{"id":"https://openalex.org/W86695355","doi":null,"title":"Glucose-induced increase in paracellular permeability and disruption of beta-receptor signaling in retinal endothelium.","display_name":"Glucose-induced increase in paracellular permeability and disruption of beta-receptor signaling in retinal endothelium.","publication_year":1998,"publication_date":"1998-08-01","ids":{"openalex":"https://openalex.org/W86695355","mag":"86695355","pmid":"https://pubmed.ncbi.nlm.nih.gov/9699557"},"language":"en","primary_location":{"id":"pmid:9699557","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/9699557","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Investigative ophthalmology & visual science","raw_type":"Journal Article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5050891184","display_name":"Frederick R. Haselton","orcid":"https://orcid.org/0000-0003-4282-5222","observed_orcids":["https://orcid.org/0000-0003-4282-5222"]},"institutions":[{"id":"https://openalex.org/I200719446","display_name":"Vanderbilt University","ror":"https://ror.org/02vm5rt34","country_code":"US","type":"education","lineage":["https://openalex.org/I200719446"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"F R Haselton","raw_affiliation_strings":["Department of Biomedical Engineering, School of Engineering, Vanderbilt University, Nashville, Tennessee 37235, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biomedical Engineering, School of Engineering, Vanderbilt University, Nashville, Tennessee 37235, USA","institution_ids":["https://openalex.org/I200719446"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5074808473","display_name":"ELIZABETH DWORSKA","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"E J Dworska","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5111992958","display_name":"Loren H. Hoffman","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"L H Hoffman","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.608,"has_fulltext":false,"cited_by_count":18,"citation_normalized_percentile":{"value":0.65795567,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"39","issue":"9","first_page":"1676","last_page":"84"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11872","display_name":"Barrier Structure and Function Studies","score":0.5741999745368958,"subfield":{"id":"https://openalex.org/subfields/2808","display_name":"Neurology"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11872","display_name":"Barrier Structure and Function Studies","score":0.5741999745368958,"subfield":{"id":"https://openalex.org/subfields/2808","display_name":"Neurology"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10170","display_name":"Retinal Diseases and Treatments","score":0.40799999237060547,"subfield":{"id":"https://openalex.org/subfields/2731","display_name":"Ophthalmology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11480","display_name":"Connexins and lens biology","score":0.003000000026077032,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/high-glucose","display_name":"high glucose","score":0.8289999961853027},{"id":"https://openalex.org/keywords/retinal-endothelium","display_name":"retinal endothelium","score":0.871999979019165},{"id":"https://openalex.org/keywords/paracellular-permeability","display_name":"paracellular permeability","score":0.8880000114440918},{"id":"https://openalex.org/keywords/endothelial-barrier","display_name":"endothelial barrier","score":0.8109999895095825},{"id":"https://openalex.org/keywords/beta-adrenergic-signaling","display_name":"beta-adrenergic signaling","score":0.8989999890327454},{"id":"https://openalex.org/keywords/isoproterenol","display_name":"isoproterenol","score":0.9940000176429749},{"id":"https://openalex.org/keywords/diabetic-retinopathy","display_name":"diabetic retinopathy","score":0.9300000071525574},{"id":"https://openalex.org/keywords/retinal-microvasculature","display_name":"retinal microvasculature","score":0.8960000276565552},{"id":"https://openalex.org/keywords/endothelial-permeability","display_name":"endothelial permeability","score":0.5289999842643738}],"concepts":[{"id":"https://openalex.org/C120882062","wikidata":"https://www.wikidata.org/wiki/Q28352","display_name":"Permeability (electromagnetism)","level":3,"score":0.6546886563301086},{"id":"https://openalex.org/C60903277","wikidata":"https://www.wikidata.org/wiki/Q2051926","display_name":"Paracellular transport","level":4,"score":0.6360630989074707},{"id":"https://openalex.org/C2778371105","wikidata":"https://www.wikidata.org/wiki/Q1622103","display_name":"Blood\u2013retinal barrier","level":4,"score":0.5864531993865967},{"id":"https://openalex.org/C2780827179","wikidata":"https://www.wikidata.org/wiki/Q422001","display_name":"Retinal","level":2,"score":0.5504178404808044},{"id":"https://openalex.org/C123012128","wikidata":"https://www.wikidata.org/wiki/Q5376430","display_name":"Endothelial stem cell","level":3,"score":0.5171996355056763},{"id":"https://openalex.org/C176999471","wikidata":"https://www.wikidata.org/wiki/Q6839314","display_name":"Microcarrier","level":3,"score":0.5131500363349915},{"id":"https://openalex.org/C113045295","wikidata":"https://www.wikidata.org/wiki/Q7916465","display_name":"Vascular permeability","level":2,"score":0.5061132311820984},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4825655519962311},{"id":"https://openalex.org/C134018914","wikidata":"https://www.wikidata.org/wiki/Q162606","display_name":"Endocrinology","level":1,"score":0.4503461718559265},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.44268661737442017},{"id":"https://openalex.org/C2776992346","wikidata":"https://www.wikidata.org/wiki/Q111140","display_name":"Endothelium","level":2,"score":0.4289228618144989},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.4288644790649414},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.30867093801498413},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.23176974058151245},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.19688713550567627},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.19139569997787476},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.13220283389091492},{"id":"https://openalex.org/C555293320","wikidata":"https://www.wikidata.org/wiki/Q12206","display_name":"Diabetes mellitus","level":2,"score":0.07863131165504456},{"id":"https://openalex.org/C41625074","wikidata":"https://www.wikidata.org/wiki/Q176088","display_name":"Membrane","level":2,"score":0.0},{"id":"https://openalex.org/C2779829184","wikidata":"https://www.wikidata.org/wiki/Q631361","display_name":"Diabetic retinopathy","level":3,"score":0.0}],"mesh":[{"descriptor_ui":"D000318","descriptor_name":"Adrenergic beta-Agonists","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002199","descriptor_name":"Capillary Permeability","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":true},{"descriptor_ui":"D002417","descriptor_name":"Cattle","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002478","descriptor_name":"Cells, Cultured","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004730","descriptor_name":"Endothelium, Vascular","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":true},{"descriptor_ui":"D004730","descriptor_name":"Endothelium, Vascular","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D004730","descriptor_name":"Endothelium, Vascular","qualifier_ui":"Q000648","qualifier_name":"ultrastructure","is_major_topic":false},{"descriptor_ui":"D005947","descriptor_name":"Glucose","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true},{"descriptor_ui":"D007545","descriptor_name":"Isoproterenol","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D008855","descriptor_name":"Microscopy, Electron, Scanning","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011943","descriptor_name":"Receptors, Adrenergic, beta","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D012171","descriptor_name":"Retinal Vessels","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":true},{"descriptor_ui":"D012171","descriptor_name":"Retinal Vessels","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D012171","descriptor_name":"Retinal Vessels","qualifier_ui":"Q000648","qualifier_name":"ultrastructure","is_major_topic":false},{"descriptor_ui":"D014805","descriptor_name":"Vitamin B 12","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D019793","descriptor_name":"Fluorescein","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false}],"locations_count":2,"locations":[{"id":"pmid:9699557","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/9699557","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Investigative ophthalmology & visual science","raw_type":"Journal Article"},{"id":"mag:86695355","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/9699557","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.9391000270843506,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G4698671575","display_name":"REGULATION OF RETINAL MICROVESSEL PERMEABILITY IN VITRO","funder_award_id":"R01EY010086","funder_id":"https://openalex.org/F4320337350","funder_display_name":"National Eye Institute"}],"funders":[{"id":"https://openalex.org/F4320337350","display_name":"National Eye Institute","ror":"https://ror.org/03wkg3b53"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W54412367","https://openalex.org/W2903011421","https://openalex.org/W2031504244","https://openalex.org/W2003248465","https://openalex.org/W1979312493","https://openalex.org/W7047587574","https://openalex.org/W6991518072","https://openalex.org/W2158185799","https://openalex.org/W4242660529","https://openalex.org/W2162230859"],"abstract_inverted_index":{"PURPOSE:":[0],"To":[1],"examine":[2],"the":[3,19,26,60,67,130,174,197,202,206,268,274,302],"effects":[4,74,104],"of":[5,18,25,40,75,109,118,205,218,237,259,288],"high":[6,96],"glucose":[7,76,112,119,147,176,187,226,257],"concentrations":[8],"on":[9,46,120,129],"retinal":[10,20,42,152,219,269,297,303],"endothelial":[11,27,43,153,220,270],"permeability":[12,24,54,62,81,131,156,166,194,203,242,290],"in":[13,15,34,88,164,173,193,213,223,246,254,284],"an":[14,86,162],"vitro":[16],"model":[17,280],"microvasculature.":[21],"METHODS:":[22],"The":[23,116],"barrier":[28,73,271],"to":[29,95,113,144,184,235,276],"small":[30],"solutes":[31],"was":[32,123,167,208],"measured":[33],"a":[35,191],"chromatographic":[36],"cell":[37,52,154,248],"column":[38],"consisting":[39],"bovine":[41],"cells":[44],"cultured":[45,222,253],"porous":[47],"fibronectin-coated":[48],"microcarriers.":[49],"In":[50],"each":[51],"column,":[53],"changes":[55],"were":[56,77,105],"evaluated":[57],"by":[58,79,107,125,134,196,210],"comparing":[59],"treatment":[61],"response":[63,275],"over":[64],"time":[65,180],"with":[66,229,292],"initial":[68],"baseline":[69,92,238,260],"permeability.":[70],"Short-term":[71],"(2-hour)":[72],"examined":[78],"measuring":[80,126],"at":[82],"15-minute":[83],"intervals":[84],"after":[85,169],"increase":[87,140,163],"perfusate":[89],"concentration":[90,148],"from":[91,141,251],"(5.5":[93],"mM)":[94,98],"(25":[97],"glucose.":[99,216],"Long-term":[100],"(to":[101],"57":[102],"days)":[103],"tested":[106,124],"addition":[108],"25":[110,145,185,214,255],"mM":[111,143,146,186,215,225,256],"microcarrier":[114],"cultures.":[115],"effect":[117,128,204],"beta-receptor":[121,198],"signaling":[122],"its":[127],"decrease":[132,192],"produced":[133],"1":[135,230],"microM":[136,231],"isoproterenol.":[137,200],"RESULTS:":[138],"An":[139],"5.5":[142,224],"did":[149,188,243],"not":[150,189,244],"change":[151,245],"monolayer":[155,165],"(n=6)":[157],"during":[158],"2":[159],"hours.":[160],"However,":[161,201,241],"observed":[168],"19":[170],"days":[171],"(n=8)":[172],"25-mM":[175],"culture.":[177],"Paralleling":[178],"this":[179],"course,":[181],"short-term":[182],"exposure":[183],"prevent":[190],"triggered":[195],"agonist":[199,207],"blocked":[209],"long-term":[211],"culture":[212,266],"Permeability":[217],"monolayers":[221],"and":[227,272],"treated":[228],"isoproterenol":[232],"decreased":[233],"significantly":[234],"0.71+/-0.06":[236],"(n=4;":[239],"mean+/-SEM).":[240,263],"parallel":[247],"columns":[249],"made":[250],"microcarriers":[252],"(0.97+/-0.2":[258],"permeability;":[261],"n=4;":[262],"CONCLUSIONS:":[264],"High-glucose":[265],"decreases":[267],"blocks":[273],"beta-adrenergic":[277],"receptors.":[278],"This":[279],"may":[281],"prove":[282],"valuable":[283],"exploring":[285],"other":[286,296],"hypotheses":[287],"increased":[289],"associated":[291],"diabetic":[293],"retinopathy":[294],"or":[295],"diseases":[298],"that":[299],"break":[300],"down":[301],"vascular":[304],"barrier.":[305]},"counts_by_year":[{"year":2019,"cited_by_count":3},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":1},{"year":2013,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}