{"id":"https://openalex.org/W91563351","doi":null,"title":"Lipid foulant interactions during the chromatographic purification of virus-like particles from Saccharomyces cerevisiae","display_name":"Lipid foulant interactions during the chromatographic purification of virus-like particles from Saccharomyces cerevisiae","publication_year":2011,"publication_date":"2011-02-28","ids":{"openalex":"https://openalex.org/W91563351","mag":"91563351"},"language":"en","primary_location":{"id":"pmh:oai:eprints.ucl.ac.uk.OAI2:1302065","doi":null,"is_oa":false,"landing_page_url":"http://discovery.ucl.ac.uk/1302065/","pdf_url":null,"source":{"id":"https://openalex.org/S4306400024","display_name":"UCL Discovery (University College London)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I45129253","host_organization_name":"University College London","host_organization_lineage":["https://openalex.org/I45129253"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Doctoral thesis, UCL (University College London).","raw_type":"Thesis"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5112021902","display_name":"Jun Jin","orcid":"https://orcid.org/0009-0005-7755-4647","observed_orcids":["https://orcid.org/0009-0005-7755-4647"]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Jun Jin","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5112021902"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":4,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.9932000041007996,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.9932000041007996,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10908","display_name":"Analytical Chemistry and Chromatography","score":0.0015999999595806003,"subfield":{"id":"https://openalex.org/subfields/1602","display_name":"Analytical Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T13128","display_name":"Chromatography in Natural Products","score":0.000699999975040555,"subfield":{"id":"https://openalex.org/subfields/1602","display_name":"Analytical Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/hydrophobic-interaction-chromatography","display_name":"hydrophobic interaction chromatography","score":0.9390000104904175},{"id":"https://openalex.org/keywords/virus-like-particles","display_name":"virus-like particles","score":0.9319999814033508},{"id":"https://openalex.org/keywords/hepatitis-b-surface-antigen","display_name":"hepatitis B surface antigen","score":0.9340000152587891},{"id":"https://openalex.org/keywords/saccharomyces-cerevisiae","display_name":"Saccharomyces cerevisiae","score":0.972000002861023},{"id":"https://openalex.org/keywords/triglycerides","display_name":"triglycerides","score":0.8320000171661377},{"id":"https://openalex.org/keywords/lipid-removal","display_name":"lipid removal","score":0.44200000166893005},{"id":"https://openalex.org/keywords/confocal-laser-scanning-microscopy","display_name":"confocal laser scanning microscopy","score":0.7239999771118164},{"id":"https://openalex.org/keywords/scanning-electron-microscopy","display_name":"scanning electron microscopy","score":0.6069999933242798},{"id":"https://openalex.org/keywords/vaccine-production","display_name":"vaccine production","score":0.4399999976158142},{"id":"https://openalex.org/keywords/feed-pretreatment","display_name":"feed pretreatment","score":0.6169999837875366}],"concepts":[{"id":"https://openalex.org/C150394285","wikidata":"https://www.wikidata.org/wiki/Q180254","display_name":"Adsorption","level":2,"score":0.7201401591300964},{"id":"https://openalex.org/C115792997","wikidata":"https://www.wikidata.org/wiki/Q1439803","display_name":"Fouling","level":3,"score":0.7164226770401001},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.7161237597465515},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5995309352874756},{"id":"https://openalex.org/C26771246","wikidata":"https://www.wikidata.org/wiki/Q321095","display_name":"Scanning electron microscope","level":2,"score":0.5413110256195068},{"id":"https://openalex.org/C42360764","wikidata":"https://www.wikidata.org/wiki/Q83588","display_name":"Chemical engineering","level":1,"score":0.41829657554626465},{"id":"https://openalex.org/C69357855","wikidata":"https://www.wikidata.org/wiki/Q163214","display_name":"Diffusion","level":2,"score":0.4118843376636505},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.329528272151947},{"id":"https://openalex.org/C41625074","wikidata":"https://www.wikidata.org/wiki/Q176088","display_name":"Membrane","level":2,"score":0.2563304901123047},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.15014424920082092},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.1088433563709259},{"id":"https://openalex.org/C127413603","wikidata":"https://www.wikidata.org/wiki/Q11023","display_name":"Engineering","level":0,"score":0.0},{"id":"https://openalex.org/C97355855","wikidata":"https://www.wikidata.org/wiki/Q11473","display_name":"Thermodynamics","level":1,"score":0.0},{"id":"https://openalex.org/C159985019","wikidata":"https://www.wikidata.org/wiki/Q181790","display_name":"Composite material","level":1,"score":0.0},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:eprints.ucl.ac.uk.OAI2:1302065","doi":null,"is_oa":false,"landing_page_url":"http://discovery.ucl.ac.uk/1302065/","pdf_url":null,"source":{"id":"https://openalex.org/S4306400024","display_name":"UCL Discovery (University College London)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I45129253","host_organization_name":"University College London","host_organization_lineage":["https://openalex.org/I45129253"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Doctoral thesis, UCL (University College London).","raw_type":"Thesis"},{"id":"mag:91563351","doi":null,"is_oa":false,"landing_page_url":"https://discovery.ucl.ac.uk/id/eprint/1302065/","pdf_url":null,"source":{"id":"https://openalex.org/S4306508231","display_name":"Doctoral thesis, UCL (University College London).","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"Doctoral thesis, UCL (University College London).","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":176,"referenced_works":["https://openalex.org/W32774942","https://openalex.org/W55714471","https://openalex.org/W59712658","https://openalex.org/W176413411","https://openalex.org/W383205146","https://openalex.org/W1175056953","https://openalex.org/W1482410339","https://openalex.org/W1488105682","https://openalex.org/W1505571782","https://openalex.org/W1517301454","https://openalex.org/W1533752660","https://openalex.org/W1548350437","https://openalex.org/W1550794048","https://openalex.org/W1554103893","https://openalex.org/W1559966695","https://openalex.org/W1575651020","https://openalex.org/W1581273677","https://openalex.org/W1603524454","https://openalex.org/W1604894604","https://openalex.org/W1605370140","https://openalex.org/W1750632522","https://openalex.org/W1964506638","https://openalex.org/W1965394699","https://openalex.org/W1965698866","https://openalex.org/W1967287809","https://openalex.org/W1967983350","https://openalex.org/W1968041165","https://openalex.org/W1969244240","https://openalex.org/W1970106113","https://openalex.org/W1970934632","https://openalex.org/W1971125664","https://openalex.org/W1971403261","https://openalex.org/W1971822930","https://openalex.org/W1972480731","https://openalex.org/W1973090022","https://openalex.org/W1974940588","https://openalex.org/W1976179441","https://openalex.org/W1977270347","https://openalex.org/W1977937489","https://openalex.org/W1981210320","https://openalex.org/W1983067261","https://openalex.org/W1984063294","https://openalex.org/W1984469411","https://openalex.org/W1984721194","https://openalex.org/W1985084275","https://openalex.org/W1985350532","https://openalex.org/W1985701662","https://openalex.org/W1986064647","https://openalex.org/W1987350137","https://openalex.org/W1987519563","https://openalex.org/W1988577957","https://openalex.org/W1989269139","https://openalex.org/W1989802949","https://openalex.org/W1990321791","https://openalex.org/W1990409075","https://openalex.org/W1990442609","https://openalex.org/W1991112845","https://openalex.org/W1991843046","https://openalex.org/W1992962372","https://openalex.org/W1994220086","https://openalex.org/W1994712533","https://openalex.org/W1994901499","https://openalex.org/W1995557023","https://openalex.org/W1997702098","https://openalex.org/W1998255597","https://openalex.org/W1999304511","https://openalex.org/W1999308566","https://openalex.org/W2000541359","https://openalex.org/W2001142804","https://openalex.org/W2003433691","https://openalex.org/W2004676082","https://openalex.org/W2006595934","https://openalex.org/W2006954969","https://openalex.org/W2007527667","https://openalex.org/W2009009104","https://openalex.org/W2010489161","https://openalex.org/W2010741267","https://openalex.org/W2010897495","https://openalex.org/W2012695091","https://openalex.org/W2013326293","https://openalex.org/W2017288352","https://openalex.org/W2017290048","https://openalex.org/W2019537104","https://openalex.org/W2021123040","https://openalex.org/W2022075663","https://openalex.org/W2023978004","https://openalex.org/W2025037117","https://openalex.org/W2025413405","https://openalex.org/W2026180733","https://openalex.org/W2026639106","https://openalex.org/W2027385153","https://openalex.org/W2030213603","https://openalex.org/W2033407070","https://openalex.org/W2033867130","https://openalex.org/W2033873040","https://openalex.org/W2034194003","https://openalex.org/W2035394735","https://openalex.org/W2035884729","https://openalex.org/W2038155287","https://openalex.org/W2038962652","https://openalex.org/W2039001047","https://openalex.org/W2040113564","https://openalex.org/W2040987932","https://openalex.org/W2041715180","https://openalex.org/W2044709482","https://openalex.org/W2044951291","https://openalex.org/W2046396005","https://openalex.org/W2046491869","https://openalex.org/W2047805891","https://openalex.org/W2051995369","https://openalex.org/W2052444922","https://openalex.org/W2052880114","https://openalex.org/W2053083611","https://openalex.org/W2055177700","https://openalex.org/W2056491293","https://openalex.org/W2056854152","https://openalex.org/W2057027424","https://openalex.org/W2057759640","https://openalex.org/W2057902340","https://openalex.org/W2058794164","https://openalex.org/W2059133672","https://openalex.org/W2061734833","https://openalex.org/W2061955290","https://openalex.org/W2062593377","https://openalex.org/W2066653994","https://openalex.org/W2066879136","https://openalex.org/W2068067779","https://openalex.org/W2068536431","https://openalex.org/W2072782729","https://openalex.org/W2072985567","https://openalex.org/W2073839440","https://openalex.org/W2074249425","https://openalex.org/W2075015023","https://openalex.org/W2075515367","https://openalex.org/W2077092510","https://openalex.org/W2078995805","https://openalex.org/W2080379345","https://openalex.org/W2083530632","https://openalex.org/W2084887005","https://openalex.org/W2086633179","https://openalex.org/W2088438766","https://openalex.org/W2088869535","https://openalex.org/W2094377797","https://openalex.org/W2095530546","https://openalex.org/W2100800584","https://openalex.org/W2102502421","https://openalex.org/W2117208263","https://openalex.org/W2119464903","https://openalex.org/W2119494319","https://openalex.org/W2125404721","https://openalex.org/W2127570240","https://openalex.org/W2131925560","https://openalex.org/W2134821974","https://openalex.org/W2135379058","https://openalex.org/W2139324621","https://openalex.org/W2144989031","https://openalex.org/W2151167965","https://openalex.org/W2154602608","https://openalex.org/W2154604015","https://openalex.org/W2157528420","https://openalex.org/W2158248944","https://openalex.org/W2159174898","https://openalex.org/W2159831861","https://openalex.org/W2159985945","https://openalex.org/W2164591301","https://openalex.org/W2166653842","https://openalex.org/W2170632475","https://openalex.org/W2171134514","https://openalex.org/W2225230676","https://openalex.org/W2243510543","https://openalex.org/W2244029044","https://openalex.org/W2315168497","https://openalex.org/W2416277795","https://openalex.org/W2467523018","https://openalex.org/W2505714800","https://openalex.org/W2518100345"],"related_works":["https://openalex.org/W2171134514","https://openalex.org/W2035125758","https://openalex.org/W2035394735","https://openalex.org/W2001600177","https://openalex.org/W2064139096","https://openalex.org/W2102502421","https://openalex.org/W2508248412","https://openalex.org/W2094948539","https://openalex.org/W1972073067","https://openalex.org/W2308043675"],"abstract_inverted_index":{"The":[0,44,273],"objective":[1],"of":[2,10,18,38,59,78,98,151,180,207,216,222,244,251],"this":[3,33,242],"study":[4,57],"was":[5,26,113,193,209,226],"to":[6,31,56,115,211,240],"understand":[7,32],"the":[8,16,29,36,76,87,102,117,139,144,181,197,205,213,220,223,229,277,288],"mechanism":[9,206,243],"lipid":[11,99,152,191,217,265],"fouling":[12,58,153,208,246],"in":[13,75,119,249,280,284],"chromatography":[14,245],"through":[15],"investigation":[17],"a":[19,53,71,131,189,281],"hydrophobic":[20,141],"interaction":[21],"\\nchromatography":[22],"(HIC)":[23],"operation.":[24],"This":[25],"motivated":[27],"by":[28,228],"need":[30],"phenomenon":[34],"during":[35],"manufacture":[37],"biological":[39],"products":[40],"such":[41],"as":[42,70,143],"vaccines.":[43],"\\nsystematic":[45],"approach":[46],"and":[47,63,91,108,125,149,154,160,256],"novel":[48],"analytical":[49],"techniques":[50],"employed":[51,69],"create":[52],"unique":[54],"platform":[55],"other":[60],"chromatographic":[61],"adsorbents":[62],"process":[64],"feed":[65,103,254,275],"\\nmaterials.":[66],"HIC":[67,120,257],"is":[68],"primary":[72],"capture":[73],"step":[74],"purification":[77],"yeast":[79],"derived":[80],"hepatitis":[81],"R":[82],"surface":[83],"antigen":[84],"(HBsAg),":[85],"where":[86],"required":[88],"cell":[89],"disruption":[90],"detergent":[92],"liberation":[93],"steps":[94],"release":[95],"high":[96],"levels":[97],"content":[100],"into":[101,276],"stream.":[104],"From":[105],"lipid-":[106],"\\nrich":[107],"lipid-depleted":[109],"feedstocks,":[110],"comparative":[111],"analysis":[112],"able":[114],"quantify":[116],"deterioration":[118],"performance":[121],"(binding":[122],"capacities,":[123],"purities":[124],"recoveries)":[126],"under":[127,165],"\\nsuccessive":[128],"cycles.":[129,295],"Furthermore,":[130],"full":[132],"mass":[133],"balance":[134],"on":[135,157,196,202,253],"host":[136],"lipids":[137],"identified":[138],"highly":[140],"triacylglyceride":[142],"main":[145],"foulant.":[146],"Intra-particle":[147],"distribution":[148],"\\nprogression":[150],"its":[155],"effects":[156],"material":[158],"adsorption":[159],"diffusion":[161,231],"were":[162,247],"then":[163],"examined":[164],"confocal":[166],"laser":[167],"scanning":[168,175],"microscopy":[169,177],"(CLSM).":[170],"In":[171],"addition,":[172],"high-":[173],"\\nresolution":[174],"electron":[176],"(SEM)":[178],"images":[179],"fouled":[182],"bead":[183,199],"(after":[184],"40":[185,294],"cycles)":[186],"confirmed":[187],"that":[188],"thick":[190],"layer":[192,236],"building":[194],"up":[195],"outer":[198],"surface.":[200],"\\nBased":[201],"these":[203],"findings,":[204],"thought":[210],"be":[212],"rapid":[214],"accumulation":[215],"foulant":[218],"at":[219],"rim":[221],"bead,":[224],"which":[225],"aggravated":[227],"\\npossible":[230],"hindrance":[232],"resulting":[233],"from":[234],"multi":[235],"adsorption.":[237],"Finally,":[238],"pretreatments":[239],"reduce":[241],"evaluated":[248],"terms":[250],"improvement":[252],"quality":[255],"performance.":[258],"Selective":[259],"adsorbent":[260],"polystyrene":[261],"XAD-4":[262],"demonstrated":[263],"promising":[264],"removal":[266],"capabilities":[267],"with":[268],"satisfactory":[269],"HBsAg":[270],"VLP":[271],"recoveries.":[272],"improved":[274],"column":[278],"resulted":[279],"\\nthree-fold":[282],"increase":[283],"product":[285],"capacity,":[286],"whilst":[287],"overall":[289],"yield":[290],"remained":[291],"constant":[292],"over":[293]},"counts_by_year":[{"year":2021,"cited_by_count":1},{"year":2016,"cited_by_count":1},{"year":2013,"cited_by_count":2}],"updated_date":"2026-08-21T09:56:20.448147","created_date":"2016-06-24T00:00:00"}
