{"id":"https://openalex.org/W84396783","doi":"https://doi.org/10.1016/s0021-9258(17)38790-2","title":"Characterization of a dermatan sulfate proteoglycan synthesized by murine parietal yolk sac (PYS-2) cells.","display_name":"Characterization of a dermatan sulfate proteoglycan synthesized by murine parietal yolk sac (PYS-2) cells.","publication_year":1985,"publication_date":"1985-11-01","ids":{"openalex":"https://openalex.org/W84396783","doi":"https://doi.org/10.1016/s0021-9258(17)38790-2","mag":"84396783","pmid":"https://pubmed.ncbi.nlm.nih.gov/4055755"},"language":"en","primary_location":{"id":"doi:10.1016/s0021-9258(17)38790-2","doi":"https://doi.org/10.1016/s0021-9258(17)38790-2","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0021-9258(17)38790-2","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"https://doi.org/10.1016/s0021-9258(17)38790-2","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5051382096","display_name":"John Couchman","orcid":"https://orcid.org/0000-0003-3409-7093","observed_orcids":["https://orcid.org/0000-0003-3409-7093"]},"institutions":[{"id":"https://openalex.org/I124055696","display_name":"University of Copenhagen","ror":"https://ror.org/035b05819","country_code":"DK","type":"education","lineage":["https://openalex.org/I124055696"]}],"countries":["DK"],"is_corresponding":false,"raw_author_name":"J R Couchman","raw_affiliation_strings":["Couchman Group, BRIC, Faculty of Health and Medical Sciences, K\u00f8benhavns Universitet"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Couchman Group, BRIC, Faculty of Health and Medical Sciences, K\u00f8benhavns Universitet","institution_ids":["https://openalex.org/I124055696"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5046605351","display_name":"A Woods","orcid":"https://orcid.org/0009-0009-3538-0393","observed_orcids":["https://orcid.org/0009-0009-3538-0393"]},"institutions":[{"id":"https://openalex.org/I32389192","display_name":"University of Alabama at Birmingham","ror":"https://ror.org/008s83205","country_code":"US","type":"education","lineage":["https://openalex.org/I32389192"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"A Woods","raw_affiliation_strings":["University of Alabama at, Birmingham"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University of Alabama at, Birmingham","institution_ids":["https://openalex.org/I32389192"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5110274784","display_name":"Magnus H\u00f6\u00f6k","orcid":"https://orcid.org/0000-0003-2703-0309","observed_orcids":["https://orcid.org/0000-0003-2703-0309"]},"institutions":[{"id":"https://openalex.org/I1295876152","display_name":"Houston Methodist","ror":"https://ror.org/027zt9171","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1295876152"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"M H\u00f6\u00f6k","raw_affiliation_strings":["Houston Methodist"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Houston Methodist","institution_ids":["https://openalex.org/I1295876152"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5091534077","display_name":"James E. Christner","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I32389192","display_name":"University of Alabama at Birmingham","ror":"https://ror.org/008s83205","country_code":"US","type":"education","lineage":["https://openalex.org/I32389192"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"J E Christner","raw_affiliation_strings":["University of Alabama at, Birmingham"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University of Alabama at, Birmingham","institution_ids":["https://openalex.org/I32389192"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":3,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":3430,"currency":"USD","value_usd":3430},"apc_paid":{"value":3430,"currency":"USD","value_usd":3430},"fwci":1.9062,"has_fulltext":false,"cited_by_count":35,"citation_normalized_percentile":{"value":0.8473122,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":94},"biblio":{"volume":"260","issue":"25","first_page":"13755","last_page":"13762"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11131","display_name":"Proteoglycans and glycosaminoglycans research","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11131","display_name":"Proteoglycans and glycosaminoglycans research","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10869","display_name":"Polysaccharides and Plant Cell Walls","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10105","display_name":"Osteoarthritis Treatment and Mechanisms","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2745","display_name":"Rheumatology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/dermatan-sulfate-proteoglycan","display_name":"dermatan sulfate proteoglycan","score":0.9900000095367432},{"id":"https://openalex.org/keywords/basement-membrane","display_name":"basement membrane","score":0.5320000052452087},{"id":"https://openalex.org/keywords/iduronic-acid","display_name":"iduronic acid","score":0.871999979019165},{"id":"https://openalex.org/keywords/glucuronic-acid","display_name":"glucuronic acid","score":0.8059999942779541},{"id":"https://openalex.org/keywords/core-protein","display_name":"core protein","score":0.6520000100135803},{"id":"https://openalex.org/keywords/proteoglycan-synthesis","display_name":"proteoglycan synthesis","score":0.8019999861717224},{"id":"https://openalex.org/keywords/chondroitin-sulfate-proteoglycans","display_name":"chondroitin sulfate proteoglycans","score":0.8830000162124634},{"id":"https://openalex.org/keywords/cartilage-proteoglycans","display_name":"cartilage proteoglycans","score":0.7319999933242798}],"concepts":[{"id":"https://openalex.org/C2778066728","wikidata":"https://www.wikidata.org/wiki/Q2335618","display_name":"Dermatan sulfate","level":4,"score":0.8372163772583008},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.6238236427307129},{"id":"https://openalex.org/C2779335624","wikidata":"https://www.wikidata.org/wiki/Q407976","display_name":"Proteoglycan","level":3,"score":0.4919867217540741},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.4880727529525757},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.4513673782348633},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.4255490303039551},{"id":"https://openalex.org/C153074725","wikidata":"https://www.wikidata.org/wiki/Q407553","display_name":"Glycosaminoglycan","level":2,"score":0.3354042172431946},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.26063475012779236},{"id":"https://openalex.org/C2778041096","wikidata":"https://www.wikidata.org/wiki/Q424668","display_name":"Heparan sulfate","level":3,"score":0.20635265111923218},{"id":"https://openalex.org/C189165786","wikidata":"https://www.wikidata.org/wiki/Q193825","display_name":"Extracellular matrix","level":2,"score":0.11215025186538696}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000911","descriptor_name":"Antibodies, Monoclonal","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D001485","descriptor_name":"Basement Membrane","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D002460","descriptor_name":"Cell Line","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002807","descriptor_name":"Chondroitin","qualifier_ui":"Q000031","qualifier_name":"analogs & derivatives","is_major_topic":true},{"descriptor_ui":"D002850","descriptor_name":"Chromatography, Gel","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002852","descriptor_name":"Chromatography, Ion Exchange","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003871","descriptor_name":"Dermatan Sulfate","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D003871","descriptor_name":"Dermatan Sulfate","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D003871","descriptor_name":"Dermatan Sulfate","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D008970","descriptor_name":"Molecular Weight","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011506","descriptor_name":"Proteins","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D015017","descriptor_name":"Yolk Sac","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1016/s0021-9258(17)38790-2","doi":"https://doi.org/10.1016/s0021-9258(17)38790-2","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0021-9258(17)38790-2","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},{"id":"pmid:4055755","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/4055755","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of biological chemistry","raw_type":"Journal Article"},{"id":"pmh:oai:pure.atira.dk:openaire_cris_publications/8c0f0560-5985-11dd-8d9f-000ea68e967b","doi":null,"is_oa":false,"landing_page_url":"https://researchprofiles.ku.dk/da/publications/8c0f0560-5985-11dd-8d9f-000ea68e967b","pdf_url":null,"source":{"id":"https://openalex.org/S4306401983","display_name":"Research at the University of Copenhagen (University of Copenhagen)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I124055696","host_organization_name":"University of Copenhagen","host_organization_lineage":["https://openalex.org/I124055696"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Couchman, J R, Woods, A, H\u00f6\u00f6k, M & Christner, J E 1985, 'Characterization of a dermatan sulfate proteoglycan synthesized by murine parietal yolk sac (PYS-2) cells.', Journal of Biological Chemistry, vol. 260, no. 25, pp. 13755-62.","raw_type":"info:eu-repo/semantics/article"}],"best_oa_location":{"id":"doi:10.1016/s0021-9258(17)38790-2","doi":"https://doi.org/10.1016/s0021-9258(17)38790-2","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0021-9258(17)38790-2","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G1091045299","display_name":"STRUCTURE AND FUNCTIONS OF THE EXTRACELLULAR MATRIX","funder_award_id":"5r01am027807-06","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"},{"id":"https://openalex.org/G4963941182","display_name":"THE CNBR CLEAVAGE OF CHONDROSARCOMA PROTEOGLYCAN","funder_award_id":"5r01am027308-06","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"}],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":29,"referenced_works":["https://openalex.org/W1484375997","https://openalex.org/W1492080952","https://openalex.org/W1493384057","https://openalex.org/W1499644887","https://openalex.org/W1551601138","https://openalex.org/W1600027580","https://openalex.org/W1832131361","https://openalex.org/W1980617596","https://openalex.org/W1980741458","https://openalex.org/W1998741154","https://openalex.org/W2008553112","https://openalex.org/W2013100111","https://openalex.org/W2014856172","https://openalex.org/W2034353120","https://openalex.org/W2041420763","https://openalex.org/W2056886140","https://openalex.org/W2061458349","https://openalex.org/W2063571042","https://openalex.org/W2069389359","https://openalex.org/W2077400092","https://openalex.org/W2097278018","https://openalex.org/W2116786132","https://openalex.org/W2146923243","https://openalex.org/W2164188238","https://openalex.org/W2233128941","https://openalex.org/W2236436466","https://openalex.org/W2260545696","https://openalex.org/W2306845595","https://openalex.org/W2409735216"],"related_works":["https://openalex.org/W2040995136","https://openalex.org/W1525955379","https://openalex.org/W1517128119","https://openalex.org/W2009175116","https://openalex.org/W1593791665","https://openalex.org/W1990345193","https://openalex.org/W2067796933","https://openalex.org/W2014941259","https://openalex.org/W1573262794","https://openalex.org/W1600027580"],"abstract_inverted_index":{"A":[0],"dermatan":[1,32,157],"sulfate":[2,33,43,58,158,164],"proteoglycan":[3,23,93,110,132,140,165],"has":[4,24],"been":[5],"isolated":[6],"from":[7,105,136],"a":[8,25,121],"murine":[9],"parietal":[10],"yolk":[11],"sac":[12],"cell":[13],"line,":[14],"which":[15],"in":[16,141,168],"culture":[17],"synthesizes":[18],"basement":[19,171],"membrane":[20],"components.":[21],"The":[22,39],"molecular":[26,98],"weight":[27],"of":[28,35,49,56,67,73,89,100,108,114,124,145],"200,000-300,000":[29],"with":[30,97],"10-15":[31],"chains":[34,41,117],"Mr":[36],"=":[37,129],"14,000-16,000.":[38],"glycosaminoglycan":[40],"carry":[42],"residues":[44,71],"predominantly":[45],"attached":[46],"to":[47,152,161],"C-4":[48],"the":[50,57,68,74,77,109,115,125,137,142,162],"galactosamine":[51,63],"unit;":[52],"less":[53],"than":[54],"10%":[55],"groups":[59],"occur":[60],"as":[61],"6-sulfated":[62],"units.":[64],"About":[65],"60%":[66],"uronic":[69],"acid":[70],"are":[72,118],"glucuronic":[75],"configuration,":[76],"rest":[78],"being":[79],"iduronic":[80],"acid.":[81],"Analysis":[82],"by":[83],"sodium":[84],"dodecyl":[85],"sulfate-polyacrylamide":[86],"gel":[87],"electrophoresis":[88],"chondroitinase":[90],"ABC-treated":[91],"125I-labeled":[92],"reveals":[94],"two":[95],"polypeptides":[96],"weights":[99],"34,000":[101],"and":[102,149,156,160],"27,000.":[103],"Results":[104],"papain":[106],"digestion":[107],"suggest":[111],"that":[112],"most":[113],"polysaccharide":[116],"clustered":[119],"at":[120],"papain-resistant":[122],"segment":[123],"core":[126,147],"protein":[127],"(Mr":[128],"8,000).":[130],"This":[131],"is":[133],"distinctly":[134],"different":[135],"large":[138],"cartilage":[139],"smaller":[143],"size":[144],"its":[146,150],"protein,":[148],"relationship":[151],"other":[153],"small":[154],"chondroitin":[155,163],"proteoglycans":[159],"recently":[166],"located":[167],"rat":[169],"tissue":[170],"membranes":[172],"will":[173],"be":[174],"discussed.":[175]},"counts_by_year":[{"year":2015,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}