{"id":"https://openalex.org/W8963448","doi":"https://doi.org/10.1007/978-1-59745-536-7_14","title":"Hematopoietic Colony-Forming Cell Assays","display_name":"Hematopoietic Colony-Forming Cell Assays","publication_year":2007,"publication_date":"2007-01-01","ids":{"openalex":"https://openalex.org/W8963448","doi":"https://doi.org/10.1007/978-1-59745-536-7_14","mag":"8963448","pmid":"https://pubmed.ncbi.nlm.nih.gov/18453257"},"language":"en","primary_location":{"id":"doi:10.1007/978-1-59745-536-7_14","doi":"https://doi.org/10.1007/978-1-59745-536-7_14","is_oa":false,"landing_page_url":"https://doi.org/10.1007/978-1-59745-536-7_14","pdf_url":null,"source":{"id":"https://openalex.org/S4210172139","display_name":"Methods in molecular biology","issn_l":"1064-3745","issn":["1064-3745","1940-6029"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3","vabb-shw"],"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"book series"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in Molecular Biology","raw_type":"book-chapter"},"volume":{"id":"https://openalex.org/W653801100","display_name":"Stem Cell Assays"},"type":"book-chapter","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5113464902","display_name":"Carla Chaves Pereira","orcid":"https://orcid.org/0009-0000-6492-2133","observed_orcids":["https://orcid.org/0009-0000-6492-2133"]},"institutions":[{"id":"https://openalex.org/I2801388136","display_name":"Stemcell Technologies","ror":"https://ror.org/04rsexf17","country_code":"CA","type":"company","lineage":["https://openalex.org/I2801388136"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Carla Pereira","raw_affiliation_strings":["StemCell Technologies, Vancouver, British Columbia, Canada","StemCell Technologies"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"StemCell Technologies, Vancouver, British Columbia, Canada","institution_ids":["https://openalex.org/I2801388136"]},{"raw_affiliation_string":"StemCell Technologies","institution_ids":["https://openalex.org/I2801388136"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5110489648","display_name":"Emer Clarke","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I2801388136","display_name":"Stemcell Technologies","ror":"https://ror.org/04rsexf17","country_code":"CA","type":"company","lineage":["https://openalex.org/I2801388136"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Emer Clarke","raw_affiliation_strings":["StemCell Technologies, Vancouver, Canada","StemCell Technologies"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"StemCell Technologies, Vancouver, Canada","institution_ids":["https://openalex.org/I2801388136"]},{"raw_affiliation_string":"StemCell Technologies","institution_ids":["https://openalex.org/I2801388136"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5111753982","display_name":"Jackie Damen","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I2801388136","display_name":"Stemcell Technologies","ror":"https://ror.org/04rsexf17","country_code":"CA","type":"company","lineage":["https://openalex.org/I2801388136"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Jackie Damen","raw_affiliation_strings":["StemCell Technologies, Vancouver, Canada","StemCell Technologies"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"StemCell Technologies, Vancouver, Canada","institution_ids":["https://openalex.org/I2801388136"]},{"raw_affiliation_string":"StemCell Technologies","institution_ids":["https://openalex.org/I2801388136"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I2801388136"],"apc_list":null,"apc_paid":null,"fwci":7.9482,"has_fulltext":false,"cited_by_count":45,"citation_normalized_percentile":{"value":0.9706499,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":99},"biblio":{"volume":"407","issue":null,"first_page":"177","last_page":"208"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10345","display_name":"Hematopoietic Stem Cell Transplantation","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10345","display_name":"Hematopoietic Stem Cell Transplantation","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10176","display_name":"Mesenchymal stem cell research","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10309","display_name":"Acute Myeloid Leukemia Research","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/hematopoietic-colony-forming-cells","display_name":"hematopoietic colony-forming cells","score":0.9539999961853027},{"id":"https://openalex.org/keywords/hematopoiesis","display_name":"hematopoiesis","score":0.9760000109672546},{"id":"https://openalex.org/keywords/hematopoietic-stem-cells","display_name":"hematopoietic stem cells","score":0.9760000109672546},{"id":"https://openalex.org/keywords/progenitor-cells","display_name":"progenitor cells","score":0.6880000233650208},{"id":"https://openalex.org/keywords/semi-solid-culture","display_name":"semi-solid culture","score":0.6299999952316284},{"id":"https://openalex.org/keywords/cytokines","display_name":"cytokines","score":0.7229999899864197},{"id":"https://openalex.org/keywords/erythroid-lineage","display_name":"erythroid lineage","score":0.8180000185966492},{"id":"https://openalex.org/keywords/myeloid-lineage","display_name":"myeloid lineage","score":0.9990000128746033},{"id":"https://openalex.org/keywords/lymphoid-lineage","display_name":"lymphoid lineage","score":0.9929999709129333},{"id":"https://openalex.org/keywords/megakaryocytic-lineage","display_name":"megakaryocytic lineage","score":0.9879999756813049},{"id":"https://openalex.org/keywords/bone-marrow","display_name":"bone marrow","score":0.5640000104904175},{"id":"https://openalex.org/keywords/in-vitro-assay","display_name":"in vitro assay","score":0.4429999887943268}],"concepts":[{"id":"https://openalex.org/C109159458","wikidata":"https://www.wikidata.org/wiki/Q919283","display_name":"Haematopoiesis","level":3,"score":0.7543988227844238},{"id":"https://openalex.org/C28328180","wikidata":"https://www.wikidata.org/wiki/Q48196","display_name":"Stem cell","level":2,"score":0.7059228420257568},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6979202032089233},{"id":"https://openalex.org/C201750760","wikidata":"https://www.wikidata.org/wiki/Q1896218","display_name":"Progenitor cell","level":3,"score":0.6792798638343811},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.6378546357154846},{"id":"https://openalex.org/C145103041","wikidata":"https://www.wikidata.org/wiki/Q1151519","display_name":"Embryonic stem cell","level":3,"score":0.6049927473068237},{"id":"https://openalex.org/C2780007613","wikidata":"https://www.wikidata.org/wiki/Q546523","display_name":"Bone marrow","level":2,"score":0.5480912923812866},{"id":"https://openalex.org/C2779282312","wikidata":"https://www.wikidata.org/wiki/Q1956561","display_name":"Myeloid","level":2,"score":0.4837496280670166},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.48327142000198364},{"id":"https://openalex.org/C20875687","wikidata":"https://www.wikidata.org/wiki/Q2642600","display_name":"Adult stem cell","level":4,"score":0.4701918363571167},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.46735480427742004},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.4375561475753784},{"id":"https://openalex.org/C123012128","wikidata":"https://www.wikidata.org/wiki/Q5376430","display_name":"Endothelial stem cell","level":3,"score":0.17617979645729065},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.08689844608306885},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000882","descriptor_name":"Haplorhini","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002469","descriptor_name":"Cell Separation","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D002478","descriptor_name":"Cells, Cultured","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003114","descriptor_name":"Colony-Forming Units Assay","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D004285","descriptor_name":"Dogs","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006412","descriptor_name":"Hematopoietic Stem Cells","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":true},{"descriptor_ui":"D006412","descriptor_name":"Hematopoietic Stem Cells","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008214","descriptor_name":"Lymphocytes","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false},{"descriptor_ui":"D008214","descriptor_name":"Lymphocytes","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D008533","descriptor_name":"Megakaryocytes","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false},{"descriptor_ui":"D008533","descriptor_name":"Megakaryocytes","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D015672","descriptor_name":"Erythroid Precursor Cells","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false},{"descriptor_ui":"D015672","descriptor_name":"Erythroid Precursor Cells","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D015703","descriptor_name":"Antigens, CD","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D022423","descriptor_name":"Myeloid Cells","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false},{"descriptor_ui":"D022423","descriptor_name":"Myeloid Cells","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D041905","descriptor_name":"Erythroid Cells","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051381","descriptor_name":"Rats","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1007/978-1-59745-536-7_14","doi":"https://doi.org/10.1007/978-1-59745-536-7_14","is_oa":false,"landing_page_url":"https://doi.org/10.1007/978-1-59745-536-7_14","pdf_url":null,"source":{"id":"https://openalex.org/S4210172139","display_name":"Methods in molecular biology","issn_l":"1064-3745","issn":["1064-3745","1940-6029"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3","vabb-shw"],"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"book series"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in Molecular Biology","raw_type":"book-chapter"},{"id":"pmid:18453257","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/18453257","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in molecular biology (Clifton, N.J.)","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.46810001134872437}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":25,"referenced_works":["https://openalex.org/W132845342","https://openalex.org/W173976910","https://openalex.org/W1421046027","https://openalex.org/W1503450484","https://openalex.org/W1601249765","https://openalex.org/W1994005628","https://openalex.org/W2040827928","https://openalex.org/W2043298989","https://openalex.org/W2047954757","https://openalex.org/W2049423476","https://openalex.org/W2059251746","https://openalex.org/W2063486944","https://openalex.org/W2079021703","https://openalex.org/W2096978032","https://openalex.org/W2107425729","https://openalex.org/W2116355749","https://openalex.org/W2128780176","https://openalex.org/W2159483734","https://openalex.org/W2272268908","https://openalex.org/W2409324014","https://openalex.org/W2415576240","https://openalex.org/W2416778215","https://openalex.org/W2417985992","https://openalex.org/W2418425957","https://openalex.org/W4294702695"],"related_works":["https://openalex.org/W2285393345","https://openalex.org/W7095156730","https://openalex.org/W153421366","https://openalex.org/W4200570022","https://openalex.org/W4243494063","https://openalex.org/W2951290578","https://openalex.org/W4379112960","https://openalex.org/W1637482366","https://openalex.org/W1558063481","https://openalex.org/W4406934219"],"abstract_inverted_index":{"Hematopoiesis":[0],"is":[1],"the":[2,13,36,99,126,144,150],"process":[3,23],"by":[4,120],"which":[5],"stem":[6,53],"cells":[7,18,54,65,115],"divide":[8,106],"and":[9,29,42,51,55,70,107,133,146,152,159],"differentiate":[10,108],"to":[11,49,81,105],"produce":[12],"multiple":[14],"types":[15],"of":[16,112,128,154],"mature":[17,114],"found":[19],"in":[20,25,35,40,43,86,89,94],"blood.":[21],"The":[22],"begins":[24],"early":[26,56],"embryonic":[27],"development":[28],"continues":[30],"throughout":[31],"adult":[32],"life,":[33],"primarily":[34],"bone":[37],"marrow.":[38],"Various":[39],"vivo":[41],"vitro":[44,90],"assays":[45,67,77],"have":[46],"been":[47],"developed":[48],"detect":[50],"assess":[52],"multi-potential":[57,134],"progenitors.":[58],"While":[59],"highly":[60],"informative":[61],"about":[62],"primitive":[63],"hematopoietic":[64],"these":[66],"are":[68,103],"long":[69],"labour":[71],"intensive.":[72],"Alternatively,":[73],"colony-forming":[74],"cell":[75,135,139],"(CFC)":[76],"may":[78],"be":[79,118],"used":[80,148],"quantify":[82],"more":[83,113],"lineage-restricted":[84],"progenitors":[85],"a":[87,95,110],"simple":[88],"assay.":[91],"When":[92],"cultured":[93],"semi-solid":[96],"medium":[97],"containing":[98],"appropriate":[100],"cytokines,":[101],"CFCs":[102],"able":[104],"into":[109],"colony":[111],"that":[116],"can":[117],"detected":[119],"light":[121],"microscopy.":[122],"This":[123,141],"allows":[124],"for":[125,149],"quantification":[127],"erythroid,":[129],"myeloid,":[130],"lymphoid,":[131],"megakaryocytic,":[132],"lineages":[136],"from":[137,156],"various":[138],"sources.":[140],"chapter":[142],"outlines":[143],"materials":[145],"methods":[147],"culture":[151],"assessment":[153],"CFC":[155],"humans,":[157],"mice,":[158],"other":[160],"species.":[161]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":2},{"year":2022,"cited_by_count":3},{"year":2020,"cited_by_count":4},{"year":2019,"cited_by_count":4},{"year":2018,"cited_by_count":3},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":2},{"year":2015,"cited_by_count":7},{"year":2014,"cited_by_count":3},{"year":2013,"cited_by_count":3},{"year":2012,"cited_by_count":3}],"updated_date":"2026-11-12T12:28:57.059296","created_date":"2016-06-24T00:00:00"}