{"id":"https://openalex.org/W822000153","doi":null,"title":"Not Just a Passive Adaptor, the Periplasmic Component CusB of Escherichia coli's CusCFBA Copper Efflux System has an Active Functional Role","display_name":"Not Just a Passive Adaptor, the Periplasmic Component CusB of Escherichia coli's CusCFBA Copper Efflux System has an Active Functional Role","publication_year":2008,"publication_date":"2008-01-01","ids":{"openalex":"https://openalex.org/W822000153","mag":"822000153"},"language":"en","primary_location":{"id":"pmh:oai:repository.arizona.edu:10150/193554","doi":null,"is_oa":false,"landing_page_url":"http://hdl.handle.net/10150/193554","pdf_url":null,"source":{"id":"https://openalex.org/S4306400271","display_name":"UA Campus Repository (The University of Arizona)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I138006243","host_organization_name":"University of Arizona","host_organization_lineage":["https://openalex.org/I138006243"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":null,"raw_type":"Electronic Dissertation"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5024291266","display_name":"Ireena Bagai","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Ireena Bagai","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5024291266"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.6958000063896179,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.6958000063896179,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10147","display_name":"Antibiotic Resistance in Bacteria","score":0.14149999618530273,"subfield":{"id":"https://openalex.org/subfields/2726","display_name":"Microbiology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10570","display_name":"Drug Transport and Resistance Mechanisms","score":0.13609999418258667,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/escherichia-coli","display_name":"Escherichia coli","score":0.8539999723434448},{"id":"https://openalex.org/keywords/copper-efflux","display_name":"copper efflux","score":0.7789999842643738},{"id":"https://openalex.org/keywords/metallochaperone","display_name":"metallochaperone","score":0.9340000152587891},{"id":"https://openalex.org/keywords/metal-binding-sites","display_name":"metal binding sites","score":0.4740000069141388},{"id":"https://openalex.org/keywords/conformational-change","display_name":"conformational change","score":0.7829999923706055},{"id":"https://openalex.org/keywords/antibiotic-resistance","display_name":"antibiotic resistance","score":0.5960000157356262},{"id":"https://openalex.org/keywords/metal-resistance","display_name":"metal resistance","score":0.5329999923706055}],"concepts":[{"id":"https://openalex.org/C201663137","wikidata":"https://www.wikidata.org/wiki/Q21198990","display_name":"Periplasmic space","level":4,"score":0.8547292947769165},{"id":"https://openalex.org/C200082930","wikidata":"https://www.wikidata.org/wiki/Q22327148","display_name":"Efflux","level":2,"score":0.6595867872238159},{"id":"https://openalex.org/C544778455","wikidata":"https://www.wikidata.org/wiki/Q753","display_name":"Copper","level":2,"score":0.6157020330429077},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.6115208268165588},{"id":"https://openalex.org/C168167062","wikidata":"https://www.wikidata.org/wiki/Q1117970","display_name":"Component (thermodynamics)","level":2,"score":0.4897797107696533},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4435420632362366},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.4307931363582611},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3524113595485687},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.2690609097480774},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.11940175294876099},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.07337352633476257},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0},{"id":"https://openalex.org/C97355855","wikidata":"https://www.wikidata.org/wiki/Q11473","display_name":"Thermodynamics","level":1,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:repository.arizona.edu:10150/193554","doi":null,"is_oa":false,"landing_page_url":"http://hdl.handle.net/10150/193554","pdf_url":null,"source":{"id":"https://openalex.org/S4306400271","display_name":"UA Campus Repository (The University of Arizona)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I138006243","host_organization_name":"University of Arizona","host_organization_lineage":["https://openalex.org/I138006243"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":null,"raw_type":"Electronic Dissertation"},{"id":"mag:822000153","doi":null,"is_oa":false,"landing_page_url":"http://arizona.openrepository.com/arizona/handle/10150/193554","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.4000000059604645}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":180,"referenced_works":["https://openalex.org/W4926351","https://openalex.org/W10876090","https://openalex.org/W24256299","https://openalex.org/W107689395","https://openalex.org/W150547940","https://openalex.org/W163080191","https://openalex.org/W180079098","https://openalex.org/W183889290","https://openalex.org/W192874482","https://openalex.org/W204977290","https://openalex.org/W1448496890","https://openalex.org/W1496283406","https://openalex.org/W1500957784","https://openalex.org/W1528606854","https://openalex.org/W1529130961","https://openalex.org/W1555682241","https://openalex.org/W1558213377","https://openalex.org/W1585758755","https://openalex.org/W1586738204","https://openalex.org/W1608044990","https://openalex.org/W1629995527","https://openalex.org/W1653505145","https://openalex.org/W1664552158","https://openalex.org/W1726597626","https://openalex.org/W1879184430","https://openalex.org/W1885185292","https://openalex.org/W1892148740","https://openalex.org/W1896531654","https://openalex.org/W1908114653","https://openalex.org/W1908585429","https://openalex.org/W1909542718","https://openalex.org/W1920534944","https://openalex.org/W1938490552","https://openalex.org/W1940436875","https://openalex.org/W1963874808","https://openalex.org/W1964292121","https://openalex.org/W1968772705","https://openalex.org/W1969060808","https://openalex.org/W1972432824","https://openalex.org/W1974160405","https://openalex.org/W1975183148","https://openalex.org/W1976949015","https://openalex.org/W1978442280","https://openalex.org/W1981119799","https://openalex.org/W1982243317","https://openalex.org/W1982873906","https://openalex.org/W1982993081","https://openalex.org/W1986080062","https://openalex.org/W1987009053","https://openalex.org/W1987064733","https://openalex.org/W1988821469","https://openalex.org/W1989418680","https://openalex.org/W1991039509","https://openalex.org/W1991296029","https://openalex.org/W1992764122","https://openalex.org/W1994007675","https://openalex.org/W1994207899","https://openalex.org/W1999280598","https://openalex.org/W2005877813","https://openalex.org/W2006375756","https://openalex.org/W2007064404","https://openalex.org/W2008632546","https://openalex.org/W2009180114","https://openalex.org/W2010990082","https://openalex.org/W2011001509","https://openalex.org/W2011444292","https://openalex.org/W2012302404","https://openalex.org/W2013433351","https://openalex.org/W2013612878","https://openalex.org/W2015391531","https://openalex.org/W2015564773","https://openalex.org/W2016075837","https://openalex.org/W2016469736","https://openalex.org/W2019076201","https://openalex.org/W2019509230","https://openalex.org/W2020324676","https://openalex.org/W2022941219","https://openalex.org/W2029041674","https://openalex.org/W2031850112","https://openalex.org/W2032054300","https://openalex.org/W2035915822","https://openalex.org/W2036208778","https://openalex.org/W2036889501","https://openalex.org/W2039048991","https://openalex.org/W2041055869","https://openalex.org/W2041681452","https://openalex.org/W2043104518","https://openalex.org/W2044388069","https://openalex.org/W2047334595","https://openalex.org/W2047390883","https://openalex.org/W2049849193","https://openalex.org/W2050498090","https://openalex.org/W2051010986","https://openalex.org/W2051891646","https://openalex.org/W2053756634","https://openalex.org/W2055164922","https://openalex.org/W2058525931","https://openalex.org/W2058802180","https://openalex.org/W2058861773","https://openalex.org/W2059073584","https://openalex.org/W2060035942","https://openalex.org/W2060373561","https://openalex.org/W2061040884","https://openalex.org/W2063416091","https://openalex.org/W2066006292","https://openalex.org/W2066109987","https://openalex.org/W2069401930","https://openalex.org/W2069734146","https://openalex.org/W2070042354","https://openalex.org/W2070196678","https://openalex.org/W2070330324","https://openalex.org/W2070760655","https://openalex.org/W2071055102","https://openalex.org/W2072687304","https://openalex.org/W2072874326","https://openalex.org/W2073148907","https://openalex.org/W2077920529","https://openalex.org/W2078349645","https://openalex.org/W2078737933","https://openalex.org/W2078840098","https://openalex.org/W2081343196","https://openalex.org/W2082176639","https://openalex.org/W2082576063","https://openalex.org/W2085277871","https://openalex.org/W2085609136","https://openalex.org/W2087385198","https://openalex.org/W2087477235","https://openalex.org/W2087531418","https://openalex.org/W2089626841","https://openalex.org/W2090201814","https://openalex.org/W2091457898","https://openalex.org/W2093477805","https://openalex.org/W2094203715","https://openalex.org/W2094631665","https://openalex.org/W2096976702","https://openalex.org/W2097060692","https://openalex.org/W2098924735","https://openalex.org/W2103378158","https://openalex.org/W2104017463","https://openalex.org/W2105506618","https://openalex.org/W2111683404","https://openalex.org/W2112011109","https://openalex.org/W2112791784","https://openalex.org/W2113156278","https://openalex.org/W2113929513","https://openalex.org/W2118752479","https://openalex.org/W2120218267","https://openalex.org/W2121222778","https://openalex.org/W2121303975","https://openalex.org/W2124047990","https://openalex.org/W2128635872","https://openalex.org/W2129170338","https://openalex.org/W2132067013","https://openalex.org/W2133067229","https://openalex.org/W2133779970","https://openalex.org/W2135747052","https://openalex.org/W2136728068","https://openalex.org/W2145307718","https://openalex.org/W2146766740","https://openalex.org/W2146890885","https://openalex.org/W2147990023","https://openalex.org/W2149401581","https://openalex.org/W2151777569","https://openalex.org/W2151800393","https://openalex.org/W2152105575","https://openalex.org/W2157566126","https://openalex.org/W2158105833","https://openalex.org/W2160355231","https://openalex.org/W2161407287","https://openalex.org/W2161781971","https://openalex.org/W2165653430","https://openalex.org/W2166125670","https://openalex.org/W2166683510","https://openalex.org/W2169251053","https://openalex.org/W2169821755","https://openalex.org/W2171941574","https://openalex.org/W2279147100","https://openalex.org/W2293410490","https://openalex.org/W2473662885","https://openalex.org/W2613808226"],"related_works":["https://openalex.org/W2145307718","https://openalex.org/W2395393595","https://openalex.org/W2970050373","https://openalex.org/W2006195528","https://openalex.org/W2010990082","https://openalex.org/W141722745","https://openalex.org/W2159900307","https://openalex.org/W561109402","https://openalex.org/W2635795234","https://openalex.org/W1965135816"],"abstract_inverted_index":{"Increased":[0],"emergence":[1],"of":[2,23,48,56,75,91,105,147,222,237,264,300,328],"antibiotic":[3,26,49],"resistance":[4,27,54,93,225],"in":[5,29,45,72,109,205,315],"bacterial":[6],"pathogens":[7],"has":[8],"posed":[9],"a":[10,34,42,60,135,190,219,303,329],"serious":[11],"threat":[12],"to":[13,17,80,86,101,133,248,280],"human":[14],"health.":[15],"Due":[16],"similar":[18],"structural":[19,125],"and":[20,25,67,70,98,117,126,150,163,235,241,244,255,259,294,318],"functional":[21,195],"characteristics":[22],"metal":[24,38,76,92,112,196,214,224,284,290,316],"systems":[28,226],"gram-negative":[30],"bacteria,":[31],"there":[32],"is":[33,200,218,257,326],"growing":[35],"concern":[36],"that":[37,63,143,168,184,250,320,327],"contamination":[39],"functions":[40,71],"as":[41,139,302],"selective":[43],"agent":[44],"the":[46,65,73,78,81,88,95,103,106,145,148,233,251,262,272,283,298,321],"proliferation":[47],"resistance.":[50],"The":[51,211],"CusCFBA":[52],"copper/silver":[53],"system":[55,97],"Escherichia":[57],"coli":[58],"forms":[59],"protein":[61,142,216],"complex":[62],"spans":[64],"inner":[66,149],"outer":[68,151],"membranes":[69],"efflux":[74],"from":[77],"periplasm":[79],"extracellular":[82],"space.":[83],"In":[84,305],"order":[85],"understand":[87],"molecular":[89],"details":[90],"by":[94,198],"Cus":[96,265],"more":[99],"specifically,":[100],"define":[102],"role":[104,138,299,312,323],"periplasmic":[107,213],"components":[108,263],"CBA":[110],"type":[111],"transporters,":[113],"I":[114,165],"characterized":[115],"CusB":[116,129,169,188,199,256,273,293,314],"probed":[118],"its":[119],"interactions":[120],"with":[121,172,207,282],"CusF":[122,217,240,254,277,301,325],"using":[123,175],"various":[124],"biochemical":[127],"tools.":[128],"was":[130,278],"previously":[131],"thought":[132],"play":[134],"relatively":[136],"passive":[137],"an":[140,228,310],"adaptor":[141],"stabilized":[144],"association":[146],"membrane":[152],"proteins.":[153],"Through":[154],"isothermal":[155],"titration":[156],"calorimetry":[157],"(ITC),":[158],"X-ray":[159],"absorption":[160],"spectroscopy":[161],"(XAS),":[162],"mutagenesis,":[164],"have":[166],"shown":[167],"binds":[170],"Cu(I)/Ag(I)":[171],"high":[173],"affinity":[174],"three":[176],"conserved":[177],"methionines.":[178],"Gel":[179],"filtration":[180],"chromatography":[181],"experiments":[182,288],"showed":[183],"upon":[185],"binding":[186,197,215,285],"Ag(I),":[187],"undergoes":[189],"substantial":[191],"conformational":[192],"change.":[193],"Importantly,":[194],"essential":[201],"for":[202,261,313,324,331],"cell":[203],"survival":[204],"environments":[206],"elevated":[208],"copper":[209],"concentrations.":[210],"small":[212],"unique":[220],"component":[221],"monovalent":[223],"serving":[227],"unknown":[229],"function.":[230],"To":[231],"determine":[232],"nature":[234],"specificity":[236],"interaction":[238,252,274],"between":[239,253,292],"CusB,":[242],"ITC":[243],"NMR":[245,268],"were":[246],"used":[247],"show":[249],"metal-dependent":[258],"specific":[260],"system.":[266],"From":[267],"chemical":[269],"shift":[270],"perturbations,":[271],"face":[275],"on":[276],"determined":[279],"overlap":[281],"site.":[286],"XAS":[287],"demonstrate":[289,309],"transfer":[291],"CusF,":[295],"which":[296],"supports":[297],"metallochaperone.":[304],"summary,":[306],"these":[307],"findings":[308],"active":[311],"resistance,":[317],"suggest":[319],"plausible":[322],"metallochaperone":[330],"CusB.":[332]},"counts_by_year":[],"updated_date":"2025-11-06T04:12:42.849631","created_date":"2016-06-24T00:00:00"}