{"id":"https://openalex.org/W80428576","doi":"https://doi.org/10.1007/978-1-59745-522-0_3","title":"Methyl Group Acceptance Assay for the Determination of Global DNA Methylation Levels","display_name":"Methyl Group Acceptance Assay for the Determination of Global DNA Methylation Levels","publication_year":2008,"publication_date":"2008-11-05","ids":{"openalex":"https://openalex.org/W80428576","doi":"https://doi.org/10.1007/978-1-59745-522-0_3","mag":"80428576","pmid":"https://pubmed.ncbi.nlm.nih.gov/18987804"},"language":"en","primary_location":{"id":"doi:10.1007/978-1-59745-522-0_3","doi":"https://doi.org/10.1007/978-1-59745-522-0_3","is_oa":false,"landing_page_url":"https://doi.org/10.1007/978-1-59745-522-0_3","pdf_url":null,"source":{"id":"https://openalex.org/S4210172139","display_name":"Methods in molecular biology","issn_l":"1064-3745","issn":["1064-3745","1940-6029"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3","vabb-shw"],"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"book series"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in Molecular Biology","raw_type":"book-chapter"},"volume":{"id":"https://openalex.org/W4238166333","display_name":"DNA Methylation"},"type":"book-chapter","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5061888144","display_name":"Kenneth P. Nephew","orcid":"https://orcid.org/0000-0003-2192-1346","observed_orcids":["https://orcid.org/0000-0003-2192-1346"]},"institutions":[{"id":"https://openalex.org/I4210119109","display_name":"Indiana University Bloomington","ror":"https://ror.org/02k40bc56","country_code":"US","type":"education","lineage":["https://openalex.org/I4210119109","https://openalex.org/I592451"]},{"id":"https://openalex.org/I4394709130","display_name":"Indiana University School of Medicine","ror":"https://ror.org/02ets8c94","country_code":"US","type":"education","lineage":["https://openalex.org/I4394709130","https://openalex.org/I592451"]},{"id":"https://openalex.org/I592451","display_name":"Indiana University","ror":"https://ror.org/01kg8sb98","country_code":"US","type":"education","lineage":["https://openalex.org/I592451"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Kenneth P Nephew","raw_affiliation_strings":["Department of Cellular and Integrative Physiology, Medical Sciences Program, Indiana University School of Medicine, Bloomington, IN, USA","Indiana University School of Medicine"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Cellular and Integrative Physiology, Medical Sciences Program, Indiana University School of Medicine, Bloomington, IN, USA","institution_ids":["https://openalex.org/I4210119109","https://openalex.org/I4394709130","https://openalex.org/I592451"]},{"raw_affiliation_string":"Indiana University School of Medicine","institution_ids":["https://openalex.org/I4394709130"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5112036995","display_name":"Curt Balch","orcid":"https://orcid.org/0000-0002-7878-156X","observed_orcids":["https://orcid.org/0000-0002-7878-156X"]},"institutions":[{"id":"https://openalex.org/I4210119109","display_name":"Indiana University Bloomington","ror":"https://ror.org/02k40bc56","country_code":"US","type":"education","lineage":["https://openalex.org/I4210119109","https://openalex.org/I592451"]},{"id":"https://openalex.org/I4394709130","display_name":"Indiana University School of Medicine","ror":"https://ror.org/02ets8c94","country_code":"US","type":"education","lineage":["https://openalex.org/I4394709130","https://openalex.org/I592451"]},{"id":"https://openalex.org/I592451","display_name":"Indiana University","ror":"https://ror.org/01kg8sb98","country_code":"US","type":"education","lineage":["https://openalex.org/I592451"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Curt Balch","raw_affiliation_strings":["Department of Cellular and Integrative Physiology, Medical Sciences Program, Indiana University School of Medicine, Bloomington, USA","Indiana University School of Medicine"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Cellular and Integrative Physiology, Medical Sciences Program, Indiana University School of Medicine, Bloomington, USA","institution_ids":["https://openalex.org/I4210119109","https://openalex.org/I4394709130","https://openalex.org/I592451"]},{"raw_affiliation_string":"Indiana University School of Medicine","institution_ids":["https://openalex.org/I4394709130"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5047360770","display_name":"David G. Skalnik","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4390039234","display_name":"Indiana University Melvin and Bren Simon Comprehensive Cancer Center","ror":"https://ror.org/00g1d7b60","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4390039234","https://openalex.org/I592451"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"David G Skalnik","raw_affiliation_strings":["Indiana University Cancer Center, Indianapolis, USA","Indiana University Cancer Center"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Indiana University Cancer Center, Indianapolis, USA","institution_ids":["https://openalex.org/I4390039234"]},{"raw_affiliation_string":"Indiana University Cancer Center","institution_ids":["https://openalex.org/I4390039234"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":4,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":2.163,"has_fulltext":false,"cited_by_count":13,"citation_normalized_percentile":{"value":0.86078173,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":96},"biblio":{"volume":"507","issue":null,"first_page":"35","last_page":"41"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10269","display_name":"Epigenetics and DNA Methylation","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10269","display_name":"Epigenetics and DNA Methylation","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T13157","display_name":"Cancer-related gene regulation","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11928","display_name":"Genetic Syndromes and Imprinting","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/global-dna-methylation","display_name":"global DNA methylation","score":0.9610000252723694},{"id":"https://openalex.org/keywords/s-adenosylmethionine","display_name":"S-adenosylmethionine","score":0.7820000052452087},{"id":"https://openalex.org/keywords/cpg-methylation","display_name":"CpG methylation","score":0.8880000114440918},{"id":"https://openalex.org/keywords/hypomethylating-agents","display_name":"hypomethylating agents","score":0.8090000152587891},{"id":"https://openalex.org/keywords/myelodysplastic-syndrome","display_name":"myelodysplastic syndrome","score":0.8479999899864197},{"id":"https://openalex.org/keywords/tumor-suppressor-gene-hypermethylation","display_name":"tumor suppressor gene hypermethylation","score":0.8090000152587891},{"id":"https://openalex.org/keywords/genomic-hypomethylation","display_name":"genomic hypomethylation","score":0.8679999709129333}],"concepts":[{"id":"https://openalex.org/C190727270","wikidata":"https://www.wikidata.org/wiki/Q874745","display_name":"DNA methylation","level":4,"score":0.6551550626754761},{"id":"https://openalex.org/C33288867","wikidata":"https://www.wikidata.org/wiki/Q518328","display_name":"Methylation","level":3,"score":0.5764068961143494},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.47873160243034363},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4172058701515198},{"id":"https://openalex.org/C2781311116","wikidata":"https://www.wikidata.org/wiki/Q83306","display_name":"Group (periodic table)","level":2,"score":0.4127030670642853},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.385071337223053},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3140799403190613},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.3121289610862732},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.09398913383483887},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.05573078989982605},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.04324781894683838}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D004273","descriptor_name":"DNA, Neoplasm","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004273","descriptor_name":"DNA, Neoplasm","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D009190","descriptor_name":"Myelodysplastic Syndromes","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D009369","descriptor_name":"Neoplasms","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D009369","descriptor_name":"Neoplasms","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D016147","descriptor_name":"Genes, Tumor Suppressor","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018899","descriptor_name":"CpG Islands","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019175","descriptor_name":"DNA Methylation","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D045744","descriptor_name":"Cell Line, Tumor","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1007/978-1-59745-522-0_3","doi":"https://doi.org/10.1007/978-1-59745-522-0_3","is_oa":false,"landing_page_url":"https://doi.org/10.1007/978-1-59745-522-0_3","pdf_url":null,"source":{"id":"https://openalex.org/S4210172139","display_name":"Methods in molecular biology","issn_l":"1064-3745","issn":["1064-3745","1940-6029"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3","vabb-shw"],"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"book series"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in Molecular Biology","raw_type":"book-chapter"},{"id":"pmid:18987804","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/18987804","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in molecular biology (Clifton, N.J.)","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.5683000087738037,"id":"https://metadata.un.org/sdg/3"}],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G3455293255","display_name":null,"funder_award_id":"CA085289","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"},{"id":"https://openalex.org/G3950809854","display_name":"DNA Methylation and Ovarian Cancer","funder_award_id":"R01CA085289","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"}],"funders":[{"id":"https://openalex.org/F4320337351","display_name":"National Cancer Institute","ror":"https://ror.org/040gcmg81"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":23,"referenced_works":["https://openalex.org/W1564686243","https://openalex.org/W1837304276","https://openalex.org/W1967064280","https://openalex.org/W1967841068","https://openalex.org/W1978971513","https://openalex.org/W1998517881","https://openalex.org/W2005941717","https://openalex.org/W2030774686","https://openalex.org/W2032622444","https://openalex.org/W2033102369","https://openalex.org/W2052211882","https://openalex.org/W2070471476","https://openalex.org/W2075684759","https://openalex.org/W2078028724","https://openalex.org/W2079316438","https://openalex.org/W2082401264","https://openalex.org/W2109881276","https://openalex.org/W2111183991","https://openalex.org/W2113831904","https://openalex.org/W2119615097","https://openalex.org/W2137018736","https://openalex.org/W2146282065","https://openalex.org/W2266990620"],"related_works":["https://openalex.org/W3178194959","https://openalex.org/W2890057816","https://openalex.org/W3034064495","https://openalex.org/W2537518441","https://openalex.org/W7100402649","https://openalex.org/W2083886218","https://openalex.org/W1564686243","https://openalex.org/W2740044387","https://openalex.org/W2071017675","https://openalex.org/W2534415170"],"abstract_inverted_index":{"DNA":[0,35,72,87,96],"methylation":[1,73,88],"levels":[2],"are":[3,38],"affected":[4],"by":[5,67],"numerous":[6],"environmental":[7],"influences,":[8],"including":[9],"diet":[10],"and":[11,14,23,58,144],"xenobiotic":[12],"exposure,":[13],"neoplasia":[15],"has":[16],"been":[17],"firmly":[18],"associated":[19],"with":[20,46],"genomic":[21,86],"hypomethylation":[22],"localized":[24],"hypermethylation":[25],"of":[26,48,55,61,70,85,94,132,142],"tumor":[27],"suppressor":[28],"genes.":[29],"To":[30],"reverse":[31],"methylation-induced":[32],"gene":[33],"repression,":[34],"hypomethylating":[36],"agents":[37],"currently":[39],"in":[40,119],"clinical":[41],"trials":[42],"for":[43,52,82],"various":[44],"malignancies,":[45],"two":[47],"these":[49,62],"now":[50],"approved":[51],"the":[53,59,68,83,92,106,120,130],"therapy":[54],"myelodysplastic":[56],"syndrome,":[57],"efficacy":[60],"drugs":[63],"can":[64],"be":[65],"assessed":[66],"monitoring":[69],"global":[71],"levels.":[74],"Herein,":[75],"we":[76],"outline":[77],"a":[78,140],"simple,":[79],"well-established":[80],"method":[81],"evaluation":[84],"levels,":[89],"based":[90],"on":[91],"ability":[93],"isolated":[95],"to":[97,129,139],"\"accept\"":[98],"radiolabeled":[99,122],"methyl":[100,123],"groups":[101],"from":[102],"S-[3H-methyl]":[103],"adenosylmethionine,":[104],"using":[105],"bacterial":[107],"CpG":[108,117],"methyltransferase":[109],"SssI.":[110],"As":[111],"this":[112],"enzyme":[113],"methylates":[114],"all":[115],"unmethylated":[116],"dinucleotides":[118],"genome,":[121],"group":[124],"acceptance":[125],"is":[126,137],"inversely":[127],"proportional":[128],"level":[131],"preexisting":[133],"methylation.":[134],"This":[135],"assay":[136],"applicable":[138],"number":[141],"translational":[143],"basic":[145],"research":[146],"questions.":[147]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":2},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":1},{"year":2012,"cited_by_count":2}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}