{"id":"https://openalex.org/W911783596","doi":null,"title":"Delivery of Exogenous Imaging Probes for Cellular Microscopy","display_name":"Delivery of Exogenous Imaging Probes for Cellular Microscopy","publication_year":2013,"publication_date":"2013-10-24","ids":{"openalex":"https://openalex.org/W911783596","mag":"911783596"},"language":"en","primary_location":{"id":"pmh:oai:figshare.com:article/10850147","doi":null,"is_oa":true,"landing_page_url":"https://figshare.com/articles/thesis/Delivery_of_Exogenous_Imaging_Probes_for_Cellular_Microscopy/10850147","pdf_url":"https://figshare.com/articles/thesis/Delivery_of_Exogenous_Imaging_Probes_for_Cellular_Microscopy/10850147","source":{"id":"https://openalex.org/S4377196282","display_name":"Figshare","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4210132348","host_organization_name":"Figshare (United Kingdom)","host_organization_lineage":["https://openalex.org/I4210132348"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Thesis"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://figshare.com/articles/thesis/Delivery_of_Exogenous_Imaging_Probes_for_Cellular_Microscopy/10850147","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5055381152","display_name":"Shabnam Mohandessi","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Shabnam Mohandessi (7916993)","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5055381152"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11279","display_name":"Lanthanide and Transition Metal Complexes","score":0.8964999914169312,"subfield":{"id":"https://openalex.org/subfields/2505","display_name":"Materials Chemistry"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T11279","display_name":"Lanthanide and Transition Metal Complexes","score":0.8964999914169312,"subfield":{"id":"https://openalex.org/subfields/2505","display_name":"Materials Chemistry"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10532","display_name":"Molecular Sensors and Ion Detection","score":0.04129999876022339,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10540","display_name":"Advanced Fluorescence Microscopy Techniques","score":0.033399999141693115,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/terbium-complexes","display_name":"terbium complexes","score":0.890999972820282},{"id":"https://openalex.org/keywords/luminescent-probes","display_name":"luminescent probes","score":0.6499999761581421},{"id":"https://openalex.org/keywords/cell-penetrating-peptides","display_name":"cell-penetrating peptides","score":0.8119999766349792},{"id":"https://openalex.org/keywords/hiv-tat-peptide","display_name":"HIV TAT peptide","score":0.8220000267028809},{"id":"https://openalex.org/keywords/dihydrofolate-reductase","display_name":"dihydrofolate reductase","score":0.8289999961853027},{"id":"https://openalex.org/keywords/escherichia-coli","display_name":"Escherichia coli","score":0.8379999995231628},{"id":"https://openalex.org/keywords/membrane-permeabilization","display_name":"membrane permeabilization","score":0.6859999895095825},{"id":"https://openalex.org/keywords/streptolysin-o","display_name":"streptolysin O","score":0.9010000228881836},{"id":"https://openalex.org/keywords/macropinocytosis","display_name":"macropinocytosis","score":0.828000009059906},{"id":"https://openalex.org/keywords/osmotic-lysis","display_name":"osmotic lysis","score":0.7860000133514404},{"id":"https://openalex.org/keywords/fluorescence-resonance-energy-transfer","display_name":"fluorescence resonance energy transfer","score":0.7680000066757202},{"id":"https://openalex.org/keywords/live-cell-imaging","display_name":"live-cell imaging","score":0.5249999761581421},{"id":"https://openalex.org/keywords/protein-labeling","display_name":"protein labeling","score":0.38600000739097595},{"id":"https://openalex.org/keywords/organic-fluorophores","display_name":"organic fluorophores","score":0.8050000071525574}],"concepts":[{"id":"https://openalex.org/C147080431","wikidata":"https://www.wikidata.org/wiki/Q1074953","display_name":"Microscopy","level":2,"score":0.5913308262825012},{"id":"https://openalex.org/C171250308","wikidata":"https://www.wikidata.org/wiki/Q11468","display_name":"Nanotechnology","level":1,"score":0.42359843850135803},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.3385798931121826},{"id":"https://openalex.org/C136229726","wikidata":"https://www.wikidata.org/wiki/Q327092","display_name":"Biomedical engineering","level":1,"score":0.3384609818458557},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.2056419849395752},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.17613789439201355}],"mesh":[],"locations_count":1,"locations":[{"id":"pmh:oai:figshare.com:article/10850147","doi":null,"is_oa":true,"landing_page_url":"https://figshare.com/articles/thesis/Delivery_of_Exogenous_Imaging_Probes_for_Cellular_Microscopy/10850147","pdf_url":"https://figshare.com/articles/thesis/Delivery_of_Exogenous_Imaging_Probes_for_Cellular_Microscopy/10850147","source":{"id":"https://openalex.org/S4377196282","display_name":"Figshare","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4210132348","host_organization_name":"Figshare (United Kingdom)","host_organization_lineage":["https://openalex.org/I4210132348"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Thesis"}],"best_oa_location":{"id":"pmh:oai:figshare.com:article/10850147","doi":null,"is_oa":true,"landing_page_url":"https://figshare.com/articles/thesis/Delivery_of_Exogenous_Imaging_Probes_for_Cellular_Microscopy/10850147","pdf_url":"https://figshare.com/articles/thesis/Delivery_of_Exogenous_Imaging_Probes_for_Cellular_Microscopy/10850147","source":{"id":"https://openalex.org/S4377196282","display_name":"Figshare","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4210132348","host_organization_name":"Figshare (United Kingdom)","host_organization_lineage":["https://openalex.org/I4210132348"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Thesis"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W911783596.pdf","grobid_xml":"https://content.openalex.org/works/W911783596.grobid-xml"},"referenced_works_count":123,"referenced_works":["https://openalex.org/W1515068677","https://openalex.org/W1518543166","https://openalex.org/W1563836657","https://openalex.org/W1584683177","https://openalex.org/W1860685051","https://openalex.org/W1963819255","https://openalex.org/W1964963882","https://openalex.org/W1966649591","https://openalex.org/W1970270735","https://openalex.org/W1970973269","https://openalex.org/W1971276614","https://openalex.org/W1973562367","https://openalex.org/W1973712303","https://openalex.org/W1973920331","https://openalex.org/W1974186613","https://openalex.org/W1974642741","https://openalex.org/W1976223354","https://openalex.org/W1976927357","https://openalex.org/W1979129343","https://openalex.org/W1982301681","https://openalex.org/W1984470696","https://openalex.org/W1987349623","https://openalex.org/W1990388991","https://openalex.org/W1991720510","https://openalex.org/W1992834159","https://openalex.org/W1993914603","https://openalex.org/W2000231468","https://openalex.org/W2000997995","https://openalex.org/W2001980248","https://openalex.org/W2004334950","https://openalex.org/W2004335248","https://openalex.org/W2004390233","https://openalex.org/W2007341156","https://openalex.org/W2007457382","https://openalex.org/W2007971802","https://openalex.org/W2009640317","https://openalex.org/W2010804128","https://openalex.org/W2012492137","https://openalex.org/W2015704490","https://openalex.org/W2015835531","https://openalex.org/W2016036293","https://openalex.org/W2019144805","https://openalex.org/W2027045807","https://openalex.org/W2029346749","https://openalex.org/W2029932629","https://openalex.org/W2032105738","https://openalex.org/W2036076986","https://openalex.org/W2036648499","https://openalex.org/W2036730941","https://openalex.org/W2036856449","https://openalex.org/W2038074501","https://openalex.org/W2039702376","https://openalex.org/W2040336234","https://openalex.org/W2041690080","https://openalex.org/W2045606418","https://openalex.org/W2048470673","https://openalex.org/W2052706007","https://openalex.org/W2057001301","https://openalex.org/W2057141116","https://openalex.org/W2059234830","https://openalex.org/W2064124615","https://openalex.org/W2064726682","https://openalex.org/W2065770997","https://openalex.org/W2066848489","https://openalex.org/W2067173634","https://openalex.org/W2075974843","https://openalex.org/W2076859770","https://openalex.org/W2077102218","https://openalex.org/W2077525285","https://openalex.org/W2078873019","https://openalex.org/W2079335891","https://openalex.org/W2081457168","https://openalex.org/W2082454728","https://openalex.org/W2082468518","https://openalex.org/W2090682502","https://openalex.org/W2090888546","https://openalex.org/W2093274173","https://openalex.org/W2094492208","https://openalex.org/W2100042879","https://openalex.org/W2105608576","https://openalex.org/W2105668301","https://openalex.org/W2110367525","https://openalex.org/W2111593103","https://openalex.org/W2114733818","https://openalex.org/W2116647871","https://openalex.org/W2121134942","https://openalex.org/W2123650819","https://openalex.org/W2124639076","https://openalex.org/W2125449455","https://openalex.org/W2125450037","https://openalex.org/W2126629698","https://openalex.org/W2127068865","https://openalex.org/W2128873134","https://openalex.org/W2133466768","https://openalex.org/W2134178604","https://openalex.org/W2138857126","https://openalex.org/W2141717567","https://openalex.org/W2144147853","https://openalex.org/W2144175668","https://openalex.org/W2144196643","https://openalex.org/W2144259254","https://openalex.org/W2148924929","https://openalex.org/W2154008781","https://openalex.org/W2154321074","https://openalex.org/W2155935463","https://openalex.org/W2157456092","https://openalex.org/W2158821132","https://openalex.org/W2159106985","https://openalex.org/W2160863389","https://openalex.org/W2162076458","https://openalex.org/W2162355697","https://openalex.org/W2165217966","https://openalex.org/W2165752948","https://openalex.org/W2166168429","https://openalex.org/W2167806590","https://openalex.org/W2168816122","https://openalex.org/W2169594496","https://openalex.org/W2170477767","https://openalex.org/W2172232338","https://openalex.org/W2221020875","https://openalex.org/W2316132154","https://openalex.org/W2415768383","https://openalex.org/W3148913165"],"related_works":["https://openalex.org/W2765643444","https://openalex.org/W2765788739","https://openalex.org/W2886897819","https://openalex.org/W2046624890","https://openalex.org/W2584550153","https://openalex.org/W559995050","https://openalex.org/W2052114294","https://openalex.org/W2318921982","https://openalex.org/W2609080282","https://openalex.org/W7161796752"],"abstract_inverted_index":{"Optical":[0],"microscopy":[1,234],"has":[2,72],"emerged":[3],"as":[4,53,140],"one":[5],"of":[6,34,119,163,170,194,228,235,256,307,317,331,344,355,359,386,395],"the":[7,66,161,202,269,282,292,300,313,342,373,381,393],"most":[8,67],"important":[9],"methods":[10,150,174,263,279],"for":[11,85,142,167,353],"studying":[12],"living":[13],"cells":[14,166,219],"and":[15,45,82,132,225,248,260,277,304,319,333,363,369],"their":[16,35,111],"molecular":[17],"components.":[18],"Such":[19],"studies":[20],"require":[21],"fluorescent":[22,63,112,121,158,249],"probes":[23,94,141,159],"that":[24,124,151,186,264,280,376,383],"either":[25],"selectively":[26,187,209],"label":[27,188,210],"functional":[28],"biomolecules,":[29],"thereby":[30],"allowing":[31],"dynamic":[32],"tracking":[33],"sub-cellular":[36,43],"location,":[37],"or":[38,49,56,106,136,240],"else":[39],"partition":[40],"to":[41,99,104,129,138,155,177,201,208,287,322,338,378],"a":[42,116,195,356],"domain":[44],"report":[46],"particular":[47],"chemical":[48],"physical":[50,276],"properties":[51],"such":[52],"pH,":[54],"viscosity":[55],"calcium":[57],"ion":[58],"concentration.":[59],"While":[60],"genetically":[61],"encoded":[62],"proteins":[64,191,217],"are":[65,126,133,175],"common":[68],"probe":[69,258],"type,":[70],"there":[71],"been":[73,266],"considerable":[74],"interest":[75],"in":[76,87,192,268,274,285,372],"developing":[77],"organic":[78],"fluorophores,":[79],"metal":[80],"complexes":[81,181],"inorganic":[83],"nanoparticles":[84],"use":[86,139],"live":[88,143,396],"cell":[89,130,144,324,346],"imaging":[90,145,169],"studies.":[91,146],"These":[92],"exogenous":[93],"can":[95,152],"be":[96,100,153,351],"synthetically":[97],"modified":[98],"brighter,":[101],"more":[102,107],"resistant":[103],"photobleaching":[105],"analytically":[108],"responsive":[109],"than":[110],"protein":[113,171],"analogs.":[114],"However,":[115],"key":[117],"limitation":[118],"many":[120],"species":[122],"is":[123,328],"they":[125],"often":[127],"impermeant":[128],"membranes":[131],"therefore":[134],"difficult":[135],"impossible":[137],"This":[147],"dissertation":[148],"describes":[149,273],"used":[154],"deliver":[156,178,288],"membrane-impermeant,":[157],"into":[160,291,341,392],"cytoplasm":[162,293,343,394],"cultured":[164],"mammalian":[165],"microscopic":[168],"function.":[172],"Specifically,":[173],"described":[176,267],"luminescent":[179,257],"terbium":[180,197,238,247,289],"with":[182,299],"long":[183],"emission":[184,239],"lifetimes":[185],"recombinant":[189],"fusion":[190,216],"cellulo.\\nHeterodimers":[193],"2-hydroxyisophthalamide":[196],"macrocycle":[198],"(Lumi4-Tb\uf0e4)":[199],"linked":[200],"antibiotic":[203],"trimethoprim":[204],"(TMP)":[205],"were":[206,230,336],"observed":[207,337],"Escherichia":[211],"coli":[212],"dihydrofolate":[213],"reductase":[214],"(eDHFR)":[215],"inside":[218],"following":[220],"cytoplasmic":[221,315],"delivery.":[222],"Cellular":[223],"uptake":[224],"specific":[226],"labeling":[227],"eDHFR":[229],"visualized":[231],"using":[232],"time-gated":[233],"long-lived":[236],"(~ms)":[237],"F\u00f6rster":[241],"resonance":[242],"energy":[243],"transfer":[244],"(FRET)":[245],"between":[246],"proteins.":[250],"Chapter":[251,271,364],"1":[252],"provides":[253],"an":[254],"overview":[255],"types":[259],"intracellular":[261],"delivery":[262,316,349,354],"have":[265],"literature.":[270],"2":[272],"detail":[275],"biochemical":[278],"breach":[281],"lipid":[283],"bilayer":[284],"order":[286],"labels":[290],"including":[294],"microinjection,":[295],"reversible":[296],"membrane":[297],"permeabilization":[298],"bacterial":[301],"toxin":[302],"streptolysin-O":[303],"osmotic":[305],"lysis":[306],"macropinocytic":[308],"vesicles.":[309],"In":[310],"chapter":[311],"3,":[312],"successful":[314],"Lumi4":[318],"TMP-Lumi4":[320],"conjugated":[321],"arginine-rich,":[323],"penetrating":[325],"peptides":[326],"(CPPs)":[327],"described.":[329],"Conjugates":[330],"nonaarginine":[332],"HIV-tat-derived":[334],"sequences":[335],"directly":[339],"translocate":[340],"various":[345],"types.":[347],"CPP-mediated":[348],"should":[350],"adaptable":[352],"wide":[357],"variety":[358],"small":[360],"molecule":[361],"probes,":[362],"4":[365],"outlines":[366],"recent":[367],"data":[368],"ongoing":[370],"research":[371],"Miller":[374],"laboratory":[375],"aims":[377],"quantitatively":[379],"understand":[380],"factors":[382],"control":[384],"translocation":[385],"CPP-probe":[387],"conjugates":[388],"from":[389],"extracellular":[390],"medium":[391],"cells.":[397]},"counts_by_year":[],"updated_date":"2026-09-11T09:22:31.653822","created_date":"2025-10-10T00:00:00"}