{"id":"https://openalex.org/W8677036","doi":null,"title":"Generation of a Vancomycin-Resistant Enterococcus faecium Clinical Isolate Expressing a (FMN)-Based Fluorescent Protein","display_name":"Generation of a Vancomycin-Resistant Enterococcus faecium Clinical Isolate Expressing a (FMN)-Based Fluorescent Protein","publication_year":2014,"publication_date":"2014-09-25","ids":{"openalex":"https://openalex.org/W8677036","mag":"8677036"},"language":"en","primary_location":{"id":"mag:8677036","doi":null,"is_oa":false,"landing_page_url":"https://riunet.upv.es/bitstream/10251/40249/1/TFG%20Rafa%20Pinilla.pdf","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null},"volume":null,"type":"article","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5004296344","display_name":"Rafael Pinilla\u2010Redondo","orcid":"https://orcid.org/0000-0003-3521-460X","observed_orcids":["https://orcid.org/0000-0003-3521-460X"]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Rafael Pinilla Redondo","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5004296344"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.00014923,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10147","display_name":"Antibiotic Resistance in Bacteria","score":0.301800012588501,"subfield":{"id":"https://openalex.org/subfields/2726","display_name":"Microbiology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10147","display_name":"Antibiotic Resistance in Bacteria","score":0.301800012588501,"subfield":{"id":"https://openalex.org/subfields/2726","display_name":"Microbiology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.27709999680519104,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10195","display_name":"Antimicrobial Resistance in Staphylococcus","score":0.08699999749660492,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/vancomycin-resistant-enterococcus-faecium","display_name":"vancomycin-resistant Enterococcus faecium","score":0.9089999794960022},{"id":"https://openalex.org/keywords/fluorescent-proteins","display_name":"fluorescent proteins","score":0.5360000133514404},{"id":"https://openalex.org/keywords/vancomycin-resistance","display_name":"vancomycin resistance","score":0.6299999952316284},{"id":"https://openalex.org/keywords/multidrug-resistance","display_name":"multidrug resistance","score":0.7450000047683716},{"id":"https://openalex.org/keywords/gastrointestinal-colonization","display_name":"intestinal colonization","score":0.5630000233650208},{"id":"https://openalex.org/keywords/probiotic-bacteria","display_name":"probiotic bacteria","score":0.49000000953674316},{"id":"https://openalex.org/keywords/flow-cytometry","display_name":"flow cytometry","score":0.4659999907016754},{"id":"https://openalex.org/keywords/translational-imaging","display_name":"translational imaging","score":0.38600000739097595},{"id":"https://openalex.org/keywords/differential-gene-expression","display_name":"differential gene expression","score":0.5699999928474426}],"concepts":[{"id":"https://openalex.org/C2777677444","wikidata":"https://www.wikidata.org/wiki/Q140010","display_name":"Enterococcus faecium","level":3,"score":0.808339536190033},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.726087749004364},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6712725162506104},{"id":"https://openalex.org/C2779443120","wikidata":"https://www.wikidata.org/wiki/Q650912","display_name":"Bacteremia","level":3,"score":0.5974583625793457},{"id":"https://openalex.org/C501593827","wikidata":"https://www.wikidata.org/wiki/Q12187","display_name":"Antibiotics","level":2,"score":0.5583010911941528},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.5582202076911926},{"id":"https://openalex.org/C2778980435","wikidata":"https://www.wikidata.org/wiki/Q424027","display_name":"Vancomycin","level":4,"score":0.5021984577178955},{"id":"https://openalex.org/C539455810","wikidata":"https://www.wikidata.org/wiki/Q739734","display_name":"Gut flora","level":2,"score":0.46880701184272766},{"id":"https://openalex.org/C2779489039","wikidata":"https://www.wikidata.org/wiki/Q188121","display_name":"Staphylococcus aureus","level":3,"score":0.2748492956161499},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.2598823308944702},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.11357909440994263}],"mesh":[],"locations_count":1,"locations":[{"id":"mag:8677036","doi":null,"is_oa":false,"landing_page_url":"https://riunet.upv.es/bitstream/10251/40249/1/TFG%20Rafa%20Pinilla.pdf","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.8371999859809875}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":34,"referenced_works":["https://openalex.org/W22484262","https://openalex.org/W843658569","https://openalex.org/W1572099531","https://openalex.org/W1902000402","https://openalex.org/W1965143457","https://openalex.org/W1976667730","https://openalex.org/W1979573684","https://openalex.org/W1980229143","https://openalex.org/W1990473825","https://openalex.org/W1991383594","https://openalex.org/W1993205284","https://openalex.org/W1994243307","https://openalex.org/W2015922123","https://openalex.org/W2023055820","https://openalex.org/W2047714123","https://openalex.org/W2048469790","https://openalex.org/W2060481089","https://openalex.org/W2064577406","https://openalex.org/W2078994321","https://openalex.org/W2088284295","https://openalex.org/W2088726933","https://openalex.org/W2095040140","https://openalex.org/W2106900261","https://openalex.org/W2108876055","https://openalex.org/W2113121269","https://openalex.org/W2119884047","https://openalex.org/W2130925072","https://openalex.org/W2140886260","https://openalex.org/W2145783127","https://openalex.org/W2151921983","https://openalex.org/W2161273650","https://openalex.org/W2165211912","https://openalex.org/W2171454118","https://openalex.org/W2500020915"],"related_works":["https://openalex.org/W4407287576","https://openalex.org/W4396731062","https://openalex.org/W2301212318","https://openalex.org/W3126917598","https://openalex.org/W7166121322","https://openalex.org/W2737284717","https://openalex.org/W4400763985","https://openalex.org/W2155867629","https://openalex.org/W3006632818","https://openalex.org/W7223109210"],"abstract_inverted_index":{"Infections":[0],"caused":[1],"by":[2,65,92,268,434],"multidrug-resistant":[3],"(MDR)":[4],"bacteria,":[5],"including":[6],"Vancomycin-Resistant":[7,359],"Enterococcus":[8,360],"(VRE),":[9],"have":[10,36],"become":[11],"one":[12,40],"of":[13,18,26,32,41,46,57,61,68,104,128,143,152,214,227,242,260,270,276,295,297,310,316,322,333,346,357,381,394,450,466],"the":[14,19,33,42,55,69,88,101,105,124,144,150,169,205,225,228,240,243,253,258,261,274,308,331,343,355,374,379,391,398,404,418,436,440,448,464,467,478],"greatest":[15],"clinical":[16,362],"challenges":[17],"21":[20],"st":[21],"century.":[22],"Particularly,":[23],"VRE":[24,298,311],"strains":[25],"E.":[27,129,163,215,279,411,424,451],"faecium,":[28,452],"a":[29,218,248,277,288,293,336,358,366,444],"natural":[30],"member":[31],"gastrointestinal":[34],"consortia,":[35],"recently":[37],"emerged":[38],"as":[39,171,173,185,187,202,247,287,335],"most":[43,328],"problematic":[44],"cases":[45],"MDR":[47],"nosocomial":[48],"pathogens.":[49],"It":[50,305],"is":[51,123,165,212,231],"widely":[52],"known":[53],"that":[54,213],"establishment":[56],"high-level":[58],"intestinal":[59,110],"colonization":[60,300],"this":[62,222,347,395],"bacterium,":[63],"triggered":[64],"antibiotic":[66,141],"treatment":[67,142],"host,":[70,145],"potentially":[71],"leads":[72],"to":[73,98,114,120,167,199,209,220,234,291,325,353,389,422,447],"bacteremia,":[74],"endocarditis":[75],"and":[76,81,108,139,146,161,413,477],"surgical":[77],"wound,":[78],"urinary":[79],"tract,":[80],"devicerelated":[82],"infections":[83],"in":[84,131,149,239,378,410,459],"hospitalized":[85],"patients.":[86],"However,":[87],"unique":[89],"determinants":[90],"possessed":[91],"these":[93],"strains,":[94],"which":[95,301],"enable":[96,307],"them":[97],"benefit":[99],"from":[100,224,257],"antibiotic-induced":[102],"perturbations":[103],"gut":[106,229,244],"microbiota":[107,230,263],"host":[109],"immune":[111],"defenses,":[112],"remain":[113,302],"be":[115,121,195,200,210,265],"identified.":[116],"Essentially,":[117],"what":[118,191],"needs":[119],"revealed":[122],"differential":[125],"gene":[126,400,438],"expression":[127,207,409],"faecium":[130,164,216,256,280,299,361,412,425,473],"its":[132,178,182,327,408,415],"niche":[133],"at":[134],"different":[135],"time":[136],"points;":[137],"before":[138],"during":[140],"very":[147],"interestingly,":[148],"presence":[151],"probiotic":[153],"bacteria.":[154],"Differentially":[155],"expressed":[156],"genes":[157],"will":[158],"highlight":[159],"how":[160,174],"why":[162],"able":[166],"dominate":[168],"gut,":[170],"well":[172,186],"it":[175,284],"interacts":[176],"with":[177,403],"surroundings,":[179],"possibly":[180],"pinpointing":[181],"potential":[183],"weaknesses,":[184],"provide":[188],"hints":[189],"on":[190],"specific":[192],"properties":[193,427],"should":[194],"prerequisites":[196],"for":[197,407,414],"bacteria":[198,223],"used":[201],"probiotics.":[203],"Since":[204],"global":[206],"intended":[208],"analyzed":[211],"alone,":[217],"method":[219],"separate":[221],"rest":[226,259],"required.":[232],"Failure":[233],"do":[235],"so":[236],"would":[237,285,306],"result":[238],"study":[241,292],"microbiota\u2019s":[245],"transcriptome":[246],"whole:":[249],"metatranscriptomics.":[250],"In":[251,387],"practice,":[252],"separation":[254],"VRE.":[255],"commensal":[262],"could":[264],"carried":[266],"out":[267],"means":[269],"flow":[271],"cytometry":[272],"if":[273],"generation":[275,356],"fluorescent":[278,367,384,426],"was":[281,401,429,432,454,474],"achieved.":[282],"Additionally,":[283],"serve":[286],"great":[289],"tool":[290],"series":[294],"facets":[296],"virtually":[303],"unknown.":[304],"monitorization":[309],"infection":[312],"through":[313],"whole-body":[314],"imaging":[315],"infected":[317],"mice,":[318],"fluorescence":[319,480],"microscopy":[320],"analysis":[321],"histological":[323],"cuts":[324],"determine":[326],"predominant":[329],"locations,":[330],"use":[332],"FbFP":[334,399,437],"translational":[337],"or":[338],"transcriptional":[339],"reporter,":[340],"etc.":[341],"Consequently,":[342],"main":[344,392],"objective":[345,393],"Final":[348],"Degree":[349],"Project":[350],"has":[351],"been":[352],"attempt":[354],"isolate":[363],"(C68)":[364],"expressing":[365],"protein":[368,385],"whose":[369],"chromophore":[370],"can":[371],"form":[372],"under":[373],"anaerobic":[375],"conditions":[376],"found":[377],"guts":[380],"animals:":[382],"(FMN)-based":[383],"(FbFP).":[386],"order":[388,460],"fulfill":[390],"work,":[396],"first,":[397],"synthesized":[402],"appropriate":[405],"features":[406],"cloning.":[416],"Next,":[417],"plasmid":[419,468],"construction":[420],"aimed":[421],"confer":[423],"(pBT2-FbFP),":[428],"generated.":[430],"This":[431],"done":[433],"inserting":[435],"into":[439,456],"pBT2":[441],"plasmid.":[442],"As":[443],"previous":[445],"step":[446],"transformation":[449],"pBT2-FbFP":[453],"transformed":[455,476],"E.coli":[457],"DH5a":[458],"more":[461],"efficiently":[462],"validate":[463],"integrity":[465],"construction.":[469],"After":[470],"validation,":[471],"E":[472],"finally":[475],"corresponding":[479],"analyses":[481],"were":[482],"performed.":[483]},"counts_by_year":[],"updated_date":"2026-10-12T09:40:04.860316","created_date":"2016-06-24T00:00:00"}
