{"id":"https://openalex.org/W86048632","doi":"https://doi.org/10.2144/000114104","title":"Microsatellite Marker Discovery using Single Molecule Real-Time Circular Consensus Sequencing on the Pacific Biosciences RS","display_name":"Microsatellite Marker Discovery using Single Molecule Real-Time Circular Consensus Sequencing on the Pacific Biosciences RS","publication_year":2013,"publication_date":"2013-11-01","ids":{"openalex":"https://openalex.org/W86048632","doi":"https://doi.org/10.2144/000114104","mag":"86048632","pmid":"https://pubmed.ncbi.nlm.nih.gov/24215640"},"language":"en","primary_location":{"id":"doi:10.2144/000114104","doi":"https://doi.org/10.2144/000114104","is_oa":true,"landing_page_url":"https://doi.org/10.2144/000114104","pdf_url":null,"source":{"id":"https://openalex.org/S79990748","display_name":"BioTechniques","issn_l":"0736-6205","issn":["0736-6205","1940-9818"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","jufo-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320316","host_organization_name":"Future Science Ltd","host_organization_lineage":["https://openalex.org/P4310320316"],"host_organization_lineage_names":["Future Science Ltd"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BioTechniques","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://doi.org/10.2144/000114104","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5037640562","display_name":"Markus A. Grohme","orcid":"https://orcid.org/0000-0002-4250-5600","observed_orcids":["https://orcid.org/0000-0002-4250-5600"]},"institutions":[{"id":"https://openalex.org/I38517508","display_name":"Technical University of Applied Sciences Wildau","ror":"https://ror.org/00k62pe27","country_code":"DE","type":"education","lineage":["https://openalex.org/I38517508"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Markus A. Grohme","raw_affiliation_strings":["Molecular Biotechnology and Functional Genomics, Technical University of Applied Sciences Wildau, Wildau, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Molecular Biotechnology and Functional Genomics, Technical University of Applied Sciences Wildau, Wildau, Germany","institution_ids":["https://openalex.org/I38517508"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5044570958","display_name":"Roberto Carlos Frias-Soler","orcid":"https://orcid.org/0000-0003-0942-9463","observed_orcids":["https://orcid.org/0000-0003-0942-9463"]},"institutions":[{"id":"https://openalex.org/I223822909","display_name":"Heidelberg University","ror":"https://ror.org/038t36y30","country_code":"DE","type":"education","lineage":["https://openalex.org/I223822909"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Roberto Frias Soler","raw_affiliation_strings":["Department of Biology, Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, Heidelberg, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biology, Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, Heidelberg, Germany","institution_ids":["https://openalex.org/I223822909"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5034011528","display_name":"Micha\u00ebl Wink","orcid":"https://orcid.org/0000-0002-7875-4510","observed_orcids":["https://orcid.org/0000-0002-7875-4510"]},"institutions":[{"id":"https://openalex.org/I223822909","display_name":"Heidelberg University","ror":"https://ror.org/038t36y30","country_code":"DE","type":"education","lineage":["https://openalex.org/I223822909"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Michael Wink","raw_affiliation_strings":["Department of Biology, Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, Heidelberg, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biology, Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, Heidelberg, Germany","institution_ids":["https://openalex.org/I223822909"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5026608305","display_name":"Marcus Frohme","orcid":"https://orcid.org/0000-0001-9063-2682","observed_orcids":["https://orcid.org/0000-0001-9063-2682"]},"institutions":[{"id":"https://openalex.org/I38517508","display_name":"Technical University of Applied Sciences Wildau","ror":"https://ror.org/00k62pe27","country_code":"DE","type":"education","lineage":["https://openalex.org/I38517508"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Marcus Frohme","raw_affiliation_strings":["Molecular Biotechnology and Functional Genomics,Technical University of Applied Sciences Wildau,\rWildau,\rGermany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Molecular Biotechnology and Functional Genomics,Technical University of Applied Sciences Wildau,\rWildau,\rGermany","institution_ids":["https://openalex.org/I38517508"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":1415,"currency":"USD","value_usd":1415},"apc_paid":{"value":1415,"currency":"USD","value_usd":1415},"fwci":1.9739,"has_fulltext":false,"cited_by_count":28,"citation_normalized_percentile":{"value":0.85605129,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":98},"biblio":{"volume":"55","issue":"5","first_page":"253","last_page":"256"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10012","display_name":"Genetic diversity and population structure","score":0.8403000235557556,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10012","display_name":"Genetic diversity and population structure","score":0.8403000235557556,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10015","display_name":"Genomics and Phylogenetic Studies","score":0.13590000569820404,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11468","display_name":"Genetic Mapping and Diversity in Plants and Animals","score":0.008799999952316284,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/single-molecule-real-time-sequencing","display_name":"single-molecule real-time sequencing","score":0.8679999709129333},{"id":"https://openalex.org/keywords/circular-consensus-sequencing","display_name":"circular consensus sequencing","score":0.8199999928474426},{"id":"https://openalex.org/keywords/greater-white-fronted-goose","display_name":"white-fronted goose","score":0.996999979019165},{"id":"https://openalex.org/keywords/primer-design","display_name":"primer design","score":0.7960000038146973},{"id":"https://openalex.org/keywords/non-model-organisms","display_name":"non-model organisms","score":0.6940000057220459}],"concepts":[{"id":"https://openalex.org/C61320498","wikidata":"https://www.wikidata.org/wiki/Q265193","display_name":"Microsatellite","level":4,"score":0.8562738299369812},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7093485593795776},{"id":"https://openalex.org/C101985253","wikidata":"https://www.wikidata.org/wiki/Q1073526","display_name":"Shotgun sequencing","level":4,"score":0.5997582674026489},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.5579527616500854},{"id":"https://openalex.org/C2777563447","wikidata":"https://www.wikidata.org/wiki/Q7243397","display_name":"Primer (cosmetics)","level":2,"score":0.5475297570228577},{"id":"https://openalex.org/C141231307","wikidata":"https://www.wikidata.org/wiki/Q7020","display_name":"Genome","level":3,"score":0.5272994041442871},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.5244420170783997},{"id":"https://openalex.org/C51679486","wikidata":"https://www.wikidata.org/wiki/Q380546","display_name":"DNA sequencing","level":3,"score":0.48567235469818115},{"id":"https://openalex.org/C2908647359","wikidata":"https://www.wikidata.org/wiki/Q2625603","display_name":"Population","level":2,"score":0.4346855580806732},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.1124144196510315},{"id":"https://openalex.org/C180754005","wikidata":"https://www.wikidata.org/wiki/Q80726","display_name":"Allele","level":3,"score":0.11033833026885986},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.09746012091636658},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0},{"id":"https://openalex.org/C149923435","wikidata":"https://www.wikidata.org/wiki/Q37732","display_name":"Demography","level":1,"score":0.0},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.0},{"id":"https://openalex.org/C144024400","wikidata":"https://www.wikidata.org/wiki/Q21201","display_name":"Sociology","level":0,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D005777","descriptor_name":"Geese","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D016678","descriptor_name":"Genome","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017422","descriptor_name":"Sequence Analysis, DNA","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D018895","descriptor_name":"Microsatellite Repeats","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D059014","descriptor_name":"High-Throughput Nucleotide Sequencing","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.2144/000114104","doi":"https://doi.org/10.2144/000114104","is_oa":true,"landing_page_url":"https://doi.org/10.2144/000114104","pdf_url":null,"source":{"id":"https://openalex.org/S79990748","display_name":"BioTechniques","issn_l":"0736-6205","issn":["0736-6205","1940-9818"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","jufo-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320316","host_organization_name":"Future Science Ltd","host_organization_lineage":["https://openalex.org/P4310320316"],"host_organization_lineage_names":["Future Science Ltd"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BioTechniques","raw_type":"journal-article"},{"id":"pmid:24215640","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/24215640","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BioTechniques","raw_type":"Journal Article"},{"id":"pmh:oai:doaj.org/article:2c7332bb007546568454dd5197d14e5f","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/2c7332bb007546568454dd5197d14e5f","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"BioTechniques, Vol 55, Iss 5, Pp 253-256 (2013)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.2144/000114104","doi":"https://doi.org/10.2144/000114104","is_oa":true,"landing_page_url":"https://doi.org/10.2144/000114104","pdf_url":null,"source":{"id":"https://openalex.org/S79990748","display_name":"BioTechniques","issn_l":"0736-6205","issn":["0736-6205","1940-9818"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","jufo-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320316","host_organization_name":"Future Science Ltd","host_organization_lineage":["https://openalex.org/P4310320316"],"host_organization_lineage_names":["Future Science Ltd"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BioTechniques","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320322744","display_name":"Brandenburger Staatsministerium f\u00fcr Wissenschaft, Forschung und Kultur","ror":"https://ror.org/02vcjcj30"},{"id":"https://openalex.org/F4320335322","display_name":"European Regional Development Fund","ror":"https://ror.org/00k4n6c32"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":20,"referenced_works":["https://openalex.org/W1583136020","https://openalex.org/W1969815265","https://openalex.org/W1980355960","https://openalex.org/W2003512348","https://openalex.org/W2047417387","https://openalex.org/W2080066443","https://openalex.org/W2087464200","https://openalex.org/W2103441770","https://openalex.org/W2108800379","https://openalex.org/W2121729186","https://openalex.org/W2132341951","https://openalex.org/W2134739162","https://openalex.org/W2144924760","https://openalex.org/W2160225487","https://openalex.org/W2162055898","https://openalex.org/W2170906656","https://openalex.org/W4247053599","https://openalex.org/W7097061496","https://openalex.org/W7098838276","https://openalex.org/W7099585975"],"related_works":["https://openalex.org/W2003512348","https://openalex.org/W1991015639","https://openalex.org/W6948825791","https://openalex.org/W4254158236","https://openalex.org/W2145036794","https://openalex.org/W1999352554","https://openalex.org/W4294558813","https://openalex.org/W2134739162","https://openalex.org/W2136402682","https://openalex.org/W2024824486"],"abstract_inverted_index":{"Microsatellite":[0],"sequences":[1,87],"are":[2],"important":[3],"markers":[4],"for":[5,95,112],"population":[6],"genetics":[7],"studies.":[8],"In":[9],"the":[10,12,23,54,75],"past,":[11],"development":[13],"of":[14,25,33,77],"adequate":[15],"microsatellite":[16,43,63,113],"primers":[17],"has":[18,36],"been":[19,37],"cumbersome.":[20],"However":[21],"with":[22,99],"advent":[24],"next-generation":[26],"sequencing":[27,70,106],"technologies,":[28],"marker":[29],"identification":[30],"in":[31,65],"genomes":[32],"non-model":[34],"species":[35],"greatly":[38],"simplified.":[39],"Here":[40],"we":[41,60],"describe":[42],"discovery":[44,114],"on":[45],"a":[46,66,100],"Pacific":[47],"Biosciences":[48],"single":[49,67],"molecule":[50],"real-time":[51],"sequencer.":[52],"For":[53],"Greater":[55],"White-fronted":[56],"Goose":[57],"(Anser":[58],"albifrons),":[59],"identified":[61],"316":[62],"loci":[64],"genome":[68],"shotgun":[69],"experiment.":[71],"We":[72],"found":[73],"that":[74],"capability":[76],"handling":[78],"large":[79],"insert":[80],"sizes":[81],"and":[82,115],"high":[83],"quality":[84],"circular":[85],"consensus":[86],"provides":[88],"an":[89,108],"advantage":[90],"over":[91],"short":[92],"read":[93],"technologies":[94],"primer":[96,118],"design.":[97,119],"Combined":[98],"straightforward":[101],"amplification-free":[102],"library":[103],"preparation,":[104],"PacBio":[105],"is":[107],"economically":[109],"viable":[110],"alternative":[111],"subsequent":[116],"PCR":[117]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2023,"cited_by_count":2},{"year":2022,"cited_by_count":3},{"year":2021,"cited_by_count":2},{"year":2020,"cited_by_count":1},{"year":2019,"cited_by_count":4},{"year":2018,"cited_by_count":3},{"year":2016,"cited_by_count":5},{"year":2015,"cited_by_count":3},{"year":2014,"cited_by_count":4}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}
