{"id":"https://openalex.org/W88043267","doi":null,"title":"Physiological aspects underpinning recombinant protein production in Escherichia coli","display_name":"Physiological aspects underpinning recombinant protein production in Escherichia coli","publication_year":2011,"publication_date":"2011-07-01","ids":{"openalex":"https://openalex.org/W88043267","mag":"88043267"},"language":"en","primary_location":{"id":"pmh:oai:etheses.bham.ac.uk:1384","doi":null,"is_oa":true,"landing_page_url":"http://etheses.bham.ac.uk/1384/1/Alfasi_PhD_10.pdf","pdf_url":"http://etheses.bham.ac.uk/1384/1/Alfasi_PhD_10.pdf","source":{"id":"https://openalex.org/S4306402357","display_name":"University of Birmingham Institutional Research Archive (University of Birmingham)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I79619799","host_organization_name":"University of Birmingham","host_organization_lineage":["https://openalex.org/I79619799"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Thesis"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"http://etheses.bham.ac.uk/1384/1/Alfasi_PhD_10.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5012146767","display_name":"Sara Alfasi","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Sara Alfasi","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5012146767"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":3,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.7592999935150146,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.7592999935150146,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.18930000066757202,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.012199999764561653,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/recombinant-protein-expression","display_name":"recombinant protein expression","score":0.9959999918937683},{"id":"https://openalex.org/keywords/escherichia-coli","display_name":"Escherichia coli","score":0.9440000057220459},{"id":"https://openalex.org/keywords/escherichia-coli-bl21-de3","display_name":"Escherichia coli BL21(DE3)","score":0.7960000038146973},{"id":"https://openalex.org/keywords/t7-rna-polymerase","display_name":"T7 RNA polymerase","score":0.8539999723434448},{"id":"https://openalex.org/keywords/iptg-induction","display_name":"IPTG induction","score":0.7590000033378601},{"id":"https://openalex.org/keywords/unfolded-protein-accumulation","display_name":"unfolded protein accumulation","score":0.7379999756813049},{"id":"https://openalex.org/keywords/inclusion-bodies","display_name":"inclusion bodies","score":0.9750000238418579},{"id":"https://openalex.org/keywords/outer-membrane-lipoprotein","display_name":"outer membrane lipoprotein","score":0.824999988079071},{"id":"https://openalex.org/keywords/cytosolic-proteins","display_name":"cytosolic proteins","score":0.8690000176429749},{"id":"https://openalex.org/keywords/stress-tolerance","display_name":"stress tolerance","score":0.453000009059906},{"id":"https://openalex.org/keywords/post-translational-modification","display_name":"post-translational modification","score":0.8949999809265137},{"id":"https://openalex.org/keywords/gfp-tagged-proteins","display_name":"GFP-tagged proteins","score":0.6729999780654907},{"id":"https://openalex.org/keywords/protein-folding","display_name":"protein folding","score":0.5400000214576721}],"concepts":[{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.7453020811080933},{"id":"https://openalex.org/C203750385","wikidata":"https://www.wikidata.org/wiki/Q1308970","display_name":"Inclusion bodies","level":4,"score":0.6982219219207764},{"id":"https://openalex.org/C2780048736","wikidata":"https://www.wikidata.org/wiki/Q418713","display_name":"T7 RNA polymerase","level":5,"score":0.6663572788238525},{"id":"https://openalex.org/C3675279","wikidata":"https://www.wikidata.org/wiki/Q211935","display_name":"Protein biosynthesis","level":2,"score":0.6474208235740662},{"id":"https://openalex.org/C170344550","wikidata":"https://www.wikidata.org/wiki/Q311359","display_name":"lac operon","level":4,"score":0.591325044631958},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.5601716041564941},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5038167834281921},{"id":"https://openalex.org/C2776449523","wikidata":"https://www.wikidata.org/wiki/Q272631","display_name":"RNA polymerase","level":4,"score":0.48864632844924927},{"id":"https://openalex.org/C2780430158","wikidata":"https://www.wikidata.org/wiki/Q7685902","display_name":"Target protein","level":3,"score":0.4595851004123688},{"id":"https://openalex.org/C139447449","wikidata":"https://www.wikidata.org/wiki/Q14633900","display_name":"Unfolded protein response","level":3,"score":0.4260832369327545},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.4152480363845825},{"id":"https://openalex.org/C205260736","wikidata":"https://www.wikidata.org/wiki/Q418939","display_name":"Heat shock protein","level":3,"score":0.41383984684944153},{"id":"https://openalex.org/C2781047461","wikidata":"https://www.wikidata.org/wiki/Q679692","display_name":"Biopharmaceutical","level":2,"score":0.4117787480354309},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.30876272916793823},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.2120911180973053},{"id":"https://openalex.org/C2776441376","wikidata":"https://www.wikidata.org/wiki/Q165028","display_name":"Bacteriophage","level":4,"score":0.12794950604438782},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.11329364776611328}],"mesh":[],"locations_count":4,"locations":[{"id":"pmh:oai:etheses.bham.ac.uk:1384","doi":null,"is_oa":true,"landing_page_url":"http://etheses.bham.ac.uk/1384/1/Alfasi_PhD_10.pdf","pdf_url":"http://etheses.bham.ac.uk/1384/1/Alfasi_PhD_10.pdf","source":{"id":"https://openalex.org/S4306402357","display_name":"University of Birmingham Institutional Research Archive (University of Birmingham)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I79619799","host_organization_name":"University of Birmingham","host_organization_lineage":["https://openalex.org/I79619799"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Thesis"},{"id":"pmh:hdl:10068/977751","doi":null,"is_oa":false,"landing_page_url":"http://hdl.handle.net/10068/977751","pdf_url":null,"source":{"id":"https://openalex.org/S4377196900","display_name":"OpenGrey (Institut de l'Information Scientifique et Technique)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1295345811","host_organization_name":"Institut de l'Information Scientifique et Technique","host_organization_lineage":["https://openalex.org/I1295345811"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"U - Thesis"},{"id":"pmh:oai:uilis.unsyiah.ac.id:43417","doi":null,"is_oa":false,"landing_page_url":"http://uilis.unsyiah.ac.id/opentheses/items/show/43417","pdf_url":null,"source":{"id":"https://openalex.org/S4377196378","display_name":"UPT. Syiah Kuala University Library (Syiah Kuala University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I187494767","host_organization_name":"Universitas Syiah Kuala","host_organization_lineage":["https://openalex.org/I187494767"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Thesis"},{"id":"mag:88043267","doi":null,"is_oa":false,"landing_page_url":"http://etheses.bham.ac.uk/1384/1/Alfasi_PhD_10.pdf","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"pmh:oai:etheses.bham.ac.uk:1384","doi":null,"is_oa":true,"landing_page_url":"http://etheses.bham.ac.uk/1384/1/Alfasi_PhD_10.pdf","pdf_url":"http://etheses.bham.ac.uk/1384/1/Alfasi_PhD_10.pdf","source":{"id":"https://openalex.org/S4306402357","display_name":"University of Birmingham Institutional Research Archive (University of Birmingham)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I79619799","host_organization_name":"University of Birmingham","host_organization_lineage":["https://openalex.org/I79619799"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Thesis"},"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.504800021648407}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320334629","display_name":"Biotechnology and Biological Sciences Research Council","ror":"https://ror.org/00cwqg982"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W88043267.pdf","grobid_xml":"https://content.openalex.org/works/W88043267.grobid-xml"},"referenced_works_count":134,"referenced_works":["https://openalex.org/W456191","https://openalex.org/W4366438","https://openalex.org/W72883546","https://openalex.org/W1497443366","https://openalex.org/W1497462806","https://openalex.org/W1519297291","https://openalex.org/W1539744755","https://openalex.org/W1708105888","https://openalex.org/W1789943808","https://openalex.org/W1873199501","https://openalex.org/W1897672732","https://openalex.org/W1940791100","https://openalex.org/W1951186315","https://openalex.org/W1961694262","https://openalex.org/W1963734207","https://openalex.org/W1965246206","https://openalex.org/W1965987341","https://openalex.org/W1966759409","https://openalex.org/W1968059097","https://openalex.org/W1970337460","https://openalex.org/W1970756887","https://openalex.org/W1972823382","https://openalex.org/W1973385732","https://openalex.org/W1973863718","https://openalex.org/W1975993749","https://openalex.org/W1978108559","https://openalex.org/W1980073547","https://openalex.org/W1980483593","https://openalex.org/W1984700929","https://openalex.org/W1987209219","https://openalex.org/W1988602371","https://openalex.org/W1992299163","https://openalex.org/W1998324446","https://openalex.org/W2000750361","https://openalex.org/W2000896683","https://openalex.org/W2003741495","https://openalex.org/W2005197694","https://openalex.org/W2005221143","https://openalex.org/W2009393325","https://openalex.org/W2010546547","https://openalex.org/W2010725868","https://openalex.org/W2014346478","https://openalex.org/W2014736760","https://openalex.org/W2014781338","https://openalex.org/W2023359102","https://openalex.org/W2023817805","https://openalex.org/W2024453737","https://openalex.org/W2026169768","https://openalex.org/W2030695915","https://openalex.org/W2032666701","https://openalex.org/W2032699674","https://openalex.org/W2036746284","https://openalex.org/W2039108743","https://openalex.org/W2039160511","https://openalex.org/W2042879396","https://openalex.org/W2043423695","https://openalex.org/W2044522314","https://openalex.org/W2046955172","https://openalex.org/W2047186187","https://openalex.org/W2050482128","https://openalex.org/W2051496342","https://openalex.org/W2052158605","https://openalex.org/W2055566311","https://openalex.org/W2056592058","https://openalex.org/W2058574181","https://openalex.org/W2058658881","https://openalex.org/W2060539823","https://openalex.org/W2069090007","https://openalex.org/W2069192891","https://openalex.org/W2070817124","https://openalex.org/W2074541085","https://openalex.org/W2075114517","https://openalex.org/W2076624186","https://openalex.org/W2077374889","https://openalex.org/W2078611252","https://openalex.org/W2079139546","https://openalex.org/W2081592039","https://openalex.org/W2082405411","https://openalex.org/W2082510875","https://openalex.org/W2086918731","https://openalex.org/W2088368476","https://openalex.org/W2088448530","https://openalex.org/W2088791106","https://openalex.org/W2089235241","https://openalex.org/W2089389960","https://openalex.org/W2093624850","https://openalex.org/W2095483871","https://openalex.org/W2104943438","https://openalex.org/W2105028012","https://openalex.org/W2109951903","https://openalex.org/W2113314251","https://openalex.org/W2115668757","https://openalex.org/W2116605034","https://openalex.org/W2117482139","https://openalex.org/W2121839643","https://openalex.org/W2126295762","https://openalex.org/W2126579809","https://openalex.org/W2126696746","https://openalex.org/W2129646968","https://openalex.org/W2131025983","https://openalex.org/W2131078078","https://openalex.org/W2132522708","https://openalex.org/W2132584326","https://openalex.org/W2136898237","https://openalex.org/W2140237950","https://openalex.org/W2141027030","https://openalex.org/W2141426247","https://openalex.org/W2146359135","https://openalex.org/W2148325536","https://openalex.org/W2148598076","https://openalex.org/W2149141532","https://openalex.org/W2150402850","https://openalex.org/W2151607788","https://openalex.org/W2152320286","https://openalex.org/W2152650892","https://openalex.org/W2155719068","https://openalex.org/W2156185271","https://openalex.org/W2157593764","https://openalex.org/W2157815481","https://openalex.org/W2160084442","https://openalex.org/W2161273461","https://openalex.org/W2161630640","https://openalex.org/W2162095292","https://openalex.org/W2164096594","https://openalex.org/W2164856850","https://openalex.org/W2167153439","https://openalex.org/W2169515696","https://openalex.org/W2172287485","https://openalex.org/W2402831555","https://openalex.org/W2417956508","https://openalex.org/W2469522767","https://openalex.org/W4230390154","https://openalex.org/W4241689064","https://openalex.org/W4252407890"],"related_works":["https://openalex.org/W2151607788","https://openalex.org/W2902731211","https://openalex.org/W4296036875","https://openalex.org/W4366438","https://openalex.org/W3004178518","https://openalex.org/W72217958","https://openalex.org/W2060096001","https://openalex.org/W7005536072","https://openalex.org/W3191913142","https://openalex.org/W2789146031"],"abstract_inverted_index":{"Many":[0],"biopharmaceutical":[1],"projects":[2],"require":[3],"the":[4,22,26,32,37,40,51,74,79,109,120,125,133,140,143,150,157,165,170,223,230,234,240,254,283,296,303,309],"production":[5,19,110,202,255,284,310],"of":[6,25,34,39,69,78,98,111,152,156,169,179,182,222,236,242,256,259,275,285,288,295,305,311],"recombinant":[7,17,200,289],"protein":[8,18,53,81,114,201,290],"in":[9,31,55,101,124,161,293],"a":[10,112,273],"bacterial":[11,46,126,277],"host.":[12],"Conventional":[13],"procedures":[14],"used":[15],"for":[16,189,271,282],"(RPP)":[20],"involve":[21],"rapid":[23],"synthesis":[24,168,241],"target":[27,52,80,171,185,226,260],"protein.":[28,172,227],"This":[29,84,146],"results":[30],"accumulation":[33],"unfolded":[35],"protein,":[36,261],"induction":[38,134,151],"heat":[41],"shock":[42],"stress":[43,141,215,231],"response":[44],"and":[45,58,76,115,163,177,216,313],"growth":[47,176],"arrest.":[48],"More":[49],"importantly,":[50],"accumulates":[54],"inclusion":[56],"bodies":[57],"hence":[59],"is":[60,71,233,291],"useless":[61],"to":[62,94,138,213,218,229,252],"determine":[63],"its":[64],"structure.":[65],"The":[66,269],"immediate":[67],"impact":[68],"this":[70,96,174],"that":[72,91,197,238,279],"both":[73],"yield":[75],"quality":[77],"are":[82,211,280,316],"compromised.":[83],"thesis":[85],"reports":[86],"two":[87],"generically":[88],"successful":[89],"approaches":[90],"were":[92,106,187,206,250],"developed":[93,107],"overcome":[95],"series":[97],"stress-induced":[99],"events":[100],"Escherichia":[102],"coli.":[103],"Both":[104],"strategies":[105],"during":[108,203,308],"cytoplasmic":[113],"outer":[116],"membrane":[117],"lipoproteins":[118],"using":[119],"pET":[121],"expression":[122],"system":[123],"host,":[127],"E.":[128],"coli":[129],"strain":[130],"BL21(DE3)*.":[131],"First,":[132],"protocol":[135],"was":[136],"modified":[137],"minimise":[139],"on":[142,149],"host":[144],"bacterium.":[145],"method":[147],"relies":[148],"very":[153],"low":[154],"levels":[155,221],"T7":[158,243],"RNA":[159,244],"polymerase":[160],"BL21*":[162],"thus":[164],"correspondingly":[166],"slow":[167],"Using":[173],"approach,":[175],"productivity":[178],"different":[180,247,257,286],"types":[181,258,287],"correctly":[183,224],"folded":[184,225],"proteins":[186,315],"sustained":[188],"at":[190],"least":[191],"70":[192],"h.":[193],"Secondly,":[194],"mutant":[195,306],"hosts":[196,210,249,278,307],"significantly":[198],"improve":[199],"conventional":[204],"protocols":[205],"isolated.":[207],"These":[208],"improved":[209,248,276],"resistant":[212],"IPTG-induced":[214],"continue":[217],"accumulate":[219],"high":[220],"Key":[228],"resistance":[232],"presence":[235],"mutations":[237],"downregulate":[239],"polymerase.":[245],"However,":[246],"able":[251],"enhance":[253],"such":[262],"as":[263],"those":[264],"requiring":[265],"extensive":[266],"post-translational":[267],"modification.":[268],"potential":[270],"isolating":[272],"plethora":[274],"tailored":[281],"discussed":[292],"light":[294],"challenges":[297],"faced":[298],"by":[299],"bioindustry.":[300],"Procedures":[301],"enabling":[302],"isolation":[304],"GFP-tagged":[312],"untagged":[314],"reported.":[317]},"counts_by_year":[{"year":2024,"cited_by_count":1},{"year":2018,"cited_by_count":1},{"year":2015,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}