{"id":"https://openalex.org/W89056973","doi":"https://doi.org/10.1007/978-1-62703-287-2_4","title":"A Method for Conducting Highly Sensitive MicroRNA In Situ Hybridization and Immunohistochemical Analysis in Pancreatic Cancer","display_name":"A Method for Conducting Highly Sensitive MicroRNA In Situ Hybridization and Immunohistochemical Analysis in Pancreatic Cancer","publication_year":2012,"publication_date":"2012-12-13","ids":{"openalex":"https://openalex.org/W89056973","doi":"https://doi.org/10.1007/978-1-62703-287-2_4","mag":"89056973","pmid":"https://pubmed.ncbi.nlm.nih.gov/23359149"},"language":"en","primary_location":{"id":"doi:10.1007/978-1-62703-287-2_4","doi":"https://doi.org/10.1007/978-1-62703-287-2_4","is_oa":false,"landing_page_url":"https://doi.org/10.1007/978-1-62703-287-2_4","pdf_url":null,"source":{"id":"https://openalex.org/S4210172139","display_name":"Methods in molecular biology","issn_l":"1064-3745","issn":["1064-3745","1940-6029"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3","vabb-shw"],"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"book series"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in Molecular Biology","raw_type":"book-chapter"},"volume":{"id":"https://openalex.org/W4206798568","display_name":"Pancreatic Cancer"},"type":"book-chapter","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5086209074","display_name":"Lorenzo F. Sempere","orcid":"https://orcid.org/0000-0002-2150-0122","observed_orcids":["https://orcid.org/0000-0002-2150-0122"]},"institutions":[{"id":"https://openalex.org/I1289422878","display_name":"Dartmouth\u2013Hitchcock Medical Center","ror":"https://ror.org/00d1dhh09","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I107672454","https://openalex.org/I1289422878","https://openalex.org/I4390039337"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Lorenzo F. Sempere","raw_affiliation_strings":["Department of Medicine, Dartmouth Hitchcock Medical Center, Hanover, NH, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medicine, Dartmouth Hitchcock Medical Center, Hanover, NH, USA","institution_ids":["https://openalex.org/I1289422878"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5052428479","display_name":"Murray Korc","orcid":"https://orcid.org/0000-0003-1566-0899","observed_orcids":["https://orcid.org/0000-0003-1566-0899"]},"institutions":[{"id":"https://openalex.org/I107672454","display_name":"Dartmouth College","ror":"https://ror.org/049s0rh22","country_code":"US","type":"education","lineage":["https://openalex.org/I107672454"]},{"id":"https://openalex.org/I1289422878","display_name":"Dartmouth\u2013Hitchcock Medical Center","ror":"https://ror.org/00d1dhh09","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I107672454","https://openalex.org/I1289422878","https://openalex.org/I4390039337"]},{"id":"https://openalex.org/I4390039337","display_name":"Dartmouth Health","ror":"https://ror.org/01pa9ed26","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I107672454","https://openalex.org/I4390039337"]},{"id":"https://openalex.org/I4390039367","display_name":"Dartmouth Cancer Center","ror":"https://ror.org/044b05b34","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4390039367"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Murray Korc","raw_affiliation_strings":["Department of Medicine, Norris Cotton Cancer Center, Dartmouth Hitchcock Medical Center, Lebanon, NH, USA","Department of Pharmacology and Toxicology, Dartmouth Medical School, Hanover, NH, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medicine, Norris Cotton Cancer Center, Dartmouth Hitchcock Medical Center, Lebanon, NH, USA","institution_ids":["https://openalex.org/I1289422878","https://openalex.org/I4390039367"]},{"raw_affiliation_string":"Department of Pharmacology and Toxicology, Dartmouth Medical School, Hanover, NH, USA","institution_ids":["https://openalex.org/I107672454","https://openalex.org/I4390039337"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":4,"corresponding_author_ids":["https://openalex.org/A5052428479"],"corresponding_institution_ids":["https://openalex.org/I107672454","https://openalex.org/I1289422878","https://openalex.org/I4390039337","https://openalex.org/I4390039367"],"apc_list":null,"apc_paid":null,"fwci":15.8997,"has_fulltext":false,"cited_by_count":27,"citation_normalized_percentile":{"value":0.98773221,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":89,"max":99},"biblio":{"volume":"980","issue":null,"first_page":"43","last_page":"59"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10062","display_name":"MicroRNA in disease regulation","score":0.8920999765396118,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10062","display_name":"MicroRNA in disease regulation","score":0.8920999765396118,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.0551999993622303,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10231","display_name":"Pancreatic and Hepatic Oncology Research","score":0.010200000368058681,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/pancreatic-ductal-adenocarcinoma","display_name":"pancreatic ductal adenocarcinoma","score":0.9869999885559082},{"id":"https://openalex.org/keywords/formalin-fixed-paraffin-embedded-tissue","display_name":"formalin-fixed paraffin-embedded tissue","score":0.9010000228881836},{"id":"https://openalex.org/keywords/tyramide-signal-amplification","display_name":"tyramide signal amplification","score":0.9160000085830688},{"id":"https://openalex.org/keywords/immunohistochemistry","display_name":"immunohistochemistry","score":0.7919999957084656},{"id":"https://openalex.org/keywords/fluorescence-detection","display_name":"fluorescence detection","score":0.44999998807907104}],"concepts":[{"id":"https://openalex.org/C2780210213","wikidata":"https://www.wikidata.org/wiki/Q212961","display_name":"Pancreatic cancer","level":3,"score":0.7553158402442932},{"id":"https://openalex.org/C81439078","wikidata":"https://www.wikidata.org/wiki/Q2304142","display_name":"In situ hybridization","level":4,"score":0.7533013224601746},{"id":"https://openalex.org/C145059251","wikidata":"https://www.wikidata.org/wiki/Q310899","display_name":"microRNA","level":3,"score":0.7319799065589905},{"id":"https://openalex.org/C204232928","wikidata":"https://www.wikidata.org/wiki/Q899285","display_name":"Immunohistochemistry","level":2,"score":0.5947891473770142},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.564454197883606},{"id":"https://openalex.org/C24107716","wikidata":"https://www.wikidata.org/wiki/Q123619","display_name":"Nucleic acid","level":2,"score":0.4896869957447052},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.4729556739330292},{"id":"https://openalex.org/C2777542201","wikidata":"https://www.wikidata.org/wiki/Q1336182","display_name":"Fluorescence in situ hybridization","level":4,"score":0.45958131551742554},{"id":"https://openalex.org/C2777676563","wikidata":"https://www.wikidata.org/wiki/Q413665","display_name":"Horseradish peroxidase","level":3,"score":0.45064952969551086},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.4368916153907776},{"id":"https://openalex.org/C502942594","wikidata":"https://www.wikidata.org/wiki/Q3421914","display_name":"Cancer research","level":1,"score":0.402910977602005},{"id":"https://openalex.org/C121608353","wikidata":"https://www.wikidata.org/wiki/Q12078","display_name":"Cancer","level":2,"score":0.4014902710914612},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.2542340159416199},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.17859047651290894},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.1253197193145752},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.11270841956138611},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.092430979013443},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.07554462552070618},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.06256991624832153},{"id":"https://openalex.org/C30481170","wikidata":"https://www.wikidata.org/wiki/Q37748","display_name":"Chromosome","level":3,"score":0.0}],"mesh":[{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007150","descriptor_name":"Immunohistochemistry","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D007150","descriptor_name":"Immunohistochemistry","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D007150","descriptor_name":"Immunohistochemistry","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D010190","descriptor_name":"Pancreatic Neoplasms","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D010190","descriptor_name":"Pancreatic Neoplasms","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D010190","descriptor_name":"Pancreatic Neoplasms","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D010190","descriptor_name":"Pancreatic Neoplasms","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D010190","descriptor_name":"Pancreatic Neoplasms","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D010190","descriptor_name":"Pancreatic Neoplasms","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D017403","descriptor_name":"In Situ Hybridization","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D017403","descriptor_name":"In Situ Hybridization","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D017403","descriptor_name":"In Situ Hybridization","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D035683","descriptor_name":"MicroRNAs","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D035683","descriptor_name":"MicroRNAs","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D035683","descriptor_name":"MicroRNAs","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1007/978-1-62703-287-2_4","doi":"https://doi.org/10.1007/978-1-62703-287-2_4","is_oa":false,"landing_page_url":"https://doi.org/10.1007/978-1-62703-287-2_4","pdf_url":null,"source":{"id":"https://openalex.org/S4210172139","display_name":"Methods in molecular biology","issn_l":"1064-3745","issn":["1064-3745","1940-6029"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3","vabb-shw"],"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"book series"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in Molecular Biology","raw_type":"book-chapter"},{"id":"pmid:23359149","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/23359149","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in molecular biology (Clifton, N.J.)","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.8371999859809875,"id":"https://metadata.un.org/sdg/3"}],"sustainable_development_goals_aurora":[{"display_name":"Good health and well-being","score":0.5099999904632568,"id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G3528626926","display_name":"Dysregulation of TGF Beta Action Pancreatic Cancer","funder_award_id":"5r37ca075059-15","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"},{"id":"https://openalex.org/G5413867238","display_name":null,"funder_award_id":"CA-R21-133715","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"},{"id":"https://openalex.org/G570500292","display_name":null,"funder_award_id":"CA-R37-075059","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"},{"id":"https://openalex.org/G5953273125","display_name":"Role of microRNAs in genetic mouse models of pancreatic cancer","funder_award_id":"5r21ca135664-02","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"},{"id":"https://openalex.org/G6401636427","display_name":"microRNAs as novel Biomarkers for Pancreatic Ductal Adenocarcinoma","funder_award_id":"5r21ca133715-02","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"},{"id":"https://openalex.org/G8880155402","display_name":null,"funder_award_id":"CA-R21-135664","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"}],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337351","display_name":"National Cancer Institute","ror":"https://ror.org/040gcmg81"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":42,"referenced_works":["https://openalex.org/W94815550","https://openalex.org/W1584433563","https://openalex.org/W1965714535","https://openalex.org/W1973758000","https://openalex.org/W1981186391","https://openalex.org/W1981889510","https://openalex.org/W1985051759","https://openalex.org/W1993055783","https://openalex.org/W2011550114","https://openalex.org/W2014946489","https://openalex.org/W2019640214","https://openalex.org/W2020080445","https://openalex.org/W2023560058","https://openalex.org/W2032984680","https://openalex.org/W2035987476","https://openalex.org/W2037095626","https://openalex.org/W2042206438","https://openalex.org/W2044967196","https://openalex.org/W2051227958","https://openalex.org/W2056646351","https://openalex.org/W2066135299","https://openalex.org/W2075207018","https://openalex.org/W2077297619","https://openalex.org/W2079251691","https://openalex.org/W2084480556","https://openalex.org/W2098653030","https://openalex.org/W2099023480","https://openalex.org/W2100801124","https://openalex.org/W2102326716","https://openalex.org/W2107961632","https://openalex.org/W2126663928","https://openalex.org/W2136990999","https://openalex.org/W2137665434","https://openalex.org/W2139992910","https://openalex.org/W2140946995","https://openalex.org/W2147717419","https://openalex.org/W2152558372","https://openalex.org/W2162674813","https://openalex.org/W2163066658","https://openalex.org/W2171131178","https://openalex.org/W2415232078","https://openalex.org/W2560320833"],"related_works":["https://openalex.org/W2032125405","https://openalex.org/W2163066658","https://openalex.org/W2152036735","https://openalex.org/W2591335687","https://openalex.org/W2116348800","https://openalex.org/W2324423792","https://openalex.org/W4391756258","https://openalex.org/W4244426251","https://openalex.org/W2051227958","https://openalex.org/W2591222332"],"abstract_inverted_index":{"Profiling":[0],"experiments":[1],"in":[2,32,40,58,77,138],"whole":[3],"tissue":[4,81,141],"biopsies":[5],"have":[6],"linked":[7],"altered":[8,27],"expression":[9,29],"of":[10,16,104,122,149],"microRNAs":[11],"(miRNAs)":[12],"to":[13,48,70],"different":[14,33,114],"types":[15],"cancer,":[17],"including":[18],"pancreatic":[19,140],"ductal":[20],"adenocarcinoma":[21],"(PDAC).":[22],"Emerging":[23],"evidence":[24],"indicates":[25],"that":[26,119],"miRNA":[28,57,72],"can":[30],"occur":[31],"cellular":[34],"compartments":[35],"(cancer":[36],"and":[37,43,85,127,133,146],"non-cancer":[38],"cells)":[39],"tumor":[41],"lesions,":[42],"thus":[44],"it":[45],"is":[46],"important":[47],"ascertain":[49],"which":[50],"specific":[51],"cell":[52,134],"type":[53,135],"expresses":[54],"a":[55,64,96],"particulars":[56],"PDAC":[59],"tissues.":[60],"Here,":[61],"we":[62],"describe":[63,95],"highly":[65],"sensitive":[66],"fluorescence-based":[67],"ISH":[68],"method":[69,99],"visualize":[71],"accumulation":[73],"within":[74],"individual":[75],"cells":[76],"formalin-fixed":[78],"paraffin-embedded":[79],"(FFPE)":[80],"sections":[82,142],"using":[83],"5'":[84],"3'":[86],"terminally":[87],"fluorescein-labeled":[88],"locked":[89],"nucleic":[90],"acid":[91],"(LNA)-modified":[92],"probes.":[93],"We":[94],"multicolor":[97],"ISH/IHC":[98],"based":[100],"on":[101],"sequential":[102],"rounds":[103],"horseradish":[105],"peroxidase":[106],"(HRP)-mediated":[107],"tyramide":[108],"signal":[109,131],"amplification":[110],"(TSA)":[111],"reactions":[112],"with":[113],"in-house":[115],"synthesized":[116],"fluorochrome-conjugated":[117],"substrates":[118],"enable":[120],"co-detection":[121],"miRNAs,":[123],"abundant":[124],"noncoding":[125],"RNAs":[126],"protein":[128],"markers":[129],"for":[130],"quantification,":[132],"co-localization":[136],"studies":[137],"FFPE":[139],"from":[143],"clinical":[144],"specimens":[145],"mouse":[147],"models":[148],"PDAC.":[150]},"counts_by_year":[{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":2},{"year":2020,"cited_by_count":2},{"year":2018,"cited_by_count":2},{"year":2017,"cited_by_count":5},{"year":2016,"cited_by_count":3},{"year":2015,"cited_by_count":2},{"year":2014,"cited_by_count":8},{"year":2013,"cited_by_count":2}],"updated_date":"2026-10-27T15:51:10.671819","created_date":"2016-06-24T00:00:00"}