{"id":"https://openalex.org/W847718385","doi":null,"title":"The Development of Mass Spectrometry Based Approaches to Monitor Protease Activity in Biological Fluids","display_name":"The Development of Mass Spectrometry Based Approaches to Monitor Protease Activity in Biological Fluids","publication_year":2012,"publication_date":"2012-12-01","ids":{"openalex":"https://openalex.org/W847718385","mag":"847718385"},"language":"en","primary_location":{"id":"pmh:oai:pure.atira.dk:openaire_cris_publications/7c7c920d-e5e5-4c53-9dfd-5738950629ca","doi":null,"is_oa":false,"landing_page_url":"https://research.manchester.ac.uk/en/publications/7c7c920d-e5e5-4c53-9dfd-5738950629ca","pdf_url":null,"source":{"id":"https://openalex.org/S4306400662","display_name":"Research Explorer (The University of Manchester)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I28407311","host_organization_name":"University of Manchester","host_organization_lineage":["https://openalex.org/I28407311"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Potier, D N 2012, 'The Development of Mass Spectrometry Based Approaches to Monitor Protease Activity in Biological Fluids', University of Manchester.","raw_type":"info:eu-repo/semantics/doctoralThesis"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5090337125","display_name":"David N. Potier","orcid":"https://orcid.org/0000-0001-5335-234X","observed_orcids":["https://orcid.org/0000-0001-5335-234X"]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"David N. Potier","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5090337125"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10683","display_name":"Mass Spectrometry Techniques and Applications","score":0.5249000191688538,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T10683","display_name":"Mass Spectrometry Techniques and Applications","score":0.5249000191688538,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10620","display_name":"Protease and Inhibitor Mechanisms","score":0.2628999948501587,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10519","display_name":"Advanced Proteomics Techniques and Applications","score":0.1809999942779541,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/asparaginyl-endopeptidase","display_name":"asparaginyl endopeptidase","score":0.9829999804496765},{"id":"https://openalex.org/keywords/caspase-3","display_name":"caspase-3","score":0.9860000014305115},{"id":"https://openalex.org/keywords/plasma-biomarkers","display_name":"plasma biomarkers","score":0.6819999814033508},{"id":"https://openalex.org/keywords/pediatric-leukemia","display_name":"pediatric leukemia","score":0.8579999804496765},{"id":"https://openalex.org/keywords/chemotherapy-response-prediction","display_name":"chemotherapy response prediction","score":0.5929999947547913},{"id":"https://openalex.org/keywords/peptide-enrichment","display_name":"peptide enrichment","score":0.8140000104904175},{"id":"https://openalex.org/keywords/tumor-response-prediction","display_name":"tumor response prediction","score":0.4620000123977661},{"id":"https://openalex.org/keywords/sample-preparation","display_name":"sample preparation","score":0.42500001192092896}],"concepts":[{"id":"https://openalex.org/C2776714187","wikidata":"https://www.wikidata.org/wiki/Q212410","display_name":"Protease","level":3,"score":0.7022610902786255},{"id":"https://openalex.org/C182220744","wikidata":"https://www.wikidata.org/wiki/Q7251226","display_name":"Proteases","level":3,"score":0.6197014451026917},{"id":"https://openalex.org/C2780394083","wikidata":"https://www.wikidata.org/wiki/Q539568","display_name":"Angiogenesis","level":2,"score":0.582979142665863},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.4991748332977295},{"id":"https://openalex.org/C3020728648","wikidata":"https://www.wikidata.org/wiki/Q1058795","display_name":"Biological fluids","level":2,"score":0.46987372636795044},{"id":"https://openalex.org/C60644358","wikidata":"https://www.wikidata.org/wiki/Q128570","display_name":"Bioinformatics","level":1,"score":0.4209284782409668},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.41397055983543396},{"id":"https://openalex.org/C143998085","wikidata":"https://www.wikidata.org/wiki/Q162555","display_name":"Oncology","level":1,"score":0.38938915729522705},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.33153069019317627},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.2676221430301666},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.2205868363380432},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.1722005009651184},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.14010697603225708},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.09890618920326233}],"mesh":[],"locations_count":3,"locations":[{"id":"pmh:oai:pure.atira.dk:openaire_cris_publications/7c7c920d-e5e5-4c53-9dfd-5738950629ca","doi":null,"is_oa":false,"landing_page_url":"https://research.manchester.ac.uk/en/publications/7c7c920d-e5e5-4c53-9dfd-5738950629ca","pdf_url":null,"source":{"id":"https://openalex.org/S4306400662","display_name":"Research Explorer (The University of Manchester)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I28407311","host_organization_name":"University of Manchester","host_organization_lineage":["https://openalex.org/I28407311"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Potier, D N 2012, 'The Development of Mass Spectrometry Based Approaches to Monitor Protease Activity in Biological Fluids', University of Manchester.","raw_type":"info:eu-repo/semantics/doctoralThesis"},{"id":"pmh:oai:pure.atira.dk:studenttheses/c864174e-370b-4e40-b19e-d63ee942d21b","doi":null,"is_oa":false,"landing_page_url":"https://research.manchester.ac.uk/en/studentTheses/c864174e-370b-4e40-b19e-d63ee942d21b","pdf_url":null,"source":{"id":"https://openalex.org/S4306400662","display_name":"Research Explorer (The University of Manchester)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I28407311","host_organization_name":"University of Manchester","host_organization_lineage":["https://openalex.org/I28407311"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"synchronisedUnifiedStudentThesis","raw_type":"Phd"},{"id":"mag:847718385","doi":null,"is_oa":false,"landing_page_url":"https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.606895","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.8371999859809875}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":244,"referenced_works":["https://openalex.org/W15817783","https://openalex.org/W52745064","https://openalex.org/W69356324","https://openalex.org/W101319026","https://openalex.org/W118909989","https://openalex.org/W1516914181","https://openalex.org/W1521130890","https://openalex.org/W1536690700","https://openalex.org/W1565050438","https://openalex.org/W1585782290","https://openalex.org/W1604468403","https://openalex.org/W1606241667","https://openalex.org/W1741429005","https://openalex.org/W1869042642","https://openalex.org/W1939128981","https://openalex.org/W1942214720","https://openalex.org/W1944430150","https://openalex.org/W1963528695","https://openalex.org/W1963652029","https://openalex.org/W1964083798","https://openalex.org/W1964753682","https://openalex.org/W1965667088","https://openalex.org/W1965807926","https://openalex.org/W1966955341","https://openalex.org/W1967955344","https://openalex.org/W1969222622","https://openalex.org/W1970458434","https://openalex.org/W1970724938","https://openalex.org/W1971614113","https://openalex.org/W1973190361","https://openalex.org/W1974830441","https://openalex.org/W1975304761","https://openalex.org/W1977211859","https://openalex.org/W1977219317","https://openalex.org/W1977291445","https://openalex.org/W1977293684","https://openalex.org/W1977799997","https://openalex.org/W1978890656","https://openalex.org/W1978902428","https://openalex.org/W1980135007","https://openalex.org/W1980593265","https://openalex.org/W1981740820","https://openalex.org/W1981989535","https://openalex.org/W1983169511","https://openalex.org/W1983391688","https://openalex.org/W1985619086","https://openalex.org/W1985694351","https://openalex.org/W1986656413","https://openalex.org/W1988695302","https://openalex.org/W1989627660","https://openalex.org/W1991865867","https://openalex.org/W1993912669","https://openalex.org/W1994159961","https://openalex.org/W1998192315","https://openalex.org/W1998404018","https://openalex.org/W1998754764","https://openalex.org/W1999609687","https://openalex.org/W2000025384","https://openalex.org/W2000798961","https://openalex.org/W2002503549","https://openalex.org/W2002664199","https://openalex.org/W2002844160","https://openalex.org/W2003191065","https://openalex.org/W2003603784","https://openalex.org/W2004114046","https://openalex.org/W2004588605","https://openalex.org/W2004709114","https://openalex.org/W2005832407","https://openalex.org/W2007070415","https://openalex.org/W2008181517","https://openalex.org/W2008219426","https://openalex.org/W2008789019","https://openalex.org/W2009321462","https://openalex.org/W2009886857","https://openalex.org/W2010385496","https://openalex.org/W2010996292","https://openalex.org/W2011226894","https://openalex.org/W2011603383","https://openalex.org/W2016222163","https://openalex.org/W2017265604","https://openalex.org/W2017415179","https://openalex.org/W2017469353","https://openalex.org/W2018104191","https://openalex.org/W2019827191","https://openalex.org/W2020691645","https://openalex.org/W2023325374","https://openalex.org/W2025085133","https://openalex.org/W2025422229","https://openalex.org/W2026988411","https://openalex.org/W2028615859","https://openalex.org/W2028995205","https://openalex.org/W2029249419","https://openalex.org/W2029539153","https://openalex.org/W2029715515","https://openalex.org/W2031132185","https://openalex.org/W2033290380","https://openalex.org/W2033492963","https://openalex.org/W2034533024","https://openalex.org/W2034887729","https://openalex.org/W2035309334","https://openalex.org/W2038266165","https://openalex.org/W2038554914","https://openalex.org/W2039215669","https://openalex.org/W2039578667","https://openalex.org/W2039994436","https://openalex.org/W2040481562","https://openalex.org/W2040980668","https://openalex.org/W2041173561","https://openalex.org/W2043064319","https://openalex.org/W2043564026","https://openalex.org/W2043980298","https://openalex.org/W2044363194","https://openalex.org/W2046700945","https://openalex.org/W2046713027","https://openalex.org/W2048428832","https://openalex.org/W2049874012","https://openalex.org/W2050358188","https://openalex.org/W2050834003","https://openalex.org/W2050840712","https://openalex.org/W2051658957","https://openalex.org/W2051773486","https://openalex.org/W2052177013","https://openalex.org/W2052222318","https://openalex.org/W2055724051","https://openalex.org/W2056640157","https://openalex.org/W2056974529","https://openalex.org/W2057541626","https://openalex.org/W2062203307","https://openalex.org/W2062222985","https://openalex.org/W2062268083","https://openalex.org/W2062499878","https://openalex.org/W2063292959","https://openalex.org/W2064543105","https://openalex.org/W2066969492","https://openalex.org/W2068042361","https://openalex.org/W2068481474","https://openalex.org/W2068902734","https://openalex.org/W2069720157","https://openalex.org/W2070686135","https://openalex.org/W2071054574","https://openalex.org/W2071095150","https://openalex.org/W2071397130","https://openalex.org/W2071772754","https://openalex.org/W2073292063","https://openalex.org/W2073619906","https://openalex.org/W2075517223","https://openalex.org/W2076234920","https://openalex.org/W2076534093","https://openalex.org/W2078594804","https://openalex.org/W2078798112","https://openalex.org/W2078846752","https://openalex.org/W2080719056","https://openalex.org/W2081242185","https://openalex.org/W2082656241","https://openalex.org/W2085006338","https://openalex.org/W2086149372","https://openalex.org/W2086652272","https://openalex.org/W2087367624","https://openalex.org/W2088023734","https://openalex.org/W2088063041","https://openalex.org/W2092007646","https://openalex.org/W2092245588","https://openalex.org/W2093448775","https://openalex.org/W2093916152","https://openalex.org/W2100531189","https://openalex.org/W2100894466","https://openalex.org/W2101608265","https://openalex.org/W2102408728","https://openalex.org/W2103113681","https://openalex.org/W2104077314","https://openalex.org/W2105099929","https://openalex.org/W2105192683","https://openalex.org/W2108516742","https://openalex.org/W2108804687","https://openalex.org/W2110837364","https://openalex.org/W2110921177","https://openalex.org/W2110925586","https://openalex.org/W2110992045","https://openalex.org/W2111336932","https://openalex.org/W2112066976","https://openalex.org/W2113032639","https://openalex.org/W2113432838","https://openalex.org/W2113725477","https://openalex.org/W2113982003","https://openalex.org/W2115700064","https://openalex.org/W2116537907","https://openalex.org/W2116986285","https://openalex.org/W2117044102","https://openalex.org/W2117298495","https://openalex.org/W2117570501","https://openalex.org/W2117692326","https://openalex.org/W2117977995","https://openalex.org/W2118437191","https://openalex.org/W2118656195","https://openalex.org/W2120161408","https://openalex.org/W2120384906","https://openalex.org/W2121421904","https://openalex.org/W2123999563","https://openalex.org/W2125045516","https://openalex.org/W2129493754","https://openalex.org/W2131755635","https://openalex.org/W2132813996","https://openalex.org/W2132915027","https://openalex.org/W2133000461","https://openalex.org/W2133669258","https://openalex.org/W2133858793","https://openalex.org/W2134575341","https://openalex.org/W2135196225","https://openalex.org/W2140132747","https://openalex.org/W2141580108","https://openalex.org/W2145828336","https://openalex.org/W2146647631","https://openalex.org/W2146743941","https://openalex.org/W2147098153","https://openalex.org/W2147107452","https://openalex.org/W2147886123","https://openalex.org/W2150978299","https://openalex.org/W2151501496","https://openalex.org/W2152576550","https://openalex.org/W2154139219","https://openalex.org/W2154174652","https://openalex.org/W2154571107","https://openalex.org/W2155470210","https://openalex.org/W2156808232","https://openalex.org/W2157455826","https://openalex.org/W2158639476","https://openalex.org/W2158883006","https://openalex.org/W2160209118","https://openalex.org/W2162450324","https://openalex.org/W2163221081","https://openalex.org/W2165454153","https://openalex.org/W2167395048","https://openalex.org/W2167531056","https://openalex.org/W2167718545","https://openalex.org/W2168209462","https://openalex.org/W2168386258","https://openalex.org/W2168717708","https://openalex.org/W2168939326","https://openalex.org/W2224671078","https://openalex.org/W2257165741","https://openalex.org/W2321659793","https://openalex.org/W2335385687","https://openalex.org/W2414477074","https://openalex.org/W2887142534"],"related_works":["https://openalex.org/W2807529093","https://openalex.org/W2083392654","https://openalex.org/W3213787075","https://openalex.org/W6931717569","https://openalex.org/W2607429552","https://openalex.org/W7222963810","https://openalex.org/W2158552530","https://openalex.org/W7112539449","https://openalex.org/W184197221","https://openalex.org/W6903462381"],"abstract_inverted_index":{"When":[0],"treating":[1],"patients":[2],"with":[3,180],"cancer,":[4],"the":[5,28,115,128,133,170,178,189,258,269,279,316,334,344,349,371],"ability":[6],"to":[7,12,18,21,34,55,85,94,114,120,127,139,153,156,184,187,202,207,248,260,277,300,340,360,375],"predict":[8,203],"a":[9,32,36,40,52,56,91,173,199,204,241,272,324,383],"patient\u2019s":[10,37,53,92,174,205],"response":[11,38,54,93,155,206,303],"treatment":[13],"is":[14,43,75,235,240,419],"an":[15,48,87,166,230,389],"important":[16],"tool":[17],"allow":[19],"therapy":[20],"be":[22,261,341],"tailored":[23],"for":[24,31,363],"best":[25,186,359,372],"outcome.":[26],"Therefore,":[27,165,191,229],"need":[29],"exists":[30],"test":[33],"forecast":[35],"using":[39,292],"sample":[41,327],"that":[42],"readily":[44,80],"accessible,":[45],"and":[46,82,130,141,226,290,307,318,329,388,403],"provides":[47],"accurate":[49,88],"reflection":[50],"of":[51,90,117,132,172,271,281,320,323,336,351,409],"disease":[57],"or":[58,65,106],"treatment.Profiling":[59],"biological":[60,252,398],"fluids,":[61],"such":[62,400],"as":[63,158,160,183,198,367,369,401],"plasma":[64,402,417],"urine,":[66],"has":[67,100,111,150,195,257],"gained":[68],"considerable":[69],"interest":[70],"in":[71,123,251,263,284,304,397,412,416],"recent":[72],"years.":[73],"This":[74,312,332],"because":[76],"these":[77,220,364,377],"fluids":[78],"are":[79,83,213],"available":[81],"expected":[84],"provide":[86,177],"representation":[89],"treatment.":[95],"As":[96,254],"such,":[97,255],"much":[98],"effort":[99],"been":[101,112,151,196,298],"put":[102],"into":[103],"finding":[104],"biomarkers":[105],"prognostic":[107],"indicators.Abnormal":[108],"protease":[109,148,193],"activity":[110,149,171,194,211,265,280,396,415],"linked":[113,152,299],"progression":[116],"cancer":[118,208],"due":[119],"their":[121],"involvement":[122],"several":[124],"processes":[125],"vital":[126],"survival":[129],"proliferation":[131],"disease.":[134],"These":[135,295],"include":[136],"metastasis,":[137],"resistance":[138],"apoptosis":[140],"angiogenesis,":[142],"amongst":[143],"others.":[144],"In":[145],"addition,":[146,379],"dysregulated":[147],"poor":[154,301],"chemotherapy":[157],"well":[159,368],"tumour":[161,308],"regrowth":[162,309],"following":[163],"radiotherapy.":[164],"increased":[167],"understanding":[168],"regarding":[169],"proteases":[175],"may":[176],"clinician":[179],"more":[181,232],"information":[182],"how":[185,358],"treat":[188],"patient.":[190],"monitoring":[192,394],"suggested":[197],"potential":[200,259],"marker":[201],"treatment.Most":[209],"enzyme":[210,264,337],"assays":[212],"currently":[214],"performed":[215],"by":[216,343],"fluorescence":[217,391],"spectroscopy.":[218],"However,":[219],"workflows":[221],"suffer":[222],"from":[223],"limited":[224],"sensitivity":[225],"linear":[227],"range.":[228],"alternative,":[231],"sensitive":[233,243],"assay":[234,387,392],"required.":[236],"Mass":[237],"spectrometry":[238],"(MS)":[239],"highly":[242],"analytical":[244],"technique":[245,374],"routinely":[246],"used":[247,262],"quantify":[249],"changes":[250],"systems.":[253],"MS":[256,274,373],"assays.This":[266],"study":[267],"illustrates":[268],"development":[270,317],"novel":[273],"based":[275],"method":[276,411],"monitor":[278,376],"target":[282,345,365],"enzymes":[283,296,353],"plasma;":[285],"specifically":[286],"asparaginyl":[287],"endopeptidase":[288],"(AEP)":[289],"caspase-3":[291,414],"mass":[293],"spectrometry.":[294],"have":[297],"chemotherapeutic":[302],"childhood":[305],"leukaemia":[306],"post-radiotherapy":[310],"respectively.":[311],"project":[313,381],"will":[314],"describe":[315],"optimisation":[319],"each":[321],"stage":[322],"five":[325],"step":[326],"preparation":[328],"analysis":[330],"method.":[331],"includes":[333],"design":[335],"substrates":[338],"designed":[339],"cleaved":[342],"enzyme,":[346],"whilst":[347],"reducing":[348],"effect":[350],"other":[352],"acting":[354],"on":[355],"this":[356,380,386,410],"substrate,":[357],"enrich":[361],"samples":[362,399,418],"peptides,":[366],"determining":[370],"peptides.In":[378],"describes":[382],"comparison":[384],"between":[385],"existing":[390],"when":[393],"AEP":[395],"whole":[404],"cell":[405],"lysates.":[406],"The":[407],"application":[408],"quantifying":[413],"also":[420],"examined.":[421]},"counts_by_year":[],"updated_date":"2026-10-12T09:40:04.860316","created_date":"2016-06-24T00:00:00"}