{"id":"https://openalex.org/W79279814","doi":null,"title":"Express\u00e3o e purifica\u00e7\u00e3o da prote\u00edna E3 do v\u00edrus chikungunya (CHIKV)","display_name":"Express\u00e3o e purifica\u00e7\u00e3o da prote\u00edna E3 do v\u00edrus chikungunya (CHIKV)","publication_year":2013,"publication_date":"2013-01-01","ids":{"openalex":"https://openalex.org/W79279814","mag":"79279814"},"language":"pt","primary_location":{"id":"pmh:oai:run.unl.pt:10362/10528","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/10362/10528","pdf_url":"http://hdl.handle.net/10362/10528","source":{"id":"https://openalex.org/S4306402433","display_name":"Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"master thesis"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"http://hdl.handle.net/10362/10528","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5090844510","display_name":"Tatiana Priscilla Coelho Pinto","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Tatiana Priscilla Coelho Pinto","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5090844510"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.6830999851226807,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.6830999851226807,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10166","display_name":"Mosquito-borne diseases and control","score":0.2808000147342682,"subfield":{"id":"https://openalex.org/subfields/2739","display_name":"Public Health, Environmental and Occupational Health"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11663","display_name":"Viral Infectious Diseases and Gene Expression in Insects","score":0.007499999832361937,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/chikungunya-virus","display_name":"chikungunya virus","score":0.968999981880188},{"id":"https://openalex.org/keywords/recombinant-protein-expression","display_name":"recombinant protein expression","score":0.8650000095367432},{"id":"https://openalex.org/keywords/protein-purification","display_name":"protein purification","score":0.9340000152587891},{"id":"https://openalex.org/keywords/chitin-binding-domain","display_name":"chitin-binding domain","score":0.7319999933242798},{"id":"https://openalex.org/keywords/impact-systems","display_name":"impact systems","score":0.6359999775886536},{"id":"https://openalex.org/keywords/nickel-affinity-chromatography","display_name":"nickel affinity chromatography","score":0.7609999775886536},{"id":"https://openalex.org/keywords/histidine-tag","display_name":"histidine tag","score":0.7099999785423279},{"id":"https://openalex.org/keywords/serological-diagnosis","display_name":"serological diagnosis","score":0.47600001096725464},{"id":"https://openalex.org/keywords/togaviridae","display_name":"Togaviridae","score":0.7400000095367432},{"id":"https://openalex.org/keywords/aedes-mosquito-vector","display_name":"Aedes mosquito vector","score":0.46799999475479126},{"id":"https://openalex.org/keywords/arthritis","display_name":"arthritis","score":0.49000000953674316},{"id":"https://openalex.org/keywords/recombinant-antibodies","display_name":"recombinant antibodies","score":0.4230000078678131}],"concepts":[{"id":"https://openalex.org/C2781273456","wikidata":"https://www.wikidata.org/wiki/Q243257","display_name":"Chikungunya","level":3,"score":0.8726754188537598},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.5788411498069763},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4085802435874939},{"id":"https://openalex.org/C533803919","wikidata":"https://www.wikidata.org/wiki/Q30953","display_name":"Dengue fever","level":2,"score":0.26441511511802673}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:run.unl.pt:10362/10528","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/10362/10528","pdf_url":"http://hdl.handle.net/10362/10528","source":{"id":"https://openalex.org/S4306402433","display_name":"Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"master thesis"},{"id":"mag:79279814","doi":null,"is_oa":false,"landing_page_url":"https://run.unl.pt/bitstream/10362/10528/1/Pinto%20Tatiana%20TM%202013.pdf","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"pmh:oai:run.unl.pt:10362/10528","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/10362/10528","pdf_url":"http://hdl.handle.net/10362/10528","source":{"id":"https://openalex.org/S4306402433","display_name":"Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"master thesis"},"sustainable_development_goals":[{"score":0.8371999859809875,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W79279814.pdf","grobid_xml":"https://content.openalex.org/works/W79279814.grobid-xml"},"referenced_works_count":91,"referenced_works":["https://openalex.org/W188244135","https://openalex.org/W201979082","https://openalex.org/W1456773773","https://openalex.org/W1525285670","https://openalex.org/W1551390803","https://openalex.org/W1554214469","https://openalex.org/W1565757498","https://openalex.org/W1569091428","https://openalex.org/W1602528206","https://openalex.org/W1667933161","https://openalex.org/W1700316245","https://openalex.org/W1835852057","https://openalex.org/W1912423637","https://openalex.org/W1931534490","https://openalex.org/W1963590629","https://openalex.org/W1963938989","https://openalex.org/W1969557290","https://openalex.org/W1973562680","https://openalex.org/W1974677665","https://openalex.org/W1977762935","https://openalex.org/W1991353963","https://openalex.org/W1999780961","https://openalex.org/W2000148277","https://openalex.org/W2006232166","https://openalex.org/W2007151296","https://openalex.org/W2007153351","https://openalex.org/W2008108263","https://openalex.org/W2009750207","https://openalex.org/W2010444790","https://openalex.org/W2010546547","https://openalex.org/W2014298634","https://openalex.org/W2016270731","https://openalex.org/W2016636607","https://openalex.org/W2016858079","https://openalex.org/W2017919672","https://openalex.org/W2020293816","https://openalex.org/W2023092952","https://openalex.org/W2026596261","https://openalex.org/W2026949398","https://openalex.org/W2027926218","https://openalex.org/W2028357619","https://openalex.org/W2029116599","https://openalex.org/W2032559848","https://openalex.org/W2035039373","https://openalex.org/W2035587893","https://openalex.org/W2036626025","https://openalex.org/W2043999806","https://openalex.org/W2046391048","https://openalex.org/W2049449836","https://openalex.org/W2051661439","https://openalex.org/W2055274364","https://openalex.org/W2056269653","https://openalex.org/W2060823949","https://openalex.org/W2062504884","https://openalex.org/W2064918842","https://openalex.org/W2068012691","https://openalex.org/W2068988851","https://openalex.org/W2069104829","https://openalex.org/W2074236357","https://openalex.org/W2078272057","https://openalex.org/W2083524751","https://openalex.org/W2087117638","https://openalex.org/W2090886299","https://openalex.org/W2097446992","https://openalex.org/W2101642792","https://openalex.org/W2103230759","https://openalex.org/W2103460335","https://openalex.org/W2108822951","https://openalex.org/W2113639652","https://openalex.org/W2113728375","https://openalex.org/W2115022310","https://openalex.org/W2117326745","https://openalex.org/W2127003764","https://openalex.org/W2130974624","https://openalex.org/W2131836981","https://openalex.org/W2136373859","https://openalex.org/W2136857202","https://openalex.org/W2140237950","https://openalex.org/W2146545416","https://openalex.org/W2150157806","https://openalex.org/W2151991090","https://openalex.org/W2156735354","https://openalex.org/W2159624858","https://openalex.org/W2170388413","https://openalex.org/W2184405013","https://openalex.org/W2259698181","https://openalex.org/W2390037597","https://openalex.org/W2408727670","https://openalex.org/W2411525666","https://openalex.org/W2413721677","https://openalex.org/W4240701297"],"related_works":["https://openalex.org/W3215485778","https://openalex.org/W2374711163","https://openalex.org/W7119246163","https://openalex.org/W2905386993","https://openalex.org/W2095334831","https://openalex.org/W7065695035","https://openalex.org/W7045886504","https://openalex.org/W2596333243","https://openalex.org/W3157406435","https://openalex.org/W2469657763"],"abstract_inverted_index":{"O":[0],"v\u00edrus":[1,6],"chikungunya":[2],"(CHIKV)":[3],"\u00e9":[4],"um":[5],"de":[7,23,30,35,49,63,83,87,94,98,111,121,138,149,155,169,172,187,191,196,201,239,253,258,261,282,289],"RNA,":[8],"com":[9,37,135,213,236,265,274,291,297,300],"inv\u00f3lucro,":[10],"da":[11,55,133,208,227,280],"fam\u00edlia":[12],"Togaviridae,":[13],"transmitido":[14],"por":[15,20,57,180,243],"mosquitos":[16],"Aedes":[17],"spp.":[18],"Distribu\u00eddo":[19],"largas":[21],"regi\u00f5es":[22],"\u00c1frica":[24],"e":[25,43,46,107,161,219,294],"\u00c1sia,":[26],"causa":[27],"grandes":[28],"epidemias":[29],"artrite":[31],"grave.":[32],"A":[33,59,146,175,189,278],"semelhan\u00e7a":[34],"sintomas":[36],"outras":[38],"doen\u00e7as":[39],"como":[40,166],"a":[41,44,47,69,92,153,177,185,199,206,221,256,266],"dengue":[42],"mal\u00e1ria":[45],"persist\u00eancia":[48],"IgM":[50],"espec\u00edficas,":[51],"dificultam":[52],"o":[53,76,136],"diagn\u00f3stico":[54,77],"infe\u00e7\u00e3o":[56],"CHIKV.":[58,301],"dete\u00e7\u00e3o":[60],"no":[61,245],"sangue":[62],"E3,":[64],"uma":[65,167,237,259],"glicoprote\u00edna":[66],"viral":[67],"secretada,":[68],"incluir":[70],"num":[71,96,124],"ensaio":[72,97],"imunoenzim\u00e1tico":[73],"poder\u00e1":[74],"melhorar":[75],"nos":[78],"pa\u00edses":[79],"onde":[80],"as":[81],"t\u00e9cnicas":[82],"biologia":[84],"molecular":[85,268],"s\u00e3o":[86],"dif\u00edcil":[88],"acesso.":[89],"Para":[90,114],"testar":[91],"utilidade":[93],"E3":[95,116,150,223,229],"diagn\u00f3stico,":[99],"esta":[100,292],"dever\u00e1":[101],"ser":[102,122],"expressa":[103,162,232],"em":[104,159,163,194,233,251],"quantidade,":[105],"purificada":[106,123],"usada":[108],"para":[109,270],"produ\u00e7\u00e3o":[110],"anticorpos":[112,290],"espec\u00edficos.":[113],"expressar":[115],"numa":[117],"forma":[118],"sol\u00favel,":[119],"suscet\u00edvel":[120],"\u00fanico":[125],"passo":[126],"cromatogr\u00e1fico":[127],"sem":[128],"proteases,":[129],"recorreu-se":[130],"\u00e0":[131,140],"estrat\u00e9gia":[132],"fus\u00e3o":[134,170],"dom\u00ednio":[137],"liga\u00e7\u00e3o":[139],"quitina":[141,197],"(CBD)-inte\u00edna":[142],"(IMPACT\u2122":[143],"System,":[144],"NEB).":[145],"sequ\u00eancia":[147],"codificadora":[148],"foi":[151,216,230],"amplificada":[152],"partir":[154],"RNA":[156],"viral,":[157],"clonada":[158],"pTYB21":[160],"E.":[164,234],"coli":[165,235],"prote\u00edna":[168,222,260,281],"insol\u00favel":[171],"64":[173],"kDa.":[174],"express\u00e3o":[176],"12\u00baC":[178],"induzida":[179,212],"IPTG":[181],"0,1":[182],"mM":[183],"aumentou":[184],"solubilidade":[186],"CBD-inte\u00edna-E3.":[188],"aplica\u00e7\u00e3o":[190],"lisados":[192],"celulares":[193,249],"colunas":[195,252],"originou":[198],"reten\u00e7\u00e3o":[200],"CBD-inte\u00edna-E3":[202],"na":[203,210],"matriz.":[204],"Por\u00e9m,":[205],"autoclivagem":[207],"inte\u00edna":[209],"coluna,":[211],"reagentes":[214],"tiol,":[215],"pouco":[217],"eficiente":[218],"mesmo":[220],"separada":[224],"n\u00e3o":[225],"eluiu":[226],"coluna.":[228],"ainda":[231,272],"cauda":[238],"seis":[240],"histidinas":[241],"(E3[His]6)":[242],"clonagem":[244],"vetor":[246],"pET28b(+).":[247],"Lisados":[248],"aplicados":[250],"n\u00edquel":[254],"permitiram":[255],"elui\u00e7\u00e3o":[257],"9":[262,283],"kDa,":[263],"compat\u00edvel":[264],"massa":[267],"estimada":[269],"E3[His]6,":[271],"que":[273],"outros":[275],"contaminantes":[276],"proteicos.":[277],"identidade":[279],"kDa":[284],"ser\u00e1":[285],"confirmada":[286],"pela":[287],"indu\u00e7\u00e3o":[288],"prepara\u00e7\u00e3o":[293],"reatividade":[295],"daqueles":[296],"c\u00e9lulas":[298],"infetadas":[299]},"counts_by_year":[],"updated_date":"2026-11-08T12:12:28.789561","created_date":"2016-06-24T00:00:00"}
