{"id":"https://openalex.org/W841130146","doi":null,"title":"Biophysical and Biochemical Studies of the FMDV and EMCV IRES Elements","display_name":"Biophysical and Biochemical Studies of the FMDV and EMCV IRES Elements","publication_year":2013,"publication_date":"2013-12-06","ids":{"openalex":"https://openalex.org/W841130146","mag":"841130146"},"language":"en","primary_location":{"id":"pmh:oai:pure.atira.dk:studenttheses/60a0fb5a-157b-4a26-83f4-1d2eb3e32002","doi":null,"is_oa":false,"landing_page_url":"https://research.manchester.ac.uk/en/studentTheses/60a0fb5a-157b-4a26-83f4-1d2eb3e32002","pdf_url":null,"source":{"id":"https://openalex.org/S4306400662","display_name":"Research Explorer (The University of Manchester)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I28407311","host_organization_name":"University of Manchester","host_organization_lineage":["https://openalex.org/I28407311"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"synchronisedUnifiedStudentThesis","raw_type":"Phd"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5016519374","display_name":"Anthonia Chinwe Onuegbu","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Anthonia Chinwe Onuegbu","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5016519374"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":0.9911999702453613,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":0.9911999702453613,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10432","display_name":"DNA and Nucleic Acid Chemistry","score":0.0019000000320374966,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.0012000000569969416,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/foot-and-mouth-disease-virus","display_name":"foot-and-mouth disease virus","score":0.7089999914169312},{"id":"https://openalex.org/keywords/encephalomyocarditis-virus","display_name":"encephalomyocarditis virus","score":0.9120000004768372},{"id":"https://openalex.org/keywords/internal-ribosome-entry-site","display_name":"internal ribosome entry site","score":0.902999997138977},{"id":"https://openalex.org/keywords/rna-hairpin-loops","display_name":"RNA hairpin loops","score":0.5950000286102295},{"id":"https://openalex.org/keywords/rna-folding-thermodynamics","display_name":"RNA folding thermodynamics","score":0.640999972820282},{"id":"https://openalex.org/keywords/melting-point","display_name":"melting point","score":0.6769999861717224},{"id":"https://openalex.org/keywords/magnesium-binding","display_name":"magnesium binding","score":0.7179999947547913},{"id":"https://openalex.org/keywords/rna-secondary-structure","display_name":"RNA secondary structure","score":0.46000000834465027},{"id":"https://openalex.org/keywords/circular-dichroism","display_name":"circular dichroism","score":0.6290000081062317},{"id":"https://openalex.org/keywords/uv-melting","display_name":"UV melting","score":0.8939999938011169},{"id":"https://openalex.org/keywords/viral-translation-initiation","display_name":"viral translation initiation","score":0.4000000059604645},{"id":"https://openalex.org/keywords/rna-stability","display_name":"RNA stability","score":0.38600000739097595}],"concepts":[{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.7972077131271362},{"id":"https://openalex.org/C2985276288","wikidata":"https://www.wikidata.org/wiki/Q16873139","display_name":"Melting temperature","level":2,"score":0.6470044851303101},{"id":"https://openalex.org/C133571119","wikidata":"https://www.wikidata.org/wiki/Q899102","display_name":"Circular dichroism","level":2,"score":0.6125262975692749},{"id":"https://openalex.org/C77430603","wikidata":"https://www.wikidata.org/wiki/Q166992","display_name":"Internal ribosome entry site","level":5,"score":0.5507869124412537},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4957057237625122},{"id":"https://openalex.org/C8010536","wikidata":"https://www.wikidata.org/wiki/Q160398","display_name":"Crystallography","level":1,"score":0.4273584485054016},{"id":"https://openalex.org/C190232843","wikidata":"https://www.wikidata.org/wiki/Q1950808","display_name":"Small hairpin RNA","level":4,"score":0.4219292998313904},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.3702983260154724},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.3252027928829193},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.32188868522644043},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.21131014823913574},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.18806025385856628},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.1790654957294464},{"id":"https://openalex.org/C88478588","wikidata":"https://www.wikidata.org/wiki/Q42244","display_name":"Ribosome","level":4,"score":0.0971546471118927},{"id":"https://openalex.org/C159985019","wikidata":"https://www.wikidata.org/wiki/Q181790","display_name":"Composite material","level":1,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:pure.atira.dk:studenttheses/60a0fb5a-157b-4a26-83f4-1d2eb3e32002","doi":null,"is_oa":false,"landing_page_url":"https://research.manchester.ac.uk/en/studentTheses/60a0fb5a-157b-4a26-83f4-1d2eb3e32002","pdf_url":null,"source":{"id":"https://openalex.org/S4306400662","display_name":"Research Explorer (The University of Manchester)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I28407311","host_organization_name":"University of Manchester","host_organization_lineage":["https://openalex.org/I28407311"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"synchronisedUnifiedStudentThesis","raw_type":"Phd"},{"id":"mag:841130146","doi":null,"is_oa":false,"landing_page_url":"https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.607081","pdf_url":null,"source":{"id":"https://openalex.org/S4306532718","display_name":"[Thesis]. Manchester, UK: The University of                    Manchester; 2013.","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"[Thesis]. Manchester, UK: The University of Manchester; 2013.","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":98,"referenced_works":["https://openalex.org/W69657478","https://openalex.org/W381708633","https://openalex.org/W654592591","https://openalex.org/W1484115349","https://openalex.org/W1502421630","https://openalex.org/W1600318172","https://openalex.org/W1606248456","https://openalex.org/W1694273832","https://openalex.org/W1807212217","https://openalex.org/W1870070035","https://openalex.org/W1963663478","https://openalex.org/W1967369040","https://openalex.org/W1968280735","https://openalex.org/W1972194009","https://openalex.org/W1973891814","https://openalex.org/W1974951196","https://openalex.org/W1982539830","https://openalex.org/W1983614124","https://openalex.org/W1984002397","https://openalex.org/W1985092367","https://openalex.org/W1985654383","https://openalex.org/W1988409793","https://openalex.org/W1989173346","https://openalex.org/W1991366673","https://openalex.org/W1992146768","https://openalex.org/W1998301160","https://openalex.org/W2006640104","https://openalex.org/W2012398587","https://openalex.org/W2017526934","https://openalex.org/W2018999693","https://openalex.org/W2019647067","https://openalex.org/W2025171497","https://openalex.org/W2028346256","https://openalex.org/W2032930812","https://openalex.org/W2033648778","https://openalex.org/W2042386529","https://openalex.org/W2048731469","https://openalex.org/W2048962220","https://openalex.org/W2050591121","https://openalex.org/W2056559695","https://openalex.org/W2056683465","https://openalex.org/W2057080108","https://openalex.org/W2058627247","https://openalex.org/W2061745754","https://openalex.org/W2063675437","https://openalex.org/W2065305397","https://openalex.org/W2065584208","https://openalex.org/W2066906496","https://openalex.org/W2067602907","https://openalex.org/W2068830076","https://openalex.org/W2075554307","https://openalex.org/W2078224461","https://openalex.org/W2081661452","https://openalex.org/W2083152645","https://openalex.org/W2083281404","https://openalex.org/W2085158278","https://openalex.org/W2085794569","https://openalex.org/W2093915148","https://openalex.org/W2097897069","https://openalex.org/W2098477883","https://openalex.org/W2101195721","https://openalex.org/W2102060245","https://openalex.org/W2102754190","https://openalex.org/W2103084023","https://openalex.org/W2106882534","https://openalex.org/W2109027308","https://openalex.org/W2109941922","https://openalex.org/W2111699871","https://openalex.org/W2112647321","https://openalex.org/W2113508801","https://openalex.org/W2116131557","https://openalex.org/W2117504839","https://openalex.org/W2118956677","https://openalex.org/W2119549758","https://openalex.org/W2122377457","https://openalex.org/W2123551500","https://openalex.org/W2126881800","https://openalex.org/W2128561929","https://openalex.org/W2136426050","https://openalex.org/W2136630070","https://openalex.org/W2137293540","https://openalex.org/W2137450588","https://openalex.org/W2137490166","https://openalex.org/W2141157874","https://openalex.org/W2148729457","https://openalex.org/W2150771961","https://openalex.org/W2152035630","https://openalex.org/W2155201844","https://openalex.org/W2157497892","https://openalex.org/W2159343050","https://openalex.org/W2161420671","https://openalex.org/W2161582274","https://openalex.org/W2163451322","https://openalex.org/W2163478476","https://openalex.org/W2165810116","https://openalex.org/W2171940869","https://openalex.org/W2214577495","https://openalex.org/W2491135945"],"related_works":["https://openalex.org/W758678171","https://openalex.org/W2161582274","https://openalex.org/W810677933","https://openalex.org/W619333751","https://openalex.org/W2105477691","https://openalex.org/W2261355828","https://openalex.org/W2081661452","https://openalex.org/W2097000233","https://openalex.org/W2109307901","https://openalex.org/W2123551500"],"abstract_inverted_index":{"The":[0,28,68,77,93,175,249,257,267,315,355],"biophysical":[1],"properties":[2],"of":[3,32,40,83,147,152,164,178,190,251,331,343,352,358,371,390,398,425,435,450],"the":[4,9,33,46,50,57,61,66,72,81,84,90,108,131,144,148,153,165,171,179,184,196,201,222,224,227,235,254,261,284,305,322,337,359,369,379,391,396,401,404,409,422,426,440,444,446],"hairpin":[5,41,52,405],"loop":[6,42,406],"structures":[7,43,407],"in":[8,49,60,65,71,368,408],"FMDV":[10,62,117,166,181,209,293,338],"and":[11,16,24,55,63,75,104,115,121,127,137,160,207,217,239,245,280,291,296,301,310,346,393,417,448],"EMCV":[12,139,312,325,347,360],"IRES":[13,167,410],"domains":[14],"three":[15],"I,":[17],"J":[18],"were":[19,87,271],"studied":[20],"using":[21,89],"UV":[22,415],"melting":[23,150,176,188,229,268,356,416],"Circular":[25],"Dichroism":[26],"studies.":[27,419],"apical":[29],"region":[30,35],"consists":[31],"hammerhead":[34],"that":[36],"is":[37,195,400],"made":[38],"up":[39],"such":[44],"as":[45],"GNRA":[47],"motifs":[48,86,163,263,295,327,381],"16mer":[51,323],"loop,":[53],"(FMDV":[54,74],"EMCV),":[56],"heptaloop":[58],"15mer":[59,339],"17mer":[64,225,348],"EMCV,":[67,223],"RAAA":[69],"motif":[70],"11mer":[73,116,161,292],"EMCV).":[76],"empirical":[78],"calculations":[79],"on":[80,260,421,433],"folding":[82],"RNA":[85,118,140,162,182,210,255,262,294,313,326,380],"determined":[88,169,364],"Mfold":[91],"program.":[92],"Gibb?s":[94],"energy":[95],"obtained":[96,270],"was":[97,168,183,264,319,362,373],"-62.5,":[98],"-41.9,":[99],"-19.3,":[100],"-7.1,":[101],"-3.3,":[102],"-5.9":[103],"-2.1":[105],"kcal/mol":[106,129],"for":[107,130,283,304],"141,":[109,154,202,285],"79,":[110,155,203,286],"36,":[111,156,205,287],"16,":[112,132,157,206,236,288,306],"15,":[113,158,289],"12":[114,159,290],"motifs,":[119,141],"respectively":[120],"-10.1,":[122],"-6.6,":[123],"-6.8,":[124],"-14.1,":[125],"-17.3,":[126],"-90.7":[128],"17,":[133,307],"28,":[134,308],"39,":[135],"44":[136,309],"88mer":[138],"respectively,":[142],"demonstrating":[143],"thermodynamic":[145,431],"stability":[146,424],"motifs.The":[149],"temperature":[151,177,189,230,357],"by":[170,200,234,273,336,414],"first":[172,402],"derivative":[173],"technique.":[174],"12mer":[180],"highest":[185,228],"with":[186,211,231,241,321,340,387,395],"a":[187,329,341,350],"78.0":[191,232],"\ufffdC":[192,282,303,334,345],"signifying":[193],"it":[194],"most":[197,316],"stable":[198],"followed":[199,233,335],"11,":[204],"15mers":[208],"76.0,":[212,213,243],"74.0,":[214],"72.0,":[215],"70.0":[216,218],"\ufffdC,":[219,247],"respectively.":[220,248],"In":[221],"had":[226],"88,":[237],"28":[238],"44mers":[240],"77.0,":[242],"76.0":[244,374],"74.0":[246],"addition":[250,397,442],"Mg2+":[252,372],"stabilised":[253],"motifs.":[256,314],"stabilisation":[258,389],"effect":[259,318],"not":[265,363],"uniform.":[266],"temperatures":[269],"increased":[272],"1.0,":[274,275],"4.0,":[276,298,299],"8.0,":[277,332],"5.0,":[278,297],"2.0":[279,281,300],"0":[302],"88mers":[311],"significant":[317],"observed":[320],"FMDV,":[324],"showing":[328,349],"difference":[330,342,351],"5.0":[333,344],"4.0":[353],"\ufffdC.":[354],"39mer":[361],"without":[365],"Mg2+,":[366],"but":[367],"presence":[370],"\ufffdC.The":[375],"CD":[376,418],"studies":[377],"showed":[378],"to":[382,443],"have":[383],"an":[384],"A-form":[385],"conformation":[386],"further":[388],"loops":[392],"stems":[394],"Mg2+.This":[399],"time":[403],"will":[411,429],"be":[412],"characterised":[413],"Information":[420],"thermal":[423],"central":[427],"domain":[428],"provide":[430],"information":[432],"mechanisms":[434],"inhibiting":[436],"protein":[437,452],"initiation":[438],"via":[439],"IRES.In":[441],"above,":[445],"expression":[447],"purification":[449],"eIF4GcII":[451],"has":[453],"been":[454],"carried":[455],"out.":[456]},"counts_by_year":[],"updated_date":"2025-10-10T17:16:08.811792","created_date":"2016-06-24T00:00:00"}