{"id":"https://openalex.org/W91937263","doi":null,"title":"High density CHO cell cultures: Improved productivity and product quality","display_name":"High density CHO cell cultures: Improved productivity and product quality","publication_year":2014,"publication_date":"2014-03-01","ids":{"openalex":"https://openalex.org/W91937263","mag":"91937263"},"language":"en","primary_location":{"id":"pmh:http://www.rian.ie/86759/","doi":null,"is_oa":true,"landing_page_url":"http://doras.dcu.ie/19727/","pdf_url":"http://doras.dcu.ie/19727/","source":{"id":"https://openalex.org/S4306400033","display_name":"Arrow@dit (Dublin Institute of Technology)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4210144925","host_organization_name":"Technological University Dublin","host_organization_lineage":["https://openalex.org/I4210144925"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Byrne, Karen (2014) High density CHO cell cultures: Improved productivity and product quality. PhD thesis, Dublin City University.","raw_type":"Other"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"http://doras.dcu.ie/19727/","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":null,"display_name":"Karen M. Byrne","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Karen M. Byrne","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":1,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11663","display_name":"Viral Infectious Diseases and Gene Expression in Insects","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11663","display_name":"Viral Infectious Diseases and Gene Expression in Insects","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.0003000000142492354,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11016","display_name":"Monoclonal and Polyclonal Antibodies Research","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/cho-cell-culture","display_name":"CHO cell culture","score":0.8040000200271606},{"id":"https://openalex.org/keywords/high-cell-density-culture","display_name":"high cell density culture","score":0.671999990940094},{"id":"https://openalex.org/keywords/perfusion-culture","display_name":"perfusion culture","score":0.7710000276565552},{"id":"https://openalex.org/keywords/critical-process-parameters","display_name":"critical process parameters","score":0.7599999904632568},{"id":"https://openalex.org/keywords/glutamine","display_name":"glutamine","score":0.8519999980926514},{"id":"https://openalex.org/keywords/ammonia","display_name":"ammonia","score":0.5519999861717224},{"id":"https://openalex.org/keywords/protein-aggregation","display_name":"protein aggregation","score":0.8500000238418579},{"id":"https://openalex.org/keywords/product-quality","display_name":"product quality","score":0.5640000104904175},{"id":"https://openalex.org/keywords/cell-viability","display_name":"cell viability","score":0.6010000109672546},{"id":"https://openalex.org/keywords/biopharmaceutical-manufacturing","display_name":"biopharmaceutical manufacturing","score":0.6959999799728394},{"id":"https://openalex.org/keywords/process-analytical-technology","display_name":"process analytical technology","score":0.6679999828338623},{"id":"https://openalex.org/keywords/cell-yield","display_name":"cell yield","score":0.3269999921321869}],"concepts":[{"id":"https://openalex.org/C4916135","wikidata":"https://www.wikidata.org/wiki/Q4119055","display_name":"Bioprocess","level":2,"score":0.7427899241447449},{"id":"https://openalex.org/C2781047461","wikidata":"https://www.wikidata.org/wiki/Q679692","display_name":"Biopharmaceutical","level":2,"score":0.6879822015762329},{"id":"https://openalex.org/C2779349466","wikidata":"https://www.wikidata.org/wiki/Q181619","display_name":"Glutamine","level":3,"score":0.5569030046463013},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.5149949193000793},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.5032116770744324},{"id":"https://openalex.org/C178144697","wikidata":"https://www.wikidata.org/wiki/Q2111609","display_name":"Process analytical technology","level":3,"score":0.44776660203933716},{"id":"https://openalex.org/C150903083","wikidata":"https://www.wikidata.org/wiki/Q7108","display_name":"Biotechnology","level":1,"score":0.4336183965206146},{"id":"https://openalex.org/C62112901","wikidata":"https://www.wikidata.org/wiki/Q189159","display_name":"Cell growth","level":2,"score":0.4313507080078125},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3854144513607025},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3788917064666748},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3556310832500458},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.33725300431251526},{"id":"https://openalex.org/C515207424","wikidata":"https://www.wikidata.org/wiki/Q8066","display_name":"Amino acid","level":2,"score":0.0},{"id":"https://openalex.org/C151730666","wikidata":"https://www.wikidata.org/wiki/Q7205","display_name":"Paleontology","level":1,"score":0.0},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[],"locations_count":3,"locations":[{"id":"pmh:http://www.rian.ie/86759/","doi":null,"is_oa":true,"landing_page_url":"http://doras.dcu.ie/19727/","pdf_url":"http://doras.dcu.ie/19727/","source":{"id":"https://openalex.org/S4306400033","display_name":"Arrow@dit (Dublin Institute of Technology)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4210144925","host_organization_name":"Technological University Dublin","host_organization_lineage":["https://openalex.org/I4210144925"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Byrne, Karen (2014) High density CHO cell cultures: Improved productivity and product quality. PhD thesis, Dublin City University.","raw_type":"Other"},{"id":"pmh:oai:doras.dcu.ie:19727","doi":null,"is_oa":true,"landing_page_url":"https://doras.dcu.ie/19727/1/Karen_Byrne_PhD_hardcopy_January_2014.pdf","pdf_url":"http://doras.dcu.ie/19727/1/Karen_Byrne_PhD_hardcopy_January_2014.pdf","source":{"id":"https://openalex.org/S4306401511","display_name":"Dublin City University Open Access Institutional Repository (Dublin City University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I42934936","host_organization_name":"Dublin City University","host_organization_lineage":["https://openalex.org/I42934936"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Thesis"},{"id":"mag:91937263","doi":null,"is_oa":false,"landing_page_url":"http://doras.dcu.ie/19727/1/Karen_Byrne_PhD_hardcopy_January_2014.pdf","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"pmh:http://www.rian.ie/86759/","doi":null,"is_oa":true,"landing_page_url":"http://doras.dcu.ie/19727/","pdf_url":"http://doras.dcu.ie/19727/","source":{"id":"https://openalex.org/S4306400033","display_name":"Arrow@dit (Dublin Institute of Technology)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4210144925","host_organization_name":"Technological University Dublin","host_organization_lineage":["https://openalex.org/I4210144925"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Byrne, Karen (2014) High density CHO cell cultures: Improved productivity and product quality. PhD thesis, Dublin City University.","raw_type":"Other"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320321056","display_name":"Irish Research Council","ror":"https://ror.org/051xex213"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W91937263.pdf","grobid_xml":"https://content.openalex.org/works/W91937263.grobid-xml"},"referenced_works_count":87,"referenced_works":["https://openalex.org/W1513389095","https://openalex.org/W1535034058","https://openalex.org/W1543319315","https://openalex.org/W1573653133","https://openalex.org/W1579357052","https://openalex.org/W1963538399","https://openalex.org/W1963546402","https://openalex.org/W1974786321","https://openalex.org/W1979068443","https://openalex.org/W1979650131","https://openalex.org/W1980674989","https://openalex.org/W1987743933","https://openalex.org/W1988515297","https://openalex.org/W1995570442","https://openalex.org/W1997336071","https://openalex.org/W1998912427","https://openalex.org/W2001500093","https://openalex.org/W2004979630","https://openalex.org/W2008907048","https://openalex.org/W2014720377","https://openalex.org/W2022211190","https://openalex.org/W2025433912","https://openalex.org/W2026370963","https://openalex.org/W2032064376","https://openalex.org/W2038802014","https://openalex.org/W2039966214","https://openalex.org/W2040312499","https://openalex.org/W2046431138","https://openalex.org/W2047981951","https://openalex.org/W2050698012","https://openalex.org/W2052465958","https://openalex.org/W2060076600","https://openalex.org/W2060545673","https://openalex.org/W2060908635","https://openalex.org/W2061311359","https://openalex.org/W2066603840","https://openalex.org/W2068044041","https://openalex.org/W2069349248","https://openalex.org/W2071888779","https://openalex.org/W2073497702","https://openalex.org/W2074335660","https://openalex.org/W2074589683","https://openalex.org/W2074989160","https://openalex.org/W2077386203","https://openalex.org/W2077924848","https://openalex.org/W2078705620","https://openalex.org/W2079126553","https://openalex.org/W2079776446","https://openalex.org/W2081640562","https://openalex.org/W2082840689","https://openalex.org/W2083780169","https://openalex.org/W2089304624","https://openalex.org/W2090690774","https://openalex.org/W2093343938","https://openalex.org/W2093658922","https://openalex.org/W2093910499","https://openalex.org/W2094337931","https://openalex.org/W2098201181","https://openalex.org/W2099855228","https://openalex.org/W2105796483","https://openalex.org/W2106785697","https://openalex.org/W2115970730","https://openalex.org/W2117521263","https://openalex.org/W2118540273","https://openalex.org/W2121600510","https://openalex.org/W2123907450","https://openalex.org/W2125066452","https://openalex.org/W2125907649","https://openalex.org/W2128735483","https://openalex.org/W2128761336","https://openalex.org/W2134639764","https://openalex.org/W2135087092","https://openalex.org/W2139132159","https://openalex.org/W2147730867","https://openalex.org/W2149298785","https://openalex.org/W2153486031","https://openalex.org/W2154110799","https://openalex.org/W2155927328","https://openalex.org/W2157766920","https://openalex.org/W2158246101","https://openalex.org/W2161270865","https://openalex.org/W2164441383","https://openalex.org/W2168834542","https://openalex.org/W2171877643","https://openalex.org/W2276236854","https://openalex.org/W2325074859","https://openalex.org/W3028906519"],"related_works":["https://openalex.org/W2203472963","https://openalex.org/W7212215454","https://openalex.org/W6945916133","https://openalex.org/W3153500354","https://openalex.org/W2073412042","https://openalex.org/W2157531491","https://openalex.org/W2784294288","https://openalex.org/W2253757383","https://openalex.org/W3198531740","https://openalex.org/W7013993708"],"abstract_inverted_index":{"Continual":[0],"growth":[1,139,278,376],"in":[2,6,12,22,38,63,101,119,230,242,280,302,329,342,357,378,383,409],"the":[3,23,61,68,114,126,133,148,151,178,181,196,207,210,266,269,273,276,294,315,343,358,371,374,379,397,404,414],"biopharmaceutical":[4],"industry":[5],"recombinant":[7,103,127,185,340],"protein":[8,90,104,128,186,260,270,341],"production":[9,19],"has":[10,96,250],"resulted":[11],"an":[13,303],"ever":[14],"increasing":[15],"demand":[16],"for":[17,45,67,137,144,218,238],"optimised":[18],"processes,":[20],"particularly":[21],"area":[24],"of":[25,35,47,73,87,116,135,147,180,198,209,216,268,275,339,368,373,391,399,406,416],"upstream":[26],"mammalian":[27,36,221],"cell":[28,48,79,123,138,231,296,320,393],"culture":[29,39,49,382],"bioprocessing.":[30],"The":[31,51,336,362,389],"inefficient":[32],"energy":[33],"demands":[34],"cells":[37,222,418],"persists":[40],"as":[41,213],"a":[42,214,306,330,365,407],"limiting":[43],"factor":[44],"optimisation":[46],"processes.":[50],"Process":[52],"Analytical":[53],"Technology":[54],"(PAT)":[55],"framework":[56],"initiative,":[57],"put":[58],"forward":[59],"by":[60,157,191,403],"FDA":[62],"2001,":[64],"outlines":[65],"measures":[66],"strict":[69],"monitoring":[70,392],"and":[71,82,140,168,233,283,298,312,395,419],"control":[72],"critical":[74],"process":[75],"parameters":[76],"(CPP-s)":[77],"affecting":[78,89],"growth,":[80],"productivity":[81],"product":[83,234,299],"quality.":[84,261],"\\n\\nThe":[85],"identification":[86,118],"CPP-s":[88],"quality":[91,187,267],"(namely":[92],"glycosylation":[93],"and/or":[94],"aggregation)":[95],"never":[97],"been":[98],"more":[99],"important":[100],"seeking":[102],"drug":[105],"approval.":[106],"A":[107],"project":[108],"was":[109,155,223,286,310,327,347,401],"designed":[110,311],"aimed":[111],"at":[112,272,370],"addressing":[113,206],"issue":[115],"CPP":[117],"high":[120,199],"density":[121],"CHO":[122],"cultures":[124,159,165,241,285,333,346],"producing":[125],"IgG1.":[129],"Initial":[130,262],"studies":[131,263],"identified":[132,156,176,264],"importance":[134,390],"L-glutamine":[136],"productivity,":[141],"but":[142],"also":[143,175,224],"increased":[145,228,319],"complexity":[146,369],"glycoform":[149],"on":[150,184,259],"IgG1":[152],"protein.":[153],"This":[154],"comparing":[158],"containing":[160,166],"4":[161,169],"mM":[162,170],"L-Glutamine":[163],"to":[164,244,248,252,291,314,349,385],"0":[167],"L-glutamate.":[171],"Interestingly":[172],"this":[173],"study":[174],"that":[177,265,325,355],"impact":[179],"by-product":[182],"ammonia":[183,201],"may":[188,256],"be":[189,350],"over-estimated":[190],"investigating":[192],"such":[193],"effects":[194],"through":[195],"incorporation":[197],"initial":[200],"concentrations":[202],"(~15":[203],"mM).":[204],"\\n\\nIn":[205],"title":[208],"project,":[211],"encapsulation":[212,255],"mode":[215],"cultivation":[217],"suspension":[219,245,284,331],"adapted":[220],"investigated,":[225],"with":[226,413],"significantly":[227],"yields":[229,297],"(3.7-fold)":[232],"titres":[235,300],"being":[236],"achieved":[237,328,356],"batch":[239,282,332,359,386],"encapsulated":[240,304,316,360,387],"comparison":[243,384],"cultures.":[246,361,388],"Research":[247],"date":[249],"failed":[251],"identify":[253],"if":[254],"have":[257],"implications":[258],"harvested":[271],"end":[274,372],"stationary":[277],"period":[279,377],"both":[281],"relatively":[287],"similar.":[288],"In":[289],"order":[290],"further":[292],"increase":[293],"maximum":[295],"obtained":[301],"culture,":[305],"control-fed":[307,344,380],"perfusion":[308,345,381,400],"strategy":[309],"applied":[313],"cells.":[317],"Further":[318],"yields,":[321],"10-fold":[322],"higher":[323,353,366],"than":[324,354],"which":[326],"were":[334],"noted.":[335],"volumetric":[337],"titre":[338],"determined":[348],"~2.65-":[351],"fold":[352],"rIgG1":[363],"had":[364],"degree":[367],"extended":[375],"viability":[394],"optimising":[396],"rate":[398],"noted":[402],"occurrence":[405],"decrease":[408],"glycan":[410],"complexity,":[411],"associated":[412],"accumulation":[415],"dead":[417],"glycosidase":[420],"release.":[421]},"counts_by_year":[{"year":2015,"cited_by_count":1}],"updated_date":"2026-11-11T12:16:26.678958","created_date":"2016-06-24T00:00:00"}
