{"id":"https://openalex.org/W923729407","doi":"https://doi.org/10.5283/epub.12378","title":"Generierung von Killer-artifiziellen-antigenpr\u00e4sentierenden Zellen (KaAPC) zur antigenspezifischen Eliminierung von humanen prim\u00e4ren CD8+ zytotoxischen T-Lymphozyten in vitro","display_name":"Generierung von Killer-artifiziellen-antigenpr\u00e4sentierenden Zellen (KaAPC) zur antigenspezifischen Eliminierung von humanen prim\u00e4ren CD8+ zytotoxischen T-Lymphozyten in vitro","publication_year":2009,"publication_date":"2009-01-01","ids":{"openalex":"https://openalex.org/W923729407","doi":"https://doi.org/10.5283/epub.12378","mag":"923729407"},"language":"de","primary_location":{"id":"pmh:oai:epub.uni-regensburg.de:12378","doi":null,"is_oa":false,"landing_page_url":"https://epub.uni-regensburg.de/12378/1/Doktorarbeit_final.pdf","pdf_url":"https://epub.uni-regensburg.de/12378/1/Doktorarbeit_final.pdf","source":{"id":"https://openalex.org/S4306401229","display_name":"University of Regensburg Publication Server (University of Regensburg)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I60668342","host_organization_name":"University of Regensburg","host_organization_lineage":["https://openalex.org/I60668342"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"","raw_type":"thesis"},"parent_works":[],"lineage":["https://openalex.org/W923729407"],"child_works_count":0,"type":"dissertation","is_traditional":true,"indexed_in":["datacite"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://doi.org/10.5283/epub.12378","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5059054609","display_name":"Christian Sch\u00fctz","orcid":"https://orcid.org/0000-0002-2361-2785","observed_orcids":["https://orcid.org/0000-0002-2361-2785"]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Sch\u00fctz, Christian","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5059054609"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10580","display_name":"Immunotherapy and Immune Responses","score":0.8335000276565552,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10580","display_name":"Immunotherapy and Immune Responses","score":0.8335000276565552,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10031","display_name":"T-cell and B-cell Immunology","score":0.1509999930858612,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11016","display_name":"Monoclonal and Polyclonal Antibodies Research","score":0.0017999999690800905,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/cytotoxic-cd8-t-cells","display_name":"CD8+ cytotoxic T lymphocytes","score":0.9120000004768372},{"id":"https://openalex.org/keywords/fas-ligand","display_name":"Fas ligand","score":0.6909999847412109},{"id":"https://openalex.org/keywords/t-cell-apoptosis","display_name":"T cell apoptosis","score":0.6489999890327454},{"id":"https://openalex.org/keywords/melan-a","display_name":"Melan-A","score":0.5329999923706055},{"id":"https://openalex.org/keywords/cmv-pp65","display_name":"CMV pp65","score":0.9359999895095825},{"id":"https://openalex.org/keywords/t-cell-activation","display_name":"T cell activation","score":0.3659999966621399}],"concepts":[{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.7110299468040466},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.6102896928787231},{"id":"https://openalex.org/C167672396","wikidata":"https://www.wikidata.org/wiki/Q417800","display_name":"CD8","level":3,"score":0.46817106008529663},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.2463928759098053},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.21111026406288147},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.17495763301849365},{"id":"https://openalex.org/C147483822","wikidata":"https://www.wikidata.org/wiki/Q103537","display_name":"Antigen","level":2,"score":0.13096883893013}],"mesh":[],"locations_count":4,"locations":[{"id":"pmh:oai:epub.uni-regensburg.de:12378","doi":null,"is_oa":false,"landing_page_url":"https://epub.uni-regensburg.de/12378/1/Doktorarbeit_final.pdf","pdf_url":"https://epub.uni-regensburg.de/12378/1/Doktorarbeit_final.pdf","source":{"id":"https://openalex.org/S4306401229","display_name":"University of Regensburg Publication Server (University of Regensburg)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I60668342","host_organization_name":"University of Regensburg","host_organization_lineage":["https://openalex.org/I60668342"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"","raw_type":"thesis"},{"id":"pmh:oai:aleph.bib-bvb.de:BVB01-018761207","doi":null,"is_oa":false,"landing_page_url":"https://nbn-resolving.org/urn:nbn:de:bvb:355-opus-14105","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Hochschulschrift"},{"id":"doi:10.5283/epub.12378","doi":"https://doi.org/10.5283/epub.12378","is_oa":true,"landing_page_url":"https://doi.org/10.5283/epub.12378","pdf_url":null,"source":{"id":"https://openalex.org/S4306401229","display_name":"University of Regensburg Publication Server (University of Regensburg)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I60668342","host_organization_name":"University of Regensburg","host_organization_lineage":["https://openalex.org/I60668342"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"article-journal"},{"id":"mag:923729407","doi":null,"is_oa":false,"landing_page_url":"https://epub.uni-regensburg.de/12378/","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"doi:10.5283/epub.12378","doi":"https://doi.org/10.5283/epub.12378","is_oa":true,"landing_page_url":"https://doi.org/10.5283/epub.12378","pdf_url":null,"source":{"id":"https://openalex.org/S4306401229","display_name":"University of Regensburg Publication Server (University of Regensburg)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I60668342","host_organization_name":"University of Regensburg","host_organization_lineage":["https://openalex.org/I60668342"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"article-journal"},"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.9476000070571899}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2089116024","https://openalex.org/W2013325236","https://openalex.org/W7096973395","https://openalex.org/W2370661862","https://openalex.org/W1980250464","https://openalex.org/W2153961204","https://openalex.org/W2897446187","https://openalex.org/W2566323237","https://openalex.org/W1504394780","https://openalex.org/W2980101677"],"abstract_inverted_index":{"In":[0],"der":[1,35,38,40,64,117,156,283,302,331,373,411,441,444,458],"vorliegenden":[2],"Arbeit":[3,400],"wurde":[4,97,307,327],"eine":[5,425],"\ufffdbead\ufffd-basierte":[6],"Killer-artifizielle-antigenpr\u00e4sentierende":[7],"Zelle":[8],"(KaAPC)":[9],"generiert,":[10],"die":[11,115,154,165,230,238,255,270,298,353,367,454],"mittels":[12],"des":[13,18],"gebundenen":[14],"HLA-A2-Ig":[15,57,88,243],"Dimers":[16],"und":[17,37,60,91,100,175,216,289,335,337,344],"anti-Fas":[19,61,94,249],"Antik\u00f6rpers,":[20],"zur":[21,76,159,329,429,446],"antigenspezifischen":[22,27,77,109,121,189,217,272,286],"Ausschaltung":[23,39],"von":[24,66,79,214,274,282,341,358,431,448],"pathogenetisch":[25],"relevanten":[26],"CD8+":[28,46,80,110,124,142,176,190,199,218,275,312,380,389,419],"T-Zellen":[29,81,125,143,191,200,219,276,313,319,381,390,420],"bef\u00e4higt":[30],"ist.":[31],"Nach":[32],"anf\u00e4nglicher":[33],"Optimierung":[34],"Kulturbedingungen":[36],"Effekte":[41],"freien":[42],"Peptids":[43],"auf":[44,63,229],"antigenspezifische":[45,166,311,379],"T-Zellkokulturen,":[47],"wurden":[48,53],"verschiedene":[49],"KaAPC-Ph\u00e4notypen":[50],"hergestellt.":[51],"Hierzu":[52,326],"unterschiedliche":[54],"Mengen":[55,86,241],"an":[56,87,242],"Dimer":[58,89,244],"Molek\u00fclen":[59,90],"Antik\u00f6rpern":[62],"Oberfl\u00e4che":[65],"paramagnetischen":[67],"Epoxybeads":[68],"immobilisiert.":[69],"Die":[70],"anschlie\u00dfende":[71],"Evaluierung":[72],"ergab":[73],"einen":[74],"optimalen,":[75],"Eliminierung":[78],"bef\u00e4higten,":[82],"KaAPC-Ph\u00e4notyp":[83,408],"mit":[84,128,138,194,211,236,239,320,391],"definierten":[85],"gleichzeitig":[92],"gebundenem":[93],"Antik\u00f6rper.":[95],"Dieser":[96,351],"durchflusszytometrisch":[98],"charakterisiert":[99],"sowohl":[101,277,402],"im":[102,106],"Mart-1":[103],"als":[104,280,404],"auch":[105,281,314,382,405],"CMVpp65":[107],"humanen,":[108],"T-Zellmodellsystem":[111],"funktionell":[112,406],"untersucht.":[113],"Durch":[114,366],"Bestimmung":[116],"Apoptoseinduktion":[118],"in":[119,187,258,360,398,443,455],"humanen":[120],"HLA-A2":[122],"restringierten":[123],"nach":[126,169,192,209],"Kokultur":[127,193],"unterschiedlich":[129],"beladenen":[130],"KaAPC":[131,157,174,195,215,232,303,378,418,459],"konnte":[132,181,372,409],"gezeigt":[133],"werden,":[134,376],"dass":[135,153,164,269,377],"KaAPC,":[136],"beladen":[137],"dem":[139],"korrespondierenden":[140],"Peptid,":[141],"antigenspezifisch":[144,421],"depletieren":[145,323],"k\u00f6nnen.":[146,395],"Dar\u00fcber":[147],"hinaus":[148],"ergaben":[149],"weitere":[150],"funktionelle":[151],"Experimente,":[152],"Effizienz":[155],"proportional":[158],"eingesetzten":[160],"Menge":[161],"war":[162],"und,":[163],"Depletion":[167,273],"schon":[168],"30":[170],"min":[171],"Kontaktzeit":[172],"zwischen":[173],"T-Zelle":[177],"ausgel\u00f6st":[178],"wurde.":[179],"Ebenso":[180],"kein":[182],"klassischer,":[183],"aktivierungsinduzierter":[184],"Zelltod":[185],"(AICD)":[186],"den":[188,257,259,397],"nachgewiesen":[196],"werden.":[197,416,462],"Antigenspezifische":[198],"exprimierten":[201],"weder":[202],"CD107a":[203],"noch":[204],"typische":[205],"Effektorzytokine":[206],"wie":[207],"TNF-alpha":[208],"Kontakt":[210],"KaAPC.":[212],"Kokulturen":[213,235],"unter":[220,328,338],"Zugabe":[221],"eines":[222,285,290],"neutralisierenden":[223],"anti-TNF-alpha":[224],"Antik\u00f6rpers":[225],"zeigten":[226,252,267],"keinerlei":[227],"Auswirkung":[228],"durch":[231],"hervorgerufene":[233],"Apoptoseinduktion.":[234],"Kontrollbeads,":[237],"gleichen":[240],"beschichtet":[245],"waren,":[246],"jedoch":[247],"keinen":[248],"Antik\u00f6rper":[250],"besa\u00dfen,":[251],"keine":[253],"Apoptoseinduktion,":[254],"\u00fcber":[256],"unstimulierten":[260],"T-Zellkulturen":[261],"gemessenen":[262],"Hintergrund":[263],"hinausging.":[264],"Diese":[265],"Ergebnisse":[266],"deutlich,":[268],"erfolgreiche":[271],"vom":[278],"Vorhandensein,":[279],"St\u00e4rke":[284],"(HLA-A2-Ig":[287],"Dimer)":[288],"Apoptose":[291],"induzierenden":[292],"Signals":[293],"(anti-Fas":[294],"Antik\u00f6rper)":[295],"abh\u00e4ngt.":[296],"Um":[297],"potentielle":[299],"therapeutische":[300],"Anwendbarkeit":[301],"weiter":[304],"zu":[305],"untermauern,":[306],"untersucht,":[308],"ob":[309],"diese":[310,424],"aus":[315,383],"einem":[316,384,438],"Gemisch":[317,386],"unterschiedlicher":[318],"verschiedenen":[321],"Antigenspezifit\u00e4ten":[322,393],"werden":[324],"konnten.":[325],"Hilfenahme":[330],"beiden":[332],"Membranfarbstoffe":[333],"PKH67":[334],"PKH26":[336],"gleichzeitiger":[339],"Verwendung":[340],"Annexin":[342],"V":[343],"7-ActinomycinD":[345],"ein":[346],"neuer":[347],"durchflusszytometrischer":[348],"Test":[349],"entwickelt.":[350],"erlaubte":[352],"gleichzeitige,":[354],"voneinander":[355],"getrennte":[356],"Evaluation":[357],"Apoptoseraten":[359],"unterschiedlichen":[361,392],"T-Zellpopulationen":[362],"innerhalb":[363],"desselben":[364],"Ansatzes.":[365],"Anwendung":[368],"dieser":[369,399],"neuen":[370],"Methode":[371],"Beweis":[374],"erbracht":[375,415],"heterogenen":[385],"autologer":[387],"Fas+":[388],"eliminieren":[394],"F\u00fcr":[396],"entwickelten,":[401],"ph\u00e4notypisch,":[403],"charakterisierten":[407],"somit":[410],"\ufffdproof":[412],"of":[413],"concept\ufffd":[414],"Da":[417],"eliminieren,":[422],"stellen":[423],"neue,":[426],"vielversprechende":[427],"M\u00f6glichkeit":[428],"Modulation":[430],"T-Zellvermittelten":[432],"Immunantworten":[433],"da.":[434],"Jedoch":[435],"m\u00fcssten":[436],"vor":[437],"potentiellen":[439],"Einsatz":[440],"KaAPC-Technologie":[442],"Klinik":[445],"Behandlung":[447],"Autoimmunerkrankungen,":[449],"Transplantatabsto\u00dfungsreaktionen":[450],"oder":[451],"chronischen":[452],"Infekten":[453],"vivo":[456],"Eigenschaften":[457],"sorgf\u00e4ltig":[460],"evaluiert":[461]},"counts_by_year":[],"updated_date":"2026-11-14T11:04:58.165337","created_date":"2016-06-24T00:00:00"}