{"id":"https://openalex.org/W81333221","doi":"https://doi.org/10.2144/98253st02","title":"Rapid Acquisition of Unknown DNA Sequence Adjacent to a Known Segment by Multiplex Restriction Site PCR","display_name":"Rapid Acquisition of Unknown DNA Sequence Adjacent to a Known Segment by Multiplex Restriction Site PCR","publication_year":1998,"publication_date":"1998-09-01","ids":{"openalex":"https://openalex.org/W81333221","doi":"https://doi.org/10.2144/98253st02","mag":"81333221","pmid":"https://pubmed.ncbi.nlm.nih.gov/9762438"},"language":"en","primary_location":{"id":"doi:10.2144/98253st02","doi":"https://doi.org/10.2144/98253st02","is_oa":true,"landing_page_url":"https://doi.org/10.2144/98253st02","pdf_url":null,"source":{"id":"https://openalex.org/S79990748","display_name":"BioTechniques","issn_l":"0736-6205","issn":["0736-6205","1940-9818"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","jufo-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320316","host_organization_name":"Future Science Ltd","host_organization_lineage":["https://openalex.org/P4310320316"],"host_organization_lineage_names":["Future Science Ltd"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BioTechniques","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://doi.org/10.2144/98253st02","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5074097376","display_name":"KRISTY L. WEBER","orcid":"https://orcid.org/0000-0001-8021-011X","observed_orcids":["https://orcid.org/0000-0001-8021-011X","https://orcid.org/0009-0007-6941-8885"]},"institutions":[{"id":"https://openalex.org/I1330342723","display_name":"Mayo Clinic","ror":"https://ror.org/02qp3tb03","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1330342723"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Kristy L. Weber","raw_affiliation_strings":["Mayo Clinic, Rochester, MN, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Mayo Clinic, Rochester, MN, USA","institution_ids":["https://openalex.org/I1330342723"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5077089290","display_name":"Mark E. Bolander","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1330342723","display_name":"Mayo Clinic","ror":"https://ror.org/02qp3tb03","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1330342723"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Mark E. Bolander","raw_affiliation_strings":["Mayo Clinic, Rochester, MN, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Mayo Clinic, Rochester, MN, USA","institution_ids":["https://openalex.org/I1330342723"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5009037924","display_name":"Gobinda Sarkar","orcid":"https://orcid.org/0000-0002-7879-124X","observed_orcids":["https://orcid.org/0000-0002-7879-124X"]},"institutions":[{"id":"https://openalex.org/I1330342723","display_name":"Mayo Clinic","ror":"https://ror.org/02qp3tb03","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1330342723"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Gobinda Sarkar","raw_affiliation_strings":["Mayo Clinic, Rochester, MN, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Mayo Clinic, Rochester, MN, USA","institution_ids":["https://openalex.org/I1330342723"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I1330342723"],"apc_list":{"value":1415,"currency":"USD","value_usd":1415},"apc_paid":{"value":1415,"currency":"USD","value_usd":1415},"fwci":1.1627,"has_fulltext":false,"cited_by_count":34,"citation_normalized_percentile":{"value":0.75703105,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":93},"biblio":{"volume":"25","issue":"3","first_page":"415","last_page":"419"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.9922000169754028,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.9922000169754028,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10015","display_name":"Genomics and Phylogenetic Studies","score":0.0024999999441206455,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.0012000000569969416,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/nested-pcr","display_name":"nested PCR","score":0.8190000057220459},{"id":"https://openalex.org/keywords/universal-primers","display_name":"universal primers","score":0.6439999938011169},{"id":"https://openalex.org/keywords/restriction-enzyme-sites","display_name":"restriction enzyme sites","score":0.6470000147819519},{"id":"https://openalex.org/keywords/genome-mapping","display_name":"genome mapping","score":0.7350000143051147},{"id":"https://openalex.org/keywords/automated-sequencing","display_name":"automated sequencing","score":0.3799999952316284}],"concepts":[{"id":"https://openalex.org/C2777563447","wikidata":"https://www.wikidata.org/wiki/Q7243397","display_name":"Primer (cosmetics)","level":2,"score":0.7227686047554016},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6774656772613525},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.6534044742584229},{"id":"https://openalex.org/C200658500","wikidata":"https://www.wikidata.org/wiki/Q4112082","display_name":"In silico PCR","level":5,"score":0.6454349160194397},{"id":"https://openalex.org/C90583042","wikidata":"https://www.wikidata.org/wiki/Q1952456","display_name":"Multiplex polymerase chain reaction","level":4,"score":0.5762548446655273},{"id":"https://openalex.org/C206625514","wikidata":"https://www.wikidata.org/wiki/Q2068713","display_name":"Sequence-tagged site","level":5,"score":0.5584417581558228},{"id":"https://openalex.org/C2781188995","wikidata":"https://www.wikidata.org/wiki/Q6934982","display_name":"Multiplex","level":2,"score":0.5557952523231506},{"id":"https://openalex.org/C128319531","wikidata":"https://www.wikidata.org/wiki/Q219715","display_name":"Restriction enzyme","level":3,"score":0.5533228516578674},{"id":"https://openalex.org/C2778112365","wikidata":"https://www.wikidata.org/wiki/Q3511065","display_name":"Sequence (biology)","level":2,"score":0.5450795292854309},{"id":"https://openalex.org/C58301748","wikidata":"https://www.wikidata.org/wiki/Q1074969","display_name":"Restriction site","level":4,"score":0.5415125489234924},{"id":"https://openalex.org/C17757408","wikidata":"https://www.wikidata.org/wiki/Q4135516","display_name":"genomic DNA","level":3,"score":0.5104009509086609},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.5101400017738342},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.4838499128818512},{"id":"https://openalex.org/C61053724","wikidata":"https://www.wikidata.org/wiki/Q1154615","display_name":"Sequence analysis","level":3,"score":0.4650840163230896},{"id":"https://openalex.org/C2889275","wikidata":"https://www.wikidata.org/wiki/Q7316313","display_name":"Restriction digest","level":4,"score":0.4394468665122986},{"id":"https://openalex.org/C141231307","wikidata":"https://www.wikidata.org/wiki/Q7020","display_name":"Genome","level":3,"score":0.43138349056243896},{"id":"https://openalex.org/C84597430","wikidata":"https://www.wikidata.org/wiki/Q106227","display_name":"Locus (genetics)","level":3,"score":0.4281519055366516},{"id":"https://openalex.org/C51679486","wikidata":"https://www.wikidata.org/wiki/Q380546","display_name":"DNA sequencing","level":3,"score":0.4215629994869232},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.29940497875213623},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.22668614983558655},{"id":"https://openalex.org/C122735190","wikidata":"https://www.wikidata.org/wiki/Q715789","display_name":"Gene mapping","level":4,"score":0.12052184343338013},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0},{"id":"https://openalex.org/C30481170","wikidata":"https://www.wikidata.org/wiki/Q37748","display_name":"Chromosome","level":3,"score":0.0},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.0}],"mesh":[{"descriptor_ui":"D001665","descriptor_name":"Binding Sites","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D004262","descriptor_name":"DNA Restriction Enzymes","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D004273","descriptor_name":"DNA, Neoplasm","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004273","descriptor_name":"DNA, Neoplasm","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015245","descriptor_name":"Deoxyribonuclease BamHI","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D015246","descriptor_name":"Deoxyribonuclease EcoRI","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D015252","descriptor_name":"Deoxyribonucleases, Type II Site-Specific","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D016133","descriptor_name":"Polymerase Chain Reaction","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D017422","descriptor_name":"Sequence Analysis, DNA","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017931","descriptor_name":"DNA Primers","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.2144/98253st02","doi":"https://doi.org/10.2144/98253st02","is_oa":true,"landing_page_url":"https://doi.org/10.2144/98253st02","pdf_url":null,"source":{"id":"https://openalex.org/S79990748","display_name":"BioTechniques","issn_l":"0736-6205","issn":["0736-6205","1940-9818"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","jufo-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320316","host_organization_name":"Future Science Ltd","host_organization_lineage":["https://openalex.org/P4310320316"],"host_organization_lineage_names":["Future Science Ltd"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BioTechniques","raw_type":"journal-article"},{"id":"pmid:9762438","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/9762438","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BioTechniques","raw_type":"Research Support, Non-U.S. Gov't"},{"id":"pmh:oai:doaj.org/article:056fe16eedf541f99d6eb6766721dacb","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/056fe16eedf541f99d6eb6766721dacb","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"BioTechniques, Vol 25, Iss 3, Pp 415-419 (1998)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.2144/98253st02","doi":"https://doi.org/10.2144/98253st02","is_oa":true,"landing_page_url":"https://doi.org/10.2144/98253st02","pdf_url":null,"source":{"id":"https://openalex.org/S79990748","display_name":"BioTechniques","issn_l":"0736-6205","issn":["0736-6205","1940-9818"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","jufo-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320316","host_organization_name":"Future Science Ltd","host_organization_lineage":["https://openalex.org/P4310320316"],"host_organization_lineage_names":["Future Science Ltd"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BioTechniques","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":6,"referenced_works":["https://openalex.org/W1969304546","https://openalex.org/W1996032544","https://openalex.org/W2086794780","https://openalex.org/W2128750289","https://openalex.org/W2170651165","https://openalex.org/W2336534018"],"related_works":["https://openalex.org/W2170651165","https://openalex.org/W7101092641","https://openalex.org/W2145676420","https://openalex.org/W2083369461","https://openalex.org/W1564541111","https://openalex.org/W2008282869","https://openalex.org/W2048261706","https://openalex.org/W4254457236","https://openalex.org/W4214779817","https://openalex.org/W2116278708"],"abstract_inverted_index":{"The":[0,61,140],"determination":[1],"of":[2,73,87,171,176,180,188,199,206],"unknown":[3,154,207],"DNA":[4],"sequences":[5,159],"around":[6],"a":[7,44,81,117,185,212],"known":[8,46,89,158,186,213],"locus":[9],"has":[10],"important":[11],"applications":[12],"in":[13,17,160,173],"molecular":[14],"genetics,":[15],"specifically":[16],"genomic":[18,181],"walking":[19],"and":[20,80,125,148,164],"genome":[21],"mapping.":[22],"Several":[23],"PCR-based":[24],"methods":[25,201],"have":[26,41,92],"been":[27],"reported":[28],"to":[29,152,157,195,211],"address":[30,203],"this":[31],"issue,":[32],"but":[33],"they":[34],"often":[35],"involve":[36],"multiple,":[37],"time-consuming":[38],"steps.":[39],"We":[40,91,167],"previously":[42],"described":[43],"technique":[45,97],"as":[47],"restriction":[48,75],"site":[49],"PCR":[50,64,106,119],"(RS-PCR)":[51],"that":[52,70,202],"allows":[53],"sequence":[54,155,182,187,208],"acquisition":[55,175],"faster":[56],"than":[57],"the":[58,88,96,100,109,121,132,162,169,174,197,204],"existing":[59],"methods.":[60],"method":[62],"involves":[63],"using":[65],"four":[66,101],"separate":[67],"universal":[68,102],"primers":[69,103,124],"are":[71],"representative":[72],"given":[74],"enzyme":[76],"sites":[77],"(RS":[78],"primers),":[79],"specific":[82,111,128],"primer":[83],"from":[84,184],"one":[85,105],"end":[86],"sequence.":[90],"now":[93],"significantly":[94],"improved":[95],"by":[98,116,136],"mixing":[99],"into":[104],"tube":[107],"with":[108,120],"first":[110],"primer.":[112],"This":[113],"is":[114,134,146],"followed":[115],"nested":[118],"mixed":[122],"RS":[123],"an":[126],"internal":[127],"primer,":[129],"after":[130],"which":[131],"product":[133],"sequenced":[135],"direct":[137],"automated":[138],"sequencing.":[139],"technique,":[141],"called":[142],"multiplex":[143],"RS-PCR":[144,193],"(mRS-PCR),":[145],"reproducible":[147],"can":[149],"be":[150,196],"used":[151],"obtain":[153],"adjacent":[156,210],"both":[161],"upstream":[163],"downstream":[165],"directions.":[166],"illustrate":[168],"application":[170],"mRS-PCR":[172],"approximately":[177,189],"780":[178],"bp":[179],"starting":[183],"120":[190],"bp.":[191],"Multiplex":[192],"appears":[194],"fastest":[198],"all":[200],"issue":[205],"retrieval":[209],"region.":[214]},"counts_by_year":[{"year":2018,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}