{"id":"https://openalex.org/W86600580","doi":"https://doi.org/10.3760/cma.j.issn.2095-4352.2014.05.002","title":"[The use of 16S rDNA sequencing in species diversity analysis for sputum of patients with ventilator-associated pneumonia].","display_name":"[The use of 16S rDNA sequencing in species diversity analysis for sputum of patients with ventilator-associated pneumonia].","publication_year":2014,"publication_date":"2014-05-01","ids":{"openalex":"https://openalex.org/W86600580","doi":"https://doi.org/10.3760/cma.j.issn.2095-4352.2014.05.002","mag":"86600580","pmid":"https://pubmed.ncbi.nlm.nih.gov/24809255"},"language":"zh","primary_location":{"id":"pmid:24809255","doi":"https://doi.org/10.3760/cma.j.issn.2095-4352.2014.05.002","is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/24809255","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Zhonghua wei zhong bing ji jiu yi xue","raw_type":"English Abstract"},"volume":null,"type":"article","indexed_in":["pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5074963243","display_name":"Xiaojun Yang","orcid":"https://orcid.org/0000-0003-3280-7725","observed_orcids":["https://orcid.org/0000-0003-3280-7725"]},"institutions":[{"id":"https://openalex.org/I4210127460","display_name":"Ningxia Medical University","ror":"https://ror.org/02h8a1848","country_code":"CN","type":"education","lineage":["https://openalex.org/I4210127460"]},{"id":"https://openalex.org/I4210139449","display_name":"Ningxia Medical University General Hospital","ror":"https://ror.org/049dkqr57","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210139449"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Xiaojun Yang","raw_affiliation_strings":["Department of Critical Care Medicine, General Hospital of Ningxia Medical University, Yinchun 750004, Ningxia, China. Corresponding author: Yang Xiaojun, Email: yxjicu@163.com"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Critical Care Medicine, General Hospital of Ningxia Medical University, Yinchun 750004, Ningxia, China. Corresponding author: Yang Xiaojun, Email: yxjicu@163.com","institution_ids":["https://openalex.org/I4210127460","https://openalex.org/I4210139449"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5083195901","display_name":"Xiaohong Wang","orcid":"https://orcid.org/0000-0002-0980-6575","observed_orcids":["https://orcid.org/0000-0002-0980-6575"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Xiaohong Wang","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100525827","display_name":"Zhijuan Liang","orcid":"https://orcid.org/0000-0002-3760-740X","observed_orcids":["https://orcid.org/0000-0002-3760-740X"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Zhijuan Liang","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100718903","display_name":"Xiaoya Zhang","orcid":"https://orcid.org/0000-0003-3281-4335","observed_orcids":["https://orcid.org/0000-0003-3281-4335"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Xiaoya Zhang","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5115695201","display_name":"Yanbo Wang","orcid":"https://orcid.org/0000-0003-3428-4585","observed_orcids":["https://orcid.org/0000-0003-3428-4585","https://orcid.org/0009-0006-4520-6987"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Yanbo Wang","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5101624351","display_name":"Zhenhai Wang","orcid":"https://orcid.org/0000-0002-3677-0985","observed_orcids":["https://orcid.org/0000-0002-3677-0985"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Zhenhai Wang","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.2681,"has_fulltext":false,"cited_by_count":2,"citation_normalized_percentile":{"value":0.6457578,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":94},"biblio":{"volume":"26","issue":"5","first_page":"294","last_page":"9"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11681","display_name":"Nosocomial Infections in ICU","score":0.7666000127792358,"subfield":{"id":"https://openalex.org/subfields/2706","display_name":"Critical Care and Intensive Care Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T11681","display_name":"Nosocomial Infections in ICU","score":0.7666000127792358,"subfield":{"id":"https://openalex.org/subfields/2706","display_name":"Critical Care and Intensive Care Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T12167","display_name":"Bacterial Identification and Susceptibility Testing","score":0.19059999287128448,"subfield":{"id":"https://openalex.org/subfields/1308","display_name":"Clinical Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10015","display_name":"Genomics and Phylogenetic Studies","score":0.003800000064074993,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/ventilator-associated-pneumonia","display_name":"ventilator-associated pneumonia","score":0.9980000257492065},{"id":"https://openalex.org/keywords/sputum-microbiota","display_name":"sputum microbiota","score":0.7590000033378601},{"id":"https://openalex.org/keywords/16s-rrna-gene-sequencing","display_name":"16S rRNA gene sequencing","score":0.8989999890327454},{"id":"https://openalex.org/keywords/bacterial-species-diversity","display_name":"bacterial species diversity","score":0.8659999966621399},{"id":"https://openalex.org/keywords/etiological-diagnosis","display_name":"etiological diagnosis","score":0.7720000147819519},{"id":"https://openalex.org/keywords/bronchoalveolar-lavage","display_name":"bronchoalveolar lavage","score":0.8489999771118164},{"id":"https://openalex.org/keywords/streptococcus","display_name":"Streptococcus","score":0.8100000023841858},{"id":"https://openalex.org/keywords/acinetobacter","display_name":"Acinetobacter","score":0.9739999771118164},{"id":"https://openalex.org/keywords/pseudomonas","display_name":"Pseudomonas","score":0.8190000057220459},{"id":"https://openalex.org/keywords/klebsiella","display_name":"Klebsiella","score":0.8640000224113464},{"id":"https://openalex.org/keywords/bacterial-culture-comparison","display_name":"bacterial culture comparison","score":0.6290000081062317},{"id":"https://openalex.org/keywords/alpha-diversity","display_name":"alpha diversity","score":0.7889999747276306},{"id":"https://openalex.org/keywords/rarefaction-curves","display_name":"rarefaction curves","score":0.46000000834465027},{"id":"https://openalex.org/keywords/metagenomics","display_name":"metagenomics","score":0.46000000834465027},{"id":"https://openalex.org/keywords/nosocomial-infections","display_name":"nosocomial infections","score":0.4790000021457672},{"id":"https://openalex.org/keywords/critical-care","display_name":"intensive care","score":0.39100000262260437}],"concepts":[{"id":"https://openalex.org/C2776301714","wikidata":"https://www.wikidata.org/wiki/Q259346","display_name":"Sputum","level":3,"score":0.824931800365448},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.5718768239021301},{"id":"https://openalex.org/C42062724","wikidata":"https://www.wikidata.org/wiki/Q1209205","display_name":"16S ribosomal RNA","level":3,"score":0.5010647773742676},{"id":"https://openalex.org/C2777961210","wikidata":"https://www.wikidata.org/wiki/Q927148","display_name":"Bronchoalveolar lavage","level":3,"score":0.5002763271331787},{"id":"https://openalex.org/C2777695942","wikidata":"https://www.wikidata.org/wiki/Q149075","display_name":"Firmicutes","level":4,"score":0.48167622089385986},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4660642743110657},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.43488311767578125},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.4310043156147003},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.21772369742393494},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.20239868760108948},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.17295849323272705},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.1349506676197052},{"id":"https://openalex.org/C2781069245","wikidata":"https://www.wikidata.org/wiki/Q12204","display_name":"Tuberculosis","level":2,"score":0.12518680095672607},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.11639192700386047},{"id":"https://openalex.org/C2777714996","wikidata":"https://www.wikidata.org/wiki/Q7886","display_name":"Lung","level":2,"score":0.08829286694526672}],"mesh":[{"descriptor_ui":"D000328","descriptor_name":"Adult","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000368","descriptor_name":"Aged","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000369","descriptor_name":"Aged, 80 and over","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001419","descriptor_name":"Bacteria","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D001431","descriptor_name":"Bacteriological Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004269","descriptor_name":"DNA, Bacterial","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D005260","descriptor_name":"Female","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008297","descriptor_name":"Male","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008875","descriptor_name":"Middle Aged","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012336","descriptor_name":"RNA, Ribosomal, 16S","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D013183","descriptor_name":"Sputum","qualifier_ui":"Q000382","qualifier_name":"microbiology","is_major_topic":true},{"descriptor_ui":"D017422","descriptor_name":"Sequence Analysis, DNA","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D053717","descriptor_name":"Pneumonia, Ventilator-Associated","qualifier_ui":"Q000382","qualifier_name":"microbiology","is_major_topic":true}],"locations_count":2,"locations":[{"id":"pmid:24809255","doi":"https://doi.org/10.3760/cma.j.issn.2095-4352.2014.05.002","is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/24809255","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Zhonghua wei zhong bing ji jiu yi xue","raw_type":"English Abstract"},{"id":"mag:86600580","doi":"https://doi.org/10.3760/cma.j.issn.2095-4352.2014.05.002","is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/24809255","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.9476000070571899}],"study_designs":[{"display_name":"Observational Study","id":"https://openalex.org/study-designs/observational-study"}],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W1555265423","https://openalex.org/W2412462941","https://openalex.org/W4284888786","https://openalex.org/W3138442850","https://openalex.org/W6939911842","https://openalex.org/W2046864303","https://openalex.org/W7211225783","https://openalex.org/W2035548107","https://openalex.org/W4410273718","https://openalex.org/W6920961785"],"abstract_inverted_index":{"OBJECTIVE:":[0],"To":[1],"study":[2],"the":[3,26,49,61,98,101,125,247],"species":[4,188,205,249,298],"and":[5,23,53,90,103,130,184,275,370,382],"amount":[6],"of":[7,11,32,48,112,124,154,176,219,313,355,404,440],"bacteria":[8,104,294,315,372,384,401],"in":[9,118,165,186,402,424],"sputum":[10,37,64,119,144,174,215,221,403],"patients":[12,43,127,177],"with":[13,44,83,128,178,223,408,414],"ventilator-associated":[14],"pneumonia":[15],"(VAP)":[16],"by":[17,55,210,225,292,302],"using":[18,95,146,226],"16S":[19,84,147,227,394,427],"rDNA":[20,85,148,228,395,428],"sequencing":[21,75,88,91,102,135,158,211,281,306,369,396,419,430],"analysis,":[22],"to":[24,161,349],"explore":[25],"new":[27,435],"method":[28,436],"for":[29,68,134,353,374,437],"etiologic":[30],"diagnosis":[31,439],"VAP.":[33,45,441],"METHODS:":[34],"Bronchoalveolar":[35],"lavage":[36],"samples":[38,50,82,175,222],"were":[39,51,66,93,106,116,132,140,203,207,300,366,406],"collected":[40],"from":[41,121,142,212],"31":[42,126],"Bacterial":[46],"DNA":[47,114],"extracted":[52],"identified":[54,301],"polymerase":[56],"chain":[57],"reaction":[58],"(PCR).":[59],"At":[60],"same":[62],"time,":[63],"specimens":[65,120,145],"processed":[67],"routine":[69,293,415],"bacterial":[70,187,416],"culture.":[71,295],"The":[72],"high":[73],"flux":[74],"experiment":[76],"was":[77,159,244,347],"conducted":[78],"on":[79],"PCR":[80],"positive":[81,309,345,351,422],"macro":[86],"genome":[87],"technology,":[89],"results":[92,99],"analyzed":[94],"bioinformatics,":[96],"then":[97],"between":[100,368],"culture":[105,316,354,373],"compared.":[107],"RESULTS:":[108],"(1)":[109],"550":[110],"bp":[111],"specific":[113],"sequences":[115,139,229],"amplified":[117],"27":[122,167,220],"cases":[123],"VAP,":[129],"they":[131],"used":[133],"analysis.":[136],"103":[137],"856":[138],"obtained":[141],"those":[143],"sequencing,":[149],"yielding":[150],"approximately":[151],"39":[152],"Mb":[153],"raw":[155],"data.":[156],"Tag":[157],"able":[160],"inform":[162],"genus":[163,239],"level":[164],"all":[166],"samples.":[168,216],"(2)":[169],"Alpha-diversity":[170],"analysis":[171,199,397],"showed":[172,200],"that":[173,201,206,246,283,312,399],"VAP":[179,214,224,405],"had":[180,420],"significantly":[181],"higher":[182,308,421],"variability":[183],"richness":[185],"(Shannon":[189],"index":[190,194],"values":[191,195],"1.20,":[192],"Simpson":[193],"0.48).":[196],"Rarefaction":[197],"curve":[198],"there":[202],"more":[204],"not":[208,289],"detected":[209,291],"some":[213],"(3)":[217],"Analysis":[218],"yielded":[230,307],"four":[231],"phyla:":[232],"namely":[233],"Acitinobacteria,":[234],"Bacteroidetes,":[235],"Firmicutes,":[236],"Proteobacteria.":[237],"With":[238],"as":[240],"a":[241,434],"classification,":[242],"it":[243],"found":[245,348],"dominant":[248],"included":[250],"Streptococcus":[251],"88.9%":[252,318],"(24/27),":[253],"Limnohabitans":[254],"77.8%":[255],"(21/27),":[256],"Acinetobacter":[257],"70.4%":[258,330],"(19/27),":[259],"Sphingomonas":[260,287],"63.0%":[261,264],"(17/27),":[262,265],"Prevotella":[263],"Klebsiella":[266],"55.6%":[267,270,273,277,324,336],"(15/27),":[268,271,274],"Pseudomonas":[269,356],"Aquabacterium":[272],"Corynebacterium":[276],"(15/27).":[278],"(4)":[279],"Pyrophosphate":[280],"discovered":[282],"Prevotella,":[284],"Limnohabitans,":[285],"Aquabacterium,":[286],"might":[288],"be":[290],"Among":[296],"seven":[297],"which":[299],"both":[303,391],"methods,":[304],"pyrophosphate":[305,418],"rate":[310,346,352,423],"than":[311],"ordinary":[314,371],"[Streptococcus:":[317],"(24/27)":[319],"vs.":[320,326,332,338,359,379,388],"18.5%":[321,327],"(5/27),":[322,328],"Klebsiella:":[323],"(15/27)":[325,337,358],"Acinetobacter:":[329],"(19/27)":[331],"37.0%":[333],"(10/27),":[334],"Corynebacterium:":[335],"7.4%":[339],"(2/27),":[340],"P<0.05":[341],"or":[342],"P<0.01].":[343],"Sequencing":[344],"increase":[350],"[55.6%":[357],"25.9%":[360],"(7/27),":[361],"P=0.050].":[362],"No":[363],"significant":[364],"differences":[365],"observed":[367],"detection":[375],"Staphylococcus":[376],"[7.4%":[377],"(2/27)":[378],"11.1%":[380],"(3/27)]":[381],"Neisseria":[383],"genera":[385],"[18.5%":[386],"(5/27)":[387],"3.7%":[389],"(1/27),":[390],"P>0.05].":[392],"CONCLUSIONS:":[393],"confirmed":[398],"pathogenic":[400],"complicated":[407],"multiple":[409],"drug":[410],"resistant":[411],"strains.":[412],"Compared":[413],"culture,":[417],"detecting":[425],"pathogens.":[426],"gene":[429],"technology":[431],"may":[432],"become":[433],"etiological":[438]},"counts_by_year":[{"year":2023,"cited_by_count":1},{"year":2014,"cited_by_count":1}],"updated_date":"2026-11-02T09:45:03.842371","created_date":"2016-06-24T00:00:00"}
