{"id":"https://openalex.org/W817604875","doi":null,"title":"Biochemical characterization of an extracellular protease in Serratia proteamaculans isolated from a spider","display_name":"Biochemical characterization of an extracellular protease in Serratia proteamaculans isolated from a spider","publication_year":2004,"publication_date":"2004-01-01","ids":{"openalex":"https://openalex.org/W817604875","mag":"817604875"},"language":"en","primary_location":{"id":"pmh:oai:http://repository.kribb.re.kr:8080/:201005/6790","doi":null,"is_oa":false,"landing_page_url":"http://hdl.handle.net/201005/6790","pdf_url":null,"source":{"id":"https://openalex.org/S4406922719","display_name":"KRIBB Repository","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"/dspace/upload/original/5667.pdf","raw_type":"Article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5050424210","display_name":"Ki\u2010Eun Lee","orcid":"https://orcid.org/0000-0002-1466-1905","observed_orcids":["https://orcid.org/0000-0002-1466-1905"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Ki\u2010Eun Lee","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5073635203","display_name":"Kim Chul-Hee","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Kim Chul-Hee","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5047557935","display_name":"Hyun-Jung Kwon","orcid":"https://orcid.org/0000-0002-3677-429X","observed_orcids":["https://orcid.org/0000-0002-3677-429X"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Hyun-Jung Kwon","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5103678689","display_name":"Jangyul Kwak","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Jangyul Kwak","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5046317874","display_name":"Dong\u2010Ha Shin","orcid":"https://orcid.org/0000-0001-6171-6166","observed_orcids":["https://orcid.org/0000-0001-6171-6166"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Shin Dong\u2010Ha","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5074924741","display_name":"Doo\u2010Sang Park","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Doo\u2010Sang Park","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5104361489","display_name":"Bae Kyung-Sook","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Bae Kyung-Sook","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5055388143","display_name":"Ho-Yong Park","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Ho-Yong Park","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.2681,"has_fulltext":false,"cited_by_count":7,"citation_normalized_percentile":{"value":0.51634153,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":87,"max":96},"biblio":{"volume":"40","issue":"4","first_page":"269","last_page":"274"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.9839000105857849,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.9839000105857849,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10620","display_name":"Protease and Inhibitor Mechanisms","score":0.0052999998442828655,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.0017999999690800905,"subfield":{"id":"https://openalex.org/subfields/1315","display_name":"Structural Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/serratia-proteamaculans","display_name":"Serratia proteamaculans","score":0.972000002861023},{"id":"https://openalex.org/keywords/extracellular-protease","display_name":"extracellular protease","score":0.9919999837875366},{"id":"https://openalex.org/keywords/metalloproteinases","display_name":"metalloproteinases","score":0.9430000185966492},{"id":"https://openalex.org/keywords/protease-purification","display_name":"protease purification","score":0.7950000166893005},{"id":"https://openalex.org/keywords/enzyme-characterization","display_name":"enzyme characterization","score":0.625},{"id":"https://openalex.org/keywords/1-10-phenanthroline-inhibition","display_name":"1,10-phenanthroline inhibition","score":0.8939999938011169},{"id":"https://openalex.org/keywords/edta-inhibition","display_name":"EDTA inhibition","score":0.828000009059906},{"id":"https://openalex.org/keywords/zymography","display_name":"zymography","score":0.6729999780654907}],"concepts":[{"id":"https://openalex.org/C2776714187","wikidata":"https://www.wikidata.org/wiki/Q212410","display_name":"Protease","level":3,"score":0.6865590810775757},{"id":"https://openalex.org/C177430391","wikidata":"https://www.wikidata.org/wiki/Q1173648","display_name":"Zymography","level":3,"score":0.6700558662414551},{"id":"https://openalex.org/C182220744","wikidata":"https://www.wikidata.org/wiki/Q7251226","display_name":"Proteases","level":3,"score":0.6036596298217773},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.6000979542732239},{"id":"https://openalex.org/C28406088","wikidata":"https://www.wikidata.org/wiki/Q5571762","display_name":"Extracellular","level":2,"score":0.5845261812210083},{"id":"https://openalex.org/C2909400048","wikidata":"https://www.wikidata.org/wiki/Q411575","display_name":"Phenylmethylsulfonyl Fluoride","level":4,"score":0.543266773223877},{"id":"https://openalex.org/C3019223276","wikidata":"https://www.wikidata.org/wiki/Q212410","display_name":"Proteolytic enzymes","level":3,"score":0.5260607600212097},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.5249164700508118},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.49757054448127747},{"id":"https://openalex.org/C55728118","wikidata":"https://www.wikidata.org/wiki/Q425402","display_name":"Metalloproteinase","level":3,"score":0.42476963996887207},{"id":"https://openalex.org/C193363816","wikidata":"https://www.wikidata.org/wiki/Q1617865","display_name":"Size-exclusion chromatography","level":3,"score":0.4180993437767029},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4026297330856323},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.4004940390586853}],"mesh":[],"locations_count":3,"locations":[{"id":"pmh:oai:http://repository.kribb.re.kr:8080/:201005/6790","doi":null,"is_oa":false,"landing_page_url":"http://hdl.handle.net/201005/6790","pdf_url":null,"source":{"id":"https://openalex.org/S4406922719","display_name":"KRIBB Repository","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"/dspace/upload/original/5667.pdf","raw_type":"Article"},{"id":"pmh:oai:https://repository.kribb.re.kr:201005/6790","doi":null,"is_oa":false,"landing_page_url":"https://repository.kribb.re.kr/handle/201005/6790","pdf_url":null,"source":{"id":"https://openalex.org/S4406922719","display_name":"KRIBB Repository","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"/dspace/upload/original/5667.pdf","raw_type":"Article"},{"id":"mag:817604875","doi":null,"is_oa":false,"landing_page_url":"https://www.kci.go.kr/kciportal/ci/sereArticleSearch/ciSereArtiView.kci?sereArticleSearchBean.artiId=ART001096022","pdf_url":null,"source":{"id":"https://openalex.org/S4306494981","display_name":"\ubbf8\uc0dd\ubb3c\ud559\ud68c\uc9c0","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"Korean Journal of Microbiology","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W1756647744","https://openalex.org/W2050857190","https://openalex.org/W2285248396","https://openalex.org/W2004867814","https://openalex.org/W30285558","https://openalex.org/W2166759385","https://openalex.org/W2015002996","https://openalex.org/W2464888120","https://openalex.org/W2551414021","https://openalex.org/W4232233551"],"abstract_inverted_index":{"Serratia":[0],"proteamaculans":[1],"isolated":[2],"from":[3,61],"the":[4,13,18,28,37,62,97,119,130],"midgut":[5],"of":[6,27,45,96,121],"a":[7,49,78,133],"spider":[8],"formed":[9],"big":[10],"halos":[11],"around":[12],"bacterial":[14,19],"colonies,":[15],"indicating":[16],"that":[17,36,129],"strain":[20],"produces":[21],"an":[22,41],"extracellular":[23,29],"protease.":[24],"Activity":[25],"staining":[26],"protein":[30,39],"fractions":[31],"using":[32],"zymogram":[33],"also":[34,115],"demonstrated":[35],"major":[38],"with":[40],"estimated":[42],"molecular":[43],"mass":[44],"52":[46],"kDa":[47],"contained":[48],"high":[50,80],"proteolytic":[51,81,94],"activity.":[52],"The":[53,74,93,113],"protease":[54],"was":[55,99,116],"purified":[56,75],"to":[57],"near":[58],"electrophoretic":[59],"homogeneity":[60],"culture":[63],"supernatant":[64],"after":[65],"filtration":[66],"and":[67,70,86,88,111,123],"ion":[68],"exchange":[69],"size":[71],"exclusion":[72],"chromatography.":[73],"enzyme":[76,98,131],"had":[77],"relatively":[79],"activity":[82,95,114],"between":[83],"pH":[84],"6.0":[85],"10.0":[87],"at":[89],"broad":[90],"temperature":[91],"range.":[92],"not":[100],"inhibited":[101,107],"by":[102,108],"phenylmethylsulfonyl":[103],"fluoride":[104],"but":[105],"strongly":[106],"1,":[109],"10-phenanthroline":[110],"EDTA.":[112],"dependent":[117],"on":[118],"presence":[120],"Ca++":[122],"Zn++":[124],"ions.":[125],"These":[126],"observations":[127],"indicate":[128],"is":[132],"metalloprotease.":[134]},"counts_by_year":[{"year":2021,"cited_by_count":1},{"year":2018,"cited_by_count":2},{"year":2014,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}