition":[18],"LDM":[20],"inducing":[21],"mitotic":[22],"cell":[23,95,165],"death":[24,96,166],"and":[25,37,46,67,104,109,136,146,167,197],"senescence.":[26],"METHODS:":[27],"Chromatin":[28],"condensation":[29],"was":[30,41,49,59,81,88,132],"detected":[31],"by":[32,43,51,83,90,102,134],"co-staining":[33],"with":[34],"Hoechst":[35],"33342":[36],"PI.":[38],"Cell":[39],"multinucleation":[40,108],"observed":[42],"Giemsa":[44],"staining":[45,69],"genomic":[47],"DNA":[48,119],"separated":[50],"agarose":[52],"gel":[53],"electrophoresis.":[54],"Fluorescent":[55],"intensity":[56],"Rho123":[58],"determined":[60],"for":[61],"mitochondrial":[62,111],"membrane":[63,112],"potential.":[64,113],"MTT":[65],"assay":[66],"SA-beta-gal":[68,144],"were":[70,121],"employed":[71],"to":[72,181],"analyze":[73],"senescence-like":[75],"phenotype.":[76],"The":[77,183],"expression":[78],"proteins":[80,126],"analyzed":[82],"Western":[84],"blot.":[85],"Telomerase":[86,153],"assayed":[89],"PCR-ELISA.":[92],"RESULTS:":[93],"Mitotic":[94,164],"occurred":[97],"LDM-treated":[99,159],"characterized":[101],"unique":[103],"atypical":[105],"chromatin":[106],"condensation,":[107],"increased":[110,135],"However,":[114],"no":[115],"apoptotic":[116],"bodies":[117],"or":[118,173],"ladders":[120],"found.":[122],"In":[123],"addition,":[124],"apoptosis-related":[125],"remained":[127],"nearly":[128],"unaltered.":[129],"Senescence-like":[130],"phenotype":[131],"identified":[133],"elongated":[137],"size":[138],"cells,":[140],"growth":[141],"retardation,":[142],"enhanced":[143],"changes":[148],"senescence-related":[150],"protein":[151],"expression.":[152],"markedly":[155],"decreased":[156],"(P<0.01)":[157],"cells.":[162],"CONCLUSION:":[163],"senescence":[168],"could":[169],"be":[170],"triggered":[171],"simultaneously":[172],"sequentially":[174],"after":[175],"exposure":[176],"LDM.":[182],"decrease":[184],"may":[188],"play":[189],"a":[190],"key":[191],"role":[192],"defective":[195],"mitosis":[196],"aging":[198],"morphology.":[199],"Further":[200],"investigation":[201],"detailed":[203],"mechanism":[204],"is":[205],"needed.":[206]},"counts_by_year":[{"year":2024,"cited_by_count":1},{"year":2019,"cited_by_count":1},{"year":2016,"cited_by_count":2},{"year":2015,"cited_by_count":2},{"year":2013,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}