{"id":"https://openalex.org/W885377461","doi":null,"title":"Cloning and sequencing of reconstructed eukaryotic expression vector pIRES2-EGFP carrying CD and TRAIL genes","display_name":"Cloning and sequencing of reconstructed eukaryotic expression vector pIRES2-EGFP carrying CD and TRAIL genes","publication_year":2011,"publication_date":"2011-01-01","ids":{"openalex":"https://openalex.org/W885377461","mag":"885377461"},"language":"en","primary_location":{"id":"mag:2379586428","doi":null,"is_oa":false,"landing_page_url":"https://en.cnki.com.cn/Article_en/CJFDTOTAL-SDYY201101010.htm","pdf_url":null,"source":{"id":"https://openalex.org/S2764537232","display_name":"Shandong yiyao","issn_l":"1002-266X","issn":["1002-266X"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4361727546","host_organization_name":"Shandong Provincial Department of Health","host_organization_lineage":["https://openalex.org/P4361727546"],"host_organization_lineage_names":["Shandong Provincial Department of Health"],"type":"journal"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"Shandong yiyao","raw_type":null},"volume":null,"type":"article","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5089154171","display_name":"\u6881\u5175","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"\u6881\u5175","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5079786753","display_name":"\u8881\u82b3","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"\u8881\u82b3","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5060559432","display_name":"\u6bb7\u5efa\u745e","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"\u6bb7\u5efa\u745e","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5089844318","display_name":"\u8c2d\u4e3d\u534e","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"\u8c2d\u4e3d\u534e","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5035206448","display_name":"\u9ad8\u5e86\u6625","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"\u9ad8\u5e86\u6625","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5029367245","display_name":"\u9ad8\u806a","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"\u9ad8\u806a","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.07608177,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"51","issue":"1","first_page":"21","last_page":"23"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10129","display_name":"Glioma Diagnosis and Treatment","score":0.39430001378059387,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10129","display_name":"Glioma Diagnosis and Treatment","score":0.39430001378059387,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10613","display_name":"Virus-based gene therapy research","score":0.3158999979496002,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10294","display_name":"Cell death mechanisms and regulation","score":0.07779999822378159,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/eukaryotic-expression-vector-construction","display_name":"eukaryotic expression vector construction","score":0.859000027179718},{"id":"https://openalex.org/keywords/trail","display_name":"TRAIL","score":0.7820000052452087},{"id":"https://openalex.org/keywords/enhanced-green-fluorescent-protein","display_name":"enhanced green fluorescent protein","score":0.37700000405311584},{"id":"https://openalex.org/keywords/high-grade-glioma","display_name":"high-grade glioma","score":0.796999990940094},{"id":"https://openalex.org/keywords/combination-therapy","display_name":"combination therapy","score":0.6790000200271606}],"concepts":[{"id":"https://openalex.org/C142613039","wikidata":"https://www.wikidata.org/wiki/Q272983","display_name":"Green fluorescent protein","level":3,"score":0.7297648787498474},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4366218149662018},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3464493155479431},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.22698605060577393},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0773555338382721}],"mesh":[],"locations_count":2,"locations":[{"id":"mag:2379586428","doi":null,"is_oa":false,"landing_page_url":"https://en.cnki.com.cn/Article_en/CJFDTOTAL-SDYY201101010.htm","pdf_url":null,"source":{"id":"https://openalex.org/S2764537232","display_name":"Shandong yiyao","issn_l":"1002-266X","issn":["1002-266X"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4361727546","host_organization_name":"Shandong Provincial Department of Health","host_organization_lineage":["https://openalex.org/P4361727546"],"host_organization_lineage_names":["Shandong Provincial Department of Health"],"type":"journal"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"Shandong yiyao","raw_type":null},{"id":"mag:885377461","doi":null,"is_oa":false,"landing_page_url":"http://www.cqvip.com/QK/92062X/201101/36548362.html","pdf_url":null,"source":{"id":"https://openalex.org/S4306547874","display_name":"\u5c71\u4e1c\u533b\u836f","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"\u5c71\u4e1c\u533b\u836f","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.6240000128746033}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W1547306102","https://openalex.org/W1412992935","https://openalex.org/W2379586428","https://openalex.org/W2278432257","https://openalex.org/W760043067","https://openalex.org/W3012871360","https://openalex.org/W962366206","https://openalex.org/W1009249684","https://openalex.org/W63989289","https://openalex.org/W2273521561"],"abstract_inverted_index":{"\u76ee\u7684":[0],"\u6784\u5efa\u771f\u6838\u8868\u8fbe\u8f7d\u4f53plRES2-EGFP\uff0fTRAIL\u548cpinES2-EGFP\uff0fCD\uff0c\u4e3a\u7814\u7a76\u5176\u8054\u5408\u8868\u8fbe\u5bf9\u6076\u6027\u80f6\u8d28\u7624\u7684\u8054\u5408\u6cbb\u7597\u4f5c\u7528\u63d0\u4f9b\u57fa\u7840\u3002\u65b9\u6cd5\u5c06pCMV\uff0fCD\u8d28\u7c92\u548cpcDNA3\uff0e1\uff08\uff0b\uff09\uff0fTRAIL\u8d28\u7c92\u884c\u743c\u8102\u7cd6\u51dd\u80f6\u7535\u6cf3\u68c0\u6d4b\uff0c\u786e\u5b9a\u5176\u5b8c\u6574\u6027\uff0c\u5e76\u8fdb\u884c\u5e8f\u5217\u6d4b\u5b9a\u786e\u5b9a\u6709\u65e0\u57fa\u56e0\u7a81\u53d8\u3002pCMV\uff0fCD\u8d28\u7c92\u7528Sac\u2161\uff0fXho\u2160\u53cc\u9176\u5207\uff0cpcDNA3\uff0e1\uff08\uff0b\uff09TRAIL\u8d28\u7c92\u7528BamH\u2160\uff0fXho\u2160\u53cc\u9176\u5207\uff0c\u5c06\u76ee\u7684\u57fa\u56e0\u5b9a\u5411\u514b\u9686\u5230\u771f\u6838\u7ec6\u80de\u8868\u8fbe\u8f7d\u4f53plRES2-EGFP\u4e2d\uff0c\u8f6c\u5316E\uff0ecoliDH5\u03b1\u611f\u53d7\u6001\u7ec6\u80de\uff0c\u901a\u8fc7\u9650\u5236\u6027\u5185\u5207\u9176\u53cc\u9176\u5207\u3001PCR\u53ca\u6838\u9178\u5e8f\u5217\u5206\u6790\u7b49\u7b5b\u9009\u3001\u9274\u5b9a\u91cd\u7ec4\u8d28\u7c92\u3002\u7ed3\u679c\u6240\u6784\u5efa\u7684\u771f\u6838\u8868\u8fbe\u8f7d\u4f53plRES2-EGFP\uff0fTRAIL\u7ecfSac\u2161\uff0fXho\u2160\u53cc\u9176\u5207\u56de\u6536\u7247\u6bb5\u5206\u522b\u4e3a1\uff0e0\u30015\uff0e2kb\uff1bplRES2-EGFP\uff0fCD\u7ecfBamH\u2160\uff0fXho\u2160\u53cc\u9176\u5207\u56de\u6536\u7247\u6bb5\u5206\u522b\u4e3a1\uff0e3\u30015\uff0e2kb\u3002PCR\u53ca\u6d4b\u5e8f\u8bc1\u5b9e\uff0cplRES2-EGFP\uff0fTRAIL\u548cplRES2-EGFP\uff0fCD\u8d28\u7c92\u57fa\u56e0\u7ec4\u4e2d\u5206\u522b\u5305\u542bTRAIL\u548cCD\u57fa\u56e0\u3002\u7ed3\u8bba\u6210\u529f\u6784\u5efa\u4e86\u771f\u6838\u8868\u8fbe\u8f7d\u4f53plRES2-EGFP\uff0fTRAIL\u548cplRES2-EGFP\uff0fCD\uff0c\u4e3a\u7814\u7a76\u5176\u8054\u5408\u8868\u8fbe\u5bf9\u6076\u6027\u80f6\u8d28\u7624\u7684\u8054\u5408\u6cbb\u7597\u4f5c\u7528\u5960\u5b9a\u4e86\u57fa\u7840\u3002":[1]},"counts_by_year":[],"updated_date":"2026-11-05T12:25:35.769452","created_date":"2016-06-24T00:00:00"}